GAST_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: GAST_HUMAN
• UniProt AC: P01350
• Protein Name: Gastrin
• Gene Name: GAST
• Organism: Homo sapiens (Human).
• Sequence Length: 101
• Subcellular Localization: Secreted.
• Protein Description: Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine..
• Protein Sequence: MQRLCVYVLIFALALAAFSEASWKPRSQQPDAPLGTGANRDLELPWLEQQGPASHHRRQLGPQGPPHLVADPSKKQGPWLEEEEEAYGWMDFGRRSAEDEN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
22	Phosphorylation	LAAFSEASWKPRSQQ HHHHHHHCCCCHHHC	31.42	24719451
59	Pyrrolidone_carboxylic_acid	PASHHRRQLGPQGPP CCCHHHHHHCCCCCC	51.21	-
59	Pyrrolidone_carboxylic_acid	PASHHRRQLGPQGPP CCCHHHHHHCCCCCC	51.21	2730647
59	Pyrrolidone_carboxylic_acid	PASHHRRQLGPQGPP CCCHHHHHHCCCCCC	51.21	2730647
76	Pyrrolidone_carboxylic_acid	VADPSKKQGPWLEEE CCCCCCCCCCCHHHH	67.90	-
76	Pyrrolidone_carboxylic_acid	VADPSKKQGPWLEEE CCCCCCCCCCCHHHH	67.90	5921183
76	Pyrrolidone_carboxylic_acid	VADPSKKQGPWLEEE CCCCCCCCCCCHHHH	67.90	5921183
87	Phosphorylation	LEEEEEAYGWMDFGR HHHHHHHHCCHHCCC	18.01	8578591
87	Sulfation	LEEEEEAYGWMDFGR HHHHHHHHCCHHCCC	18.01	-
87	Sulfation	LEEEEEAYGWMDFGR HHHHHHHHCCHHCCC	18.01	7530658
92	Phenylalanine amide	EAYGWMDFGRRSAED HHHCCHHCCCCCCCC	4.79	-
92	Amidation	EAYGWMDFGRRSAED HHHCCHHCCCCCCCC	4.79	5921183
96	Phosphorylation	WMDFGRRSAEDEN-- CHHCCCCCCCCCC--	34.35	9797370

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
87	Y	Phosphorylation	Kinase	SRC	P12931	GPS
87	Y	Phosphorylation	Kinase	SRC64	-	PhosphoELM

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of GAST_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of GAST_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
XAGE3_HUMAN	XAGE3	physical	28514442
ATE1_HUMAN	ATE1	physical	28514442
ZZEF1_HUMAN	ZZEF1	physical	28514442
ZER1_HUMAN	ZER1	physical	28514442
HECD3_HUMAN	HECTD3	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Pyrrolidone carboxylic acid

"Structures of human gastrins I and II.";
Bentley P.H., Kenner G.W., Sheppard R.C.;
Nature 209:583-585(1966).
Cited for: PROTEIN SEQUENCE OF 76-92.
PubMed: 5921183

Sulfation

"Metabolism and acid secretory effect of sulfated and nonsulfatedgastrin-6 in humans.";
Palnaes Hansen C., Stadil F., Rehfeld J.F.;
Am. J. Physiol. 279:G903-G909(2000).
Cited for: PROTEOLYTIC PROCESSING, AND SULFATION AT TYR-87.
PubMed: 11052986

"Post-poly(Glu) cleavage and degradation modified by O-sulfatedtyrosine: a novel post-translational processing mechanism.";
Rehfeld J.F., Hansen C.P., Johnsen A.H.;
EMBO J. 14:389-396(1995).
Cited for: PROTEOLYTIC PROCESSING, MASS SPECTROMETRY, AND SULFATION AT TYR-87.
PubMed: 7530658