EIN2_ARATH - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: EIN2_ARATH
• UniProt AC: Q9S814
• Protein Name: Ethylene-insensitive protein 2 {ECO:0000303|PubMed:10381874}
• Gene Name: EIN2 {ECO:0000303|PubMed:10381874}
• Organism: Arabidopsis thaliana (Mouse-ear cress).
• Sequence Length: 1294
• Subcellular Localization: Ethylene-insensitive protein 2: Endoplasmic reticulum membrane ; Multi-pass membrane protein .; EIN2-CEND: Nucleus . Cytoplasm . Endoplasmic reticulum membrane . Perception of ethylene or methyl jasmonate leads to proteolytic cleavage allowing the C-
• Protein Description: Central factor in signaling pathways regulated by ethylene (ET), and involved in various processes including development, plant defense, senescence, nucleotide sugar flux, and tropisms. Necessary for ethylene-mediated gene regulation, and for the induction of some genes by ozone. Acts downstream of ET receptors, and upstream of ethylene regulated transcription factors. Required for cytokinin-mediated processes. Seems to be implicated in cross-talk between ET, jasmonate and other pathways. Probably not involved in iron uptake. [PubMed: 10381874]
• Protein Sequence: MEAEIVNVRPQLGFIQRMVPALLPVLLVSVGYIDPGKWVANIEGGARFGYDLVAITLLFNFAAILCQYVAARISVVTGKHLAQICNEEYDKWTCMFLGIQAEFSAILLDLTMVVGVAHALNLLFGVELSTGVFLAAMDAFLFPVFASFLENGMANTVSIYSAGLVLLLYVSGVLLSQSEIPLSMNGVLTRLNGESAFALMGLLGASIVPHNFYIHSYFAGESTSSSDVDKSSLCQDHLFAIFGVFSGLSLVNYVLMNAAANVFHSTGLVVLTFHDALSLMEQVFMSPLIPVVFLMLLFFSSQITALAWAFGGEVVLHDFLKIEIPAWLHRATIRILAVAPALYCVWTSGADGIYQLLIFTQVLVAMMLPCSVIPLFRIASSRQIMGVHKIPQVGEFLALTTFLGFLGLNVVFVVEMVFGSSDWAGGLRWNTVMGTSIQYTTLLVSSCASLCLILWLAATPLKSASNRAEAQIWNMDAQNALSYPSVQEEEIERTETRRNEDESIVRLESRVKDQLDTTSVTSSVYDLPENILMTDQEIRSSPPEERELDVKYSTSQVSSLKEDSDVKEQSVLQSTVVNEVSDKDLIVETKMAKIEPMSPVEKIVSMENNSKFIEKDVEGVSWETEEATKAAPTSNFTVGSDGPPSFRSLSGEGGSGTGSLSRLQGLGRAARRHLSAILDEFWGHLYDFHGQLVAEARAKKLDQLFGTDQKSASSMKADSFGKDISSGYCMSPTAKGMDSQMTSSLYDSLKQQRTPGSIDSLYGLQRGSSPSPLVNRMQMLGAYGNTTNNNNAYELSERRYSSLRAPSSSEGWEHQQPATVHGYQMKSYVDNLAKERLEALQSRGEIPTSRSMALGTLSYTQQLALALKQKSQNGLTPGPAPGFENFAGSRSISRQSERSYYGVPSSGNTDTVGAAVANEKKYSSMPDISGLSMSARNMHLPNNKSGYWDPSSGGGGYGASYGRLSNESSLYSNLGSRVGVPSTYDDISQSRGGYRDAYSLPQSATTGTGSLWSRQPFEQFGVAERNGAVGEELRNRSNPINIDNNASSNVDAEAKLLQSFRHCILKLIKLEGSEWLFGQSDGVDEELIDRVAAREKFIYEAEAREINQVGHMGEPLISSVPNCGDGCVWRADLIVSFGVWCIHRVLDLSLMESRPELWGKYTYVLNRLQGVIDPAFSKLRTPMTPCFCLQIPASHQRASPTSANGMLPPAAKPAKGKCTTAVTLLDLIKDVEMAISCRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPVGMNQDGPGSRKNVTAYGSLG

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
503	Phosphorylation	TRRNEDESIVRLESR HCCCCCCCHHHHHHH	38.41	30407730
509	Phosphorylation	ESIVRLESRVKDQLD CCHHHHHHHHHHHCC	47.14	30407730
555	Phosphorylation	LDVKYSTSQVSSLKE CCCCCCHHHHHCCCC	23.73	29797451
598	Phosphorylation	MAKIEPMSPVEKIVS CCCCCCCCCHHHHHC	36.19	27288362
605	Phosphorylation	SPVEKIVSMENNSKF CCHHHHHCCCCCCCC	24.69	30407730
633	Phosphorylation	EATKAAPTSNFTVGS HHHCCCCCCCCCCCC	31.41	23776212
634	Phosphorylation	ATKAAPTSNFTVGSD HHCCCCCCCCCCCCC	28.96	23776212
637	Phosphorylation	AAPTSNFTVGSDGPP CCCCCCCCCCCCCCC	28.36	23776212
640	Phosphorylation	TSNFTVGSDGPPSFR CCCCCCCCCCCCCCC	34.75	23776212
645	Phosphorylation	VGSDGPPSFRSLSGE CCCCCCCCCCCCCCC	36.10	30291188
648	Phosphorylation	DGPPSFRSLSGEGGS CCCCCCCCCCCCCCC	25.52	23776212
650	Phosphorylation	PPSFRSLSGEGGSGT CCCCCCCCCCCCCCC	35.72	23776212
655	Phosphorylation	SLSGEGGSGTGSLSR CCCCCCCCCCCHHHH	44.00	30291188
657	Phosphorylation	SGEGGSGTGSLSRLQ CCCCCCCCCHHHHHH	25.87	23776212
659	Phosphorylation	EGGSGTGSLSRLQGL CCCCCCCHHHHHHHH	24.02	30291188
661	Phosphorylation	GSGTGSLSRLQGLGR CCCCCHHHHHHHHHH	32.42	23776212
731	Phosphorylation	ISSGYCMSPTAKGMD CCCCCCCCCCCCCCC	18.61	23776212
733	Phosphorylation	SGYCMSPTAKGMDSQ CCCCCCCCCCCCCHH	33.42	23776212
754	Phosphorylation	DSLKQQRTPGSIDSL HHHHHCCCCCCHHHH	27.88	29654922
757	Phosphorylation	KQQRTPGSIDSLYGL HHCCCCCCHHHHHCC	25.15	19880383
760	Phosphorylation	RTPGSIDSLYGLQRG CCCCCHHHHHCCCCC	23.09	25561503
769	Phosphorylation	YGLQRGSSPSPLVNR HCCCCCCCCCHHHHH	32.02	25561503
819	Phosphorylation	WEHQQPATVHGYQMK CCCCCCCEECHHHHH	21.51	19880383
871	Phosphorylation	ALALKQKSQNGLTPG HHHHHHHHHCCCCCC	26.78	30589143
876	Phosphorylation	QKSQNGLTPGPAPGF HHHHCCCCCCCCCCC	27.83	30407730
922	Phosphorylation	AVANEKKYSSMPDIS HHCCCCCCCCCCCCC	19.62	25561503
923	Phosphorylation	VANEKKYSSMPDISG HCCCCCCCCCCCCCC	29.10	25561503
924	Phosphorylation	ANEKKYSSMPDISGL CCCCCCCCCCCCCCC	31.11	19880383
1199	Phosphorylation	PASHQRASPTSANGM CCCCCCCCCCCCCCC	30.79	30291188
1201	Phosphorylation	SHQRASPTSANGMLP CCCCCCCCCCCCCCC	37.67	25561503
1283	Phosphorylation	MNQDGPGSRKNVTAY CCCCCCCCCCCCEEC	43.12	30291188
1292	Phosphorylation	KNVTAYGSLG----- CCCEECCCCC-----	19.76	29654922

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of EIN2_ARATH !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
645	S	Phosphorylation	Phosphorylation of Ser-645 and Ser-924 is involved in repressing EIN2 signaling.	23132950
924	S	Phosphorylation	Phosphorylation of Ser-645 and Ser-924 is involved in repressing EIN2 signaling.	23132950

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of EIN2_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ETR1_ARATH	ETR1	physical	19769567
CTR1_ARATH	CTR1	physical	23132950
ETR1_ARATH	ETR1	physical	20591837
ERS1_ARATH	ERS1	physical	20591837
ETR2_ARATH	ETR2	physical	20591837
ERS2_ARATH	ERS2	physical	20591837
EIN4_ARATH	EIN4	physical	20591837
FB162_ARATH	ETP1	physical	19196655
FB161_ARATH	ETP2	physical	19196655

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.