dbPTM

CLAP2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CLAP2_MOUSE
UniProt AC	Q8BRT1
Protein Name	CLIP-associating protein 2
Gene Name	Clasp2
Organism	Mus musculus (Mouse).
Sequence Length	1286
Subcellular Localization	Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Golgi apparatus . Golgi apparatus, trans-Golgi netw
Protein Description	Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex..
Protein Sequence	MRRLICKRICDYKSFDDEESVDGNRPSSAASAFKVPAPKTPGNPVSSARKPGSAGGPKVGGPSKEGGAGAVDEDDFIKAFTDVPSVQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGRVSVGGSKANPLPGSLQRSRSDIDVNAAAGAKAHHAAGQAVRSGRLGAGALNPGSYASLEDTSDKMDGTASDDGRVRAKLSTPLVAVGNAKTDSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGAQRVLVNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALLLGDIRTKKKPARRRYESYGMHSDDDANSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRIELKRLCEIFTRMFADPHGKVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDITRESFPNDLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPRDFTNSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMSEPVRRDPKKEDGDTICSGPGMSDPRAGGDAADGSQPALDNKASLLHSMPLHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSDLVAELLKELSNHNERIEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLKEILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAAIKMQTKVIERVSKETLNMLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMKLLNLYIKRAQTGSAGADPTADVSGQS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
8 (in isoform 6)	Phosphorylation	-	30.29	27742792
12	Phosphorylation	ICKRICDYKSFDDEE HHHHHCCCCCCCCHH	12.24	29899451
14	Phosphorylation	KRICDYKSFDDEESV HHHCCCCCCCCHHCC	27.90	25521595
14 (in isoform 6)	Phosphorylation	-	27.90	29899451
20	Phosphorylation	KSFDDEESVDGNRPS CCCCCHHCCCCCCCC	24.38	25521595
21 (in isoform 6)	Phosphorylation	-	14.29	25338131
27	Phosphorylation	SVDGNRPSSAASAFK CCCCCCCCCCHHHCC	29.40	30352176
28	Phosphorylation	VDGNRPSSAASAFKV CCCCCCCCCHHHCCC	30.45	25521595
40	Phosphorylation	FKVPAPKTPGNPVSS CCCCCCCCCCCCCCC	35.06	21183079
46	Phosphorylation	KTPGNPVSSARKPGS CCCCCCCCCCCCCCC	21.38	22871156
47	Phosphorylation	TPGNPVSSARKPGSA CCCCCCCCCCCCCCC	32.12	21183079
53	Phosphorylation	SSARKPGSAGGPKVG CCCCCCCCCCCCCCC	32.06	21183079
93 (in isoform 5)	Phosphorylation	-	63.58	22871156
156	Phosphorylation	LSAKDLRSQVVREAC CCHHHHHHHHHHHHH	34.32	-
170	Phosphorylation	CITVAHLSTVLGNKF HHHHHHHHHHHCCCC	13.45	-
171	Phosphorylation	ITVAHLSTVLGNKFD HHHHHHHHHHCCCCC	26.55	-
229 (in isoform 5)	Phosphorylation	-	25.74	25338131
235 (in isoform 5)	Phosphorylation	-	23.40	25338131
241 (in isoform 5)	Phosphorylation	-	6.40	26824392
247 (in isoform 5)	Phosphorylation	-	50.26	28464351
254 (in isoform 5)	Phosphorylation	-	5.35	25338131
282	Acetylation	EARVEARKTYMGLRN HHHHHHHHHHHHHHH	51.56	7714147
309	Phosphorylation	LEPSYQKSLQTYLKS CCHHHHHHHHHHHHH	15.35	25293948
312	Phosphorylation	SYQKSLQTYLKSSGS HHHHHHHHHHHHCCC	35.79	25293948
313	Phosphorylation	YQKSLQTYLKSSGSV HHHHHHHHHHHCCCC	10.12	25293948
316	Phosphorylation	SLQTYLKSSGSVASL HHHHHHHHCCCCCCC	37.02	30635358
317	Phosphorylation	LQTYLKSSGSVASLP HHHHHHHCCCCCCCC	33.24	19060867
319	Phosphorylation	TYLKSSGSVASLPQS HHHHHCCCCCCCCCC	19.23	25521595
322	Phosphorylation	KSSGSVASLPQSDRS HHCCCCCCCCCCCCC	38.13	30635358
326	Phosphorylation	SVASLPQSDRSSSSS CCCCCCCCCCCCCCC	32.81	21454597
329	Phosphorylation	SLPQSDRSSSSSQES CCCCCCCCCCCCHHH	39.29	21082442
330	Phosphorylation	LPQSDRSSSSSQESL CCCCCCCCCCCHHHH	34.82	25619855
331	Phosphorylation	PQSDRSSSSSQESLN CCCCCCCCCCHHHHC	34.94	27742792
332	Phosphorylation	QSDRSSSSSQESLNR CCCCCCCCCHHHHCC	37.68	25521595
333	Phosphorylation	SDRSSSSSQESLNRP CCCCCCCCHHHHCCC	39.66	27742792
336	Phosphorylation	SSSSSQESLNRPFSS CCCCCHHHHCCCCCC	24.25	27742792
342	Phosphorylation	ESLNRPFSSKWSTAN HHHCCCCCCCCCCCC	33.42	25619855
343	Phosphorylation	SLNRPFSSKWSTANP HHCCCCCCCCCCCCC	38.22	25619855
358	Phosphorylation	STVAGRVSVGGSKAN CCCCEEEECCCCCCC	17.43	29514104
370	Phosphorylation	KANPLPGSLQRSRSD CCCCCCCCCCCCCCC	21.27	26824392
374	Phosphorylation	LPGSLQRSRSDIDVN CCCCCCCCCCCCCHH	24.15	24925903
376	Phosphorylation	GSLQRSRSDIDVNAA CCCCCCCCCCCHHHH	39.92	24925903
410	Phosphorylation	AGALNPGSYASLEDT CCCCCCCCCCCCCCC	20.84	25521595
411	Phosphorylation	GALNPGSYASLEDTS CCCCCCCCCCCCCCC	13.21	25521595
413	Phosphorylation	LNPGSYASLEDTSDK CCCCCCCCCCCCCCC	24.86	25521595
418	Phosphorylation	YASLEDTSDKMDGTA CCCCCCCCCCCCCCC	47.78	-
424	Phosphorylation	TSDKMDGTASDDGRV CCCCCCCCCCCCCCE	20.80	29899451
426	Phosphorylation	DKMDGTASDDGRVRA CCCCCCCCCCCCEEE	35.75	22807455
436	Phosphorylation	GRVRAKLSTPLVAVG CCEEEEECCCEEEEC	27.54	25266776
459	O-linked_Glycosylation	RSRTKMVSQSQPGSR CCCCEEEECCCCCCC	22.18	8389769
459	Phosphorylation	RSRTKMVSQSQPGSR CCCCEEEECCCCCCC	22.18	23684622
461	Phosphorylation	RTKMVSQSQPGSRSG CCEEEECCCCCCCCC	30.73	25521595
465	Phosphorylation	VSQSQPGSRSGSPGR EECCCCCCCCCCCCC	29.92	25521595
467	Phosphorylation	QSQPGSRSGSPGRVL CCCCCCCCCCCCCEE	45.37	26824392
469	Phosphorylation	QPGSRSGSPGRVLTT CCCCCCCCCCCEEEH	25.98	26824392
475	Phosphorylation	GSPGRVLTTTALSTV CCCCCEEEHHHHHHC	21.17	26160508
476	O-linked_Glycosylation	SPGRVLTTTALSTVS CCCCEEEHHHHHHCC	13.10	22645316
476	Phosphorylation	SPGRVLTTTALSTVS CCCCEEEHHHHHHCC	13.10	26160508
477	Phosphorylation	PGRVLTTTALSTVSS CCCEEEHHHHHHCCC	21.99	26160508
480	Phosphorylation	VLTTTALSTVSSGAQ EEEHHHHHHCCCCCC	25.05	23984901
481	Phosphorylation	LTTTALSTVSSGAQR EEHHHHHHCCCCCCE	25.84	23984901
484	Phosphorylation	TALSTVSSGAQRVLV HHHHHCCCCCCEEEE	33.54	24759943
493	Phosphorylation	AQRVLVNSASAQKRS CCEEEECCHHHHHHC	19.00	26745281
495	Phosphorylation	RVLVNSASAQKRSKI EEEECCHHHHHHCCC	30.93	25266776
505	Phosphorylation	KRSKIPRSQGCSREA HHCCCCHHHCCCCCC	26.10	25266776
509	Phosphorylation	IPRSQGCSREASPSR CCHHHCCCCCCCHHH	39.72	24899341
513	Phosphorylation	QGCSREASPSRLSVA HCCCCCCCHHHHHHH	20.75	26824392
515	Phosphorylation	CSREASPSRLSVARS CCCCCCHHHHHHHHH	43.27	22324799
518	Phosphorylation	EASPSRLSVARSSRI CCCHHHHHHHHHCCC	16.78	22324799
522	Phosphorylation	SRLSVARSSRIPRPS HHHHHHHHCCCCCCC	17.77	19854140
523	Phosphorylation	RLSVARSSRIPRPSV HHHHHHHCCCCCCCH	28.77	19854140
529	Phosphorylation	SSRIPRPSVSQGCSR HCCCCCCCHHHCCCH	35.36	25521595
531	O-linked_Glycosylation	RIPRPSVSQGCSREA CCCCCCHHHCCCHHH	25.78	1919891
531	Phosphorylation	RIPRPSVSQGCSREA CCCCCCHHHCCCHHH	25.78	25521595
535	Phosphorylation	PSVSQGCSREASRES CCHHHCCCHHHHHHC	39.72	25521595
539	Phosphorylation	QGCSREASRESSRDT HCCCHHHHHHCCCCC	31.65	25266776
542	Phosphorylation	SREASRESSRDTSPV CHHHHHHCCCCCCCC	29.57	24899341
543	Phosphorylation	REASRESSRDTSPVR HHHHHHCCCCCCCCC	29.44	25521595
546	Phosphorylation	SRESSRDTSPVRSFQ HHHCCCCCCCCCCCC	33.54	25521595
547	Phosphorylation	RESSRDTSPVRSFQP HHCCCCCCCCCCCCC	26.20	25521595
551	Phosphorylation	RDTSPVRSFQPLGPG CCCCCCCCCCCCCCC	28.59	23984901
559	Phosphorylation	FQPLGPGYGISQSSR CCCCCCCCCCCHHHH	17.89	24704852
562	Phosphorylation	LGPGYGISQSSRLSS CCCCCCCCHHHHCCC	21.46	29899451
568	Phosphorylation	ISQSSRLSSSVSAMR CCHHHHCCCCCCHHH	21.32	28833060
569	Phosphorylation	SQSSRLSSSVSAMRV CHHHHCCCCCCHHHH	39.07	28833060
570	Phosphorylation	QSSRLSSSVSAMRVL HHHHCCCCCCHHHHH	19.40	23527152
572	Phosphorylation	SRLSSSVSAMRVLNT HHCCCCCCHHHHHCC	20.49	28833060
579	Phosphorylation	SAMRVLNTGSDVEEA CHHHHHCCCCCHHHH	33.62	20415495
581	Phosphorylation	MRVLNTGSDVEEAVA HHHHCCCCCHHHHHH	36.12	15345747
607	Phosphorylation	KKPARRRYESYGMHS CCCCHHHHHHCCCCC	13.86	29899451
609	Phosphorylation	PARRRYESYGMHSDD CCHHHHHHCCCCCCC	20.32	21149613
610	Phosphorylation	ARRRYESYGMHSDDD CHHHHHHCCCCCCCC	13.41	21149613
614	Phosphorylation	YESYGMHSDDDANSD HHHCCCCCCCCCCCC	33.57	25521595
620	Phosphorylation	HSDDDANSDASSACS CCCCCCCCCHHHHHH	35.95	21149613
624	Phosphorylation	DANSDASSACSERSY CCCCCHHHHHHHHHH	34.71	29899451
627	Phosphorylation	SDASSACSERSYSSR CCHHHHHHHHHHHCC	35.44	29899451
630	Phosphorylation	SSACSERSYSSRNGS HHHHHHHHHHCCCCC	25.89	20531401
633	Phosphorylation	CSERSYSSRNGSIPT HHHHHHHCCCCCCCC	22.87	29899451
637	Phosphorylation	SYSSRNGSIPTYMRQ HHHCCCCCCCCCCCC	29.01	29472430
664	Ubiquitination	SSNWSERKEGLLGLQ CCCHHHHHHHHHHHH	52.67	-
777	Phosphorylation	MRFTVDQTQTPSLKV EEEECCCCCCCCHHH	29.91	28066266
779	Phosphorylation	FTVDQTQTPSLKVKV EECCCCCCCCHHHHH	20.45	28066266
781	Phosphorylation	VDQTQTPSLKVKVAI CCCCCCCCHHHHHHH	44.18	28066266
797	Ubiquitination	KYIETLAKQMDPRDF HHHHHHHHCCCHHHC	49.63	-
880	Phosphorylation	NTGNGTQSSMGSPLT HCCCCCCCCCCCCCC	23.33	29899451
881	Phosphorylation	TGNGTQSSMGSPLTR CCCCCCCCCCCCCCC	19.83	29899451
884	Phosphorylation	GTQSSMGSPLTRPTP CCCCCCCCCCCCCCC	14.22	30482847
893	Phosphorylation	LTRPTPRSPANWSSP CCCCCCCCCCCCCCC	29.57	25293948
898	Phosphorylation	PRSPANWSSPLTSPT CCCCCCCCCCCCCCC	23.14	24224561
899	Phosphorylation	RSPANWSSPLTSPTN CCCCCCCCCCCCCCC	18.62	24224561
902	Phosphorylation	ANWSSPLTSPTNTSQ CCCCCCCCCCCCCCC	35.72	24224561
903	Phosphorylation	NWSSPLTSPTNTSQN CCCCCCCCCCCCCCC	37.09	19060867
905	Phosphorylation	SSPLTSPTNTSQNTL CCCCCCCCCCCCCCC	51.39	19060867
907	Phosphorylation	PLTSPTNTSQNTLSP CCCCCCCCCCCCCCC	33.88	25293948
908	Phosphorylation	LTSPTNTSQNTLSPS CCCCCCCCCCCCCCH	24.41	25293948
911	Phosphorylation	PTNTSQNTLSPSAFD CCCCCCCCCCCHHHC	22.45	25293948
913	Phosphorylation	NTSQNTLSPSAFDYD CCCCCCCCCHHHCCC	18.12	19060867
915	Phosphorylation	SQNTLSPSAFDYDTE CCCCCCCHHHCCCCC	37.89	25293948
919	Phosphorylation	LSPSAFDYDTENMNS CCCHHHCCCCCCCCH	20.50	25293948
921	Phosphorylation	PSAFDYDTENMNSED CHHHCCCCCCCCHHH	24.03	25293948
926	Phosphorylation	YDTENMNSEDIYSSL CCCCCCCHHHHHHHH	26.36	25293948
944	Phosphorylation	TEAIQNFSFRSQEDM HHHHHHCCCCCCCCC	27.57	25266776
947	Phosphorylation	IQNFSFRSQEDMSEP HHHCCCCCCCCCCCC	35.59	25521595
952	Phosphorylation	FRSQEDMSEPVRRDP CCCCCCCCCCCCCCC	51.58	26643407
995	Phosphorylation	PALDNKASLLHSMPL CCCCCHHHHHHCCCC	32.83	20415495
999	Phosphorylation	NKASLLHSMPLHSSP CHHHHHHCCCCCCCC	23.28	22942356
1004	Phosphorylation	LHSMPLHSSPRSRDY HHCCCCCCCCCCCCC	50.12	26824392
1005	Phosphorylation	HSMPLHSSPRSRDYN HCCCCCCCCCCCCCC	17.37	26824392
1008	Phosphorylation	PLHSSPRSRDYNPYN CCCCCCCCCCCCCCC	32.65	23608596
1011	Phosphorylation	SSPRSRDYNPYNYSD CCCCCCCCCCCCCCC	19.54	23608596
1014	Phosphorylation	RSRDYNPYNYSDSIS CCCCCCCCCCCCCCC	24.08	18563927
1017	Phosphorylation	DYNPYNYSDSISPFN CCCCCCCCCCCCCCC	22.65	30635358
1019	Phosphorylation	NPYNYSDSISPFNKS CCCCCCCCCCCCCHH	21.01	21743459
1021	Phosphorylation	YNYSDSISPFNKSAL CCCCCCCCCCCHHHH	27.65	23684622
1026	Phosphorylation	SISPFNKSALKEAMF CCCCCCHHHHHHHCC	39.68	21659604
1045	Phosphorylation	DQFPDDLSLDHSDLV HHCCCCCCCCHHHHH	38.45	29899451
1049	Phosphorylation	DDLSLDHSDLVAELL CCCCCCHHHHHHHHH	32.10	29899451
1171	S-palmitoylation	TSISPEQCIKVLCPI HCCCHHHHHHHHHHH	2.87	28680068
1176	S-palmitoylation	EQCIKVLCPIIQTAD HHHHHHHHHHHHCCC	2.26	28680068
1271	Phosphorylation	LYIKRAQTGSAGADP HHHHHHHCCCCCCCC	31.92	29899451
1273	Phosphorylation	IKRAQTGSAGADPTA HHHHHCCCCCCCCCC	27.24	25521595
1283	Phosphorylation	ADPTADVSGQS---- CCCCCCCCCCC----	31.49	29899451
1286	Phosphorylation	TADVSGQS------- CCCCCCCC-------	45.79	29899451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of CLAP2_MOUSE !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
241	S	Phosphorylation		-
Isoform 2 is phosphorylated on Ser-241.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CLAP2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBIP1_HUMAN	UBP1	physical	26496610
CLAP1_HUMAN	CLASP1	physical	26496610
IBTK_HUMAN	IBTK	physical	26496610
PPHLN_HUMAN	PPHLN1	physical	26496610
ZN598_HUMAN	ZNF598	physical	26496610

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CLAP2_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, AND MASSSPECTROMETRY.	19367708 [Abstract]
"Comprehensive identification of phosphorylation sites in postsynapticdensity preparations."; Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R.,Burlingame A.L.; Mol. Cell. Proteomics 5:914-922(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-513, AND MASSSPECTROMETRY.	16452087 [Abstract]
"Phosphoproteomic analysis of the developing mouse brain."; Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.; Mol. Cell. Proteomics 3:1093-1101(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-581, AND MASSSPECTROMETRY.	15345747 [Abstract]

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