M2K3_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: M2K3_ARATH
• UniProt AC: O80396
• Protein Name: Mitogen-activated protein kinase kinase 3
• Gene Name: MKK3
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 520
• Protein Description: MKK3-MPK6 module plays an important role in the jasmonate signal transduction pathway through the negative regulation of MYC2/JIN1 expression. Activates by phosphorylation the downstream MPK6, MPK7 and MPK8. MKK3-MPK7 module acts as a positive regulator of PR1 gene expression. MKK3-MPK8 module negatively regulates ROS accumulation through controlling expression of the RBOHD gene. Component of the abscisic acid (ABA) signaling pathway that may act as ABA signal transducer in the context of abiotic stresses. Activator of the C group MAP kinases. Activates MPK7 in response to ABA. [PubMed: 25720833]
• Protein Sequence: MAALEELKKKLSPLFDAEKGFSSSSSLDPNDSYLLSDGGTVNLLSRSYGVYNFNELGLQKCTSSHVDESESSETTYQCASHEMRVFGAIGSGASSVVQRAIHIPNHRILALKKINIFEREKRQQLLTEIRTLCEAPCHEGLVDFHGAFYSPDSGQISIALEYMNGGSLADILKVTKKIPEPVLSSLFHKLLQGLSYLHGVRHLVHRDIKPANLLINLKGEPKITDFGISAGLENSMAMCATFVGTVTYMSPERIRNDSYSYPADIWSLGLALFECGTGEFPYIANEGPVNLMLQILDDPSPTPPKQEFSPEFCSFIDACLQKDPDARPTADQLLSHPFITKHEKERVDLATFVQSIFDPTQRLKDLADMLTIHYYSLFDGFDDLWHHAKSLYTETSVFSFSGKHNTGSTEIFSALSDIRNTLTGDLPSEKLVHVVEKLHCKPCGSGGVIIRAVGSFIVGNQFLICGDGVQAEGLPSFKDLGFDVASRRVGRFQEQFVVESGDLIGKYFLAKQELYITNLD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
47	Phosphorylation	TVNLLSRSYGVYNFN CEEEEECCCCCCCHH		23572148
69	Phosphorylation	TSSHVDESESSETTY CCCCCCCCCCCCCHH		-
235	Phosphorylation	ISAGLENSMAMCATF CCCCCCCHHHHHHHH		17369371
241	Phosphorylation	NSMAMCATFVGTVTY CHHHHHHHHEEEEEE		17369371
245	Phosphorylation	MCATFVGTVTYMSPE HHHHHEEEEEECCHH		-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of M2K3_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
235	S	Phosphorylation	Phosphorylation at Ser-235 and Thr-241 by MAP kinase kinase kinases positively regulates kinase activity (Probable).	17369371
241	T	Phosphorylation	Phosphorylation at Ser-235 and Thr-241 by MAP kinase kinase kinases positively regulates kinase activity (Probable).	17369371

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of M2K3_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MPK1_ARATH	MPK1	physical	25680457
MPK2_ARATH	MPK2	physical	25680457
MPK7_ARATH	MPK7	physical	25680457
MYC2_ARATH	MYC2	genetic	25139007

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.