dbPTM

HSPB1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HSPB1_MOUSE
UniProt AC	P14602
Protein Name	Heat shock protein beta-1
Gene Name	Hspb1
Organism	Mus musculus (Mouse).
Sequence Length	209
Subcellular Localization	Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles o
Protein Description	Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. [PubMed: 17661394 Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (By similarity]
Protein Sequence	MTERRVPFSLLRSPSWEPFRDWYPAHSRLFDQAFGVPRLPDEWSQWFSAAGWPGYVRPLPAATAEGPAAVTLAAPAFSRALNRQLSSGVSEIRQTADRWRVSLDVNHFAPEELTVKTKEGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTLVSSSLSPEGTLTVEAPLPKAVTQSAEITIPVTFEARAQIGGPEAGKSEQSGAK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	TERRVPFSLLRSPSW CCCCCCHHHCCCCCC	22.03	24719451
12	Methylation	RVPFSLLRSPSWEPF CCCHHHCCCCCCCCC	52.38	24129315
13	Phosphorylation	VPFSLLRSPSWEPFR CCHHHCCCCCCCCCC	24.21	26824392
15	Phosphorylation	FSLLRSPSWEPFRDW HHHCCCCCCCCCCCC	45.37	1860870
27	Phosphorylation	RDWYPAHSRLFDQAF CCCCHHHHHHHHHHH	32.55	-
71	Phosphorylation	AEGPAAVTLAAPAFS CCCCEEEEEHHHHHH	13.12	-
78	Phosphorylation	TLAAPAFSRALNRQL EEHHHHHHHHHHHHH	20.81	-
86	Phosphorylation	RALNRQLSSGVSEIR HHHHHHHHHCHHHHH	19.39	1860870
87	Phosphorylation	ALNRQLSSGVSEIRQ HHHHHHHHCHHHHHH	52.37	24925903
90	Phosphorylation	RQLSSGVSEIRQTAD HHHHHCHHHHHHHHH	30.72	24925903
102	Phosphorylation	TADRWRVSLDVNHFA HHHHEEEEEECCCCC	15.41	26824392
116	Ubiquitination	APEELTVKTKEGVVE CCHHCEEEEECCEEE	48.95	22790023
118	Ubiquitination	EELTVKTKEGVVEIT HHCEEEEECCEEEEC	46.91	22790023
127	Acetylation	GVVEITGKHEERQDE CEEEECCCCCCCCCC	38.91	-
137	Phosphorylation	ERQDEHGYISRCFTR CCCCCCCCEEEEEEC	9.53	28464351
139	Phosphorylation	QDEHGYISRCFTRKY CCCCCCEEEEEECCC	17.95	-
144 (in isoform 3)	O-linked_Glycosylation	-	15.10	30016717
145	Ubiquitination	ISRCFTRKYTLPPGV EEEEEECCCCCCCCC	39.26	22790023
146	Phosphorylation	SRCFTRKYTLPPGVD EEEEECCCCCCCCCC	15.37	25293948
147	Phosphorylation	RCFTRKYTLPPGVDP EEEECCCCCCCCCCC	35.50	22006019
155	Phosphorylation	LPPGVDPTLVSSSLS CCCCCCCCEECCCCC	35.02	25293948
158	Phosphorylation	GVDPTLVSSSLSPEG CCCCCEECCCCCCCC	19.81	25293948
159	Phosphorylation	VDPTLVSSSLSPEGT CCCCEECCCCCCCCC	28.02	25293948
160	Phosphorylation	DPTLVSSSLSPEGTL CCCEECCCCCCCCCE	25.92	25293948
162	Phosphorylation	TLVSSSLSPEGTLTV CEECCCCCCCCCEEE	23.89	26824392
178	Phosphorylation	APLPKAVTQSAEITI ECCCCCCCCCEEEEE	22.97	26824392
180	Phosphorylation	LPKAVTQSAEITIPV CCCCCCCCEEEEEEE	20.64	26824392
188	O-linked_Glycosylation	AEITIPVTFEARAQI EEEEEEEEEEEEEEC	15.48	30016717
202	Ubiquitination	IGGPEAGKSEQSGAK CCCCCCCCCCCCCCC	58.84	22790023
203	Phosphorylation	GGPEAGKSEQSGAK- CCCCCCCCCCCCCC-	39.69	26824392
206	Phosphorylation	EAGKSEQSGAK---- CCCCCCCCCCC----	36.34	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
15	S	Phosphorylation	Kinase	MAPKAPK2	P49138	Uniprot
15	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
15	S	Phosphorylation	Kinase	PKA	-	Uniprot
15	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
15	S	Phosphorylation	Kinase	PKC	-	Uniprot
15	S	Phosphorylation	Kinase	PRKACA	P05132	GPS
15	S	Phosphorylation	Kinase	MAPKAPK3	Q3UMW7	Uniprot
15	S	Phosphorylation	Kinase	MAPKAPK2	P49137	PSP
15	S	Phosphorylation	Kinase	PRKCD	P28867	GPS
15	S	Phosphorylation	Kinase	PRKCA	P20444	GPS
15	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
15	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
86	S	Phosphorylation	Kinase	MAPKAPK5	O54992	Uniprot
86	S	Phosphorylation	Kinase	PKC	-	Uniprot
86	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
86	S	Phosphorylation	Kinase	PKA	-	Uniprot
86	S	Phosphorylation	Kinase	PKC_GROUP	-	PhosphoELM
86	S	Phosphorylation	Kinase	PKC-FAMILY	-	GPS
86	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
86	S	Phosphorylation	Kinase	MAPKAPK3	Q3UMW7	Uniprot
86	S	Phosphorylation	Kinase	MAPKAPK2	P49138	Uniprot
86	S	Phosphorylation	Kinase	MAPKAPK2	P49137	PSP
86	S	Phosphorylation	Kinase	PRKCD	P28867	GPS
86	S	Phosphorylation	Kinase	PRKCA	P20444	GPS
86	S	Phosphorylation	Kinase	PRKACA	P05132	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of HSPB1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HSPB1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KPCD_MOUSE	Prkcd	physical	15731106
HSPB1_MOUSE	Hspb1	physical	10383393
CISY_HUMAN	CS	physical	10383393
INS_HUMAN	INS	physical	10383393
CISY_PIG	CS	physical	8093612
AMYG_YEAST	SGA1	physical	8093612

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HSPB1_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, AND MASSSPECTROMETRY.	17242355 [Abstract]
"Identification of MAPKAP kinase 2 as a major enzyme responsible forthe phosphorylation of the small mammalian heat shock proteins."; Stokoe D., Engel K., Campbell D.G., Cohen P., Gaestel M.; FEBS Lett. 313:307-313(1992). Cited for: PHOSPHORYLATION AT SER-15 AND SER-86 BY MAPKAPK2.	1332886 [Abstract]
"Identification of the phosphorylation sites of the murine small heatshock protein hsp25."; Gaestel M., Schroeder W., Benndorf R., Lippmann C., Buchner K.,Hucho F., Erdmann V.A., Bielka H.; J. Biol. Chem. 266:14721-14724(1991). Cited for: PHOSPHORYLATION AT SER-15 AND SER-86.	1860870 [Abstract]