VP26A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: VP26A_MOUSE
• UniProt AC: P40336
• Protein Name: Vacuolar protein sorting-associated protein 26A
• Gene Name: Vps26a
• Organism: Mus musculus (Mouse).
• Sequence Length: 327
• Subcellular Localization: Cytoplasm . Endosome membrane ; Peripheral membrane protein . Early endosome . Predominantly found in early not late endosomes.
• Protein Description: Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3.The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC complex seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (By similarity). Required for retrograde transport of lysosomal enzyme receptor IGF2R. [PubMed: 15078902 Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA Required for the endosomal localization of WASHC2 (indicative for the WASH complex Required for the endosomal localization of TBC1D5. Mediates retromer cargo recognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP (By similarity Involved in retromer-independent lysosomal sorting of F2R. Involved in recycling of ADRB2 (By similarity Acts redundantly with VSP26B in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1. Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins]
• Protein Sequence: MSFLGGFFGPICEIDVALNDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPDSQASAEQPEM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSFLGGFFG ------CCCCCCCCC	29.22	26824392
31	Phosphorylation	RKMAEMKTEDGKVEK CEEEEEECCCCCEEE	37.69	28059163
57	Ubiquitination	GKVNLAFKQPGKRLE CEEEEEECCCCCCCE	50.40	22790023
57 (in isoform 2)	Ubiquitination	-	50.40	22790023
89 (in isoform 2)	Ubiquitination	-	5.02	-
89	Ubiquitination	KSNTHEFVNLVKELA CCCHHHHHHHHHHHC	5.02	27667366
131	Ubiquitination	ANVRLRYFLKVTIVR CCCCHHHHHHHHHHH	4.26	27667366
214	Ubiquitination	MELQLIKKEITGIGP HCHHHHHHECCCCCC	47.56	-
214 (in isoform 2)	Ubiquitination	-	47.56	22790023
214	Malonylation	MELQLIKKEITGIGP HCHHHHHHECCCCCC	47.56	26320211
242	Ubiquitination	IMDGAPVKGESIPIR EECCCCCCCCCCCEE	56.77	22790023
242 (in isoform 2)	Ubiquitination	-	56.77	22790023
246 (in isoform 2)	Ubiquitination	-	2.60	-
274	Ubiquitination	KFSVRYFLNLVLVDE HHCCEEEEEEEEECH	3.43	27667366
274 (in isoform 2)	Ubiquitination	-	3.43	-
288	Acetylation	EEDRRYFKQQEIILW HHHHHHHHHHHEEEE	41.98	22826441
307	Phosphorylation	EKLRKQRTNFHQRFE HHHHHHCCCHHHHHC	39.48	26643407
315	Phosphorylation	NFHQRFESPDSQASA CHHHHHCCCCCHHHH	31.15	25521595
318	Phosphorylation	QRFESPDSQASAEQP HHHCCCCCHHHHCCC	30.91	27742792
321	Phosphorylation	ESPDSQASAEQPEM- CCCCCHHHHCCCCC-	25.38	25521595
347	Phosphorylation	--------------------------- ---------------------------		24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of VP26A_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of VP26A_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of VP26A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
VPS29_MOUSE	Vps29	physical	18656452
VP26B_MOUSE	Vps26b	physical	18656452
VPS35_MOUSE	Vps35	physical	18656452

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.