DGKH_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DGKH_HUMAN
• UniProt AC: Q86XP1
• Protein Name: Diacylglycerol kinase eta
• Gene Name: DGKH
• Organism: Homo sapiens (Human).
• Sequence Length: 1220
• Subcellular Localization: Cytoplasm . Cell membrane . Translocated from the cytoplasm to endosomes in response to stress stimuli (PubMed:12810723). Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endoso
• Protein Description: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization..
• Protein Sequence: MAGAGGQHHPPGAAGGAAAGAGAAVTSAAASAGPGEDSSDSEAEQEGPQKLIRKVSTSGQIRTKTSIKEGQLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAEASTKNANNSFTIITPFRRLMLCAENRKEMEDWISSLKSVQTREPYEVAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWKCLWCKTMVHTACKDLYHPICPLGQCKVSIIPPIALNSTDSDGFCRATFSFCVSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLNLNKQCQLGVLPLGTGNDLARVLGWGGSYDDDTQLPQILEKLERASTKMLDRWSIMTYELKLPPKASLLPGPPEASEEFYMTIYEDSVATHLTKILNSDEHAVVISSAKTLCETVKDFVAKVEKTYDKTLENAVVADAVASKCSVLNEKLEQLLQALHTDSQAAPVLPGLSPLIVEEDAVESSSEESLGESKEQLGDDVTKPSSQKAVKPREIMLRANSLKKAVRQVIEEAGKVMDDPTVHPCEPANQSSDYDSTETDESKEEAKDDGAKESITVKTAPRSPDARASYGHSQTDSVPGPAVAASKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPFGATPFIDPDLDSVDGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNLMWYGVLGTRELLQRSYKNLEQRVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFAAPSFDDKILEVVAIFDSMQMAVSRVIKLQHHRIAQCRTVKITIFGDEGVPVQVDGEAWVQPPGIIKIVHKNRAQMLTRDRAFESTLKSWEDKQKCDSGKPVLRTHLYIHHAIDLATEEVSQMQLCSQAAEELITRICDAATIHCLLEQELAHAVNACSHALNKANPRCPESLTRDTATEIAINVKALYNETESLLVGRVPLQLESPHEERVSNALHSVEVELQKLTEIPWLYYILHPNEDEEPPMDCTKRNNRSTVFRIVPKFKKEKVQKQKTSSQPVQKWGTEEVAAWLDLLNLGEYKDIFIRHDIRGAELLHLERRDLKDLGIPKVGHVKRILQGIKELGRSTPQSEV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15 (in isoform 5)	Phosphorylation	-	26.24	29507054
15 (in isoform 3)	Phosphorylation	-	26.24	29507054
26	Phosphorylation	AGAGAAVTSAAASAG CCHHHHHHHHHHHCC	14.12	20873877
27	Phosphorylation	GAGAAVTSAAASAGP CHHHHHHHHHHHCCC	14.87	20873877
31	Phosphorylation	AVTSAAASAGPGEDS HHHHHHHHCCCCCCC	29.09	20873877
38	Phosphorylation	SAGPGEDSSDSEAEQ HCCCCCCCCCHHHHH	31.57	20873877
39	Phosphorylation	AGPGEDSSDSEAEQE CCCCCCCCCHHHHHH	58.43	20873877
41	Phosphorylation	PGEDSSDSEAEQEGP CCCCCCCHHHHHHCH	40.43	20873877
56	Phosphorylation	QKLIRKVSTSGQIRT HHHHHHHCCCCCEEE	21.64	23401153
57	Phosphorylation	KLIRKVSTSGQIRTK HHHHHHCCCCCEEEC	39.60	30266825
58	Phosphorylation	LIRKVSTSGQIRTKT HHHHHCCCCCEEECC	22.61	30266825
65	Phosphorylation	SGQIRTKTSIKEGQL CCCEEECCCCCCCCH	34.76	-
74	Ubiquitination	IKEGQLLKQTSSFQR CCCCCHHHCCCHHHH	61.19	-
111	Phosphorylation	IFDEVDLSDASVAEA EEEECCCCCCHHHHH	27.02	28450419
114	Phosphorylation	EVDLSDASVAEASTK ECCCCCCHHHHHHCC	27.18	28450419
119	Phosphorylation	DASVAEASTKNANNS CCHHHHHHCCCCCCC	31.27	28450419
120	Phosphorylation	ASVAEASTKNANNSF CHHHHHHCCCCCCCE	35.05	28450419
126	Phosphorylation	STKNANNSFTIITPF HCCCCCCCEEEEECH	24.47	20873877
128	Phosphorylation	KNANNSFTIITPFRR CCCCCCEEEEECHHH	15.99	20873877
151	Phosphorylation	KEMEDWISSLKSVQT HHHHHHHHHCCCCCC	25.93	24719451
152	Phosphorylation	EMEDWISSLKSVQTR HHHHHHHHCCCCCCC	30.59	28270605
266	"N6,N6-dimethyllysine"	AKCAVCDKTCGSVLR CEEEECCCCCCCEEE	40.36	-
278	Acetylation	VLRLQDWKCLWCKTM EEECCCCEEHHHHHH	27.95	25953088
306	Phosphorylation	PLGQCKVSIIPPIAL CCCCCEEEECCCEEC	10.57	20873877
315	Phosphorylation	IPPIALNSTDSDGFC CCCEECCCCCCCCCC	34.15	28348404
316	Phosphorylation	PPIALNSTDSDGFCR CCEECCCCCCCCCCH	38.15	28348404
318	Phosphorylation	IALNSTDSDGFCRAT EECCCCCCCCCCHHH	39.46	27251275
348	Ubiquitination	SGDNQGVKFLRRFKQ CCCCHHHHHHHHHHH	45.57	-
408	Ubiquitination	IDKLNLNKQCQLGVL HHHCCCCCCCCCCCC	56.47	-
445	Ubiquitination	QLPQILEKLERASTK HHHHHHHHHHHHCCH	52.80	-
450	Phosphorylation	LEKLERASTKMLDRW HHHHHHHCCHHHHCC	34.48	23403867
451	Phosphorylation	EKLERASTKMLDRWS HHHHHHCCHHHHCCE	21.35	-
488	Phosphorylation	EEFYMTIYEDSVATH CCEEEHHHHHHHHHH	12.43	27642862
513	Ubiquitination	AVVISSAKTLCETVK EEEEECHHHHHHHHH	43.93	-
532	Ubiquitination	KVEKTYDKTLENAVV HHHHHHCHHHHHHHH	44.59	-
533	Phosphorylation	VEKTYDKTLENAVVA HHHHHCHHHHHHHHH	36.08	-
545	Phosphorylation	VVADAVASKCSVLNE HHHHHHHHHHHHHHH	27.83	-
546	Ubiquitination	VADAVASKCSVLNEK HHHHHHHHHHHHHHH	22.31	-
604	Phosphorylation	EQLGDDVTKPSSQKA HHHCCCCCCCCCCCC	44.20	25022875
607	Phosphorylation	GDDVTKPSSQKAVKP CCCCCCCCCCCCCCH	47.13	25022875
608	Phosphorylation	DDVTKPSSQKAVKPR CCCCCCCCCCCCCHH	44.20	25159151
623	Phosphorylation	EIMLRANSLKKAVRQ HHHHHHHHHHHHHHH	40.35	29514088
643	Phosphorylation	GKVMDDPTVHPCEPA CCCCCCCCCCCCCCC	38.14	28796482
653	Phosphorylation	PCEPANQSSDYDSTE CCCCCCCCCCCCCCC	25.61	24114839
654	Phosphorylation	CEPANQSSDYDSTET CCCCCCCCCCCCCCC	30.35	24114839
656	Phosphorylation	PANQSSDYDSTETDE CCCCCCCCCCCCCHH	17.47	28796482
658	Phosphorylation	NQSSDYDSTETDESK CCCCCCCCCCCHHHH	23.16	24114839
659	Phosphorylation	QSSDYDSTETDESKE CCCCCCCCCCHHHHH	39.75	28796482
661	Phosphorylation	SDYDSTETDESKEEA CCCCCCCCHHHHHHH	45.19	28796482
664	Phosphorylation	DSTETDESKEEAKDD CCCCCHHHHHHHCCC	48.45	28796482
676	Phosphorylation	KDDGAKESITVKTAP CCCCCCCCEEEEECC	23.89	26074081
678	Phosphorylation	DGAKESITVKTAPRS CCCCCCEEEEECCCC	25.81	26074081
681	Phosphorylation	KESITVKTAPRSPDA CCCEEEEECCCCCCC	37.24	24719451
685	Phosphorylation	TVKTAPRSPDARASY EEEECCCCCCCHHHC	26.52	25849741
691	Phosphorylation	RSPDARASYGHSQTD CCCCCHHHCCCCCCC	26.54	25159151
692	Phosphorylation	SPDARASYGHSQTDS CCCCHHHCCCCCCCC	20.20	26074081
695	Phosphorylation	ARASYGHSQTDSVPG CHHHCCCCCCCCCCC	30.34	25159151
697	Phosphorylation	ASYGHSQTDSVPGPA HHCCCCCCCCCCCHH	33.00	26074081
699	Phosphorylation	YGHSQTDSVPGPAVA CCCCCCCCCCCHHCC	33.33	26074081
708	Phosphorylation	PGPAVAASKENLPVL CCHHCCCCCCCCCCC	30.66	26074081
717	Phosphorylation	ENLPVLNTRIICPGL CCCCCCCEEEECCCH	21.05	20068231
731	Phosphorylation	LRAGLAASIAGSSII HHHHHHHHHHCHHHH	13.83	27174698
735	Phosphorylation	LAASIAGSSIINKML HHHHHHCHHHHHHHH	14.89	27174698
736	Phosphorylation	AASIAGSSIINKMLL HHHHHCHHHHHHHHH	26.94	27174698
784	Phosphorylation	IGLDAKISLEFNNKR EECCEEEEEEECCCC	22.57	29083192
808	Phosphorylation	RTKNLMWYGVLGTRE HCHHHHHHHHHHHHH	5.38	27642862
822	Ubiquitination	ELLQRSYKNLEQRVQ HHHHHHHCCHHHHEE	57.19	-
957	Ubiquitination	RAFESTLKSWEDKQK HHHHHHHHHHHHHHC	54.32	-
1033	Ubiquitination	ACSHALNKANPRCPE HHHHHHHHHCCCCCC	50.84	-
1048	Phosphorylation	SLTRDTATEIAINVK CCCCCCHHHHHHHHH	29.98	30576142
1075	Phosphorylation	RVPLQLESPHEERVS CCCCCCCCCCHHHHH	39.90	25159151
1125	Phosphorylation	TKRNNRSTVFRIVPK CCCCCCCCEEEEEHH	22.04	24719451
1161 (in isoform 2)	Phosphorylation	-	38.11	23090842
1191	Trimethylation	HLERRDLKDLGIPKV EEECCCHHHCCCCCH	56.02	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of DGKH_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DGKH_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DGKH_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DGKH_HUMAN	DGKH	physical	12810723
DGKD_HUMAN	DGKD	physical	12810723
TRAF5_HUMAN	TRAF5	physical	21988832

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.