MIB2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MIB2_HUMAN
• UniProt AC: Q96AX9
• Protein Name: E3 ubiquitin-protein ligase MIB2
• Gene Name: MIB2
• Organism: Homo sapiens (Human).
• Sequence Length: 1013
• Subcellular Localization: Cytoplasm . Endosome . Colocalizes with endosomal compartments.
• Protein Description: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors..
• Protein Sequence: MGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1 (in isoform 10)	Acetylation	-	54.78	22814378
1 (in isoform 8)	Acetylation	-	54.78	22814378
14	Phosphorylation	EARGQSQSFQASGLQ HHCCCCCCCHHCCCC	25.27	29978859
18	Phosphorylation	QSQSFQASGLQPRSL CCCCCHHCCCCHHHH	29.29	29978859
24	Phosphorylation	ASGLQPRSLKAARRA HCCCCHHHHHHHHHH	40.69	29978859
61	Ubiquitination	AAPPNMDPDPQAGVQ CCCCCCCCCCCCCCE	44.40	27667366
79	Ubiquitination	RVVRGVDWKWGQQDG EEEECCCCCCCCCCC	8.90	23503661
80	Ubiquitination	VVRGVDWKWGQQDGG EEECCCCCCCCCCCC	36.66	-
109	Phosphorylation	SPSTPDRTVVVQWDQ CCCCCCCEEEEEECC	25.65	28192239
122	Phosphorylation	DQGTRTNYRAGYQGA CCCCCCCCCCCCCCC	10.96	28192239
137	Ubiquitination	HDLLLYDNAQIGVRH CCEEEECCCCCCCCC	21.86	-
162	Ubiquitination	HGLRGMRWKCRVCLD HCCCCCCEEEEEEEC	8.66	27667366
163	Ubiquitination	GLRGMRWKCRVCLDY CCCCCCEEEEEEECH	11.20	21890473
163 (in isoform 7)	Ubiquitination	-	11.20	21890473
176	Ubiquitination	DYDLCTQCYMHNKHE CHHHHCHHHHCCHHH	1.43	27667366
177	Ubiquitination	YDLCTQCYMHNKHEL HHHHCHHHHCCHHHH	7.50	29967540
180	Ubiquitination	CTQCYMHNKHELAHA HCHHHHCCHHHHHHH	30.74	23503661
186	Phosphorylation	HNKHELAHAFDRYET CCHHHHHHHHHHHHC	39.28	32645325
194	Ubiquitination	AFDRYETAHSRPVTL HHHHHHCCCCCCCCC	6.16	23503661
197	Ubiquitination	RYETAHSRPVTLSPR HHHCCCCCCCCCCCC	21.60	29967540
199	Ubiquitination	ETAHSRPVTLSPRQG HCCCCCCCCCCCCCC	9.04	21890473
199	Ubiquitination	ETAHSRPVTLSPRQG HCCCCCCCCCCCCCC	9.04	21890473
200	Phosphorylation	TAHSRPVTLSPRQGL CCCCCCCCCCCCCCC	24.97	22817900
202	Phosphorylation	HSRPVTLSPRQGLPR CCCCCCCCCCCCCCC	14.52	22817900
220	Ubiquitination	RGIFQGAKVVRGPDW CHHHCCCEEEECCCC	48.35	-
224	Ubiquitination	QGAKVVRGPDWEWGS CCCEEEECCCCCCCC	16.95	29967540
224 (in isoform 2)	Ubiquitination	-	16.95	-
238	Ubiquitination	SQDGGEGKPGRVVDI CCCCCCCCCCCEEEE	39.74	29967540
243	Ubiquitination	EGKPGRVVDIRGWDV CCCCCCEEEEECEEC	4.95	23503661
255	Phosphorylation	WDVETGRSVASVTWA EECCCCCEEEEEEEC	24.31	22461510
257	Phosphorylation	VETGRSVASVTWADG CCCCCEEEEEEECCC	10.26	17081983
257 (in isoform 4)	Ubiquitination	-	10.26	21890473
259	Phosphorylation	TGRSVASVTWADGTT CCCEEEEEEECCCCC	3.60	17081983
260	Phosphorylation	GRSVASVTWADGTTN CCEEEEEEECCCCCE	16.67	22461510
263	Ubiquitination	VASVTWADGTTNVYR EEEEEECCCCCEEEE	46.67	27667366
264	Ubiquitination	ASVTWADGTTNVYRV EEEEECCCCCEEEEC	27.05	21890473
264 (in isoform 8)	Ubiquitination	-	27.05	21890473
268	Ubiquitination	WADGTTNVYRVGHKG ECCCCCEEEECCCCC	2.79	21890473
268 (in isoform 7)	Ubiquitination	-	2.79	21890473
269	Phosphorylation	ADGTTNVYRVGHKGK CCCCCEEEECCCCCC	11.14	22461510
277	Ubiquitination	RVGHKGKVDLKCVGE ECCCCCCEEEEEEEE	16.56	27667366
278	Ubiquitination	VGHKGKVDLKCVGEA CCCCCCEEEEEEEEC	43.85	21890473
279	Ubiquitination	GHKGKVDLKCVGEAA CCCCCEEEEEEEECC	5.33	30230243
281	Ubiquitination	KGKVDLKCVGEAAGG CCCEEEEEEEECCCC	6.55	23503661
289	Ubiquitination	VGEAAGGFYYKDHLP EEECCCCEEECCCCC	6.19	21890473
292	Ubiquitination	AAGGFYYKDHLPRLG CCCCEEECCCCCCCC	27.56	29967540
295	Ubiquitination	GFYYKDHLPRLGKPA CEEECCCCCCCCCHH	3.66	23503661
301	Phosphorylation	HLPRLGKPAELQRRV CCCCCCCHHHHHHHH	29.21	32645325
304	Ubiquitination	RLGKPAELQRRVSAD CCCCHHHHHHHHCCC	5.36	21890473
304	Ubiquitination	RLGKPAELQRRVSAD CCCCHHHHHHHHCCC	5.36	21890473
308 (in isoform 2)	Ubiquitination	-	6.36	21890473
309	Phosphorylation	AELQRRVSADSQPFQ HHHHHHHCCCCCCCC	25.25	25159151
312	Phosphorylation	QRRVSADSQPFQHGD HHHHCCCCCCCCCCC	39.20	23927012
312	Ubiquitination	QRRVSADSQPFQHGD HHHHCCCCCCCCCCC	39.20	29967540
314	Ubiquitination	RVSADSQPFQHGDKV HHCCCCCCCCCCCEE	34.51	21890473
317	Phosphorylation	ADSQPFQHGDKVKCL CCCCCCCCCCEEEEE	46.96	-
320	Ubiquitination	QPFQHGDKVKCLLDT CCCCCCCEEEEEECH	48.46	-
322	Ubiquitination	FQHGDKVKCLLDTDV CCCCCEEEEEECHHH	25.55	21890473
322 (in isoform 3)	Ubiquitination	-	25.55	21890473
322 (in isoform 5)	Ubiquitination	-	25.55	21890473
322 (in isoform 6)	Ubiquitination	-	25.55	21890473
322 (in isoform 9)	Ubiquitination	-	25.55	21890473
326	Phosphorylation	DKVKCLLDTDVLREM CEEEEEECHHHHHHH	28.10	-
353	Phosphorylation	EFIGQTGTVHRITDR HHHCCCCCEEEECCC	19.27	28857561
357	Ubiquitination	QTGTVHRITDRGDVR CCCCEEEECCCCCEE	2.57	29967540
362 (in isoform 4)	Ubiquitination	-	26.41	21890473
365	Ubiquitination	TDRGDVRVQFNHETR CCCCCEEEEECCCCC	8.39	21890473
365	Ubiquitination	TDRGDVRVQFNHETR CCCCCEEEEECCCCC	8.39	21890473
365 (in isoform 1)	Ubiquitination	-	8.39	21890473
366 (in isoform 14)	Phosphorylation	-	23.90	24719451
366	Phosphorylation	DRGDVRVQFNHETRW CCCCEEEEECCCCCE	23.90	32645325
369	Phosphorylation	DVRVQFNHETRWTFH CEEEEECCCCCEEEC	38.81	-
369 (in isoform 8)	Ubiquitination	-	38.81	21890473
374	Phosphorylation	FNHETRWTFHPGALT ECCCCCEEECCCCCC	14.58	24719451
377	Ubiquitination	ETRWTFHPGALTKHH CCCEEECCCCCCCCC	26.09	-
379	Ubiquitination	RWTFHPGALTKHHSF CEEECCCCCCCCCCE	19.26	21890473
381	Phosphorylation	TFHPGALTKHHSFWV EECCCCCCCCCCEEC	28.00	24719451
388 (in isoform 2)	Ubiquitination	-	4.40	-
390	Ubiquitination	HHSFWVGDVVRVIGD CCCEECCHHEEEECC	26.56	21890473
391 (in isoform 6)	Ubiquitination	-	2.14	21890473
392 (in isoform 5)	Ubiquitination	-	4.87	21890473
394	Ubiquitination	WVGDVVRVIGDLDTV ECCHHEEEECCHHHH	3.76	30230243
402	Ubiquitination	IGDLDTVKRLQAGHG ECCHHHHHHHHCCCC	49.86	-
404	Ubiquitination	DLDTVKRLQAGHGEW CHHHHHHHHCCCCCC	3.20	21890473
412	Ubiquitination	QAGHGEWTDDMAPAL HCCCCCCCCCCHHHH	20.16	27667366
412	Phosphorylation	QAGHGEWTDDMAPAL HCCCCCCCCCCHHHH	20.16	29209046
413 (in isoform 2)	Ubiquitination	-	44.77	21890473
419	Ubiquitination	TDDMAPALGRVGKVV CCCCHHHHHCCEEEE	4.36	21890473
420	Ubiquitination	DDMAPALGRVGKVVK CCCHHHHHCCEEEEE	25.08	27667366
423	Ubiquitination	APALGRVGKVVKVFG HHHHHCCEEEEEEEC	18.71	23503661
423 (in isoform 3)	Ubiquitination	-	18.71	21890473
426	Ubiquitination	LGRVGKVVKVFGDGN HHCCEEEEEEECCCC	5.08	27667366
427	Ubiquitination	GRVGKVVKVFGDGNL HCCEEEEEEECCCCE	34.53	21890473
427 (in isoform 9)	Ubiquitination	-	34.53	21890473
430	Ubiquitination	GKVVKVFGDGNLRVA EEEEEEECCCCEEEE	44.92	23503661
431 (in isoform 14)	Phosphorylation	-	31.20	24719451
431	Phosphorylation	KVVKVFGDGNLRVAV EEEEEECCCCEEEEE	31.20	-
438 (in isoform 14)	Phosphorylation	-	5.86	24719451
438	Phosphorylation	DGNLRVAVAGQRWTF CCCEEEEEECCCEEE	5.86	-
438	Ubiquitination	DGNLRVAVAGQRWTF CCCEEEEEECCCEEE	5.86	23503661
444	Ubiquitination	AVAGQRWTFSPSCLV EEECCCEEECCCEEE	19.20	23503661
445	Ubiquitination	VAGQRWTFSPSCLVA EECCCEEECCCEEEE	8.85	23503661
448	Ubiquitination	QRWTFSPSCLVAYRP CCEEECCCEEEEECC	20.91	21890473
455	Ubiquitination	SCLVAYRPEEDANLD CEEEEECCHHHCCCC	36.33	23503661
459	Ubiquitination	AYRPEEDANLDVAER EECCHHHCCCCHHHH	23.05	23503661
463	Ubiquitination	EEDANLDVAERAREN HHHCCCCHHHHHHHC	7.21	21890473
466	Ubiquitination	ANLDVAERARENKSS CCCCHHHHHHHCHHH	29.25	23503661
470	Ubiquitination	VAERARENKSSLSVA HHHHHHHCHHHHHHH	43.91	21890473
470	Ubiquitination	VAERARENKSSLSVA HHHHHHHCHHHHHHH	43.91	21890473
470 (in isoform 1)	Ubiquitination	-	43.91	21890473
472	Phosphorylation	ERARENKSSLSVALD HHHHHCHHHHHHHHH	48.36	20068231
472	Ubiquitination	ERARENKSSLSVALD HHHHHCHHHHHHHHH	48.36	29967540
473	Phosphorylation	RARENKSSLSVALDK HHHHCHHHHHHHHHH	26.69	20068231
475	Phosphorylation	RENKSSLSVALDKLR HHCHHHHHHHHHHHH	13.80	20068231
476	Ubiquitination	ENKSSLSVALDKLRA HCHHHHHHHHHHHHH	8.12	23503661
480	Ubiquitination	SLSVALDKLRAQKSD HHHHHHHHHHHCCCC	40.50	21890473
484	Ubiquitination	ALDKLRAQKSDPEHP HHHHHHHCCCCCCCC	39.07	21890473
491	Ubiquitination	QKSDPEHPGRLVVEV CCCCCCCCCCEEEEE	28.12	21890473
501	Ubiquitination	LVVEVALGNAARALD EEEEEHHHHHHHHHH	16.40	21890473
505	Ubiquitination	VALGNAARALDLLRR EHHHHHHHHHHHHHH	33.02	21890473
514	Ubiquitination	LDLLRRRPEQVDTKN HHHHHHCHHHCCCCC	34.26	21890473
522	Ubiquitination	EQVDTKNQGRTALQV HHCCCCCCCHHHHHH	44.28	21890473
528	Ubiquitination	NQGRTALQVAAYLGQ CCCHHHHHHHHHHCH	21.97	21890473
529	Phosphorylation	QGRTALQVAAYLGQV CCHHHHHHHHHHCHH	3.24	20068231
530	Phosphorylation	GRTALQVAAYLGQVE CHHHHHHHHHHCHHH	4.21	20068231
537	Ubiquitination	AAYLGQVELIRLLLQ HHHHCHHHHHHHHHH	30.50	-
564	Ubiquitination	NTALHYAALGNQPEA CCHHHHHHHCCCCHH	14.61	23503661
566	Ubiquitination	ALHYAALGNQPEATR HHHHHHHCCCCHHHH	26.46	29967540
572	Ubiquitination	LGNQPEATRVLLSAG HCCCCHHHHHHHHCC	21.46	23503661
589	Ubiquitination	ADAINSTQSTALHVA HHHCCCCHHHHHHHH	38.31	21890473
597	Ubiquitination	STALHVAVQRGFLEV HHHHHHHHHHHHHHH	3.76	21890473
598	Ubiquitination	TALHVAVQRGFLEVV HHHHHHHHHHHHHHH	30.00	21890473
608	Ubiquitination	FLEVVRALCERGCDV HHHHHHHHHHCCCCC	1.87	23503661
615	Ubiquitination	LCERGCDVNLPDAHS HHHCCCCCCCCCCCC	10.35	23503661
623	Ubiquitination	NLPDAHSDTPLHSAI CCCCCCCCCCCCHHH	42.34	23503661
625	Ubiquitination	PDAHSDTPLHSAISA CCCCCCCCCCHHHHC	32.82	23503661
629	Ubiquitination	SDTPLHSAISAGTGA CCCCCCHHHHCCCCC	6.76	23503661
633	Ubiquitination	LHSAISAGTGASGIV CCHHHHCCCCCCCHH	20.21	21890473
640	Ubiquitination	GTGASGIVEVLTEVP CCCCCCHHHHHCCCC	4.80	21890473
648	Ubiquitination	EVLTEVPNIDVTATN HHHCCCCCCEEEEEC	48.55	21890473
650	Ubiquitination	LTEVPNIDVTATNSQ HCCCCCCEEEEECCC	38.05	21890473
654	Ubiquitination	PNIDVTATNSQGFTL CCCEEEEECCCCEEE	26.49	21890473
681	Ubiquitination	VRKILARARQLVDAK HHHHHHHHHHHHHHC	9.66	29967540
689	Ubiquitination	RQLVDAKKEDGFTAL HHHHHHCCCCCCCHH	63.86	-
716	Ubiquitination	QILIREGRCDVNVRN HHHHHCCCCCCEECC	14.54	27667366
719	Ubiquitination	IREGRCDVNVRNRKL HHCCCCCCEECCCCC	9.03	30230243
737	Ubiquitination	LHLAVQQAHVGLVPL HHHHHHHHCCCHHHE	5.13	27667366
746	Ubiquitination	VGLVPLLVDAGCSVN CCHHHEEEECCCCCC	6.63	-
834	Ubiquitination	RGRSPLDLAAEGRVL CCCCHHHHHHHHHHH	6.83	30230243
838	Ubiquitination	PLDLAAEGRVLKALQ HHHHHHHHHHHHHHH	21.92	27667366
842	Methylation	AAEGRVLKALQGCAQ HHHHHHHHHHHHHHH	44.16	115973001
842	Ubiquitination	AAEGRVLKALQGCAQ HHHHHHHHHHHHHHH	44.16	-
852	Ubiquitination	QGCAQRFRERQAGGG HHHHHHHHHHHCCCC	40.14	27667366
863	Ubiquitination	AGGGAAPGPRQTLGT CCCCCCCCCCCCCCC	25.87	27667366
870	Phosphorylation	GPRQTLGTPNTVTNL CCCCCCCCCCCEEEE	19.24	26853621
885	Ubiquitination	HVGAAPGPEAAECLV ECCCCCCHHHHHHHH	27.30	27667366
895	Ubiquitination	AECLVCSELALLVLF HHHHHHHHHHHHHHH	32.70	27667366
899	Methylation	VCSELALLVLFSPCQ HHHHHHHHHHHCCCC	2.33	-
899	Ubiquitination	VCSELALLVLFSPCQ HHHHHHHHHHHCCCC	2.33	27667366
930	Acetylation	RCQVVVSKKLRPDGS CCEEEEEECCCCCCC	44.19	25953088
939	Ubiquitination	LRPDGSEVASAAPAP CCCCCCCCCCCCCCC	5.66	27667366
949	Ubiquitination	AAPAPGPPRQLVEEL CCCCCCCHHHHHHHH	42.43	27667366
953	Ubiquitination	PGPPRQLVEELQSRY CCCHHHHHHHHHHHH	3.98	27667366
958	Phosphorylation	QLVEELQSRYRQMEE HHHHHHHHHHHHHHH	43.85	27762562
1012	Ubiquitination	IRDRIQIFV------ CHHCEEECC------	2.82	27667366
1013	Ubiquitination	RDRIQIFV------- HHCEEECC-------	9.07	27667366
1037	Ubiquitination	------------------------------- -------------------------------		27667366
1045	Ubiquitination	--------------------------------------- ---------------------------------------		27667366
1046	Ubiquitination	---------------------------------------- ----------------------------------------		27667366
1048	Ubiquitination	------------------------------------------ ------------------------------------------		27667366
1069	Ubiquitination	--------------------------------------------------------------- ---------------------------------------------------------------		27667366
1081	Ubiquitination	--------------------------------------------------------------------------- ---------------------------------------------------------------------------		27667366
1088	Ubiquitination	---------------------------------------------------------------------------------- ----------------------------------------------------------------------------------		27667366
1096	Ubiquitination	------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------		27667366
1098	Ubiquitination	-------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------		27667366
1102	Ubiquitination	------------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------------		27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MIB2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MIB2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MIB2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ACTS_HUMAN	ACTA1	physical	14507647
BCL10_MOUSE	Bcl10	physical	21896478
BCL10_HUMAN	BCL10	physical	21896478
M3K7_HUMAN	MAP3K7	physical	21896478
NEMO_HUMAN	IKBKG	physical	21896478
TBK1_HUMAN	TBK1	physical	21903422
UBE2N_HUMAN	UBE2N	physical	21903422
CYLD_HUMAN	CYLD	physical	25565632

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-200 AND SER-202, ANDMASS SPECTROMETRY.
PubMed: 17081983