dbPTM

KCC2B_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KCC2B_RAT
UniProt AC	P08413
Protein Name	Calcium/calmodulin-dependent protein kinase type II subunit beta
Gene Name	Camk2b
Organism	Rattus norvegicus (Rat).
Sequence Length	542
Subcellular Localization	Sarcoplasmic reticulum membrane Peripheral membrane protein Cytoplasmic side. Cell junction, synapse . Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with the cortical actin cytoskeleton by
Protein Description	Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2..
Protein Sequence	MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSSAITSPKGSLPPAALEPQTTVIHNPVDGIKESSDSTNTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRPDGKWQNVHFHCSGAPVAPLQ

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
14	Phosphorylation	CTRFTDEYQLYEDIG ECCCCCCCHHHHHHC	13.46	-
17	Phosphorylation	FTDEYQLYEDIGKGA CCCCCHHHHHHCCCH	8.95	-
22	Ubiquitination	QLYEDIGKGAFSVVR HHHHHHCCCHHHHHH	47.61	-
26	Phosphorylation	DIGKGAFSVVRRCVK HHCCCHHHHHHHHHH	21.11	25403869
33	Acetylation	SVVRRCVKLCTGHEY HHHHHHHHHHCCCHH	41.28	25786129
33	Ubiquitination	SVVRRCVKLCTGHEY HHHHHHHHHHCCCHH	41.28	-
40	Phosphorylation	KLCTGHEYAAKIINT HHHCCCHHHHHHHHC	13.24	-
43	Ubiquitination	TGHEYAAKIINTKKL CCCHHHHHHHHCCCC	35.63	-
48	Ubiquitination	AAKIINTKKLSARDH HHHHHHCCCCCHHHH	47.42	-
49	Ubiquitination	AKIINTKKLSARDHQ HHHHHCCCCCHHHHH	45.71	-
57	Acetylation	LSARDHQKLEREARI CCHHHHHHHHHHHHH	49.23	22902405
69	Ubiquitination	ARICRLLKHSNIVRL HHHHHHHHHCCEEEE	49.97	-
69	Acetylation	ARICRLLKHSNIVRL HHHHHHHHHCCEEEE	49.97	22902405
107	Phosphorylation	EDIVAREYYSEADAS HHHHHHHHCCCCCHH	13.51	-
138	Ubiquitination	GVVHRDLKPENLLLA CCCCCCCCHHHHHHH	55.35	-
138	Acetylation	GVVHRDLKPENLLLA CCCCCCCCHHHHHHH	55.35	7767833
146	Phosphorylation	PENLLLASKCKGAAV HHHHHHHHCCCCCCE	38.26	27097102
147	Ubiquitination	ENLLLASKCKGAAVK HHHHHHHCCCCCCEE	34.07	-
149	Ubiquitination	LLLASKCKGAAVKLA HHHHHCCCCCCEEHH	56.15	-
154	Ubiquitination	KCKGAAVKLADFGLA CCCCCCEEHHHCCEE	32.98	-
182	Phosphorylation	AGTPGYLSPEVLRKE CCCCCCCCHHHHHHH	15.55	30181290
221	Methylation	FWDEDQHKLYQQIKA CCCHHHHHHHHHHHH	43.17	12692561
221	"N6,N6-dimethyllysine"	FWDEDQHKLYQQIKA CCCHHHHHHHHHHHH	43.17	-
223	Phosphorylation	DEDQHKLYQQIKAGA CHHHHHHHHHHHHCC	11.81	-
227	Ubiquitination	HKLYQQIKAGAYDFP HHHHHHHHHCCCCCC	36.11	-
227	Acetylation	HKLYQQIKAGAYDFP HHHHHHHHHCCCCCC	36.11	22902405
231	Phosphorylation	QQIKAGAYDFPSPEW HHHHHCCCCCCCCCH	19.73	-
235	Phosphorylation	AGAYDFPSPEWDTVT HCCCCCCCCCHHCCC	35.13	28551015
240	Phosphorylation	FPSPEWDTVTPEAKN CCCCCHHCCCHHHHH	27.61	-
242	Phosphorylation	SPEWDTVTPEAKNLI CCCHHCCCHHHHHHH	19.94	-
246	Ubiquitination	DTVTPEAKNLINQML HCCCHHHHHHHHHHH	51.10	-
254	Phosphorylation	NLINQMLTINPAKRI HHHHHHHCCCHHHCC	17.23	27097102
259	Ubiquitination	MLTINPAKRITAHEA HHCCCHHHCCCHHHH	45.66	-
259	Acetylation	MLTINPAKRITAHEA HHCCCHHHCCCHHHH	45.66	134805
268	Ubiquitination	ITAHEALKHPWVCQR CCHHHHHCCCCHHCH	55.45	-
268	Acetylation	ITAHEALKHPWVCQR CCHHHHHCCCCHHCH	55.45	22902405
276	Phosphorylation	HPWVCQRSTVASMMH CCCHHCHHHHHHHHH	11.01	30411139
277	Phosphorylation	PWVCQRSTVASMMHR CCHHCHHHHHHHHHH	23.45	27097102
280	Phosphorylation	CQRSTVASMMHRQET HCHHHHHHHHHHHHH	16.59	27097102
287	Phosphorylation	SMMHRQETVECLKKF HHHHHHHHHHHHHHH	17.17	8443414
292	Acetylation	QETVECLKKFNARRK HHHHHHHHHHCHHHH	67.96	22902405
292	Ubiquitination	QETVECLKKFNARRK HHHHHHHHHHCHHHH	67.96	-
293	Ubiquitination	ETVECLKKFNARRKL HHHHHHHHHCHHHHH	30.79	-
299	Ubiquitination	KKFNARRKLKGAILT HHHCHHHHHHHHHHH	49.34	-
301	Ubiquitination	FNARRKLKGAILTTM HCHHHHHHHHHHHHH	49.70	-
306	Phosphorylation	KLKGAILTTMLATRN HHHHHHHHHHHHCCC	12.21	28432305
307	Phosphorylation	LKGAILTTMLATRNF HHHHHHHHHHHCCCC	13.52	28432305
311	Phosphorylation	ILTTMLATRNFSVGR HHHHHHHCCCCCCCC	23.37	27097102
315	Phosphorylation	MLATRNFSVGRQTTA HHHCCCCCCCCCCCC	27.49	25403869
320	Phosphorylation	NFSVGRQTTAPATMS CCCCCCCCCCCCCCC	24.46	-
321	Phosphorylation	FSVGRQTTAPATMST CCCCCCCCCCCCCCC	23.47	-
325	Phosphorylation	RQTTAPATMSTAASG CCCCCCCCCCCCCCC	15.59	-
327	Phosphorylation	TTAPATMSTAASGTT CCCCCCCCCCCCCCC	15.56	-
328	Phosphorylation	TAPATMSTAASGTTM CCCCCCCCCCCCCCH	18.54	-
331	Phosphorylation	ATMSTAASGTTMGLV CCCCCCCCCCCHHHH	33.92	-
333	Phosphorylation	MSTAASGTTMGLVEQ CCCCCCCCCHHHHHH	15.89	-
334	Phosphorylation	STAASGTTMGLVEQA CCCCCCCCHHHHHHH	16.87	-
342	Ubiquitination	MGLVEQAKSLLNKKA HHHHHHHHHHHHHCC	41.13	-
343	Phosphorylation	GLVEQAKSLLNKKAD HHHHHHHHHHHHCCC	40.90	30181290
347	Ubiquitination	QAKSLLNKKADGVKP HHHHHHHHCCCCCCC	49.67	-
348	Ubiquitination	AKSLLNKKADGVKPQ HHHHHHHCCCCCCCC	51.11	-
353	Ubiquitination	NKKADGVKPQTNSTK HHCCCCCCCCCCCCC	36.62	-
356	Phosphorylation	ADGVKPQTNSTKNSS CCCCCCCCCCCCCCC	39.40	28432305
358	Phosphorylation	GVKPQTNSTKNSSAI CCCCCCCCCCCCCCC	44.11	27097102
359	Phosphorylation	VKPQTNSTKNSSAIT CCCCCCCCCCCCCCC	35.99	28432305
360	Ubiquitination	KPQTNSTKNSSAITS CCCCCCCCCCCCCCC	55.44	-
362	Phosphorylation	QTNSTKNSSAITSPK CCCCCCCCCCCCCCC	23.78	28432305
363	Phosphorylation	TNSTKNSSAITSPKG CCCCCCCCCCCCCCC	32.84	27097102
366	Phosphorylation	TKNSSAITSPKGSLP CCCCCCCCCCCCCCC	38.40	27097102
367	Phosphorylation	KNSSAITSPKGSLPP CCCCCCCCCCCCCCH	20.64	30411139
369	Ubiquitination	SSAITSPKGSLPPAA CCCCCCCCCCCCHHH	61.98	-
371	Phosphorylation	AITSPKGSLPPAALE CCCCCCCCCCHHHCC	43.77	25403869
381	Phosphorylation	PAALEPQTTVIHNPV HHHCCCCCEEEECCC	33.47	22673903
382	Phosphorylation	AALEPQTTVIHNPVD HHCCCCCEEEECCCC	16.53	22673903
394	Phosphorylation	PVDGIKESSDSTNTT CCCCCCCCCCCCCCC	34.15	25403869
395	Phosphorylation	VDGIKESSDSTNTTI CCCCCCCCCCCCCCC	36.35	30240740
397	Phosphorylation	GIKESSDSTNTTIED CCCCCCCCCCCCCCH	26.04	30411139
398	Phosphorylation	IKESSDSTNTTIEDE CCCCCCCCCCCCCHH	41.39	25403869
400	Phosphorylation	ESSDSTNTTIEDEDA CCCCCCCCCCCHHHH	29.28	25403869
401	Phosphorylation	SSDSTNTTIEDEDAK CCCCCCCCCCHHHHH	25.16	30240740
411	Ubiquitination	DEDAKARKQEIIKTT HHHHHHHHHHHHHHH	58.08	-
416	Ubiquitination	ARKQEIIKTTEQLIE HHHHHHHHHHHHHHH	55.87	-
469	Ubiquitination	YFENLLAKNSKPIHT HHHHHHHCCCCCCEE	62.82	-
472	Ubiquitination	NLLAKNSKPIHTTIL HHHHCCCCCCEEEEC	58.34	-
501	Phosphorylation	AYIRLTQYIDGQGRP HHHHHHHEECCCCCC	8.85	-
510	Phosphorylation	DGQGRPRTSQSEETR CCCCCCCCCCCCCEE	33.53	25403869
511	Phosphorylation	GQGRPRTSQSEETRV CCCCCCCCCCCCEEE	32.75	25403869
513	Phosphorylation	GRPRTSQSEETRVWH CCCCCCCCCCEEEEE	36.85	25403869
516	Phosphorylation	RTSQSEETRVWHRPD CCCCCCCEEEEECCC	26.86	25403869
534	Phosphorylation	QNVHFHCSGAPVAPL EEEEEEECCCCCCCC	29.44	27097102

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
254	T	Phosphorylation	Kinase	CAMK2A	P11275	PSP
254	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
280	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
287	T	Phosphorylation	Kinase	CAM-KII_GROUP	-	PhosphoELM
287	T	Phosphorylation	Kinase	CAMK2A	P11275	PSP
287	T	Phosphorylation	Kinase	CAMK2-FAMILY	-	GPS
287	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
306	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
307	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
311	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
315	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
315	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
320	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
321	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
325	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
327	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
328	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
331	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
333	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
334	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
343	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
358	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
363	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
367	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
371	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
381	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
382	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
397	S	Phosphorylation	Kinase	CAMK2B	P08413	PSP
400	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP
401	T	Phosphorylation	Kinase	CAMK2B	P08413	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
287	T	Phosphorylation	12873385
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin.
287	T	Phosphorylation	12873385
Phosphorylation of Thr-287 locks the kinase into an activated state.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KCC2B_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDC20_RAT	Cdc20	physical	21725312
1433E_RAT	Ywhae	physical	21725312
TBG1_RAT	Tubg1	physical	21725312

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KCC2B_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Regulation and function of Ca2+-calmodulin-dependent protein kinaseII of fast-twitch rat skeletal muscle."; Rose A.J., Alsted T.J., Kobberoe J.B., Richter E.A.; J. Physiol. (Lond.) 580:993-1005(2007). Cited for: FUNCTION, INDUCTION, AND PHOSPHORYLATION AT THR-287.	17272343 [Abstract]

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