CLSPN_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CLSPN_MOUSE
• UniProt AC: Q80YR7
• Protein Name: Claspin
• Gene Name: Clspn
• Organism: Mus musculus (Mouse).
• Sequence Length: 1315
• Subcellular Localization: Nucleus. Chromosome.
• Protein Description: Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks (By similarity)..
• Protein Sequence: MTGEVGSEVNLEVNDLKLLSQEAADSPVDSGQGSFETLEPLSERDSDEEIFVSKKPKSRKVLQDSDSEAEDRDDAPEKPTYDDSAEDTQENLHSGKSQSRSFPKALADSDESDMEETPSQESPETQEAPSLEPGHQTGHSVDFTTGRKLSKTLLREGAEGKAKSKRRLEKEERTMEKIRRLKKKETRCEESDADRPLNDSGCLLEDSDLFETGLEEENDSALEDEESLESIRAAVKNKVKNRKKKEPTLESEAFSLEDGNELSKGSARKERKAARLSKEALKKLHSETQRLVRESALNLPYHMPESKTIHDFFKRKPRPTCQGSAMALLKSCKYQSGHYKETVNPADAAGMGAEDSSRGSEQRTGAGIAAETNVLSEVSEEAGITAGSDEACGKDPVRRGELEIEETEKHSDDRPYSPGDRSMSQQESSIPRIEDNEGHQAGDLTESDPPALEGEELKTVEKTDAKEGMPEQKTQSAAAAAVAVVTAAAAPPEKVRRFTVDRLRQLGVDVSSQPRLGADEDSFVILDEPKTNRELEALKQRFWRHANPAASPRACQTVNVNIIVKDLGTNGKEELKAEVVPVTLAAEKLEGASHAKPGEKLQMLKAKLQEAMKLRRLEERQKRQALFKLDNEDGFEEEEEEEEMTDESEEDGEEETTEYLLGSEDTETKDEKETDKENTDTSSDIGKSVALCVPKPLSSDSTLLLFKDSSSKMGYFPTEEKSETDEYLAKQSDKLDEDDSSSLLTKESSHNSSFELIGSTIPSYQPCNRQIGRGASFLPTAGFRSPSPGLFRGSLISSASKSSGKLSEPSLPVEDSQDLYTASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNIRNHRHRYQAVKPQLPLASMDENAMDANMDELLDLCTGQFTSQPEEKCQPRKNDKKENMEELLNLCSGKFPTQDASPVAPLGLRSQEKESSTEDPMEEALALCSGSFPTDREEEGEEEEFGDFQLVSKENGFASDEDEHSDSNDEELALDLEDDEEELLKQSEKMKRQMRLKKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELESQIKKIHMKTMLDDDKRRLRLYQERYLADGDLHSDGPGRTRKFRWKHIDDTSQMDLFHRDSDDDQVEEQLDETEAKWRKERIEREQWLREQAQQGKIAADEEDIGDDSQFMMLAKKVTAKALQKNASHTVVVQESKSVLRNPFETIRPGGAHQLKTGSLLNQPKAVLQKLAALSDLNPSAPRNSRNFVFHTLSPTKAEAAKDSSKPQVRRRGLSSMMSPSPKRLKTNGSSPGPKRSIFRYLES

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
26	Phosphorylation	LSQEAADSPVDSGQG HCHHHCCCCCCCCCC	23.30	25619855
34	Phosphorylation	PVDSGQGSFETLEPL CCCCCCCCCCCCCCC	16.42	25619855
37	Phosphorylation	SGQGSFETLEPLSER CCCCCCCCCCCCCCC	34.12	25619855
42	Phosphorylation	FETLEPLSERDSDEE CCCCCCCCCCCCCCC	41.10	25619855
46	Phosphorylation	EPLSERDSDEEIFVS CCCCCCCCCCCEEEC	53.82	21082442
53	Phosphorylation	SDEEIFVSKKPKSRK CCCCEEECCCCCCCC	24.98	25619855
65	Phosphorylation	SRKVLQDSDSEAEDR CCCCCCCCCCCCCCC	30.03	26525534
67	Phosphorylation	KVLQDSDSEAEDRDD CCCCCCCCCCCCCCC	42.53	26525534
80	Phosphorylation	DDAPEKPTYDDSAED CCCCCCCCCCCCHHH	51.13	25777480
81	Phosphorylation	DAPEKPTYDDSAEDT CCCCCCCCCCCHHHH	26.70	25777480
84	Phosphorylation	EKPTYDDSAEDTQEN CCCCCCCCHHHHHHH	30.57	19060867
88	Phosphorylation	YDDSAEDTQENLHSG CCCCHHHHHHHHHCC	29.32	25777480
94	Phosphorylation	DTQENLHSGKSQSRS HHHHHHHCCCCCCCC	51.59	25777480
109	Phosphorylation	FPKALADSDESDMEE CCHHHCCCCHHHCCC	37.36	22817900
112	Phosphorylation	ALADSDESDMEETPS HHCCCCHHHCCCCCC	47.44	22817900
117	Phosphorylation	DESDMEETPSQESPE CHHHCCCCCCCCCCC	18.22	22802335
119	Phosphorylation	SDMEETPSQESPETQ HHCCCCCCCCCCCCC	54.74	22817900
220	Phosphorylation	GLEEENDSALEDEES CCCCCCCCCCCCHHH	46.46	-
255	Phosphorylation	TLESEAFSLEDGNEL CCCCCCEECCCCCCC	37.59	-
263	Phosphorylation	LEDGNELSKGSARKE CCCCCCCCCCHHHHH	28.88	25159016
266	Phosphorylation	GNELSKGSARKERKA CCCCCCCHHHHHHHH	28.74	25159016
356	Phosphorylation	AGMGAEDSSRGSEQR CCCCCCCCCCCCCCC	17.28	25266776
357	Phosphorylation	GMGAEDSSRGSEQRT CCCCCCCCCCCCCCC	53.64	25266776
407	Phosphorylation	GELEIEETEKHSDDR CCEEEEECHHCCCCC	37.23	25777480
411	Phosphorylation	IEETEKHSDDRPYSP EEECHHCCCCCCCCC	52.88	28066266
416	Phosphorylation	KHSDDRPYSPGDRSM HCCCCCCCCCCCCCC	28.52	25619855
417	Phosphorylation	HSDDRPYSPGDRSMS CCCCCCCCCCCCCCC	25.70	26824392
422	Phosphorylation	PYSPGDRSMSQQESS CCCCCCCCCCCCCCC	27.76	28066266
424	Phosphorylation	SPGDRSMSQQESSIP CCCCCCCCCCCCCCC	29.93	25263469
512	Phosphorylation	QLGVDVSSQPRLGAD HCCCCCCCCCCCCCC	44.01	27841257
522	Phosphorylation	RLGADEDSFVILDEP CCCCCCCCEEEECCC	21.26	26824392
551	Phosphorylation	RHANPAASPRACQTV HHCCCCCCCCCCCEE	19.61	25266776
682	Phosphorylation	DKENTDTSSDIGKSV CCCCCCCCCHHHHHE	28.90	27841257
683	Phosphorylation	KENTDTSSDIGKSVA CCCCCCCCHHHHHEE	35.03	27841257
698	Phosphorylation	LCVPKPLSSDSTLLL EEEECCCCCCCEEEE	40.40	26745281
699	Phosphorylation	CVPKPLSSDSTLLLF EEECCCCCCCEEEEE	43.17	26745281
701	Phosphorylation	PKPLSSDSTLLLFKD ECCCCCCCEEEEEEC	23.93	26745281
702	Phosphorylation	KPLSSDSTLLLFKDS CCCCCCCEEEEEECC	26.81	26745281
709	Phosphorylation	TLLLFKDSSSKMGYF EEEEEECCCCCCCCC	36.78	-
718	Phosphorylation	SKMGYFPTEEKSETD CCCCCCCCCCCCHHH	47.05	26643407
722	Phosphorylation	YFPTEEKSETDEYLA CCCCCCCCHHHHHHH	49.35	25266776
724	Phosphorylation	PTEEKSETDEYLAKQ CCCCCCHHHHHHHHH	41.02	25266776
740	Phosphorylation	DKLDEDDSSSLLTKE HCCCCCCCCCCCCCC	33.86	27841257
741	Phosphorylation	KLDEDDSSSLLTKES CCCCCCCCCCCCCCC	31.81	27600695
742	Phosphorylation	LDEDDSSSLLTKESS CCCCCCCCCCCCCCC	31.20	22817900
748	Phosphorylation	SSLLTKESSHNSSFE CCCCCCCCCCCCCEE	38.15	23984901
749	Phosphorylation	SLLTKESSHNSSFEL CCCCCCCCCCCCEEE	27.98	23984901
752	Phosphorylation	TKESSHNSSFELIGS CCCCCCCCCEEECCC	30.86	26745281
753	Phosphorylation	KESSHNSSFELIGST CCCCCCCCEEECCCC	28.23	25266776
759	Phosphorylation	SSFELIGSTIPSYQP CCEEECCCCCCCCCC	19.03	23984901
760	Phosphorylation	SFELIGSTIPSYQPC CEEECCCCCCCCCCC	31.52	23984901
776	Phosphorylation	RQIGRGASFLPTAGF CCCCCCCCCCCCCCC	30.09	26745281
780	Phosphorylation	RGASFLPTAGFRSPS CCCCCCCCCCCCCCC	41.37	26745281
785	Phosphorylation	LPTAGFRSPSPGLFR CCCCCCCCCCCCCCC	27.71	22942356
787	Phosphorylation	TAGFRSPSPGLFRGS CCCCCCCCCCCCCHH	32.61	26824392
794	Phosphorylation	SPGLFRGSLISSASK CCCCCCHHHHHHCCC	20.48	26745281
797	Phosphorylation	LFRGSLISSASKSSG CCCHHHHHHCCCCCC	25.74	28066266
798	Phosphorylation	FRGSLISSASKSSGK CCHHHHHHCCCCCCC	29.57	28066266
800	Phosphorylation	GSLISSASKSSGKLS HHHHHHCCCCCCCCC	34.71	28066266
810	Phosphorylation	SGKLSEPSLPVEDSQ CCCCCCCCCCCCCCC	41.05	-
816	Phosphorylation	PSLPVEDSQDLYTAS CCCCCCCCCCCCCCC	16.46	21082442
820	Phosphorylation	VEDSQDLYTASPEPK CCCCCCCCCCCCCCC	14.46	25619855
821	Phosphorylation	EDSQDLYTASPEPKT CCCCCCCCCCCCCCE	28.14	25619855
823	Phosphorylation	SQDLYTASPEPKTLF CCCCCCCCCCCCEEE	23.23	22942356
868	Acetylation	RHRYQAVKPQLPLAS HHHHHCCCCCCCCCC	30.02	-
893	Phosphorylation	DELLDLCTGQFTSQP HHHHHHHCCCCCCCH	41.12	-
923	Phosphorylation	EELLNLCSGKFPTQD HHHHHHHCCCCCCCC	48.46	26745281
928	Phosphorylation	LCSGKFPTQDASPVA HHCCCCCCCCCCCCC	42.06	23970565
932	Phosphorylation	KFPTQDASPVAPLGL CCCCCCCCCCCCCCC	28.35	25338131
990	Phosphorylation	SKENGFASDEDEHSD ECCCCCCCCCCCCCC	38.99	24224561
996	Phosphorylation	ASDEDEHSDSNDEEL CCCCCCCCCCCHHHH	40.46	24224561
998	Phosphorylation	DEDEHSDSNDEELAL CCCCCCCCCHHHHHH	49.55	24224561
1094	Phosphorylation	DKRRLRLYQERYLAD HHHHHHHHHHHHCCC	11.24	22817900
1123	Phosphorylation	RWKHIDDTSQMDLFH EEECCCCCCCCEEEC	19.67	27600695
1124	Phosphorylation	WKHIDDTSQMDLFHR EECCCCCCCCEEECC	30.42	26643407
1133	Phosphorylation	MDLFHRDSDDDQVEE CEEECCCCCCHHHHH	42.78	21149613
1180	Phosphorylation	EEDIGDDSQFMMLAK HHHCCCHHHHHHHHH	30.02	25266776
1199	Phosphorylation	KALQKNASHTVVVQE HHHHHCCCCEEEEEC	29.14	29550500
1263	Phosphorylation	SRNFVFHTLSPTKAE CCCCEEEECCHHHHH	19.91	23984901
1265	Phosphorylation	NFVFHTLSPTKAEAA CCEEEECCHHHHHHH	31.51	26824392
1267	Phosphorylation	VFHTLSPTKAEAAKD EEEECCHHHHHHHHC	39.24	22942356
1286	Phosphorylation	QVRRRGLSSMMSPSP HHHHHCHHHHCCCCC	21.00	26643407
1287	Phosphorylation	VRRRGLSSMMSPSPK HHHHCHHHHCCCCCC	24.44	28066266
1290	Phosphorylation	RGLSSMMSPSPKRLK HCHHHHCCCCCCCCC	17.37	26643407
1292	Phosphorylation	LSSMMSPSPKRLKTN HHHHCCCCCCCCCCC	35.43	22942356
1298	Phosphorylation	PSPKRLKTNGSSPGP CCCCCCCCCCCCCCC	49.78	25777480
1301	Phosphorylation	KRLKTNGSSPGPKRS CCCCCCCCCCCCCHH	35.11	20139300
1302	Phosphorylation	RLKTNGSSPGPKRSI CCCCCCCCCCCCHHH	34.96	25777480

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
893	T	Phosphorylation	Kinase	CHEK1	O35280	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of CLSPN_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CLSPN_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
I5P2_HUMAN	INPP5B	physical	26496610
MCM2_HUMAN	MCM2	physical	26496610
MCM3_HUMAN	MCM3	physical	26496610
MCM4_HUMAN	MCM4	physical	26496610
MCM5_HUMAN	MCM5	physical	26496610
MCM6_HUMAN	MCM6	physical	26496610
MCM7_HUMAN	MCM7	physical	26496610
TIM_HUMAN	TIMELESS	physical	26496610
PNKD_HUMAN	PNKD	physical	26496610
KI26A_HUMAN	KIF26A	physical	26496610
TIPIN_HUMAN	TIPIN	physical	26496610
MMTA2_HUMAN	C1orf35	physical	26496610
RN170_HUMAN	RNF170	physical	26496610
SLD5_HUMAN	GINS4	physical	26496610
TM246_HUMAN	TMEM246	physical	26496610
FNIP1_HUMAN	FNIP1	physical	26496610

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.