ALG3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ALG3_HUMAN
• UniProt AC: Q92685
• Protein Name: Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
• Gene Name: ALG3
• Organism: Homo sapiens (Human).
• Sequence Length: 438
• Subcellular Localization: Endoplasmic reticulum membrane ; Multi-pass membrane protein .
• Protein Description: Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol..
• Protein Sequence: MAAGLRKRGRSGSAAQAEGLCKQWLQRAWQERRLLLREPRYTLLVAACLCLAEVGITFWVIHRVAYTEIDWKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVFLIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMNVLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLPEALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGICFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSAALHICHAVILLQLWLGPQPFPKSTQHSKKAH

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	GLRKRGRSGSAAQAE CCCCCCCCCCHHHHH	39.67	29255136
13	Phosphorylation	RKRGRSGSAAQAEGL CCCCCCCCHHHHHHH	22.98	29255136
22	Ubiquitination	AQAEGLCKQWLQRAW HHHHHHHHHHHHHHH	49.94	21890473
166	Phosphorylation	CASYRVHSIFVLRLF HHHHHHHCHHHHHHC	18.18	46162275
312	Phosphorylation	ALCRWHRTGESILSL HHHHHHHCCHHHHHH	32.20	21406692
315	Phosphorylation	RWHRTGESILSLLRD HHHHCCHHHHHHHCC	30.12	21406692
318	Phosphorylation	RTGESILSLLRDPSK HCCHHHHHHHCCCCC	24.56	21406692
325	Ubiquitination	SLLRDPSKRKVPPQP HHHCCCCCCCCCCCC	62.72	21890473
355	Phosphorylation	IGICFSRSLHYQFYV HHHHHHHCHHHHHHH	20.49	28450419
358	Phosphorylation	CFSRSLHYQFYVWYF HHHHCHHHHHHHHHH	12.95	28450419
361	Phosphorylation	RSLHYQFYVWYFHTL HCHHHHHHHHHHHHH	3.73	28450419
364	Phosphorylation	HYQFYVWYFHTLPYL HHHHHHHHHHHHHHH	3.87	28450419
367	Phosphorylation	FYVWYFHTLPYLLWA HHHHHHHHHHHHHHH	21.38	28450419
370	Phosphorylation	WYFHTLPYLLWAMPA HHHHHHHHHHHHCCH	19.96	28450419

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ALG3_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ALG3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ALG3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GFAP_HUMAN	GFAP	physical	26186194
AL3B1_HUMAN	ALDH3B1	physical	26186194
T10IP_HUMAN	TSGA10IP	physical	26186194
KLH14_HUMAN	KLHL14	physical	26186194
CREB3_HUMAN	CREB3	physical	21516116
T10IP_HUMAN	TSGA10IP	physical	28514442
KLH14_HUMAN	KLHL14	physical	28514442
GFAP_HUMAN	GFAP	physical	28514442
AL3B1_HUMAN	ALDH3B1	physical	28514442

Drug and Disease Associations

• Kegg Disease
• H00118: Congenital disorders of glycosylation (CDG) type I
• OMIM Disease
• 601110: Congenital disorder of glycosylation 1D (CDG1D)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13, AND MASSSPECTROMETRY.
PubMed: 19413330

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13, AND MASSSPECTROMETRY.
PubMed: 18691976