ATG3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ATG3_MOUSE
• UniProt AC: Q9CPX6
• Protein Name: Ubiquitin-like-conjugating enzyme ATG3
• Gene Name: Atg3
• Organism: Mus musculus (Mouse).
• Sequence Length: 314
• Subcellular Localization: Cytoplasm.
• Protein Description: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway..
• Protein Sequence: MQNVINTVKGKALEVAEYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTDKQFLVTKNVPCYKRCKQMEYSDELEAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLESKDSIKLQDCSALCDEEDEEDEGEAADMEEYEESGLLETDEATLDTRKIVEACKAKADAGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYTRHFTM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MQNVINTV -------CCCHHHHH	6.20	-
11	Ubiquitination	VINTVKGKALEVAEY HHHHHHHHHHHHHHH	44.04	22790023
18	Phosphorylation	KALEVAEYLTPVLKE HHHHHHHHHHHHHCH	13.21	25159016
20	Phosphorylation	LEVAEYLTPVLKESK HHHHHHHHHHHCHHH	15.50	26643407
24	Ubiquitination	EYLTPVLKESKFKET HHHHHHHCHHHCCCC	61.24	22790023
77	Acetylation	DKQFLVTKNVPCYKR CCCEEEECCCCHHHH	48.76	22826441
81	Glutathionylation	LVTKNVPCYKRCKQM EEECCCCHHHHHCCC	5.64	24333276
98	Ubiquitination	SDELEAIIEEDDGDG CHHHHHHEEECCCCC	6.46	27667366
130	Phosphorylation	VKEITLESKDSIKLQ HHHCCCCCCCCEEEE	44.89	30352176
131	Ubiquitination	KEITLESKDSIKLQD HHCCCCCCCCEEEEE	46.23	22790023
133	Phosphorylation	ITLESKDSIKLQDCS CCCCCCCCEEEEEHH	25.92	27600695
140	Phosphorylation	SIKLQDCSALCDEED CEEEEEHHHHCCCCC	32.34	29899451
160	Phosphorylation	EAADMEEYEESGLLE CCCCHHHHHHHCCCC	16.22	24224561
182	Glutathionylation	TRKIVEACKAKADAG HHHHHHHHHHHCCCC	2.52	24333276
185	Ubiquitination	IVEACKAKADAGGED HHHHHHHHCCCCCCC	32.09	27667366
203	Phosphorylation	QTRTYDLYITYDKYY EECEEEEEEEECCCC	6.58	-
208	Ubiquitination	DLYITYDKYYQTPRL EEEEEECCCCCCCEE	35.13	22790023
208	Acetylation	DLYITYDKYYQTPRL EEEEEECCCCCCCEE	35.13	23954790
244	Phosphorylation	SQDHVKKTVTIENHP CHHHCCCEEEECCCC	19.71	-
246	Phosphorylation	DHVKKTVTIENHPHL HHCCCEEEECCCCCC	28.01	-
259	S-palmitoylation	HLPPPPMCSVHPCRH CCCCCCCCEECCCCC	4.79	28680068
264	S-palmitoylation	PMCSVHPCRHAEVMK CCCEECCCCCHHHHH	2.76	28680068
306	Phosphorylation	AVIPTIEYDYTRHFT HHCCCCCCCCCCCCC	15.48	26643407
308	Phosphorylation	IPTIEYDYTRHFTM- CCCCCCCCCCCCCC-	12.55	26643407
309	Phosphorylation	PTIEYDYTRHFTM-- CCCCCCCCCCCCC--	18.12	26643407
313	Phosphorylation	YDYTRHFTM------ CCCCCCCCC------	18.82	26643407

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
203	Y	Phosphorylation	Kinase	PTK2B	Q14289	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ATG3_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ATG3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ATG12_HUMAN	ATG12	physical	20723759
ATG7_MOUSE	Atg7	physical	22170151
ATG12_MOUSE	Atg12	physical	24191030
ATG7_MOUSE	Atg7	physical	24191030

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.