AT1A2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: AT1A2_RAT
• UniProt AC: P06686
• Protein Name: Sodium/potassium-transporting ATPase subunit alpha-2
• Gene Name: Atp1a2
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 1020
• Subcellular Localization: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
• Protein Description: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients..
• Protein Sequence: MGRGAGREYSPAATTAENGGGKKKQKEKELDELKKEVAMDDHKLSLDELGRKYQVDLSKGLTNQRAQDILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGALLCFLAYGILAAMEDEPSNDNLYLGIVLAAVVIVTGCFSYYQEAKSSKIMDSFKNMVPQQALVIREGEKMQINAEEVVVGDLVEVKGGDRVPADLRIISSHGCKVDNSSLTGESEPQTRSPEFTHENPLETRNICFFSTNCVEGTARGIVIATGDRTVMGRIATLASGLEVGQTPIAMEIEHFIQLITGVAVFLGVSFFVLSLILGYSWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALSRIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSIHEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSGKFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPREAKACVVHGSDLKDMTSEQLDEILRDHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGIAMGISGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIIANIPLPLGTVTILCIDLGTDMVPAISLAYEAAESDIMKRQPRNSQTDKLVNERLISMAYGQIGMIQALGGFFTYFVILAENGFLPSRLLGIRLDWDDRTTNDLEDSYGQEWTYEQRKVVEFTCHTAFFASIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLLEETALAAFLSYCPGMGVALRMYPLKVTWWFCAFPYSLLIFIYDEVRKLILRRYPGGWVEKETYY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	GRGAGREYSPAATTA CCCCCCCCCCCCCCC	20.70	27097102
10	Phosphorylation	RGAGREYSPAATTAE CCCCCCCCCCCCCCC	11.87	27097102
34	Acetylation	EKELDELKKEVAMDD HHHHHHHHHHHCCCC	44.85	22902405
35	Ubiquitination	KELDELKKEVAMDDH HHHHHHHHHHCCCCC	70.78	-
43	Acetylation	EVAMDDHKLSLDELG HHCCCCCCCCHHHHC	46.92	22902405
43	Ubiquitination	EVAMDDHKLSLDELG HHCCCCCCCCHHHHC	46.92	-
45	Phosphorylation	AMDDHKLSLDELGRK CCCCCCCCHHHHCHH	38.28	-
53	Phosphorylation	LDELGRKYQVDLSKG HHHHCHHHCCCCCCC	16.65	-
58	Phosphorylation	RKYQVDLSKGLTNQR HHHCCCCCCCCCCHH	21.88	25403869
59	Acetylation	KYQVDLSKGLTNQRA HHCCCCCCCCCCHHH	66.30	22902405
59	Ubiquitination	KYQVDLSKGLTNQRA HHCCCCCCCCCCHHH	66.30	-
154	Ubiquitination	YQEAKSSKIMDSFKN HHHHHHCHHHHHHHH	49.49	-
160	Ubiquitination	SKIMDSFKNMVPQQA CHHHHHHHHCCCCEE	48.35	-
192	Ubiquitination	VGDLVEVKGGDRVPA EECEEEEECCCCCCC	43.61	-
210	Ubiquitination	IISSHGCKVDNSSLT EEECCCCEECCCCCC	58.16	-
214	Phosphorylation	HGCKVDNSSLTGESE CCCEECCCCCCCCCC	23.43	29779826
215	Phosphorylation	GCKVDNSSLTGESEP CCEECCCCCCCCCCC	35.88	30411139
217	Phosphorylation	KVDNSSLTGESEPQT EECCCCCCCCCCCCC	40.63	27097102
220	Phosphorylation	NSSLTGESEPQTRSP CCCCCCCCCCCCCCC	56.79	27097102
224	Phosphorylation	TGESEPQTRSPEFTH CCCCCCCCCCCCCCC	43.78	28689409
226	Phosphorylation	ESEPQTRSPEFTHEN CCCCCCCCCCCCCCC	31.97	25403869
230	Phosphorylation	QTRSPEFTHENPLET CCCCCCCCCCCCCCC	26.98	25403869
273	Phosphorylation	GRIATLASGLEVGQT HHHHHHHHCCCCCCC	47.20	-
364	Phosphorylation	KNLEAVETLGSTSTI CCHHHHHHHCCCCCC	29.80	28551015
367	Phosphorylation	EAVETLGSTSTICSD HHHHHHCCCCCCCCC	23.46	28551015
368	Phosphorylation	AVETLGSTSTICSDK HHHHHCCCCCCCCCC	28.26	28551015
369	Phosphorylation	VETLGSTSTICSDKT HHHHCCCCCCCCCCC	19.92	28551015
370	Phosphorylation	ETLGSTSTICSDKTG HHHCCCCCCCCCCCC	26.54	28551015
373	Phosphorylation	GSTSTICSDKTGTLT CCCCCCCCCCCCCCC	37.99	28551015
376	Phosphorylation	STICSDKTGTLTQNR CCCCCCCCCCCCCCC	40.15	22108457
378	Phosphorylation	ICSDKTGTLTQNRMT CCCCCCCCCCCCCEE	31.68	28551015
380	Phosphorylation	SDKTGTLTQNRMTVA CCCCCCCCCCCEEEE	24.52	28551015
385	Phosphorylation	TLTQNRMTVAHMWFD CCCCCCEEEEHHHCC	16.00	25403869
400	Phosphorylation	NQIHEADTTEDQSGA CCCCCCCCCCCCCCC	38.85	25403869
413	Phosphorylation	GATFDKRSPTWTALS CCCCCCCCCHHHHHH	32.21	22673903
415	Phosphorylation	TFDKRSPTWTALSRI CCCCCCCHHHHHHHH	36.37	22673903
417	Phosphorylation	DKRSPTWTALSRIAG CCCCCHHHHHHHHHH	21.87	22673903
420	Phosphorylation	SPTWTALSRIAGLCN CCHHHHHHHHHHHHH	21.12	28689409
432	Acetylation	LCNRAVFKAGQENIS HHHHHHHHCCHHCCC	45.04	22902405
432	Ubiquitination	LCNRAVFKAGQENIS HHHHHHHHCCHHCCC	45.04	-
439	Phosphorylation	KAGQENISVSKRDTA HCCHHCCCCCCCCCC	31.74	30411139
441	Phosphorylation	GQENISVSKRDTAGD CHHCCCCCCCCCCCC	18.38	25403869
442	Ubiquitination	QENISVSKRDTAGDA HHCCCCCCCCCCCCC	52.73	-
445	Phosphorylation	ISVSKRDTAGDASES CCCCCCCCCCCCCHH	36.25	28551015
450	Phosphorylation	RDTAGDASESALLKC CCCCCCCCHHHHHHH	36.50	28551015
452	Phosphorylation	TAGDASESALLKCIE CCCCCCHHHHHHHHH	23.01	28551015
461	Phosphorylation	LLKCIELSCGSVRKM HHHHHHHHCCCHHHH	12.02	28551015
464	Phosphorylation	CIELSCGSVRKMRDR HHHHHCCCHHHHHCC	24.07	28551015
482	Phosphorylation	VAEIPFNSTNKYQLS EEECCCCCCCCEEEE	33.46	25403869
483	Phosphorylation	AEIPFNSTNKYQLSI EECCCCCCCCEEEEE	36.51	28551015
485	Acetylation	IPFNSTNKYQLSIHE CCCCCCCCEEEEEEE	34.17	22902405
496	Phosphorylation	SIHEREDSPQSHVLV EEEECCCCCCCEEEE	21.78	25403869
499	Phosphorylation	EREDSPQSHVLVMKG ECCCCCCCEEEEECC	20.96	28551015
505	Acetylation	QSHVLVMKGAPERIL CCEEEEECCCHHHHH	44.43	22902405
516	Phosphorylation	ERILDRCSTILVQGK HHHHHHCCEEEECCC	21.26	25403869
529	Acetylation	GKEIPLDKEMQDAFQ CCCCCCCHHHHHHHH	63.87	22902405
529	Ubiquitination	GKEIPLDKEMQDAFQ CCCCCCCHHHHHHHH	63.87	-
539	Phosphorylation	QDAFQNAYMELGGLG HHHHHHHHHHHCCHH	9.93	-
559	Phosphorylation	FCQLNLPSGKFPRGF EEEECCCCCCCCCCC	58.75	28551015
561	Ubiquitination	QLNLPSGKFPRGFKF EECCCCCCCCCCCCC	57.44	-
561	Acetylation	QLNLPSGKFPRGFKF EECCCCCCCCCCCCC	57.44	22902405
567	Ubiquitination	GKFPRGFKFDTDELN CCCCCCCCCCHHHCC	45.19	-
567	Acetylation	GKFPRGFKFDTDELN CCCCCCCCCCHHHCC	45.19	22902405
570	Phosphorylation	PRGFKFDTDELNFPT CCCCCCCHHHCCCCH	34.21	-
587	Phosphorylation	LCFVGLMSMIDPPRA HHHHHHHHCCCCCHH	20.03	-
602	Ubiquitination	AVPDAVGKCRSAGIK CCCCHHHHHHHCCCE	21.63	-
609	Acetylation	KCRSAGIKVIMVTGD HHHHCCCEEEEEECC	25.45	-
622	Acetylation	GDHPITAKAIAKGVG CCCCCCHHHHHHCCE	31.51	42502065
632	Phosphorylation	AKGVGIISEGNETVE HHCCEEEECCCCCHH	37.21	25403869
637	Phosphorylation	IISEGNETVEDIAAR EEECCCCCHHHHHHH	32.94	28551015
650	Phosphorylation	ARLNIPVSQVNPREA HHCCCCHHHCCHHHC	24.05	27097102
658	Ubiquitination	QVNPREAKACVVHGS HCCHHHCCEEEEECH	37.25	-
671	Phosphorylation	GSDLKDMTSEQLDEI CHHHHCCCHHHHHHH	38.97	22673903
672	Phosphorylation	SDLKDMTSEQLDEIL HHHHCCCHHHHHHHH	19.39	22673903
719	Phosphorylation	TGDGVNDSPALKKAD ECCCCCCCHHHHHCC	13.58	30240740
723	Ubiquitination	VNDSPALKKADIGIA CCCCHHHHHCCEEEE	48.28	-
723	Acetylation	VNDSPALKKADIGIA CCCCHHHHHCCEEEE	48.28	4256543
724	Ubiquitination	NDSPALKKADIGIAM CCCHHHHHCCEEEEC	52.74	-
724	Acetylation	NDSPALKKADIGIAM CCCHHHHHCCEEEEC	52.74	4256545
826	Phosphorylation	LAYEAAESDIMKRQP HHHHHHHHHHHHCCC	27.92	-
840	Acetylation	PRNSQTDKLVNERLI CCCCHHHHHHHHHHH	58.69	22902405
840	Ubiquitination	PRNSQTDKLVNERLI CCCCHHHHHHHHHHH	58.69	-
891	Phosphorylation	RLDWDDRTTNDLEDS ECCCCCCCCCCCHHH	36.62	12692561
940	Phosphorylation	ICKTRRNSVFQQGMK EECCCCCHHHHHHHC	23.61	25403869
1016	Ubiquitination	YPGGWVEKETYY--- CCCCCEEEEECC---	45.82	-
1019	Phosphorylation	GWVEKETYY------ CCEEEEECC------	14.31	-
1020	Phosphorylation	WVEKETYY------- CEEEEECC-------	21.35	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
940	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of AT1A2_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of AT1A2_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ITPR1_RAT	Itpr1	physical	14593108
NAC1_RAT	Slc8a1	physical	14593108
AT2B1_RAT	Atp2b1	physical	14593108
SPTN1_RAT	Sptan1	physical	14593108

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.