MAGF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MAGF1_HUMAN
• UniProt AC: Q9HAY2
• Protein Name: Melanoma-associated antigen F1 {ECO:0000305}
• Gene Name: MAGEF1 {ECO:0000312|HGNC:HGNC:29639}
• Organism: Homo sapiens (Human).
• Sequence Length: 307
• Protein Description: Enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair. [PubMed: 29225034]
• Protein Sequence: MLQTPESRGLPVPQAEGEKDGGHDGETRAPTASQERPKEELGAGREEGAAEPALTRKGARALAAKALARRRAYRRLNRTVAELVQFLLVKDKKKSPITRSEMVKYVIGDLKILFPDIIARAAEHLRYVFGFELKQFDRKHHTYILINKLKPLEEEEEEDLGGDGPRLGLLMMILGLIYMRGNSAREAQVWEMLRRLGVQPSKYHFLFGYPKRLIMEDFVQQRYLSYRRVPHTNPPEYEFSWGPRSNLEISKMEVLGFVAKLHKKEPQHWPVQYREALADEADRARAKARAEASMRARASARAGIHLW

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MLQTPESRGLP ----CCCCCHHCCCC	25.68	26853621
38	Ubiquitination	TASQERPKEELGAGR CCCCCCCHHHHCCCC	70.78	22817900
65	Ubiquitination	GARALAAKALARRRA HHHHHHHHHHHHHHH	38.02	-
95	Phosphorylation	LVKDKKKSPITRSEM HCCCCCCCCCCHHHH	30.34	22167270
98	Phosphorylation	DKKKSPITRSEMVKY CCCCCCCCHHHHHHH	31.37	22167270
104	Ubiquitination	ITRSEMVKYVIGDLK CCHHHHHHHHHCCHH	32.27	22817900
148	Ubiquitination	HTYILINKLKPLEEE CEEEEEECCCCCCHH	51.39	22817900
150	Ubiquitination	YILINKLKPLEEEEE EEEEECCCCCCHHHH	49.52	22817900
251	Ubiquitination	RSNLEISKMEVLGFV CCCCEEHHHHHHHHH	44.75	-
252	Ubiquitination	SNLEISKMEVLGFVA CCCEEHHHHHHHHHH	3.25	21906983
264	Ubiquitination	FVAKLHKKEPQHWPV HHHHHHCCCCCCCCC	64.97	29967540
273	Phosphorylation	PQHWPVQYREALADE CCCCCCHHHHHHHHH	15.62	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MAGF1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MAGF1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MAGF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EID1_HUMAN	EID1	physical	21364888
EID2_HUMAN	EID2	physical	21364888
EID2B_HUMAN	EID2B	physical	21364888
NSE1_MOUSE	Nsmce1	physical	24911587
PIAS1_MOUSE	Pias1	physical	24911587
TRAIP_HUMAN	TRAIP	physical	25590999
PHF7_HUMAN	PHF7	physical	25590999

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.