dbPTM

CLSPN_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CLSPN_HUMAN
UniProt AC	Q9HAW4
Protein Name	Claspin
Gene Name	CLSPN
Organism	Homo sapiens (Human).
Sequence Length	1339
Subcellular Localization	Nucleus .
Protein Description	Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks..
Protein Sequence	MTGEVGSEVHLEINDPNVISQEEADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETEDTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQENLEAQVKPCLELSLQSGNSTDFTTDRKSSKKHIHDKEGTAGKAKVKSKRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLVDKDLFETGLEDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTTRKERKAARLSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKPRPTCHGNAMALLKSSKYQSSHHKEIIDTANTTEMNSDHHSKGSEQTTGAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRPSPGDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDEKVEEPEQQNKSSAVGPPEKVRRFTLDRLKQLGVDVSIKPRLGADEDSFVILEPETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDMGTDGKEELKADVVPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQALFKLDNEDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEEGNQETAEFLLSSEEIETKDEKEMDKENNDGSSEIGKAVGFLSVPKSLSSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKESSHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSASKSSGKLSEPSLPIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDELLDLCTGKFTSQAEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGDPMEEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGNDLALEDHEDDDEEELLKRSEKLKRQMRLRKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARWRKERIEREQWLRDMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQKNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPKAVLQKLAALSDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKTDDSTSGLTRSIFKYLES

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
26	Phosphorylation	ISQEEADSPSDSGQG CCHHHCCCCCCCCCC	33.09	-
30	Phosphorylation	EADSPSDSGQGSYET HCCCCCCCCCCCCEE	37.04	22817900
34	Phosphorylation	PSDSGQGSYETIGPL CCCCCCCCCEEECCC	16.35	22817900
42	Phosphorylation	YETIGPLSEGDSDEE CEEECCCCCCCCCCC	42.54	-
46	Phosphorylation	GPLSEGDSDEEIFVS CCCCCCCCCCCEEEE	58.75	-
53	Phosphorylation	SDEEIFVSKKLKNRK CCCCEEEEHHHCCCC	17.21	-
60	Ubiquitination	SKKLKNRKVLQDSDS EHHHCCCCCCCCCCC	57.60	-
65	Phosphorylation	NRKVLQDSDSETEDT CCCCCCCCCCCCCCC	30.03	22167270
67	Phosphorylation	KVLQDSDSETEDTNA CCCCCCCCCCCCCCC	50.82	22167270
69	Phosphorylation	LQDSDSETEDTNASP CCCCCCCCCCCCCCC	43.01	29255136
72	Phosphorylation	SDSETEDTNASPEKT CCCCCCCCCCCCCCC	26.84	30108239
75	Phosphorylation	ETEDTNASPEKTTYD CCCCCCCCCCCCCCC	35.34	25159151
79	Phosphorylation	TNASPEKTTYDSAEE CCCCCCCCCCCCHHH	29.00	29978859
80	Phosphorylation	NASPEKTTYDSAEEE CCCCCCCCCCCHHHH	36.08	29978859
81	Phosphorylation	ASPEKTTYDSAEEEN CCCCCCCCCCHHHHH	17.09	28450419
83	Phosphorylation	PEKTTYDSAEEENKE CCCCCCCCHHHHHHH	27.45	22167270
93	Phosphorylation	EENKENLYAGKNTKI HHHHHHCCCCCCHHH	25.53	19664994
96	Ubiquitination	KENLYAGKNTKIKRI HHHCCCCCCHHHHEE	53.96	-
98	Phosphorylation	NLYAGKNTKIKRIYK HCCCCCCHHHHEEEE	37.32	26074081
104	Phosphorylation	NTKIKRIYKTVADSD CHHHHEEEEHHCCCC	12.49	26074081
105	Ubiquitination	TKIKRIYKTVADSDE HHHHEEEEHHCCCCH	33.87	-
106	Phosphorylation	KIKRIYKTVADSDES HHHEEEEHHCCCCHH	12.17	26074081
110	Phosphorylation	IYKTVADSDESYMEK EEEHHCCCCHHHHHH	33.27	21815630
113	Phosphorylation	TVADSDESYMEKSLY HHCCCCHHHHHHHHH	34.47	25262027
114	Phosphorylation	VADSDESYMEKSLYQ HCCCCHHHHHHHHHH	13.77	29449344
120	Phosphorylation	SYMEKSLYQENLEAQ HHHHHHHHHHCHHHH	22.37	25159151
187	Acetylation	MEKIRQLKKKETKNQ HHHHHHHHHHCCCCC	54.16	18603821
188	Acetylation	EKIRQLKKKETKNQE HHHHHHHHHCCCCCC	65.52	18603829
192	Acetylation	QLKKKETKNQEDDVE HHHHHCCCCCCCCCC	58.69	18603837
205	Phosphorylation	VEQPFNDSGCLLVDK CCCCCCCCCEEEEEH	32.29	28464451
217	Phosphorylation	VDKDLFETGLEDENN EEHHHHHHCCCCCCC	39.47	23663014
225	Phosphorylation	GLEDENNSPLEDEES CCCCCCCCCCCCHHH	42.30	29255136
232	Phosphorylation	SPLEDEESLESIRAA CCCCCHHHHHHHHHH	35.08	26074081
235	Phosphorylation	EDEESLESIRAAVKN CCHHHHHHHHHHHHH	23.75	26074081
253	Phosphorylation	KHKKKEPSLESGVHS HCCCCCCCHHHCCCC	45.95	23401153
256	Phosphorylation	KKEPSLESGVHSFEE CCCCCHHHCCCCHHH	51.95	23186163
260	Phosphorylation	SLESGVHSFEEGSEL CHHHCCCCHHHCCCC	31.76	30576142
265	Phosphorylation	VHSFEEGSELSKGTT CCCHHHCCCCCCCCC	38.35	29449344
268	Phosphorylation	FEEGSELSKGTTRKE HHHCCCCCCCCCHHH	25.61	23186163
282	Phosphorylation	ERKAARLSKEALKQL HHHHHHHCHHHHHHH	23.93	23186163
283	Ubiquitination	RKAARLSKEALKQLH HHHHHHCHHHHHHHH	52.15	29967540
287	Ubiquitination	RLSKEALKQLHSETQ HHCHHHHHHHHHHHH	58.84	29967540
306	Phosphorylation	ESALNLPYHMPENKT HHHHCCCCCCCCCCC	17.57	22817900
319	Ubiquitination	KTIHDFFKRKPRPTC CCHHHHHHCCCCCCC	60.55	-
341	Phosphorylation	LKSSKYQSSHHKEII HHCCCCCCCCCHHHH	28.66	28985074
342	Phosphorylation	KSSKYQSSHHKEIID HCCCCCCCCCHHHHH	17.68	28985074
353	Phosphorylation	EIIDTANTTEMNSDH HHHHCCCCCCCCCCC	22.97	28985074
358	Phosphorylation	ANTTEMNSDHHSKGS CCCCCCCCCCCCCCC	36.69	29449344
362	Phosphorylation	EMNSDHHSKGSEQTT CCCCCCCCCCCCCCC	34.98	29449344
387	Phosphorylation	LPVVSKETQIITGSD CCCCCCCEEEECCCC	28.89	20068231
391	Phosphorylation	SKETQIITGSDESCR CCCEEEECCCCHHHH	32.06	20068231
393	Phosphorylation	ETQIITGSDESCRKD CEEEECCCCHHHHHH	29.56	20068231
396	Phosphorylation	IITGSDESCRKDLVK EECCCCHHHHHHHHH	24.98	20068231
403	Sumoylation	SCRKDLVKNEELEIQ HHHHHHHHCHHHHHH	66.97	-
403	Sumoylation	SCRKDLVKNEELEIQ HHHHHHHHCHHHHHH	66.97	-
412	Ubiquitination	EELEIQEKQKQSDIR HHHHHHHHHHHCCCC	46.55	-
414	Ubiquitination	LEIQEKQKQSDIRPS HHHHHHHHHCCCCCC	63.94	-
421	Phosphorylation	KQSDIRPSPGDSSVL HHCCCCCCCCCHHHH	31.50	26074081
425	Phosphorylation	IRPSPGDSSVLQQES CCCCCCCHHHHHHHH	28.41	26074081
426	Phosphorylation	RPSPGDSSVLQQESN CCCCCCHHHHHHHHC	31.42	26074081
480	Phosphorylation	EPEQQNKSSAVGPPE CHHHHCCCCCCCCHH	30.95	23401153
493	Phosphorylation	PEKVRRFTLDRLKQL HHHHHHHHHHHHHHH	26.41	23882029
507	Ubiquitination	LGVDVSIKPRLGADE HCCCEEECCCCCCCC	19.31	-
516	Phosphorylation	RLGADEDSFVILEPE CCCCCCCCEEEECCC	21.26	30266825
532	Ubiquitination	NRELEALKQRFWKHA HHHHHHHHHHHHHHC	46.61	29967540
589	Ubiquitination	LDGASHTKPGEKLQV CCCCCCCCCCHHHHH	45.67	30230243
600	Acetylation	KLQVLKAKLQEAMKL HHHHHHHHHHHHHHH	49.46	25953088
606	Acetylation	AKLQEAMKLRRFEER HHHHHHHHHHHHHHH	45.98	23749302
639	Phosphorylation	EEEEEEMTDESEEDG HHHHHHCCCCCCHHH	39.64	-
642	Phosphorylation	EEEMTDESEEDGEEK HHHCCCCCCHHHHHH	49.44	-
644	Ubiquitination	EMTDESEEDGEEKVE HCCCCCCHHHHHHHH	79.27	29967540
665	Ubiquitination	ELEEEEEKEEEEEEE HHHHHHHHHHHHHHH	73.95	22817900
670	Ubiquitination	EEKEEEEEEEGNQET HHHHHHHHHHHHHHH	65.71	22817900
670 (in isoform 2)	Ubiquitination	-	65.71	21906983
675	Ubiquitination	EEEEEGNQETAEFLL HHHHHHHHHHHHHHH	61.34	22817900
680	Ubiquitination	GNQETAEFLLSSEEI HHHHHHHHHHCHHHH	8.46	22817900
703	Phosphorylation	DKENNDGSSEIGKAV HHCCCCCCCHHHHHH	27.88	23663014
704	Phosphorylation	KENNDGSSEIGKAVG HCCCCCCCHHHHHHH	38.47	23663014
708	Ubiquitination	DGSSEIGKAVGFLSV CCCCHHHHHHHEEEC	45.63	29967540
714	Phosphorylation	GKAVGFLSVPKSLSS HHHHHEEECCCCCCC	33.83	23312004
718	Phosphorylation	GFLSVPKSLSSDSTL HEEECCCCCCCCCEE	27.67	22115753
720	Phosphorylation	LSVPKSLSSDSTLLL EECCCCCCCCCEEEE	38.91	25159151
721	Phosphorylation	SVPKSLSSDSTLLLF ECCCCCCCCCEEEEE	40.65	23898821
723	Phosphorylation	PKSLSSDSTLLLFKD CCCCCCCCEEEEEEC	23.93	25159151
724	Phosphorylation	KSLSSDSTLLLFKDS CCCCCCCEEEEEECC	26.81	23898821
725	Ubiquitination	SLSSDSTLLLFKDSS CCCCCCEEEEEECCC	4.53	22817900
729	Ubiquitination	DSTLLLFKDSSSKMG CCEEEEEECCCCCCC	57.58	22817900
730	Ubiquitination	STLLLFKDSSSKMGY CEEEEEECCCCCCCC	46.55	22817900
731	Phosphorylation	TLLLFKDSSSKMGYF EEEEEECCCCCCCCC	36.78	27732954
732	Phosphorylation	LLLFKDSSSKMGYFP EEEEECCCCCCCCCC	44.08	24719451
733	Phosphorylation	LLFKDSSSKMGYFPT EEEECCCCCCCCCCC	30.52	27732954
734	Ubiquitination	LFKDSSSKMGYFPTE EEECCCCCCCCCCCC	37.99	22817900
734 (in isoform 1)	Ubiquitination	-	37.99	21906983
734 (in isoform 3)	Ubiquitination	-	37.99	21906983
737	Phosphorylation	DSSSKMGYFPTEEKS CCCCCCCCCCCCCCC	11.68	27732954
740	Phosphorylation	SKMGYFPTEEKSETD CCCCCCCCCCCCCCC	47.05	23186163
744	Phosphorylation	YFPTEEKSETDENSG CCCCCCCCCCCCCCC	49.35	28985074
746	Phosphorylation	PTEEKSETDENSGKQ CCCCCCCCCCCCCCC	56.56	28985074
750	Phosphorylation	KSETDENSGKQPSKL CCCCCCCCCCCCCCC	45.37	29978859
752	Acetylation	ETDENSGKQPSKLDE CCCCCCCCCCCCCCC	59.88	26051181
755	Phosphorylation	ENSGKQPSKLDEDDS CCCCCCCCCCCCCCC	43.27	30576142
756	Acetylation	NSGKQPSKLDEDDSC CCCCCCCCCCCCCCC	67.64	7704515
760	Ubiquitination	QPSKLDEDDSCSLLT CCCCCCCCCCCCCCC	53.44	29967540
762	Phosphorylation	SKLDEDDSCSLLTKE CCCCCCCCCCCCCCC	20.23	29255136
764	Phosphorylation	LDEDDSCSLLTKESS CCCCCCCCCCCCCCC	30.30	29255136
764	Ubiquitination	LDEDDSCSLLTKESS CCCCCCCCCCCCCCC	30.30	29967540
767	Phosphorylation	DDSCSLLTKESSHNS CCCCCCCCCCCCCCC	37.75	29255136
770	Phosphorylation	CSLLTKESSHNSSFE CCCCCCCCCCCCCEE	38.15	28464451
771	Phosphorylation	SLLTKESSHNSSFEL CCCCCCCCCCCCEEE	27.98	28464451
774	Phosphorylation	TKESSHNSSFELIGS CCCCCCCCCEEECCC	30.86	22617229
775	Phosphorylation	KESSHNSSFELIGST CCCCCCCCEEECCCC	28.23	22617229
781	Phosphorylation	SSFELIGSTIPSYQP CCEEECCCCCCCCCC	19.03	30576142
782	Phosphorylation	SFELIGSTIPSYQPC CEEECCCCCCCCCCC	31.52	28450419
785	Phosphorylation	LIGSTIPSYQPCNRQ ECCCCCCCCCCCCCC	32.33	26552605
797	O-linked_Glycosylation	NRQTGRGTSFFPTAG CCCCCCCCCCCCCCC	22.47	30379171
797	Phosphorylation	NRQTGRGTSFFPTAG CCCCCCCCCCCCCCC	22.47	20068231
798	Phosphorylation	RQTGRGTSFFPTAGG CCCCCCCCCCCCCCC	27.93	20068231
802	Phosphorylation	RGTSFFPTAGGFRSP CCCCCCCCCCCCCCC	32.55	28102081
808	Phosphorylation	PTAGGFRSPSPGLFR CCCCCCCCCCCCCHH	27.71	30266825
810	Phosphorylation	AGGFRSPSPGLFRAS CCCCCCCCCCCHHHH	32.61	28176443
820	Phosphorylation	LFRASLVSSASKSSG CHHHHHHHHCCCCCC	26.02	23403867
821	Phosphorylation	FRASLVSSASKSSGK HHHHHHHHCCCCCCC	29.14	29255136
823	Phosphorylation	ASLVSSASKSSGKLS HHHHHHCCCCCCCCC	34.71	29255136
824	Ubiquitination	SLVSSASKSSGKLSE HHHHHCCCCCCCCCC	48.64	29967540
825	Phosphorylation	LVSSASKSSGKLSEP HHHHCCCCCCCCCCC	41.65	23403867
826	Phosphorylation	VSSASKSSGKLSEPS HHHCCCCCCCCCCCC	43.62	23403867
827	Acetylation	SSASKSSGKLSEPSL HHCCCCCCCCCCCCC	41.85	19608861
827	Ubiquitination	SSASKSSGKLSEPSL HHCCCCCCCCCCCCC	41.85	29967540
828	Acetylation	SASKSSGKLSEPSLP HCCCCCCCCCCCCCC	51.27	25953088
828	Ubiquitination	SASKSSGKLSEPSLP HCCCCCCCCCCCCCC	51.27	29967540
830	Phosphorylation	SKSSGKLSEPSLPIE CCCCCCCCCCCCCCC	51.45	22167270
833	Phosphorylation	SGKLSEPSLPIEDSQ CCCCCCCCCCCCCCH	42.60	17525332
839	Phosphorylation	PSLPIEDSQDLYNAS CCCCCCCCHHHHHCC	16.89	17525332
843	Phosphorylation	IEDSQDLYNASPEPK CCCCHHHHHCCCCCC	19.57	22167270
846	Phosphorylation	SQDLYNASPEPKTLF CHHHHHCCCCCCEEE	26.59	17525332
851	Phosphorylation	NASPEPKTLFLGAGD HCCCCCCEEEECCCC	33.20	26074081
861	Acetylation	LGAGDFQFCLEDDTQ ECCCCCEEEECCCCH	5.32	19608861
861	Ubiquitination	LGAGDFQFCLEDDTQ ECCCCCEEEECCCCH	5.32	29967540
883	Ubiquitination	GFLNVRNHRNQYQAL CCEECCCCHHHHHHH	21.36	29967540
887	Phosphorylation	VRNHRNQYQALKPRL CCCCHHHHHHHCCCC	10.17	28796482
891	Acetylation	RNQYQALKPRLPLAS HHHHHHHCCCCCCCC	30.98	19608861
891	Ubiquitination	RNQYQALKPRLPLAS HHHHHHHCCCCCCCC	30.98	19608861
899	Ubiquitination	PRLPLASMDENAMDA CCCCCCCCCCCCCCC	6.61	22817900
899 (in isoform 2)	Ubiquitination	-	6.61	21906983
902	Ubiquitination	PLASMDENAMDANMD CCCCCCCCCCCCCHH	35.98	22817900
909	Ubiquitination	NAMDANMDELLDLCT CCCCCCHHHHHHHHC	43.10	22817900
912	Ubiquitination	DANMDELLDLCTGKF CCCHHHHHHHHCCCC	4.43	22817900
916	Phosphorylation	DELLDLCTGKFTSQA HHHHHHHCCCCCHHH	51.18	19828454
921	Phosphorylation	LCTGKFTSQAEKHLP HHCCCCCHHHHHHCC	30.44	23312004
925	Acetylation	KFTSQAEKHLPRKSD CCCHHHHHHCCCCCC	54.15	23749302
925	Ubiquitination	KFTSQAEKHLPRKSD CCCHHHHHHCCCCCC	54.15	19608861
945	Phosphorylation	EELLNLCSGKFTSQD HHHHHHHCCCCCCCC	48.46	21815630
947	Ubiquitination	LLNLCSGKFTSQDAS HHHHHCCCCCCCCCC	29.22	29967540
949	Phosphorylation	NLCSGKFTSQDASTP HHHCCCCCCCCCCCC	29.03	29255136
950	Phosphorylation	LCSGKFTSQDASTPA HHCCCCCCCCCCCCC	29.24	23401153
954	Phosphorylation	KFTSQDASTPASSEL CCCCCCCCCCCCHHH	43.12	30266825
955	Phosphorylation	FTSQDASTPASSELN CCCCCCCCCCCHHHH	25.29	30266825
958	Phosphorylation	QDASTPASSELNKQE CCCCCCCCHHHHHHH	26.08	29255136
959	Phosphorylation	DASTPASSELNKQEK CCCCCCCHHHHHHHH	48.48	29255136
959	Ubiquitination	DASTPASSELNKQEK CCCCCCCHHHHHHHH	48.48	22817900
962	Ubiquitination	TPASSELNKQEKESS CCCCHHHHHHHHHHH	39.65	22817900
963	Ubiquitination	PASSELNKQEKESSM CCCHHHHHHHHHHHC	72.89	22817900
963 (in isoform 1)	Ubiquitination	-	72.89	21906983
963 (in isoform 3)	Ubiquitination	-	72.89	21906983
966	Ubiquitination	SELNKQEKESSMGDP HHHHHHHHHHHCCCH	61.47	22817900
968	Phosphorylation	LNKQEKESSMGDPME HHHHHHHHHCCCHHH	36.37	26074081
969	Phosphorylation	NKQEKESSMGDPMEE HHHHHHHHCCCHHHH	28.07	26074081
982	Phosphorylation	EEALALCSGSFPTDK HHHHHHHHCCCCCCC	38.02	-
987	Phosphorylation	LCSGSFPTDKEEEDE HHHCCCCCCCCCCHH	58.66	-
1005	Phosphorylation	FGDFRLVSNDNEFDS HCCEEEECCCCCCCC	42.95	28176443
1012	Phosphorylation	SNDNEFDSDEDEHSD CCCCCCCCCCCCCCC	48.50	28176443
1018	Phosphorylation	DSDEDEHSDSGNDLA CCCCCCCCCCCCCCC	31.27	28176443
1020	Phosphorylation	DEDEHSDSGNDLALE CCCCCCCCCCCCCCC	42.38	28176443
1096	Phosphorylation	PSDEELQSQIKKIHM CCHHHHHHHHHHHHH	46.64	26074081
1105	Phosphorylation	IKKIHMKTMLDDDKR HHHHHHHHHCCCHHH	18.52	26074081
1117	Phosphorylation	DKRQLRLYQERYLAD HHHHHHHHHHHHCCC	11.24	26074081
1129	Phosphorylation	LADGDLHSDGPGRMR CCCCCCCCCCCCCCC	52.11	30576142
1147	Phosphorylation	WKNIDDASQMDLFHR EECCCCHHHHCCCCC	32.79	29255136
1156	Phosphorylation	MDLFHRDSDDDQTEE HCCCCCCCCCHHHHH	42.78	30266825
1161	Phosphorylation	RDSDDDQTEEQLDES CCCCCHHHHHHHHHH	48.78	30266825
1168	Phosphorylation	TEEQLDESEARWRKE HHHHHHHHHHHHHHH	35.90	26074081
1201	Ubiquitination	AEEEEEIGEDSQFMI HHHHHHHCCCCHHHH	35.62	29967540
1204	Phosphorylation	EEEIGEDSQFMILAK HHHHCCCCHHHHHHH	22.48	30576142
1223	Phosphorylation	KALQKNASRPMVIQE HHHHHCCCCCCHHHC	46.43	29978859
1225	Phosphorylation	LQKNASRPMVIQESK HHHCCCCCCHHHCCH	20.92	32142685
1231	Phosphorylation	RPMVIQESKSLLRNP CCCHHHCCHHHHCCC	15.92	27174698
1233	Phosphorylation	MVIQESKSLLRNPFE CHHHCCHHHHCCCCH	41.75	24719451
1246	Phosphorylation	FEAIRPGSAQQVKTG CHHCCCCCCHHHCCC	26.11	30001349
1252	Phosphorylation	GSAQQVKTGSLLNQP CCCHHHCCCCCCCCH	32.90	32142685
1257	Ubiquitination	VKTGSLLNQPKAVLQ HCCCCCCCCHHHHHH	62.49	29967540
1265	Ubiquitination	QPKAVLQKLAALSDH CHHHHHHHHHHHCCC	35.61	29967540
1280	Phosphorylation	NPSAPRNSRNFVFHT CCCCCCCCCCCEEEE	29.85	28555341
1287	Phosphorylation	SRNFVFHTLSPVKAE CCCCEEEECCHHHHH	19.91	22167270
1289	Phosphorylation	NFVFHTLSPVKAEAA CCEEEECCHHHHHHH	28.89	29255136
1289 (in isoform 3)	Phosphorylation	-	28.89	27762562
1292	Sumoylation	FHTLSPVKAEAAKES EEECCHHHHHHHHHH	43.84	-
1292	Sumoylation	FHTLSPVKAEAAKES EEECCHHHHHHHHHH	43.84	-
1299	Phosphorylation	KAEAAKESSKSQVKK HHHHHHHHCHHHHHH	42.22	29083192
1300	Phosphorylation	AEAAKESSKSQVKKR HHHHHHHCHHHHHHH	37.10	29083192
1302	Phosphorylation	AAKESSKSQVKKRGP HHHHHCHHHHHHHCC	42.00	29083192
1310	Phosphorylation	QVKKRGPSFMTSPSP HHHHHCCCCCCCCCC	30.40	29514088
1313	Phosphorylation	KRGPSFMTSPSPKHL HHCCCCCCCCCCCCC	35.16	29255136
1314	Phosphorylation	RGPSFMTSPSPKHLK HCCCCCCCCCCCCCC	16.16	29255136
1316	Phosphorylation	PSFMTSPSPKHLKTD CCCCCCCCCCCCCCC	46.47	29255136
1321	Sumoylation	SPSPKHLKTDDSTSG CCCCCCCCCCCCCCC	50.02	-
1321	Sumoylation	SPSPKHLKTDDSTSG CCCCCCCCCCCCCCC	50.02	-
1321	Ubiquitination	SPSPKHLKTDDSTSG CCCCCCCCCCCCCCC	50.02	29967540
1322	Phosphorylation	PSPKHLKTDDSTSGL CCCCCCCCCCCCCCH	51.93	29449344
1325	Phosphorylation	KHLKTDDSTSGLTRS CCCCCCCCCCCHHHH	27.55	21815630
1326	Phosphorylation	HLKTDDSTSGLTRSI CCCCCCCCCCHHHHH	33.53	29449344
1327	Phosphorylation	LKTDDSTSGLTRSIF CCCCCCCCCHHHHHH	35.44	29449344
1330	Phosphorylation	DDSTSGLTRSIFKYL CCCCCCHHHHHHHHH	26.37	29449344
1332	Phosphorylation	STSGLTRSIFKYLES CCCCHHHHHHHHHHC	27.43	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
30	S	Phosphorylation	Kinase	PLK1	P53350	PSP
67	S	Phosphorylation	Kinase	CDC7	O00311	PSP
110	S	Phosphorylation	Kinase	CDC7	O00311	PSP
260	S	Phosphorylation	Kinase	CDC7	O00311	PSP
265	S	Phosphorylation	Kinase	CDC7	O00311	PSP
704	S	Phosphorylation	Kinase	CDC7	O00311	PSP
721	S	Phosphorylation	Kinase	CDC7	O00311	PSP
744	S	Phosphorylation	Kinase	CDC7	O00311	PSP
916	T	Phosphorylation	Kinase	CHK1	O14757	PSP
1129	S	Phosphorylation	Kinase	CDC7	O00311	PSP
1147	S	Phosphorylation	Kinase	CDC7	O00311	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	FZR1	Q9UM11	PMID:18662541
-	K	Ubiquitination	E3 ubiquitin ligase	BRCA1	P38398	PMID:22863316
-	K	Ubiquitination	E3 ubiquitin ligase	FBXW11	Q9UKB1	PMID:24658274
-	K	Ubiquitination	E3 ubiquitin ligase	BTRC	Q9Y297	PMID:24658274

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of CLSPN_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CLSPN_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BRCA1_HUMAN	BRCA1	physical	15096610
PCNA_HUMAN	PCNA	physical	18451105
TIM_HUMAN	TIMELESS	physical	18451105
PLK1_HUMAN	PLK1	physical	16885022
PMS2_HUMAN	PMS2	physical	20029092
MSH6_HUMAN	MSH6	physical	20029092
TNFB_HUMAN	LTA	physical	18922873
CHK1_HUMAN	CHEK1	physical	16963448
RAD17_HUMAN	RAD17	physical	16885023
FBW1A_HUMAN	BTRC	physical	16885022
CDC7_HUMAN	CDC7	physical	18084324
HERC2_HUMAN	HERC2	physical	21775519
CHK1_HUMAN	CHEK1	physical	16123041
UBP20_HUMAN	USP20	physical	25355518
UBP20_HUMAN	USP20	physical	25326330

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CLSPN_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-891 AND LYS-925, AND MASSSPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-839; SER-846; SER-1156AND SER-1289, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-67; SER-723;SER-810; SER-846; SER-950; SER-954; THR-955 AND SER-959, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-67; SER-83;SER-225; SER-720; THR-724; SER-808; SER-810; SER-846; SER-1012;SER-1018; SER-1020; SER-1156 AND SER-1289, AND MASS SPECTROMETRY.	18669648 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-833; SER-839; SER-846AND SER-950, AND MASS SPECTROMETRY.	17525332 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-67; SER-75;SER-762; SER-764; THR-1287 AND SER-1289, AND MASS SPECTROMETRY.	17081983 [Abstract]
"Repeated phosphopeptide motifs in human Claspin are phosphorylated byChk1 and mediate Claspin function."; Chini C.C., Chen J.; J. Biol. Chem. 281:33276-33282(2006). Cited for: PHOSPHORYLATION AT THR-916 BY CHEK1, AND INTERACTION WITH CHEK1.	16963448 [Abstract]