dbPTM

ZO3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ZO3_HUMAN
UniProt AC	O95049
Protein Name	Tight junction protein ZO-3
Gene Name	TJP3
Organism	Homo sapiens (Human).
Sequence Length	919
Subcellular Localization	Cell membrane Peripheral membrane protein Cytoplasmic side . Cell junction, tight junction . Nucleus . Exhibits predominant nuclear expression in proliferating cells but is exclusively junctionally expressed after confluence is reached (PubMed:23
Protein Description	TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. [PubMed: 16129888 The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions]
Protein Sequence	MEELTIWEQHTATLSKDPRRGFGIAISGGRDRPGGSMVVSDVVPGGPAEGRLQTGDHIVMVNGVSMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPPPPRHAQRSPEASQTDSPVESPRLRRESSVDSRTISEPDEQRSELPRESSYDIYRVPSSQSMEDRGYSPDTRVVRFLKGKSIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDQILQVNDVPFQNLTREEAVQFLLGLPPGEEMELVTQRKQDIFWKMVQSRVGDSFYIRTHFELEPSPPSGLGFTRGDVFHVLDTLHPGPGQSHARGGHWLAVRMGRDLREQERGIIPNQSRAEQLASLEAAQRAVGVGPGSSAGSNARAEFWRLRGLRRGAKKTTQRSREDLSALTRQGRYPPYERVVLREASFKRPVVILGPVADIAMQKLTAEMPDQFEIAETVSRTDSPSKIIKLDTVRVIAEKDKHALLDVTPSAIERLNYVQYYPIVVFFIPESRPALKALRQWLAPASRRSTRRLYAQAQKLRKHSSHLFTATIPLNGTSDTWYQELKAIIREQQTRPIWTAEDQLDGSLEDNLDLPHHGLADSSADLSCDSRVNSDYETDGEGGAYTDGEGYTDGEGGPYTDVDDEPPAPALARSSEPVQADESQSPRDRGRISAHQGAQVDSRHPQGQWRQDSMRTYEREALKKKFMRVHDAESSDEDGYDWGPATDL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
27	Phosphorylation	RGFGIAISGGRDRPG CCCCEEEECCCCCCC	26.60	27251275
36	Phosphorylation	GRDRPGGSMVVSDVV CCCCCCCCEEEEECC	17.83	23312004
40	Phosphorylation	PGGSMVVSDVVPGGP CCCCEEEEECCCCCC	17.18	23312004
45	Phosphorylation	VVSDVVPGGPAEGRL EEEECCCCCCCCCCC	43.01	27251275
103	Phosphorylation	HLPATKASPSSPGRQ CCCCCCCCCCCCCCC	27.20	30278072
105	Phosphorylation	PATKASPSSPGRQDS CCCCCCCCCCCCCCC	46.82	26657352
106	Phosphorylation	ATKASPSSPGRQDSD CCCCCCCCCCCCCCC	34.46	30278072
112	Phosphorylation	SSPGRQDSDEDDGPQ CCCCCCCCCCCCCCC	34.26	30278072
121	Phosphorylation	EDDGPQRVEEVDQGR CCCCCCCCEEECCCC	6.57	27251275
130	Phosphorylation	EVDQGRGYDGDSSSG EECCCCCCCCCCCCC	18.52	22210691
134	Phosphorylation	GRGYDGDSSSGSGRS CCCCCCCCCCCCCCC	31.75	23909892
135	Phosphorylation	RGYDGDSSSGSGRSW CCCCCCCCCCCCCCC	43.38	23909892
136	Phosphorylation	GYDGDSSSGSGRSWD CCCCCCCCCCCCCCC	40.71	23909892
138	Phosphorylation	DGDSSSGSGRSWDER CCCCCCCCCCCCCHH	32.74	23909892
141	Phosphorylation	SSSGSGRSWDERSRR CCCCCCCCCCHHCCC	42.26	33259812
150	Phosphorylation	DERSRRPRPGRRGRA CHHCCCCCCCCCCCC	44.48	33259812
159	Phosphorylation	GRRGRAGSHGRRSPG CCCCCCCCCCCCCCC	23.06	22617229
164	Phosphorylation	AGSHGRRSPGGGSEA CCCCCCCCCCCCCHH	26.91	23927012
168	Phosphorylation	GRRSPGGGSEANGLA CCCCCCCCCHHHCEE	28.38	27251275
169	Phosphorylation	RRSPGGGSEANGLAL CCCCCCCCHHHCEEE	36.49	30266825
173	Phosphorylation	GGGSEANGLALVSGF CCCCHHHCEEECCCC	21.26	27251275
178	Phosphorylation	ANGLALVSGFKRLPR HHCEEECCCCCCCCC	39.09	23090842
203	Phosphorylation	VLVKRRDSEEFGVKL EEEECCCHHHHCCCC	36.90	28355574
212	Phosphorylation	EFGVKLGSQIFIKHI HHCCCCCCEEEEEEE	30.46	20068231
220	Phosphorylation	QIFIKHITDSGLAAR EEEEEEECCCCHHHH	24.67	20068231
221	Phosphorylation	IFIKHITDSGLAARH EEEEEECCCCHHHHH	39.85	27251275
222	Phosphorylation	FIKHITDSGLAARHR EEEEECCCCHHHHHC	27.36	23312004
259	Ubiquitination	DTRRLIEKSEGKLSL HHHHHHHHCCCCEEE	46.98	29967540
260	Phosphorylation	TRRLIEKSEGKLSLL HHHHHHHCCCCEEEE	38.50	23312004
265	Phosphorylation	EKSEGKLSLLVLRDR HHCCCCEEEEEEECC	24.12	28348404
268	Ubiquitination	EGKLSLLVLRDRGQF CCCEEEEEEECCCCE	5.10	29967540
274	Phosphorylation	LVLRDRGQFLVNIPP EEEECCCCEEEECCC	29.42	27251275
319	Phosphorylation	PPRHAQRSPEASQTD CCCCCCCCCCHHCCC	24.24	28355574
323	Phosphorylation	AQRSPEASQTDSPVE CCCCCCHHCCCCCCC	6.86	30278072
325	Phosphorylation	RSPEASQTDSPVESP CCCCHHCCCCCCCCH	51.42	23927012
327	Phosphorylation	PEASQTDSPVESPRL CCHHCCCCCCCCHHH	46.60	30278072
328	Phosphorylation	EASQTDSPVESPRLR CHHCCCCCCCCHHHH	43.47	27251275
331	Phosphorylation	QTDSPVESPRLRRES CCCCCCCCHHHHCCC	40.01	30278072
332	Phosphorylation	TDSPVESPRLRRESS CCCCCCCHHHHCCCC	55.77	27251275
336	Phosphorylation	VESPRLRRESSVDSR CCCHHHHCCCCCCCC	15.43	27251275
338	Phosphorylation	SPRLRRESSVDSRTI CHHHHCCCCCCCCCC	57.27	25849741
339	Phosphorylation	PRLRRESSVDSRTIS HHHHCCCCCCCCCCC	35.63	25849741
340	Phosphorylation	RLRRESSVDSRTISE HHHCCCCCCCCCCCC	43.22	27251275
342	Phosphorylation	RRESSVDSRTISEPD HCCCCCCCCCCCCCH	36.38	25849741
344	Phosphorylation	ESSVDSRTISEPDEQ CCCCCCCCCCCCHHH	59.41	23927012
346	Phosphorylation	SVDSRTISEPDEQRS CCCCCCCCCCHHHHC	26.31	23911959
347	Phosphorylation	VDSRTISEPDEQRSE CCCCCCCCCHHHHCC	50.54	27251275
352	Methylation	ISEPDEQRSELPRES CCCCHHHHCCCCCCC	33.57	115367603
353	Phosphorylation	SEPDEQRSELPRESS CCCHHHHCCCCCCCC	25.78	23927012
355	Phosphorylation	PDEQRSELPRESSYD CHHHHCCCCCCCCCC	34.06	27251275
357	Methylation	EQRSELPRESSYDIY HHHCCCCCCCCCCEE	31.45	115367607
359	Phosphorylation	RSELPRESSYDIYRV HCCCCCCCCCCEEEC	4.22	26657352
360	Phosphorylation	SELPRESSYDIYRVP CCCCCCCCCCEEECC	42.33	28796482
361	Phosphorylation	ELPRESSYDIYRVPS CCCCCCCCCEEECCC	53.58	28796482
364	Phosphorylation	RESSYDIYRVPSSQS CCCCCCEEECCCCCC	59.62	28796482
368	Phosphorylation	YDIYRVPSSQSMEDR CCEEECCCCCCCCCC	66.05	27273156
369	Phosphorylation	DIYRVPSSQSMEDRG CEEECCCCCCCCCCC	5.23	27273156
369	O-linked_Glycosylation	DIYRVPSSQSMEDRG CEEECCCCCCCCCCC	5.23	28580691
371	Phosphorylation	YRVPSSQSMEDRGYS EECCCCCCCCCCCCC	69.89	30278072
377	Phosphorylation	QSMEDRGYSPDTRVV CCCCCCCCCCCCHHH	1.47	27251275
378	Phosphorylation	SMEDRGYSPDTRVVR CCCCCCCCCCCHHHH	11.58	21815630
380	Phosphorylation	EDRGYSPDTRVVRFL CCCCCCCCCHHHHHH	2.79	27251275
381	Phosphorylation	DRGYSPDTRVVRFLK CCCCCCCCHHHHHHC	21.18	27794612
387	Phosphorylation	DTRVVRFLKGKSIGL CCHHHHHHCCCCEEE	50.21	27251275
479	Phosphorylation	SRVGDSFYIRTHFEL HHCCCCEEEEEEEEE	4.04	-
489	Phosphorylation	THFELEPSPPSGLGF EEEEECCCCCCCCCC	24.59	26657352
492	Phosphorylation	ELEPSPPSGLGFTRG EECCCCCCCCCCCCC	7.56	23312004
498	Phosphorylation	PSGLGFTRGDVFHVL CCCCCCCCCCEEEEE	13.01	27251275
518	Methylation	GPGQSHARGGHWLAV CCCCCCCCCCCEEEE	3.34	54560915
550	Phosphorylation	SRAEQLASLEAAQRA CHHHHHHHHHHHHHH	41.11	29743597
564	Phosphorylation	AVGVGPGSSAGSNAR HHCCCCCCCCCHHHH	35.02	23312004
565	Phosphorylation	VGVGPGSSAGSNARA HCCCCCCCCCHHHHH	4.28	25072903
568	Phosphorylation	GPGSSAGSNARAEFW CCCCCCCHHHHHHHH	7.04	25072903
573	Phosphorylation	AGSNARAEFWRLRGL CCHHHHHHHHHHHHH	20.06	27251275
587	Phosphorylation	LRRGAKKTTQRSRED HHHHCCHHCCCCHHH	39.94	27966365
588	Phosphorylation	RRGAKKTTQRSREDL HHHCCHHCCCCHHHH	4.49	26074081
591	Phosphorylation	AKKTTQRSREDLSAL CCHHCCCCHHHHHHH	3.57	30183078
596	Phosphorylation	QRSREDLSALTRQGR CCCHHHHHHHHHCCC	55.23	30278072
597	Phosphorylation	RSREDLSALTRQGRY CCHHHHHHHHHCCCC	35.00	27251275
599	Phosphorylation	REDLSALTRQGRYPP HHHHHHHHHCCCCCC	54.18	26074081
600	Phosphorylation	EDLSALTRQGRYPPY HHHHHHHHCCCCCCH	57.16	27251275
604	Phosphorylation	ALTRQGRYPPYERVV HHHHCCCCCCHHHHH	35.27	26074081
607	Phosphorylation	RQGRYPPYERVVLRE HCCCCCCHHHHHCCC	65.63	26074081
616	Phosphorylation	RVVLREASFKRPVVI HHHCCCCCCCCCEEE	28.00	24719451
625	Phosphorylation	KRPVVILGPVADIAM CCCEEEECHHHHHHH	5.37	27251275
648	Phosphorylation	DQFEIAETVSRTDSP CHHEEEEHHCCCCCC	34.42	28348404
650	Phosphorylation	FEIAETVSRTDSPSK HEEEEHHCCCCCCHH	27.83	28348404
652	Phosphorylation	IAETVSRTDSPSKII EEEHHCCCCCCHHEE	46.11	23312004
654	Phosphorylation	ETVSRTDSPSKIIKL EHHCCCCCCHHEEEE	24.61	25849741
656	Phosphorylation	VSRTDSPSKIIKLDT HCCCCCCHHEEEEEE	31.81	23312004
663	Phosphorylation	SKIIKLDTVRVIAEK HHEEEEEEEEEEEEC	2.47	28348404
679	Phosphorylation	KHALLDVTPSAIERL CCEEECCCHHHHHHC	19.06	21406692
681	Phosphorylation	ALLDVTPSAIERLNY EEECCCHHHHHHCCC	2.61	21406692
717	Phosphorylation	RQWLAPASRRSTRRL HHHHCHHCHHHHHHH	20.88	28348404
725	Phosphorylation	RRSTRRLYAQAQKLR HHHHHHHHHHHHHHH	40.14	28152594
778	Phosphorylation	AEDQLDGSLEDNLDL HHHHCCCCCCCCCCC	34.42	28348404
787	Phosphorylation	EDNLDLPHHGLADSS CCCCCCCCCCCCCCC	52.45	27251275
793	Phosphorylation	PHHGLADSSADLSCD CCCCCCCCCCCCCCC	13.50	28348404
794	Phosphorylation	HHGLADSSADLSCDS CCCCCCCCCCCCCCC	46.69	28348404
803	Phosphorylation	DLSCDSRVNSDYETD CCCCCCCCCCCCCCC	21.37	27251275
805	Phosphorylation	SCDSRVNSDYETDGE CCCCCCCCCCCCCCC	21.16	29802988
814	Phosphorylation	YETDGEGGAYTDGEG CCCCCCCCCCCCCCC	35.78	27251275
845	Phosphorylation	PAPALARSSEPVQAD CCCCCCCCCCCCCCC	32.45	29396449
846	Phosphorylation	APALARSSEPVQADE CCCCCCCCCCCCCCC	35.68	29396449
854	Phosphorylation	EPVQADESQSPRDRG CCCCCCCCCCHHHHC	33.31	22617229
855	Phosphorylation	PVQADESQSPRDRGR CCCCCCCCCHHHHCC	18.89	27251275
856	Phosphorylation	VQADESQSPRDRGRI CCCCCCCCHHHHCCC	3.86	19664994
863	Phosphorylation	SPRDRGRISAHQGAQ CHHHHCCCCHHCCCC	6.86	27251275
864	Phosphorylation	PRDRGRISAHQGAQV HHHHCCCCHHCCCCC	54.53	28348404
873	Phosphorylation	HQGAQVDSRHPQGQW HCCCCCCCCCCCCCC	61.85	27251275
884	Phosphorylation	QGQWRQDSMRTYERE CCCCCHHHHHHHHHH	46.50	24670416
893	Phosphorylation	RTYEREALKKKFMRV HHHHHHHHHHHHCCH	27.25	27251275
905	Phosphorylation	MRVHDAESSDEDGYD CCHHCCCCCCCCCCC	46.55	25159151
906	Phosphorylation	RVHDAESSDEDGYDW CHHCCCCCCCCCCCC	30.79	23927012
911	Phosphorylation	ESSDEDGYDWGPATD CCCCCCCCCCCCCCC	5.33	23663014
914	Phosphorylation	DEDGYDWGPATDL-- CCCCCCCCCCCCC--	3.00	27251275
915	Phosphorylation	EDGYDWGPATDL--- CCCCCCCCCCCC---	20.42	27251275
917	Phosphorylation	GYDWGPATDL----- CCCCCCCCCC-----	12.05	23927012
919	Phosphorylation	DWGPATDL------- CCCCCCCC-------	44.82	18669648
920	Phosphorylation	WGPATDL-------- CCCCCCC--------	36.20	18669648
925	Phosphorylation	DL------------- CC-------------	22.35	18669648

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of ZO3_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ZO3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ZO3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
INADL_HUMAN	INADL	physical	12021270
ZO1_HUMAN	TJP1	physical	10575001

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ZO3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, AND MASSSPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164; SER-919 ANDSER-920, AND MASS SPECTROMETRY.	18669648 [Abstract]