HAND1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: HAND1_MOUSE
• UniProt AC: Q64279
• Protein Name: Heart- and neural crest derivatives-expressed protein 1
• Gene Name: Hand1
• Organism: Mus musculus (Mouse).
• Sequence Length: 216
• Subcellular Localization: Nucleus, nucleoplasm . Nucleus, nucleolus . Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading
• Protein Description: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box)..
• Protein Sequence: MNLVGSYAHHHHHHHSHPPHPMLHEPFLFGPASRCHQERPYFQSWLLSPADAAPDFPAGGPPPTTAVAAAAYGPDARPSQSPGRLEALGSRLPKRKGSGPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDVLAKDAQAGDPEAFKAELKKTDGGRESKRKRELPQQPESFPPASGPGEKRIKGRTGWPQQVWALELNQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
107	Phosphorylation	PKKERRRTESINSAF CHHHHHHHHHHHHHH	32.07	22817900
109	Phosphorylation	KERRRTESINSAFAE HHHHHHHHHHHHHHH	26.85	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
107	T	Phosphorylation	Kinase	PLK4	O00444	PSP
107	T	Phosphorylation	Kinase	PLK4	Q64702	Uniprot
109	S	Phosphorylation	Kinase	PLK4	O00444	PSP
109	S	Phosphorylation	Kinase	PLK4	Q64702	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of HAND1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of HAND1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HTF4_MOUSE	Tcf12	physical	20211142
FHL2_MOUSE	Fhl2	physical	15509787
SOX15_MOUSE	Sox15	physical	16759287
NKX25_MOUSE	Nkx2-5	physical	12392994

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Nucleolar release of Hand1 acts as a molecular switch to determinecell fate.";
Martindill D.M.J., Risebro C.A., Smart N., Franco-Viseras Mdel M.,Rosario C.O., Swallow C.J., Dennis J.W., Riley P.R.;
Nat. Cell Biol. 9:1131-1141(2007).
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MDFIC, PHOSPHORYLATION ATTHR-107 AND SER-109, AND MUTAGENESIS OF THR-107 AND SER-109.
PubMed: 17891141