ETAA1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ETAA1_HUMAN
• UniProt AC: Q9NY74
• Protein Name: Ewing's tumor-associated antigen 1 {ECO:0000312|HGNC:HGNC:24648}
• Gene Name: ETAA1 {ECO:0000312|HGNC:HGNC:24648}
• Organism: Homo sapiens (Human).
• Sequence Length: 926
• Subcellular Localization: Nucleus . Localizes at sites of DNA damage following replication stress (PubMed:27601467, PubMed:27723720, PubMed:27723717). Recruited to stalled replication forks via interaction with RPA1 and RPA2 subunits of the RPA complex (PubMed:27601467, PubMe
• Protein Description: Replication stress response protein that accumulates at DNA damage sites and promotes replication fork progression and integrity. [PubMed: 27601467]
• Protein Sequence: MSRRRKHDDSPSPKKTPHKTVAAEECGSVVEPGRRRLRSARGSWPCGAREGPPGPVRQREQPPTAALCSKSNPEERYETPKRALKMDSLSSSFSSPNDPDGQNDIFWDQNSPLTKQLGKGRKKQIYTTDSDEISHIVNRIAPQDEKPTTNSMLDMWIGETAIPCTPSVAKGKSRAKISCTKLKTQSQEEELMKLAKQFDKNMEELDVIQEQNKRNYDFTQMISETEILSNYKDNIQMWSLHNIVPEIDNATKKPIKGNTKISVANNQNSSQKPFDQIAEAAFNAIFDGSTQKCSGQLSQELPEAFWSTSNTTFVKTNALKEEKIITNETLVIEKLSNKTPRSLSSQVDTPIMTKSCVTSCTKEPETSNKYIDAFTTSDFEDDWENLLGSEPFAMQNIDMPELFPSKTAHVTDQKEICTFNSKTVKNTSRANTSPDARLGDSKVLQDLSSKTYDRELIDAEYRFSPNSNKSNKLSTGNKMKFENSSNKIVIQDEIQNCIVTSNLTKIKEDILTNSTEASERKSALNTRYSNEQKNKCILNQSIKAPVNTDLFGSANLGSKTSVSNPNQTSASKVGSFFDDWNDPSFANEIIKACHQLDNTWEADDVDDDLLYQACDDIERLTQQQDIRKDSKTSESICEINNNSEHGAKLTQQQDIRKDSKTSESICEINNNSEHGAKNMFAISKQGSNLVQSKHLNPGSISVQTSLTNSSQIDKPMKMEKGEMYGNSPRFLGATNLTMYSKISNCQINNLHVSYTNTDVPIQVNSSKLVLPGSSSLNVTSDHMNTEITTYKKKLSTNQPCHKTVTDEAQSNLNTTVGFSKFTFTRMKNSQILSQFNQNCITGSMSDTKITQGVEKKKGVNPLLEEAVGQQSLVKLSESLKQSSKEEEEKNRKCSPEEIQRKRQEALVRRMAKARASSVNAAPTSFL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Phosphorylation	RRRKHDDSPSPKKTP CCCCCCCCCCCCCCC	33.08	28985074
12	Phosphorylation	RKHDDSPSPKKTPHK CCCCCCCCCCCCCCC	53.99	28985074
39	Phosphorylation	PGRRRLRSARGSWPC CCCHHHHHCCCCCCC	27.23	28555341
43	Phosphorylation	RLRSARGSWPCGARE HHHHCCCCCCCCCCC	23.45	27251275
85	Sumoylation	ETPKRALKMDSLSSS CCHHHHHHHHHHCCC	39.41	28112733
95	Phosphorylation	SLSSSFSSPNDPDGQ HHCCCCCCCCCCCCC	26.22	-
119	Methylation	PLTKQLGKGRKKQIY HHHHHCCCCCCCEEE	65.22	-
122	Ubiquitination	KQLGKGRKKQIYTTD HHCCCCCCCEEEECC	58.67	-
123	Ubiquitination	QLGKGRKKQIYTTDS HCCCCCCCEEEECCH	40.33	29967540
130	Phosphorylation	KQIYTTDSDEISHIV CEEEECCHHHHHHHH	34.74	25159151
146	Acetylation	RIAPQDEKPTTNSML HHCCCCCCCCCCCHH	56.82	19818169
165	Phosphorylation	GETAIPCTPSVAKGK CCCCCCCCHHHHCCC	17.16	24719451
167	Phosphorylation	TAIPCTPSVAKGKSR CCCCCCHHHHCCCCC	19.29	28060719
178	Phosphorylation	GKSRAKISCTKLKTQ CCCCCEEEECCCCCC	18.28	-
183	Sumoylation	KISCTKLKTQSQEEE EEEECCCCCCCHHHH	46.18	-
183	Sumoylation	KISCTKLKTQSQEEE EEEECCCCCCCHHHH	46.18	-
183	Ubiquitination	KISCTKLKTQSQEEE EEEECCCCCCCHHHH	46.18	-
184	Phosphorylation	ISCTKLKTQSQEEEL EEECCCCCCCHHHHH	43.84	20363803
186	Phosphorylation	CTKLKTQSQEEELMK ECCCCCCCHHHHHHH	44.80	25159151
193	Ubiquitination	SQEEELMKLAKQFDK CHHHHHHHHHHHHHH	58.90	29967540
196	Sumoylation	EELMKLAKQFDKNME HHHHHHHHHHHHCHH	63.03	-
196	Sumoylation	EELMKLAKQFDKNME HHHHHHHHHHHHCHH	63.03	-
200	Ubiquitination	KLAKQFDKNMEELDV HHHHHHHHCHHHHHH	60.53	29967540
213	Ubiquitination	DVIQEQNKRNYDFTQ HHHHHHHHCCCCHHH	41.54	29967540
256	Acetylation	NATKKPIKGNTKISV CCCCCCCCCCCEEEE	55.42	91065
260	Sumoylation	KPIKGNTKISVANNQ CCCCCCCEEEECCCC	36.66	-
260	Sumoylation	KPIKGNTKISVANNQ CCCCCCCEEEECCCC	36.66	-
298	Phosphorylation	QKCSGQLSQELPEAF CCCCCCHHHHCCHHH	17.45	28348404
342	O-linked_Glycosylation	LSNKTPRSLSSQVDT HCCCCCCCHHHCCCC	33.53	30379171
342	Phosphorylation	LSNKTPRSLSSQVDT HCCCCCCCHHHCCCC	33.53	20363803
344	Phosphorylation	NKTPRSLSSQVDTPI CCCCCCHHHCCCCCC	21.70	20363803
345	Phosphorylation	KTPRSLSSQVDTPIM CCCCCHHHCCCCCCC	39.24	25159151
349	Phosphorylation	SLSSQVDTPIMTKSC CHHHCCCCCCCCCHH	18.83	29396449
354	Ubiquitination	VDTPIMTKSCVTSCT CCCCCCCCHHHHCCC	25.20	29967540
359	Phosphorylation	MTKSCVTSCTKEPET CCCHHHHCCCCCCCC	10.21	21712546
362	Ubiquitination	SCVTSCTKEPETSNK HHHHCCCCCCCCCCC	75.50	-
414	Sumoylation	TAHVTDQKEICTFNS CCCCCCCCCEEECCC	52.43	-
414	Sumoylation	TAHVTDQKEICTFNS CCCCCCCCCEEECCC	52.43	28112733
414	Ubiquitination	TAHVTDQKEICTFNS CCCCCCCCCEEECCC	52.43	29967540
418	Phosphorylation	TDQKEICTFNSKTVK CCCCCEEECCCCCCC	31.35	27174698
421	Phosphorylation	KEICTFNSKTVKNTS CCEEECCCCCCCCCC	26.05	27174698
422	Acetylation	EICTFNSKTVKNTSR CEEECCCCCCCCCCC	59.44	26051181
423	Phosphorylation	ICTFNSKTVKNTSRA EEECCCCCCCCCCCC	36.21	27174698
427	Phosphorylation	NSKTVKNTSRANTSP CCCCCCCCCCCCCCC	17.55	26074081
428	Phosphorylation	SKTVKNTSRANTSPD CCCCCCCCCCCCCCC	38.50	27174698
432	Phosphorylation	KNTSRANTSPDARLG CCCCCCCCCCCCCCC	40.42	26074081
433	Phosphorylation	NTSRANTSPDARLGD CCCCCCCCCCCCCCC	22.16	28985074
442	Sumoylation	DARLGDSKVLQDLSS CCCCCCHHHHHHHHC	52.26	-
442	Sumoylation	DARLGDSKVLQDLSS CCCCCCHHHHHHHHC	52.26	28112733
442	Ubiquitination	DARLGDSKVLQDLSS CCCCCCHHHHHHHHC	52.26	29967540
450	Ubiquitination	VLQDLSSKTYDRELI HHHHHHCCCCCHHHH	47.96	29967540
461	Phosphorylation	RELIDAEYRFSPNSN HHHHCCEECCCCCCC	20.76	28985074
464	Phosphorylation	IDAEYRFSPNSNKSN HCCEECCCCCCCCCC	17.74	25159151
469	Ubiquitination	RFSPNSNKSNKLSTG CCCCCCCCCCCCCCC	56.05	29967540
470	Phosphorylation	FSPNSNKSNKLSTGN CCCCCCCCCCCCCCC	43.37	23532336
480	Sumoylation	LSTGNKMKFENSSNK CCCCCCEEEECCCCC	51.65	28112733
507	Sumoylation	TSNLTKIKEDILTNS CCCCHHHHHHHHHCC	51.38	-
507	Sumoylation	TSNLTKIKEDILTNS CCCCHHHHHHHHHCC	51.38	28112733
507	Ubiquitination	TSNLTKIKEDILTNS CCCCHHHHHHHHHCC	51.38	29967540
514	Phosphorylation	KEDILTNSTEASERK HHHHHHCCCHHHHHH	23.53	25627689
528	Phosphorylation	KSALNTRYSNEQKNK HHHHHHCCCHHHHCC	17.51	-
529	Phosphorylation	SALNTRYSNEQKNKC HHHHHCCCHHHHCCE	30.51	25159151
533	Sumoylation	TRYSNEQKNKCILNQ HCCCHHHHCCEEECC	52.80	28112733
535	Sumoylation	YSNEQKNKCILNQSI CCHHHHCCEEECCCC	29.68	-
535	Sumoylation	YSNEQKNKCILNQSI CCHHHHCCEEECCCC	29.68	28112733
535	Ubiquitination	YSNEQKNKCILNQSI CCHHHHCCEEECCCC	29.68	29967540
541	Phosphorylation	NKCILNQSIKAPVNT CCEEECCCCCCCCCC	25.61	25159151
543	Sumoylation	CILNQSIKAPVNTDL EEECCCCCCCCCCCC	52.46	-
543	Sumoylation	CILNQSIKAPVNTDL EEECCCCCCCCCCCC	52.46	-
543	Ubiquitination	CILNQSIKAPVNTDL EEECCCCCCCCCCCC	52.46	-
553	Phosphorylation	VNTDLFGSANLGSKT CCCCCCCCCCCCCCC	13.47	25159151
559	Ubiquitination	GSANLGSKTSVSNPN CCCCCCCCCCCCCCC	42.92	29967540
572	Ubiquitination	PNQTSASKVGSFFDD CCCCCHHHHHHHCCC	50.78	21963094
575	Phosphorylation	TSASKVGSFFDDWND CCHHHHHHHCCCCCC	26.23	27732954
584	Phosphorylation	FDDWNDPSFANEIIK CCCCCCHHHHHHHHH	39.59	27732954
599	Phosphorylation	ACHQLDNTWEADDVD HHHHCCCCCCCCCCC	25.09	21712546
611	Phosphorylation	DVDDDLLYQACDDIE CCCHHHHHHHHHHHH	11.02	27642862
631	Sumoylation	QDIRKDSKTSESICE HHHHHCCCCCHHHEE	67.23	-
631	Sumoylation	QDIRKDSKTSESICE HHHHHCCCCCHHHEE	67.23	-
648	Acetylation	NNSEHGAKLTQQQDI CCCHHHCCCHHHHHH	57.57	26051181
648	Ubiquitination	NNSEHGAKLTQQQDI CCCHHHCCCHHHHHH	57.57	29967540
660	Sumoylation	QDIRKDSKTSESICE HHHHHCCCCCHHEEH	67.23	-
660	Sumoylation	QDIRKDSKTSESICE HHHHHCCCCCHHEEH	67.23	-
684	Sumoylation	KNMFAISKQGSNLVQ CCEEEEECCCCCCEE	53.19	-
684	Sumoylation	KNMFAISKQGSNLVQ CCEEEEECCCCCCEE	53.19	-
684	Ubiquitination	KNMFAISKQGSNLVQ CCEEEEECCCCCCEE	53.19	29967540
717	Sumoylation	SQIDKPMKMEKGEMY HHCCCCEEECCCCCC	53.05	-
717	Sumoylation	SQIDKPMKMEKGEMY HHCCCCEEECCCCCC	53.05	-
720	Ubiquitination	DKPMKMEKGEMYGNS CCCEEECCCCCCCCC	57.59	-
727	Phosphorylation	KGEMYGNSPRFLGAT CCCCCCCCCCCCCCC	17.06	-
740	Phosphorylation	ATNLTMYSKISNCQI CCCCEEEEECCCCEE	16.82	24719451
795	Phosphorylation	TTYKKKLSTNQPCHK EEEHHHCCCCCCCCC	33.90	25159151
796	Phosphorylation	TYKKKLSTNQPCHKT EEHHHCCCCCCCCCC	48.59	25159151
802	Ubiquitination	STNQPCHKTVTDEAQ CCCCCCCCCCCHHHH	51.94	29967540
833	Phosphorylation	MKNSQILSQFNQNCI ECHHHHHHHHHCCCC	34.06	28112733
856	Ubiquitination	ITQGVEKKKGVNPLL CCCCCHHHCCCCHHH	41.05	29967540
857	Ubiquitination	TQGVEKKKGVNPLLE CCCCHHHCCCCHHHH	78.23	29967540
874	Ubiquitination	VGQQSLVKLSESLKQ HCCHHHHHHHHHHHH	52.14	29967540
880	Ubiquitination	VKLSESLKQSSKEEE HHHHHHHHHHCHHHH	58.37	29967540
891	Methylation	KEEEEKNRKCSPEEI HHHHHHHHCCCHHHH	53.04	-
894	Phosphorylation	EEKNRKCSPEEIQRK HHHHHCCCHHHHHHH	38.08	23663014
916	Phosphorylation	RMAKARASSVNAAPT HHHHHHHHHCCCCCC	29.30	22617229
917	Phosphorylation	MAKARASSVNAAPTS HHHHHHHHCCCCCCC	20.57	28555341

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
95	S	Phosphorylation	Kinase	ATR	Q13535	PSP
95	S	Phosphorylation	Kinase	CDK2	P24941	PSP
111	S	Phosphorylation	Kinase	ATR	Q13535	PSP
111	S	Phosphorylation	Kinase	CDK2	P24941	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ETAA1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ETAA1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TZAP_HUMAN	ZBTB48	physical	26496610
SP16H_HUMAN	SUPT16H	physical	26496610
SYTC2_HUMAN	TARSL2	physical	26496610

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage.";
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
Science 316:1160-1166(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-833, AND MASSSPECTROMETRY.
PubMed: 17525332