ASPG_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ASPG_HUMAN
• UniProt AC: P20933
• Protein Name: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
• Gene Name: AGA
• Organism: Homo sapiens (Human).
• Sequence Length: 346
• Subcellular Localization: Lysosome.
• Protein Description: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins..
• Protein Sequence: MARKSNLPVLLVPFLLCQALVRCSSPLPLVVNTWPFKNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDELGETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTTASQALHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSEKNQPTEEKVDCI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
24	Blocked amino end (Ser)	CQALVRCSSPLPLVV HHHHHHCCCCCCEEE	25.15	-
24	Blocked amino end	CQALVRCSSPLPLVV HHHHHHCCCCCCEEE	25.15	-
38	N-linked_Glycosylation	VNTWPFKNATEAAWR EECCCCCCHHHHHHH	53.10	8846222
38	N-linked_Glycosylation	VNTWPFKNATEAAWR EECCCCCCHHHHHHH	53.10	9362483
63	Sulfoxidation	AVESGCAMCEREQCD HHHHCCCCCCHHCCC	2.58	30846556
72	Phosphorylation	EREQCDGSVGFGGSP CHHCCCCCCCCCCCH	13.05	22210691
78	Phosphorylation	GSVGFGGSPDELGET CCCCCCCCHHHCCCC	29.56	22210691
109	Ubiquitination	VGDLRRIKNAIGVAR HHHHHHHHHHHHHHH	38.22	29967540
178	Phosphorylation	VIPDPSKYCGPYKPP CCCCHHHCCCCCCCC	13.64	22817900
215	Phosphorylation	GMVVIHKTGHIAAGT EEEEEECCCEEECEE	21.60	50563901
223	Phosphorylation	GHIAAGTSTNGIKFK CEEECEECCCCEEEE	20.96	24719451
238	Phosphorylation	IHGRVGDSPIPGAGA EEEEECCCCCCCCCC	20.85	28111955
246	Phosphorylation	PIPGAGAYADDTAGA CCCCCCCCCCCCCCC	15.05	28111955
308	N-linked_Glycosylation	FGAVICANVTGSYGA CCEEEEEECCCCCHH	27.72	9362483
308	N-linked_Glycosylation	FGAVICANVTGSYGA CCEEEEEECCCCCHH	27.72	9362483
321	Phosphorylation	GAACNKLSTFTQFSF HHHHHHCCCCCEEEE	24.08	23663014
322	Phosphorylation	AACNKLSTFTQFSFM HHHHHCCCCCEEEEE	40.93	23663014
324	Phosphorylation	CNKLSTFTQFSFMVY HHHCCCCCEEEEEEE	28.88	23663014
327	Phosphorylation	LSTFTQFSFMVYNSE CCCCCEEEEEEECCC	11.56	23663014
331	Phosphorylation	TQFSFMVYNSEKNQP CEEEEEEECCCCCCC	10.94	23663014
333	Phosphorylation	FSFMVYNSEKNQPTE EEEEEECCCCCCCCH	32.06	23663014

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ASPG_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ASPG_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ASPG_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
ASPG_HUMAN	AGA	physical	9737998
PHOCN_HUMAN	MOB4	physical	26186194
HBB_HUMAN	HBB	physical	26186194
ZER1_HUMAN	ZER1	physical	26186194
PHOCN_HUMAN	MOB4	physical	28514442
HBB_HUMAN	HBB	physical	28514442
ZER1_HUMAN	ZER1	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 208400: Aspartylglucosaminuria (AGU)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"Purification and structure of human liver aspartylglucosaminidase.";
Rip J.W., Coulter-Mackie M.B., Rupar C.A., Gordon B.A.;
Biochem. J. 288:1005-1010(1992).
Cited for: PROTEIN SEQUENCE OF 25-44 AND 206-231, AND GLYCOSYLATION AT ASN-38.
PubMed: 1281977

"Cloning and sequence analysis of a cDNA for humanglycosylasparaginase. A single gene encodes the subunits of thislysosomal amidase.";
Fisher K.J., Tollersrud O.-K., Aronson N.N. Jr.;
FEBS Lett. 269:440-444(1990).
Cited for: NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT SER-149.
PubMed: 2401370