M3K4_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: M3K4_MOUSE
• UniProt AC: O08648
• Protein Name: Mitogen-activated protein kinase kinase kinase 4
• Gene Name: Map3k4
• Organism: Mus musculus (Mouse).
• Sequence Length: 1597
• Subcellular Localization: Cytoplasm, perinuclear region . Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.
• Protein Description: Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6..
• Protein Sequence: MRDAIAEPVPPPALADTPAAAMEELRPAPPPQPEPDPECCPAARQECMLGESARKSMESDPEDFSDETNTETLYGTSPPSTPRQMKRLSAKHQRNSAGRPASRSNLKEKMNTPSQSPHKDLGKGVETVEEYSYKQEKKIRATLRTTERDHKKNAQCSFMLDSVAGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSMPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTAAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYGSIAFSSNGAGFNGPLVEGQCRTPQETNRVGCSSYHEHLQRQRVSFEQVKRIMELLEYMEALYPSLQALQKDYERYAAKDFEDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGYEPEDEVEDTEVELRELESGTEESDEEPTPSPRVPELRLSTDAILDSRSQGCVSRKLERLESEEDSIGWGTADCGPEASRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMNGSLQRARVALVKDDRPVEFSDFPGPMWGSDYVQLSGTPPSSEQKCSAVSWEELRAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLGGLEKTDCNMDAFEEDLQKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLESGLQESCAELWTSADDNGAADELRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFVLSASARELLDALKAKQYVKVQIPGLENLHVFVPDSLAEEKKIILQLLNAATGKDCSKDPDDVFMDAFLLLTKHGDRARDSEDGWGTWEARAVKIVPQVETVDTLRSMQVDNLLLVVMESAHLVLQRKAFQQSIEGLMTVRHEQTSSQPIIAKGLQQLKNDALELCNRISDAIDRVDHMFTLEFDAEVEESESATLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFAQKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARTHGNSVAAAAAVAAAATTAAGRPGPGGGDSVPAKPVNTAPDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRSDSSGSTRRSWELRTLISQTKDSASKQGPIEAIQKSVRLFEERRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYTKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKAFLSHCLESDPKIRWTASQLLDHAFVKVCTDEE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
56	Phosphorylation	LGESARKSMESDPED HCHHHHHHCCCCCCC	23.41	21149613
59	Phosphorylation	SARKSMESDPEDFSD HHHHHCCCCCCCCCC	50.23	21149613
65	Phosphorylation	ESDPEDFSDETNTET CCCCCCCCCCCCCCE	47.03	21149613
68	Phosphorylation	PEDFSDETNTETLYG CCCCCCCCCCCEECC	53.05	25293948
70	Phosphorylation	DFSDETNTETLYGTS CCCCCCCCCEECCCC	37.45	21149613
72	Phosphorylation	SDETNTETLYGTSPP CCCCCCCEECCCCCC	24.20	21149613
74	Phosphorylation	ETNTETLYGTSPPST CCCCCEECCCCCCCC	26.27	25293948
76	Phosphorylation	NTETLYGTSPPSTPR CCCEECCCCCCCCHH	25.22	21149613
77	Phosphorylation	TETLYGTSPPSTPRQ CCEECCCCCCCCHHH	30.37	21149613
80	Phosphorylation	LYGTSPPSTPRQMKR ECCCCCCCCHHHHHH	55.20	25293948
81	Phosphorylation	YGTSPPSTPRQMKRL CCCCCCCCHHHHHHH	28.41	21149613
112	Phosphorylation	NLKEKMNTPSQSPHK HHHHHHCCCCCCCCC	22.33	21659605
114	Phosphorylation	KEKMNTPSQSPHKDL HHHHCCCCCCCCCCC	41.05	25263469
116	Phosphorylation	KMNTPSQSPHKDLGK HHCCCCCCCCCCCCC	33.05	26824392
171	Phosphorylation	AGSLPKKSIPDVDLN CCCCCCCCCCCCCCC	45.54	23984901
194	Phosphorylation	SNAKLPVSMPMPIAR CCCCCCCCCCCCCCC	18.41	26525534
206	Phosphorylation	IARTARQTSRTDCPA CCCCCCHHCCCCCCH	18.02	23140645
207	Phosphorylation	ARTARQTSRTDCPAD CCCCCHHCCCCCCHH	25.50	27087446
209	Phosphorylation	TARQTSRTDCPADRL CCCHHCCCCCCHHHH	41.54	25367039
232	Phosphorylation	LLKLTSVSKKKDREQ HHHHHHHCCCCHHHH	38.23	20139300
343	Phosphorylation	HLQRQRVSFEQVKRI HHHHCCCCHHHHHHH	25.84	29899451
356	Phosphorylation	RIMELLEYMEALYPS HHHHHHHHHHHHHHH	10.54	27357545
361	Phosphorylation	LEYMEALYPSLQALQ HHHHHHHHHHHHHHH	9.60	27357545
363	Phosphorylation	YMEALYPSLQALQKD HHHHHHHHHHHHHHH	21.40	27357545
424	Phosphorylation	WPVFEIPSPRPSKGY CCEEECCCCCCCCCC	38.92	26824392
428	Phosphorylation	EIPSPRPSKGYEPED ECCCCCCCCCCCCHH	39.74	22942356
431	Phosphorylation	SPRPSKGYEPEDEVE CCCCCCCCCCHHHCC	31.86	30635358
440	Phosphorylation	PEDEVEDTEVELREL CHHHCCCCEEEEEHH	28.55	30635358
449	Phosphorylation	VELRELESGTEESDE EEEEHHHCCCCCCCC	64.74	25521595
451	Phosphorylation	LRELESGTEESDEEP EEHHHCCCCCCCCCC	45.95	21082442
454	Phosphorylation	LESGTEESDEEPTPS HHCCCCCCCCCCCCC	44.05	25521595
459	Phosphorylation	EESDEEPTPSPRVPE CCCCCCCCCCCCCCC	39.41	25619855
461	Phosphorylation	SDEEPTPSPRVPELR CCCCCCCCCCCCCCC	28.02	25619855
477	Phosphorylation	STDAILDSRSQGCVS CCHHHHCCCCCCHHH	30.31	-
492	Phosphorylation	RKLERLESEEDSIGW HHHHHHHCCCCCCCC	51.37	25521595
496	Phosphorylation	RLESEEDSIGWGTAD HHHCCCCCCCCCCCC	26.70	25619855
638	Phosphorylation	AGEPSLLSIKQLVRE CCCCCHHHHHHHHHH	32.79	26026062
674	Phosphorylation	VLGGLEKTDCNMDAF HHCCHHHCCCCCHHH	36.09	-
968	Phosphorylation	MTVRHEQTSSQPIIA HHCCCCCCCCCCCHH	27.62	28418008
969	Phosphorylation	TVRHEQTSSQPIIAK HCCCCCCCCCCCHHH	27.03	28418008
970	Phosphorylation	VRHEQTSSQPIIAKG CCCCCCCCCCCHHHH	43.28	25266776
1126	Phosphorylation	HVIGKPHSPVTAIHR HHHCCCCCCCEEECC	29.59	26824392
1129	Phosphorylation	GKPHSPVTAIHRNSP CCCCCCCEEECCCCC	24.23	24759943
1135	Phosphorylation	VTAIHRNSPRPVKVP CEEECCCCCCCCCCC	23.42	22817900
1146 (in isoform 2)	Phosphorylation	-	56.27	22324799
1146	Phosphorylation	VKVPRCHSDPPNPHL CCCCCCCCCCCCCCE	56.27	25266776
1176	Phosphorylation	DARTHGNSVAAAAAV CHHHCCHHHHHHHHH	20.09	22817900
1188	Phosphorylation	AAVAAAATTAAGRPG HHHHHHHHHHCCCCC	16.55	-
1189	Phosphorylation	AVAAAATTAAGRPGP HHHHHHHHHCCCCCC	15.13	-
1217	Phosphorylation	APDTRGSSVPENDRL CCCCCCCCCCCCCHH	44.51	26824392
1235	Phosphorylation	AAELQFRSLSRHSSP HHHHHHHHHHCCCCC	31.80	26643407
1237	Phosphorylation	ELQFRSLSRHSSPTE HHHHHHHHCCCCCCC	29.24	25266776
1240	Phosphorylation	FRSLSRHSSPTEERD HHHHHCCCCCCCCCC	36.58	20469934
1241	Phosphorylation	RSLSRHSSPTEERDE HHHHCCCCCCCCCCC	29.71	26824392
1243	Phosphorylation	LSRHSSPTEERDEPA HHCCCCCCCCCCCCC	53.04	23684622
1256	Phosphorylation	PAYPRSDSSGSTRRS CCCCCCCCCCCCHHH	37.53	23984901
1257	Phosphorylation	AYPRSDSSGSTRRSW CCCCCCCCCCCHHHH	41.58	25266776
1259	Phosphorylation	PRSDSSGSTRRSWEL CCCCCCCCCHHHHHH	22.23	26643407
1260	Phosphorylation	RSDSSGSTRRSWELR CCCCCCCCHHHHHHH	33.36	23984901
1263	Phosphorylation	SSGSTRRSWELRTLI CCCCCHHHHHHHHHH	22.93	26643407
1268	Phosphorylation	RRSWELRTLISQTKD HHHHHHHHHHHHCCC	40.86	25777480
1271	Phosphorylation	WELRTLISQTKDSAS HHHHHHHHHCCCCHH	34.60	25777480
1483	Phosphorylation	KLKNNAQTMPGEVNS EEECCCCCCCCCCHH	23.95	25338131
1491	Phosphorylation	MPGEVNSTLGTAAYM CCCCCHHHHHHHHHH	24.81	25338131

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of M3K4_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of M3K4_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of M3K4_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AXIN1_MOUSE	Axin1	physical	12878610
TRAF4_MOUSE	Traf4	physical	16157600
M3K4_MOUSE	Map3k4	physical	17726008
AXIN1_MOUSE	Axin1	physical	18316368

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492, AND MASSSPECTROMETRY.
PubMed: 17242355