MIP_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MIP_HUMAN
• UniProt AC: P30301
• Protein Name: Lens fiber major intrinsic protein
• Gene Name: MIP
• Organism: Homo sapiens (Human).
• Sequence Length: 263
• Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Cell junction, gap junction .
• Protein Description: Water channel. [PubMed: 24120416 Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core (By similarity Plays a role in cell-to-cell adhesion and facilitates gap junction coupling]
• Protein Sequence: MWELRSASFWRAIFAEFFATLFYVFFGLGSSLRWAPGPLHVLQVAMAFGLALATLVQSVGHISGAHVNPAVTFAFLVGSQMSLLRAFCYMAAQLLGAVAGAAVLYSVTPPAVRGNLALNTLHPAVSVGQATTVEIFLTLQFVLCIFATYDERRNGQLGSVALAVGFSLALGHLFGMYYTGAGMNPARSFAPAILTGNFTNHWVYWVGPIIGGGLGSLLYDFLLFPRLKSISERLSVLKGAKPDVSNGQPEVTGEPVELNTQAL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Phosphorylation	VFFGLGSSLRWAPGP HHHCCCCCCCCCCCH	21.83	24719451
229	Phosphorylation	LLFPRLKSISERLSV HHHHHHHHHHHHHHH	35.62	2176601
231	Phosphorylation	FPRLKSISERLSVLK HHHHHHHHHHHHHHH	24.61	18081321
235	Phosphorylation	KSISERLSVLKGAKP HHHHHHHHHHHCCCC	31.46	18081321
245	Phosphorylation	KGAKPDVSNGQPEVT HCCCCCCCCCCCCCC	42.49	-
246	Deamidated asparagine	GAKPDVSNGQPEVTG CCCCCCCCCCCCCCC	53.63	-
246	Deamidation	GAKPDVSNGQPEVTG CCCCCCCCCCCCCCC	53.63	10634618
259	Deamidated asparagine	TGEPVELNTQAL--- CCCCEECCCCCC---	19.35	-
259	Deamidation	TGEPVELNTQAL--- CCCCEECCCCCC---	19.35	10634618

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
229	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
229	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
231	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
231	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MIP_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MIP_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CRYAA_HUMAN	CRYAA	physical	18004741
CRYAB_HUMAN	CRYAB	physical	18004741
CRBB2_HUMAN	CRYBB2	physical	18004741
CRGC_HUMAN	CRYGC	physical	18004741

Drug and Disease Associations

• Kegg Disease
• H01202: Cataract
• OMIM Disease
• 615274: Cataract 15, multiple types (CTRCT15)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Deamidation

"Characterization of human lens major intrinsic protein structure.";
Schey K.L., Little M., Fowler J.G., Crouch R.K.;
Invest. Ophthalmol. Vis. Sci. 41:175-182(2000).
Cited for: PHOSPHORYLATION AT SER-235, DEAMIDATION AT ASN-246 AND ASN-259, ANDMASS SPECTROMETRY.
PubMed: 10634618

Phosphorylation

"Characterization of human lens major intrinsic protein structure.";
Schey K.L., Little M., Fowler J.G., Crouch R.K.;
Invest. Ophthalmol. Vis. Sci. 41:175-182(2000).
Cited for: PHOSPHORYLATION AT SER-235, DEAMIDATION AT ASN-246 AND ASN-259, ANDMASS SPECTROMETRY.
PubMed: 10634618