BRAT1_HUMAN - dbPTM
BRAT1_HUMAN - PTM Information in dbPTM
Basic Information of Protein
UniProt ID BRAT1_HUMAN
UniProt AC Q6PJG6
Protein Name BRCA1-associated ATM activator 1
Gene Name BRAT1
Organism Homo sapiens (Human).
Sequence Length 821
Subcellular Localization Nucleus . Cytoplasm . Present at double strand breaks (DSBs)following ionizing radiation treatment. The ubiquitinated form localizes in the nucleus in a NDFIP1-dependent manner.
Protein Description Involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress. [PubMed: 16452482]
Protein Sequence MDPECAQLLPALCAVLVDPRQPVADDTCLEKLLDWFKTVTEGESSVVLLQEHPCLVELLSHVLKVQDLSSGVLSFSLRLAGTFAAQENCFQYLQQGELLPGLFGEPGPLGRATWAVPTVRSGWIQGLRSLAQHPSALRFLADHGAVDTIFSLQGDSSLFVASAASQLLVHVLALSMRGGAEGQPCLPGGDWPACAQKIMDHVEESLCSAATPKVTQALNVLTTTFGRCQSPWTEALWVRLSPRVACLLERDPIPAAHSFVDLLLCVARSPVFSSSDGSLWETVARALSCLGPTHMGPLALGILKLEHCPQALRTQAFQVLLQPLACVLKATVQAPGPPGLLDGTADDATTVDTLLASKSSCAGLLCRTLAHLEELQPLPQRPSPWPQASLLGATVTVLRLCDGSAAPASSVGGHLCGTLAGCVRVQRAALDFLGTLSQGTGPQELVTQALAVLLECLESPGSSPTVLKKAFQATLRWLLSSPKTPGCSDLGPLIPQFLRELFPVLQKRLCHPCWEVRDSALEFLTQLSRHWGGQADFRCALLASEVPQLALQLLQDPESYVRASAVTAMGQLSSQGLHAPTSPEHAEARQSLFLELLHILSVDSEGFPRRAVMQVFTEWLRDGHADAAQDTEQFVATVLQAASRDLDWEVRAQGLELALVFLGQTLGPPRTHCPYAVALPEVAPAQPLTEALRALCHVGLFDFAFCALFDCDRPVAQKSCDLLLFLRDKIASYSSLREARGSPNTASAEATLPRWRAGEQAQPPGDQEPEAVLAMLRSLDLEGLRSTLAESSDHVEKSPQSLLQDMLATGGFLQGDEADCY
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure		 ASA (%) Reference Orthologous
Protein Cluster
31UbiquitinationADDTCLEKLLDWFKT
CCCHHHHHHHHHHHH		41.2121987572
38UbiquitinationKLLDWFKTVTEGESS
HHHHHHHHCCCCCCC		24.1621963094
74PhosphorylationDLSSGVLSFSLRLAG
CCCCCHHHEEHHHHH		15.4724719451
76PhosphorylationSSGVLSFSLRLAGTF
CCCHHHEEHHHHHHH		14.8124719451
121PhosphorylationWAVPTVRSGWIQGLR
CCCCCCHHHHHHHHH		33.8621406692
129UbiquitinationGWIQGLRSLAQHPSA
HHHHHHHHHHHCHHH		32.8521963094
183UbiquitinationMRGGAEGQPCLPGGD
HCCCCCCCCCCCCCC		19.6221963094
197UbiquitinationDWPACAQKIMDHVEE
CCHHHHHHHHHHHHH		22.82-
199 (in isoform 3)Ubiquitination-3.6221890473
199UbiquitinationPACAQKIMDHVEESL
HHHHHHHHHHHHHHH		3.6221890473
212UbiquitinationSLCSAATPKVTQALN
HHHHCCCHHHHHHHH		25.1321963094
213UbiquitinationLCSAATPKVTQALNV
HHHCCCHHHHHHHHH		54.2621963094
230PhosphorylationTTFGRCQSPWTEALW
HCCCCCCCCCCHHHH		26.4824719451
258PhosphorylationDPIPAAHSFVDLLLC
CCCCCHHHHHHHHHH		23.4124114839
294UbiquitinationLSCLGPTHMGPLALG
HHHHCCCCCCCHHHH		23.4927667366
303UbiquitinationGPLALGILKLEHCPQ
CCHHHHHHHHHCCCH		5.0021963094
304UbiquitinationPLALGILKLEHCPQA
CHHHHHHHHHCCCHH		50.3221963094
308UbiquitinationGILKLEHCPQALRTQ
HHHHHHCCCHHHHHH		1.6321963094
332UbiquitinationACVLKATVQAPGPPG
HHHHHHHHCCCCCCC		5.6623000965
336UbiquitinationKATVQAPGPPGLLDG
HHHHCCCCCCCCCCC		44.0121963094
357UbiquitinationTVDTLLASKSSCAGL
HHHHHHHCHHHHHHH		32.8021963094
358UbiquitinationVDTLLASKSSCAGLL
HHHHHHCHHHHHHHH		40.5621963094
360UbiquitinationTLLASKSSCAGLLCR
HHHHCHHHHHHHHHH		16.2423000965
468UbiquitinationGSSPTVLKKAFQATL
CCCHHHHHHHHHHHH		37.4127667366
469UbiquitinationSSPTVLKKAFQATLR
CCHHHHHHHHHHHHH		50.7227667366
482UbiquitinationLRWLLSSPKTPGCSD
HHHHHCCCCCCCCCC		40.9621963094
483 (in isoform 1)Ubiquitination-70.1121890473
483UbiquitinationRWLLSSPKTPGCSDL
HHHHCCCCCCCCCCC		70.1121906983
484PhosphorylationWLLSSPKTPGCSDLG
HHHCCCCCCCCCCCH		28.4427050516
506UbiquitinationRELFPVLQKRLCHPC
HHHHHHHHHHHCCCC		29.1123000965
507UbiquitinationELFPVLQKRLCHPCW
HHHHHHHHHHCCCCH		43.0821890473
507AcetylationELFPVLQKRLCHPCW
HHHHHHHHHHCCCCH		43.0825953088
507UbiquitinationELFPVLQKRLCHPCW
HHHHHHHHHHCCCCH		43.0823000965
507 (in isoform 1)Ubiquitination-43.0821890473
510UbiquitinationPVLQKRLCHPCWEVR
HHHHHHHCCCCHHHH		3.8021963094
511UbiquitinationVLQKRLCHPCWEVRD
HHHHHHCCCCHHHHH		25.7421963094
519PhosphorylationPCWEVRDSALEFLTQ
CCHHHHHHHHHHHHH		24.9621712546
528PhosphorylationLEFLTQLSRHWGGQA
HHHHHHHHHHCCCCC		16.5721712546
534UbiquitinationLSRHWGGQADFRCAL
HHHHCCCCCHHHHHH		33.5423000965
535UbiquitinationSRHWGGQADFRCALL
HHHCCCCCHHHHHHH		22.6023000965
554UbiquitinationPQLALQLLQDPESYV
HHHHHHHHCCHHHHH		3.3327667366
564PhosphorylationPESYVRASAVTAMGQ
HHHHHHHHHHHHHHH		16.8328464451
567PhosphorylationYVRASAVTAMGQLSS
HHHHHHHHHHHHHHH		15.6223927012
573PhosphorylationVTAMGQLSSQGLHAP
HHHHHHHHHCCCCCC		16.8430266825
574PhosphorylationTAMGQLSSQGLHAPT
HHHHHHHHCCCCCCC		36.5430266825
581PhosphorylationSQGLHAPTSPEHAEA
HCCCCCCCCHHHHHH		58.6630266825
582PhosphorylationQGLHAPTSPEHAEAR
CCCCCCCCHHHHHHH		27.5130266825
622UbiquitinationVFTEWLRDGHADAAQ
HHHHHHHHCCCCHHH		51.5716196087
642UbiquitinationVATVLQAASRDLDWE
HHHHHHHHHCCCCHH		7.6727667366
671PhosphorylationQTLGPPRTHCPYAVA
CCCCCCCCCCCCEEE		33.3421945579
675PhosphorylationPPRTHCPYAVALPEV
CCCCCCCCEEECCCC		21.0721945579
689PhosphorylationVAPAQPLTEALRALC
CCCCCHHHHHHHHHH		26.3721945579
710UbiquitinationFAFCALFDCDRPVAQ
HHHHHHHCCCCHHHH		33.9716196087
728UbiquitinationDLLLFLRDKIASYSS
CHHHHHHHHHHHHHH		49.1927667366
729UbiquitinationLLLFLRDKIASYSSL
HHHHHHHHHHHHHHH		33.4721890473
729UbiquitinationLLLFLRDKIASYSSL
HHHHHHHHHHHHHHH		33.4727667366
729 (in isoform 1)Ubiquitination-33.4721890473
732PhosphorylationFLRDKIASYSSLREA
HHHHHHHHHHHHHHH		29.08-
735PhosphorylationDKIASYSSLREARGS
HHHHHHHHHHHHHCC		24.1828464451
742PhosphorylationSLREARGSPNTASAE
HHHHHHCCCCCCCHH		14.9319664994
745PhosphorylationEARGSPNTASAEATL
HHHCCCCCCCHHCCC		25.9323927012
747PhosphorylationRGSPNTASAEATLPR
HCCCCCCCHHCCCCC		25.3023927012
751PhosphorylationNTASAEATLPRWRAG
CCCCHHCCCCCCCCC		28.8823927012
757UbiquitinationATLPRWRAGEQAQPP
CCCCCCCCCCCCCCC		20.9327667366
788UbiquitinationLEGLRSTLAESSDHV
HHHHHHHHHHCCCCH		5.4027667366
789UbiquitinationEGLRSTLAESSDHVE
HHHHHHHHHCCCCHH		18.0827667366
792PhosphorylationRSTLAESSDHVEKSP
HHHHHHCCCCHHHCH		24.2025159151
796UbiquitinationAESSDHVEKSPQSLL
HHCCCCHHHCHHHHH		44.3816196087
797UbiquitinationESSDHVEKSPQSLLQ
HCCCCHHHCHHHHHH		67.3616196087
798PhosphorylationSSDHVEKSPQSLLQD
CCCCHHHCHHHHHHH		18.0525159151
801PhosphorylationHVEKSPQSLLQDMLA
CHHHCHHHHHHHHHH		34.1425627689
816UbiquitinationTGGFLQGDEADCY--
HCCCCCCCCCCCC--		34.6427667366
817UbiquitinationGGFLQGDEADCY---
CCCCCCCCCCCC---		53.9127667366
825UbiquitinationADCY-----------
CCCC-----------		16196087
856Ubiquitination------------------------------------------
------------------------------------------		16196087
857Ubiquitination-------------------------------------------
-------------------------------------------		16196087
884Ubiquitination----------------------------------------------------------------------
----------------------------------------------------------------------		16196087
885Ubiquitination-----------------------------------------------------------------------
-----------------------------------------------------------------------		16196087
Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources
-KUbiquitinationE3 ubiquitin ligaseNEDD4P46934
PMID:22199232
-KUbiquitinationE3 ubiquitin ligaseNEDD4LQ96PU5
PMID:22199232
-KUbiquitinationE3 ubiquitin ligaseITCHQ96J02
PMID:22199232
Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of BRAT1_HUMAN !!
Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10) 		Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of BRAT1_HUMAN !!
Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
UBP48_HUMANUSP48physical
22863883
RPTOR_HUMANRPTORphysical
25657994
MTOR_HUMANMTORphysical
25657994
FANCA_HUMANFANCAphysical
28215707
Drug and Disease Associations
Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
614498Rigidity and multifocal seizure syndrome, lethal neonatal (RMFSL)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of BRAT1_HUMAN

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, AND MASSSPECTROMETRY.
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, AND MASSSPECTROMETRY.
"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, AND MASSSPECTROMETRY.
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, AND MASSSPECTROMETRY.
"Evaluation of the low-specificity protease elastase for large-scalephosphoproteome analysis.";
Wang B., Malik R., Nigg E.A., Korner R.;
Anal. Chem. 80:9526-9533(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, AND MASSSPECTROMETRY.
"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization.";
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.;
Nat. Biotechnol. 24:1285-1292(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, AND MASSSPECTROMETRY.

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