CDC20_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: CDC20_RAT
• UniProt AC: Q62623
• Protein Name: Cell division cycle protein 20 homolog
• Gene Name: Cdc20
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 499
• Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
• Protein Description: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation (By similarity). Interacts with NEK2 (By similarity)..
• Protein Sequence: MAQFVFESDLHSLLQLDAPIPNAPIARWQRKAKEATGPAPSPMRAANRSHSAGRTPGRTPGKSNSKVQTTPSKPGGDRYIPQRSASQMEVASFLLSKENQPEDGGTPTKKEHQKAWARNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKACRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWNAGSGDILQLLQMEQPGDYISSVAWIKEGNYLAVGTSNAEVQLWDVQQQKRLRNMTSHSARVSSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNIVNVWPSGPGESGWVPLQTFTQHQGAVKAVAWCPWQSNILATGGGTSDRHIRIWNVCSGACLSAVDVHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTARVLSLTMSPDGATVASAAADETLRLWRCFELDPALRREREKASTSKSSLIHQGIR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
41	Phosphorylation	EATGPAPSPMRAANR HCCCCCCCHHHHHHH	33.17	28432305
51	Phosphorylation	RAANRSHSAGRTPGR HHHHHCCCCCCCCCC	33.39	21725312
66	Acetylation	TPGKSNSKVQTTPSK CCCCCCCCCCCCCCC	42.48	-
69	Phosphorylation	KSNSKVQTTPSKPGG CCCCCCCCCCCCCCC	43.60	28432305
70	Phosphorylation	SNSKVQTTPSKPGGD CCCCCCCCCCCCCCC	14.42	28432305
72	Phosphorylation	SKVQTTPSKPGGDRY CCCCCCCCCCCCCCC	49.76	28432305
84	Phosphorylation	DRYIPQRSASQMEVA CCCCCCCCHHHHHHH	27.33	23984901
86	Phosphorylation	YIPQRSASQMEVASF CCCCCCHHHHHHHHH	31.16	28432305
92	Phosphorylation	ASQMEVASFLLSKEN HHHHHHHHHHHCCCC	22.92	-
106	Phosphorylation	NQPEDGGTPTKKEHQ CCCCCCCCCCHHHHH	33.06	29779826
153	Phosphorylation	NRLKVLYSQKATPGS HHHHHHHCCCCCCCC	22.50	-
157	Phosphorylation	VLYSQKATPGSSRKA HHHCCCCCCCCCHHH	34.91	-
161	Phosphorylation	QKATPGSSRKACRYI CCCCCCCCHHHHHCC	43.65	-
432	Phosphorylation	LVIWKYPTMAKVAEL EEEEECCCHHHHHHH	27.03	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
51	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
41	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
66	K	Acetylation	Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C) (By similarity).	-
72	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
92	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
153	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
157	T	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
161	S	Phosphorylation	Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.	-
485	K	ubiquitylation	Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex (By similarity).	-
490	K	ubiquitylation	Ubiquitinated at Lys-490 during prometaphase.	-
490	K	ubiquitylation	Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of CDC20_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PLCB1_RAT	Plcb1	physical	19347873
1433E_RAT	Ywhae	physical	19167333
TBG1_RAT	Tubg1	physical	19167333
KCC2B_RAT	Camk2b	physical	21725312

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.