EVPL_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: EVPL_HUMAN
• UniProt AC: Q92817
• Protein Name: Envoplakin
• Gene Name: EVPL
• Organism: Homo sapiens (Human).
• Sequence Length: 2033
• Subcellular Localization: Cell junction, desmosome. Cornified envelope. Cytoplasm, cytoskeleton. Colocalized with DSP at desmosomes and along intermediate filaments.
• Protein Description: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments..
• Protein Sequence: MFKGLSKGSQGKGSPKGSPAKGSPKGSPSRHSRAATQELALLISRMQANADQVERDILETQKRLQQDRLNSEQSQALQHQQETGRSLKEAEVLLKDLFLDVDKARRLKHPQAEEIEKDIKQLHERVTQECAEYRALYEKMVLPPDVGPRVDWARVLEQKQKQVCAGQYGPGMAELEQQIAEHNILQKEIDAYGQQLRSLVGPDAATIRSQYRDLLKAASWRGQSLGSLYTHLQGCTRQLSALAEQQRRILQQDWSDLMADPAGVRREYEHFKQHELLSQEQSVNQLEDDGERMVELRHPAVGPIQAHQEALKMEWQNFLNLCICQETQLQHVEDYRRFQEEADSVSQTLAKLNSNLDAKYSPAPGGPPGAPTELLQQLEAEEKRLAVTERATGDLQRRSRDVAPLPQRRNPPQQPLHVDSICDWDSGEVQLLQGERYKLVDNTDPHAWVVQGPGGETKRAPAACFCIPAPDPDAVARASRLASELQALKQKLATVQSRLKASAVESLRPSQQAPSGSDLANPQAQKLLTQMTRLDGDLGQIERQVLAWARAPLSRPTPLEDLEGRIHSHEGTAQRLQSLGTEKETAQKECEAFLSTRPVGPAALQLPVALNSVKNKFSDVQVLCSLYGEKAKAALDLERQIQDADRVIRGFEATLVQEAPIPAEPGALQERVSELQRQRRELLEQQTCVLRLHRALKASEHACAALQNNFQEFCQDLPRQQRQVRALTDRYHAVGDQLDLREKVVQDAALTYQQFKNCKDNLSSWLEHLPRSQVRPSDGPSQIAYKLQAQKRLTQEIQSRERDRATASHLSQALQAALQDYELQADTYRCSLEPTLAVSAPKRPRVAPLQESIQAQEKNLAKAYTEVAAAQQQLLQQLEFARKMLEKKELSEDIRRTHDAKQGSESPAQAGRESEALKAQLEEERKRVARVQHELEAQRSQLLQLRTQRPLERLEEKEVVEFYRDPQLEGSLSRVKAQVEEEGKRRAGLQADLEVAAQKVVQLESKRKTMQPHLLTKEVTQVERDPGLDSQAAQLRIQIQQLRGEDAVISARLEGLKKELLALEKREVDVKEKVVVKEVVKVEKNLEMVKAAQALRLQMEEDAARRKQAEEAVAKLQARIEDLERAISSVEPKVIVKEVKKVEQDPGLLQESSRLRSLLEEERTKNATLARELSDLHSKYSVVEKQRPKVQLQERVHEIFQVDPETEQEITRLKAKLQEMAGKRSGVEKEVEKLLPDLEVLRAQKPTVEYKEVTQEVVRHERSPEVLREIDRLKAQLNELVNSHGRSQEQLIRLQGERDEWRRERAKVETKTVSKEVVRHEKDPVLEKEAERLRQEVREAAQKRRAAEDAVYELQSKRLLLERRKPEEKVVVQEVVVTQKDPKLREEHSRLSGSLDEEVGRRRQLELEVQQLRAGVEEQEGLLSFQEDRSKKLAVERELRQLTLRIQELEKRPPTVQEKIIMEEVVKLEKDPDLEKSTEALRWDLDQEKTQVTELNRECKNLQVQIDVLQKAKSQEKTIYKEVIRVQKDRVLEDERARVWEMLNRERTARQAREEEARRLRERIDRAETLGRTWSREESELQRARDQADQECGRLQQELRALERQKQQQTLQLQEESKLLSQKTESERQKAAQRGQELSRLEAAILREKDQIYEKERTLRDLHAKVSREELSQETQTRETNLSTKISILEPETGKDMSPYEAYKRGIIDRGQYLQLQELECDWEEVTTSGPCGEESVLLDRKSGKQYSIEAALRCRRISKEEYHLYKDGHLPISEFALLVAGETKPSSSLSIGSIISKSPLASPAPQSTSFFSPSFSLGLGDDSFPIAGIYDTTTDNKCSIKTAVAKNMLDPITGQKLLEAQAATGGIVDLLSRERYSVHKAMERGLIENTSTQRLLNAQKAFTGIEDPVTKKRLSVGEAVQKGWMPRESVLPHLQVQHLTGGLIDPKRTGRIPIQQALLSGMISEELAQLLQDESSYEKDLTDPISKERLSYKEAMGRCRKDPLSGLLLLPAALEGYRCYRSASPTVPRSLR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Methylation	-----MFKGLSKGSQ -----CCCCCCCCCC	71.45	-
6	Phosphorylation	--MFKGLSKGSQGKG --CCCCCCCCCCCCC	43.57	28348404
9	Phosphorylation	FKGLSKGSQGKGSPK CCCCCCCCCCCCCCC	39.22	24719451
18	Phosphorylation	GKGSPKGSPAKGSPK CCCCCCCCCCCCCCC	28.01	25849741
23	Phosphorylation	KGSPAKGSPKGSPSR CCCCCCCCCCCCCCH	23.80	25849741
27	O-linked_Glycosylation	AKGSPKGSPSRHSRA CCCCCCCCCCHHHHH	26.31	31492838
27	Phosphorylation	AKGSPKGSPSRHSRA CCCCCCCCCCHHHHH	26.31	25849741
29	O-linked_Glycosylation	GSPKGSPSRHSRAAT CCCCCCCCHHHHHHH	44.30	31492838
29	Phosphorylation	GSPKGSPSRHSRAAT CCCCCCCCHHHHHHH	44.30	20873877
88	Ubiquitination	QETGRSLKEAEVLLK HHHCCCHHHHHHHHH	57.00	-
95	Ubiquitination	KEAEVLLKDLFLDVD HHHHHHHHHHCCCHH	48.50	-
103	Ubiquitination	DLFLDVDKARRLKHP HHCCCHHHHHHCCCC	43.57	-
133	Phosphorylation	VTQECAEYRALYEKM HHHHHHHHHHHHHHC	5.22	-
192	Phosphorylation	LQKEIDAYGQQLRSL HHHHHHHHHHHHHHH	16.24	28152594
198	Phosphorylation	AYGQQLRSLVGPDAA HHHHHHHHHHCCCHH	35.68	21406692
206	Phosphorylation	LVGPDAATIRSQYRD HHCCCHHHHHHHHHH	20.74	21406692
209	Phosphorylation	PDAATIRSQYRDLLK CCHHHHHHHHHHHHH	27.93	19369195
219	Phosphorylation	RDLLKAASWRGQSLG HHHHHHHHHCCCCHH	23.36	20068231
224	Phosphorylation	AASWRGQSLGSLYTH HHHHCCCCHHHHHHH	37.21	20068231
227	Phosphorylation	WRGQSLGSLYTHLQG HCCCCHHHHHHHHHH	24.40	20068231
229	Phosphorylation	GQSLGSLYTHLQGCT CCCHHHHHHHHHHHH	8.20	19053533
230	Phosphorylation	QSLGSLYTHLQGCTR CCHHHHHHHHHHHHH	22.77	20068231
236	Phosphorylation	YTHLQGCTRQLSALA HHHHHHHHHHHHHHH	28.42	20068231
240	Phosphorylation	QGCTRQLSALAEQQR HHHHHHHHHHHHHHH	16.95	19053533
346	Phosphorylation	QEEADSVSQTLAKLN HHHHHHHHHHHHHHH	22.77	24719451
348	Phosphorylation	EADSVSQTLAKLNSN HHHHHHHHHHHHHHC	22.60	-
354	Phosphorylation	QTLAKLNSNLDAKYS HHHHHHHHCCCCCCC	49.56	-
360	Phosphorylation	NSNLDAKYSPAPGGP HHCCCCCCCCCCCCC	22.93	30278072
361	Phosphorylation	SNLDAKYSPAPGGPP HCCCCCCCCCCCCCC	17.55	30278072
372	Phosphorylation	GGPPGAPTELLQQLE CCCCCCCHHHHHHHH	38.44	20068231
383	Ubiquitination	QQLEAEEKRLAVTER HHHHHHHHHHHHHHH	44.19	21906983
388	Phosphorylation	EEKRLAVTERATGDL HHHHHHHHHHHHHHH	17.72	25599653
500	Ubiquitination	ATVQSRLKASAVESL HHHHHHHHHHHHHHC	38.90	21906983
502	Phosphorylation	VQSRLKASAVESLRP HHHHHHHHHHHHCCH	31.12	24719451
515	Phosphorylation	RPSQQAPSGSDLANP CHHCCCCCCCCCCCH	54.68	27251275
517	Phosphorylation	SQQAPSGSDLANPQA HCCCCCCCCCCCHHH	33.51	27251275
572	Phosphorylation	RIHSHEGTAQRLQSL CCCCCHHHHHHHHHH	19.34	20068231
583	Ubiquitination	LQSLGTEKETAQKEC HHHHCCHHHHHHHHH	61.27	-
612	Phosphorylation	QLPVALNSVKNKFSD HHHHHHHHHCCCCCC	35.01	24719451
632	Ubiquitination	SLYGEKAKAALDLER HHHCHHHHHHHHHHH	45.30	-
751	Phosphorylation	VVQDAALTYQQFKNC HHHHHHHHHHHHHCC	17.98	19060867
752	Phosphorylation	VQDAALTYQQFKNCK HHHHHHHHHHHHCCC	10.93	28152594
763	Phosphorylation	KNCKDNLSSWLEHLP HCCCCCHHHHHHHCC	26.23	30619164
764	Phosphorylation	NCKDNLSSWLEHLPR CCCCCHHHHHHHCCH	38.56	30619164
777	Phosphorylation	PRSQVRPSDGPSQIA CHHHCCCCCCHHHHH	44.64	28555341
794	Phosphorylation	LQAQKRLTQEIQSRE HHHHHHHHHHHHHHH	28.34	23898821
799	Phosphorylation	RLTQEIQSRERDRAT HHHHHHHHHHHHHHH	41.59	24719451
904	Phosphorylation	THDAKQGSESPAQAG HHHHCCCCCCHHHCC	32.19	22617229
906	Phosphorylation	DAKQGSESPAQAGRE HHCCCCCCHHHCCCH	27.51	25849741
914	Phosphorylation	PAQAGRESEALKAQL HHHCCCHHHHHHHHH	27.15	23090842
963	Phosphorylation	EKEVVEFYRDPQLEG HHHHHHHHCCHHHCC	10.70	21945579
971	Phosphorylation	RDPQLEGSLSRVKAQ CCHHHCCCHHHHHHH	17.50	21945579
973	Phosphorylation	PQLEGSLSRVKAQVE HHHCCCHHHHHHHHH	36.40	21945579
1009	Phosphorylation	QLESKRKTMQPHLLT HHHHHCCCCCCHHCC	25.68	23403867
1016	Phosphorylation	TMQPHLLTKEVTQVE CCCCHHCCCCCCCCC	31.36	21406692
1020	Phosphorylation	HLLTKEVTQVERDPG HHCCCCCCCCCCCCC	28.17	21406692
1065	Ubiquitination	KELLALEKREVDVKE HHHHHHHHCCCCCCC	55.50	-
1157	Phosphorylation	QESSRLRSLLEEERT HHHHHHHHHHHHHHH	41.73	23403867
1174	Phosphorylation	ATLARELSDLHSKYS HHHHHHHHHHHHHHH	33.11	27251275
1206	Phosphorylation	IFQVDPETEQEITRL HHCCCHHCHHHHHHH	48.64	-
1225	Phosphorylation	QEMAGKRSGVEKEVE HHHHCCCCCCHHHHH	51.15	-
1254	Phosphorylation	TVEYKEVTQEVVRHE CEECHHHHHHHHHCC	21.80	-
1263	Phosphorylation	EVVRHERSPEVLREI HHHHCCCCHHHHHHH	23.56	25159151
1352	Phosphorylation	RAAEDAVYELQSKRL HHHHHHHHHHHHHHH	16.79	28152594
1356	Phosphorylation	DAVYELQSKRLLLER HHHHHHHHHHHHHHC	32.16	20860994
1392	Phosphorylation	REEHSRLSGSLDEEV HHHHHHHCCCCCHHH	25.81	19691289
1394	Phosphorylation	EHSRLSGSLDEEVGR HHHHHCCCCCHHHHH	29.31	28857561
1451	Ubiquitination	LRIQELEKRPPTVQE HHHHHHHHCCCCHHH	80.82	-
1455	Phosphorylation	ELEKRPPTVQEKIIM HHHHCCCCHHHHHHH	37.19	22817900
1467	Ubiquitination	IIMEEVVKLEKDPDL HHHHHHHHHHCCCCH	56.98	17370265
1476	Ubiquitination	EKDPDLEKSTEALRW HCCCCHHHHHHHHCC	70.72	-
1477	Phosphorylation	KDPDLEKSTEALRWD CCCCHHHHHHHHCCC	23.31	22617229
1500	Ubiquitination	TELNRECKNLQVQID HHHHHHHHHCHHHHH	56.81	-
1511	Ubiquitination	VQIDVLQKAKSQEKT HHHHHHHHHHHCCCC	54.33	-
1514	Phosphorylation	DVLQKAKSQEKTIYK HHHHHHHHCCCCHHH	48.72	26074081
1518	Phosphorylation	KAKSQEKTIYKEVIR HHHHCCCCHHHHHHH	28.87	28152594
1520	Phosphorylation	KSQEKTIYKEVIRVQ HHCCCCHHHHHHHHH	13.31	28152594
1575	Phosphorylation	ETLGRTWSREESELQ HHHCCCCCHHHHHHH	29.24	30624053
1617	Phosphorylation	TLQLQEESKLLSQKT HHHHHHHHHHHHHCC	28.05	-
1621	Phosphorylation	QEESKLLSQKTESER HHHHHHHHHCCHHHH	39.81	-
1649	Acetylation	EAAILREKDQIYEKE HHHHHHHHHHHHHHH	48.80	19812009
1653	Phosphorylation	LREKDQIYEKERTLR HHHHHHHHHHHHHHH	18.47	28152594
1667	Phosphorylation	RDLHAKVSREELSQE HHHHHHCCHHHHHHH	33.01	24719451
1672	Phosphorylation	KVSREELSQETQTRE HCCHHHHHHHHHHCC	29.29	23312004
1698	Phosphorylation	PETGKDMSPYEAYKR CCCCCCCCHHHHHHC	34.17	21815630
1700	Phosphorylation	TGKDMSPYEAYKRGI CCCCCCHHHHHHCCC	13.09	21253578
1703	Phosphorylation	DMSPYEAYKRGIIDR CCCHHHHHHCCCCCH	6.84	22817900
1787	Phosphorylation	VAGETKPSSSLSIGS CCCCCCCCCCCCCCC	33.83	28348404
1788	Phosphorylation	AGETKPSSSLSIGSI CCCCCCCCCCCCCCH	43.77	25072903
1789	Phosphorylation	GETKPSSSLSIGSII CCCCCCCCCCCCCHH	30.39	24719451
1791	Phosphorylation	TKPSSSLSIGSIISK CCCCCCCCCCCHHCC	27.18	25072903
1794	Phosphorylation	SSSLSIGSIISKSPL CCCCCCCCHHCCCCC	18.32	25072903
1797	Phosphorylation	LSIGSIISKSPLASP CCCCCHHCCCCCCCC	25.97	25072903
1799	Phosphorylation	IGSIISKSPLASPAP CCCHHCCCCCCCCCC	20.53	20068231
1803	Phosphorylation	ISKSPLASPAPQSTS HCCCCCCCCCCCCCC	29.31	20068231
1808	Phosphorylation	LASPAPQSTSFFSPS CCCCCCCCCCCCCCC	25.59	20068231
1809	Phosphorylation	ASPAPQSTSFFSPSF CCCCCCCCCCCCCCC	25.06	20068231
1810	Phosphorylation	SPAPQSTSFFSPSFS CCCCCCCCCCCCCCC	29.55	20068231
1813	Phosphorylation	PQSTSFFSPSFSLGL CCCCCCCCCCCCCCC	20.02	20068231
1815	Phosphorylation	STSFFSPSFSLGLGD CCCCCCCCCCCCCCC	26.69	20068231
1817	Phosphorylation	SFFSPSFSLGLGDDS CCCCCCCCCCCCCCC	26.35	20068231
1865	Phosphorylation	LLEAQAATGGIVDLL HHHHHHHHCCHHHHH	38.67	20860994
1873	Phosphorylation	GGIVDLLSRERYSVH CCHHHHHCCCHHHHH	39.12	20860994
1911	Phosphorylation	TGIEDPVTKKRLSVG HCCCCCCCCCCCCHH	35.91	-
1916	Phosphorylation	PVTKKRLSVGEAVQK CCCCCCCCHHHHHHC	31.91	20068231
1988	Acetylation	DLTDPISKERLSYKE CCCCCCCHHHCCHHH	47.80	7431155
1993	Phosphorylation	ISKERLSYKEAMGRC CCHHHCCHHHHHCCC	19.89	-
2006	Phosphorylation	RCRKDPLSGLLLLPA CCCCCCCCCCCHHHH	32.55	20068231
2018	Phosphorylation	LPAALEGYRCYRSAS HHHHHCCCCCCCCCC	6.53	20068231
2023	Phosphorylation	EGYRCYRSASPTVPR CCCCCCCCCCCCCCC	13.46	26657352
2025	Phosphorylation	YRCYRSASPTVPRSL CCCCCCCCCCCCCCC	23.50	30278072
2027	Phosphorylation	CYRSASPTVPRSLR- CCCCCCCCCCCCCC-	40.42	23927012

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of EVPL_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of EVPL_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of EVPL_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PEPL_HUMAN	PPL	physical	9412476
RL27_HUMAN	RPL27	physical	22939629
K1C14_HUMAN	KRT14	physical	22939629
PM34_HUMAN	SLC25A17	physical	22939629

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.";
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.;
Anal. Sci. 24:161-166(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1455, AND MASSSPECTROMETRY.
PubMed: 18187866

Ubiquitylation

"Tryptic digestion of ubiquitin standards reveals an improved strategyfor identifying ubiquitinated proteins by mass spectrometry.";
Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D.;
Proteomics 7:868-874(2007).
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1467, AND MASSSPECTROMETRY.
PubMed: 17370265