dbPTM

US6NL_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	US6NL_HUMAN
UniProt AC	Q92738
Protein Name	USP6 N-terminal-like protein
Gene Name	USP6NL
Organism	Homo sapiens (Human).
Sequence Length	828
Subcellular Localization	Golgi apparatus. Cytoplasmic vesicle.
Protein Description	Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex..
Protein Sequence	MNSDQDVALKLAQERAEIVAKYDRGREGAEIEPWEDADYLVYKVTDRFGFLHEEELPDHNVAVERQKHLEIERTTKWLKMLKGWEKYKNTEKFHRRIYKGIPLQLRGEVWALLLEIPKMKEETRDLYSKLKHRARGCSPDIRQIDLDVNRTFRDHIMFRDRYGVKQQSLFHVLAAYSIYNTEVGYCQGMSQITALLLMYMNEEDAFWALVKLFSGPKHAMHGFFVQGFPKLLRFQEHHEKILNKFLSKLKQHLDSQEIYTSFYTMKWFFQCFLDRTPFTLNLRIWDIYIFEGERVLTAMSYTILKLHKKHLMKLSMEELVEFFQETLAKDFFFEDDFVIEQLQISMTELKRAKLDLPEPGKEDEYPKKPLGQLPPELQSWGVHHLSNGQRSVGRPSPLASGRRESGAPHRRHEHSPHPQSRTGTPERAQPPRRKSVEEESKKLKDEADFQRKLPSGPQDSSRQYNHAAANQNSNATSNIRKEFVPKWNKPSDVSATERTAKYTMEGKGRAAHPALAVTVPGPAEVRVSNVRPKMKALDAEDGKRGSTASQYDNVPGPELDSGASVEEALERAYSQSPRHALYPPSPRKHAEPSSSPSKVSNKFTFKVQPPSHARYPSQLDGEARGLAHPPSYSNPPVYHGNSPKHFPTANSSFASPQFSPGTQLNPSRRPHGSTLSVSASPEKSYSRPSPLVLPSSRIEVLPVDTGAGGYSGNSGSPKNGKLIIPPVDYLPDNRTWSEVSYTYRPETQGQSWTRDASRGNLPKYSAFQLAPFQDHGLPAVSVDSPVRYKASPAAEDASPSGYPYSGPPPPAYHYRNRDGLSIQESVLL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MNSDQDVA -------CCCHHHHH	11.06	22814378
3 (in isoform 2)	Phosphorylation	-	35.04	30631047
9 (in isoform 2)	Phosphorylation	-	4.73	30631047
10	Ubiquitination	SDQDVALKLAQERAE CHHHHHHHHHHHHHH	31.85	-
13	Phosphorylation	DVALKLAQERAEIVA HHHHHHHHHHHHHHH	51.66	24719451
13 (in isoform 2)	Phosphorylation	-	51.66	24719451
17	Phosphorylation	KLAQERAEIVAKYDR HHHHHHHHHHHHCCC	44.19	24719451
17 (in isoform 2)	Phosphorylation	-	44.19	24719451
20	Phosphorylation	QERAEIVAKYDRGRE HHHHHHHHHCCCCCC	15.45	24719451
20 (in isoform 2)	Phosphorylation	-	15.45	24719451
21	Ubiquitination	ERAEIVAKYDRGREG HHHHHHHHCCCCCCC	36.47	-
39	Phosphorylation	EPWEDADYLVYKVTD CCCCCCCEEEEEECC	10.37	28796482
42	Phosphorylation	EDADYLVYKVTDRFG CCCCEEEEEECCCCC	9.35	28796482
56	Phosphorylation	GFLHEEELPDHNVAV CCCCHHCCCCCCCCC	7.36	27642862
59	Phosphorylation	HEEELPDHNVAVERQ CHHCCCCCCCCCHHH	28.82	27642862
86	Ubiquitination	KMLKGWEKYKNTEKF HHHHCHHHHCCCHHH	55.82	-
88	Ubiquitination	LKGWEKYKNTEKFHR HHCHHHHCCCHHHHH	69.34	-
131	Ubiquitination	RDLYSKLKHRARGCS HHHHHHHHHHHCCCC	34.48	-
148 (in isoform 2)	Ubiquitination	-	7.30	-
240	Ubiquitination	RFQEHHEKILNKFLS HHHHHHHHHHHHHHH	48.41	-
255	Phosphorylation	KLKQHLDSQEIYTSF HHHHHCCCHHHHHHH	35.75	-
259	Phosphorylation	HLDSQEIYTSFYTMK HCCCHHHHHHHHHHH	8.79	29759185
260	Phosphorylation	LDSQEIYTSFYTMKW CCCHHHHHHHHHHHH	20.30	29759185
261	Phosphorylation	DSQEIYTSFYTMKWF CCHHHHHHHHHHHHH	10.17	29759185
353	Ubiquitination	MTELKRAKLDLPEPG HHHHHHHCCCCCCCC	46.71	-
361	Ubiquitination	LDLPEPGKEDEYPKK CCCCCCCCCCCCCCC	73.11	-
391	Phosphorylation	HLSNGQRSVGRPSPL ECCCCCCCCCCCCCC	22.49	30266825
396	Phosphorylation	QRSVGRPSPLASGRR CCCCCCCCCCCCCCC	31.05	30266825
400	Phosphorylation	GRPSPLASGRRESGA CCCCCCCCCCCCCCC	40.28	30266825
405	Phosphorylation	LASGRRESGAPHRRH CCCCCCCCCCCCCCC	38.14	22817900
408	Phosphorylation	GRRESGAPHRRHEHS CCCCCCCCCCCCCCC	25.66	24719451
413	Phosphorylation	GAPHRRHEHSPHPQS CCCCCCCCCCCCCCC	44.22	24719451
422	Phosphorylation	SPHPQSRTGTPERAQ CCCCCCCCCCCCCCC	51.13	24300666
424	Phosphorylation	HPQSRTGTPERAQPP CCCCCCCCCCCCCCC	22.74	24245541
435	Phosphorylation	AQPPRRKSVEEESKK CCCCCCCCHHHHHHH	32.71	26657352
440	Phosphorylation	RKSVEEESKKLKDEA CCCHHHHHHHHHHHH	38.07	28060719
452	Phosphorylation	DEADFQRKLPSGPQD HHHHHHHHCCCCCCC	54.70	24719451
455	Phosphorylation	DFQRKLPSGPQDSSR HHHHHCCCCCCCHHH	73.81	23312004
460	Phosphorylation	LPSGPQDSSRQYNHA CCCCCCCHHHHHCHH	23.36	26434776
461	Phosphorylation	PSGPQDSSRQYNHAA CCCCCCHHHHHCHHH	31.52	26434776
464	Phosphorylation	PQDSSRQYNHAAANQ CCCHHHHHCHHHHHC	14.06	24043423
473	Phosphorylation	HAAANQNSNATSNIR HHHHHCCCCCCHHHH	20.34	24043423
476	Phosphorylation	ANQNSNATSNIRKEF HHCCCCCCHHHHHHH	26.93	24043423
477	Phosphorylation	NQNSNATSNIRKEFV HCCCCCCHHHHHHHC	28.05	24043423
481	Phosphorylation	NATSNIRKEFVPKWN CCCHHHHHHHCCCCC	52.27	27642862
494	Phosphorylation	WNKPSDVSATERTAK CCCCCCCCCCHHHEE	34.17	18491316
502	Phosphorylation	ATERTAKYTMEGKGR CCHHHEEHHCCCCCC	14.62	21712546
509	Methylation	YTMEGKGRAAHPALA HHCCCCCCCCCCEEE	31.89	115919669
528	Phosphorylation	GPAEVRVSNVRPKMK CCCEEEECCCCHHCE	21.02	24719451
545	Phosphorylation	DAEDGKRGSTASQYD CCCCCCCCCCCHHCC	32.60	24719451
546	Phosphorylation	AEDGKRGSTASQYDN CCCCCCCCCCHHCCC	25.49	30266825
547	Phosphorylation	EDGKRGSTASQYDNV CCCCCCCCCHHCCCC	32.90	30266825
549	Phosphorylation	GKRGSTASQYDNVPG CCCCCCCHHCCCCCC	29.39	30266825
551	Phosphorylation	RGSTASQYDNVPGPE CCCCCHHCCCCCCCC	13.64	30266825
561	Phosphorylation	VPGPELDSGASVEEA CCCCCCCCCCCHHHH	49.21	28348404
563	Phosphorylation	GPELDSGASVEEALE CCCCCCCCCHHHHHH	17.85	27642862
564	Phosphorylation	PELDSGASVEEALER CCCCCCCCHHHHHHH	33.32	27642862
566	Phosphorylation	LDSGASVEEALERAY CCCCCCHHHHHHHHH	34.75	27642862
568	Phosphorylation	SGASVEEALERAYSQ CCCCHHHHHHHHHHC	10.96	27642862
573	Phosphorylation	EEALERAYSQSPRHA HHHHHHHHHCCCCCC	17.29	23312004
574	Phosphorylation	EALERAYSQSPRHAL HHHHHHHHCCCCCCC	24.17	29414761
576	Phosphorylation	LERAYSQSPRHALYP HHHHHHCCCCCCCCC	20.26	30576142
582	Phosphorylation	QSPRHALYPPSPRKH CCCCCCCCCCCCCCC	17.55	30266825
585	Phosphorylation	RHALYPPSPRKHAEP CCCCCCCCCCCCCCC	32.60	30266825
593	Phosphorylation	PRKHAEPSSSPSKVS CCCCCCCCCCCCCCC	34.65	25849741
594	Phosphorylation	RKHAEPSSSPSKVSN CCCCCCCCCCCCCCC	57.91	20363803
595	Phosphorylation	KHAEPSSSPSKVSNK CCCCCCCCCCCCCCC	37.21	25849741
597	Phosphorylation	AEPSSSPSKVSNKFT CCCCCCCCCCCCCEE	47.87	23312004
599	Phosphorylation	PSSSPSKVSNKFTFK CCCCCCCCCCCEEEE	10.01	27642862
600	Phosphorylation	SSSPSKVSNKFTFKV CCCCCCCCCCEEEEE	37.73	23312004
602	Methylation	SPSKVSNKFTFKVQP CCCCCCCCEEEEECC	37.97	-
602	Phosphorylation	SPSKVSNKFTFKVQP CCCCCCCCEEEEECC	37.97	24719451
604	Phosphorylation	SKVSNKFTFKVQPPS CCCCCCEEEEECCCC	24.93	23312004
611	Phosphorylation	TFKVQPPSHARYPSQ EEEECCCCCCCCHHH	36.14	24719451
615	Phosphorylation	QPPSHARYPSQLDGE CCCCCCCCHHHCCCC	14.18	27251275
617	Phosphorylation	PSHARYPSQLDGEAR CCCCCCHHHCCCCCC	34.15	17525332
631	Phosphorylation	RGLAHPPSYSNPPVY CCCCCCCCCCCCCCC	45.72	21945579
632	Phosphorylation	GLAHPPSYSNPPVYH CCCCCCCCCCCCCCC	20.45	21945579
633	Phosphorylation	LAHPPSYSNPPVYHG CCCCCCCCCCCCCCC	47.39	21945579
634	Phosphorylation	AHPPSYSNPPVYHGN CCCCCCCCCCCCCCC	36.34	24719451
638	Phosphorylation	SYSNPPVYHGNSPKH CCCCCCCCCCCCCCC	15.13	21945579
642	Phosphorylation	PPVYHGNSPKHFPTA CCCCCCCCCCCCCCC	39.66	21945579
648	Phosphorylation	NSPKHFPTANSSFAS CCCCCCCCCCCCCCC	37.21	23403867
649	Phosphorylation	SPKHFPTANSSFASP CCCCCCCCCCCCCCC	17.72	27642862
650	Phosphorylation	PKHFPTANSSFASPQ CCCCCCCCCCCCCCC	40.44	27642862
651	Phosphorylation	KHFPTANSSFASPQF CCCCCCCCCCCCCCC	25.11	23403867
652	Phosphorylation	HFPTANSSFASPQFS CCCCCCCCCCCCCCC	25.33	23403867
655	Phosphorylation	TANSSFASPQFSPGT CCCCCCCCCCCCCCC	19.75	25159151
659	Phosphorylation	SFASPQFSPGTQLNP CCCCCCCCCCCCCCC	19.71	25159151
662	Phosphorylation	SPQFSPGTQLNPSRR CCCCCCCCCCCCCCC	33.01	23403867
668	Phosphorylation	GTQLNPSRRPHGSTL CCCCCCCCCCCCCCE	58.36	24719451
673	Phosphorylation	PSRRPHGSTLSVSAS CCCCCCCCCEEEECC	24.12	23403867
674	Phosphorylation	SRRPHGSTLSVSASP CCCCCCCCEEEECCC	27.87	23403867
676	Phosphorylation	RPHGSTLSVSASPEK CCCCCCEEEECCCCC	17.70	30266825
678	Phosphorylation	HGSTLSVSASPEKSY CCCCEEEECCCCCCC	21.47	30266825
680	Phosphorylation	STLSVSASPEKSYSR CCEEEECCCCCCCCC	26.53	30266825
684	Phosphorylation	VSASPEKSYSRPSPL EECCCCCCCCCCCCC	27.03	30576142
685	Phosphorylation	SASPEKSYSRPSPLV ECCCCCCCCCCCCCC	21.55	30576142
686	Phosphorylation	ASPEKSYSRPSPLVL CCCCCCCCCCCCCCC	44.82	23312004
689	Phosphorylation	EKSYSRPSPLVLPSS CCCCCCCCCCCCCCC	29.74	30576142
690	Phosphorylation	KSYSRPSPLVLPSSR CCCCCCCCCCCCCCC	29.21	24719451
691	Phosphorylation	SYSRPSPLVLPSSRI CCCCCCCCCCCCCCE	7.88	24719451
695	Phosphorylation	PSPLVLPSSRIEVLP CCCCCCCCCCEEEEE	28.25	29083192
696	Phosphorylation	SPLVLPSSRIEVLPV CCCCCCCCCEEEEEE	35.02	29083192
697	Phosphorylation	PLVLPSSRIEVLPVD CCCCCCCCEEEEEEC	33.16	24719451
705	Phosphorylation	IEVLPVDTGAGGYSG EEEEEECCCCCCCCC	29.21	21945579
706	Phosphorylation	EVLPVDTGAGGYSGN EEEEECCCCCCCCCC	20.12	24719451
710	Phosphorylation	VDTGAGGYSGNSGSP ECCCCCCCCCCCCCC	16.70	21945579
711	Phosphorylation	DTGAGGYSGNSGSPK CCCCCCCCCCCCCCC	34.47	21945579
714	Phosphorylation	AGGYSGNSGSPKNGK CCCCCCCCCCCCCCC	44.14	29255136
716	Phosphorylation	GYSGNSGSPKNGKLI CCCCCCCCCCCCCEE	32.30	29255136
722	Phosphorylation	GSPKNGKLIIPPVDY CCCCCCCEECCCCCC	4.56	27251275
727	Phosphorylation	GKLIIPPVDYLPDNR CCEECCCCCCCCCCC	6.75	27642862
728	Phosphorylation	KLIIPPVDYLPDNRT CEECCCCCCCCCCCC	45.05	27642862
729	Phosphorylation	LIIPPVDYLPDNRTW EECCCCCCCCCCCCC	21.99	14729942
731	Phosphorylation	IPPVDYLPDNRTWSE CCCCCCCCCCCCCEE	30.51	27642862
733	Phosphorylation	PVDYLPDNRTWSEVS CCCCCCCCCCCEECE	41.14	24719451
740	Phosphorylation	NRTWSEVSYTYRPET CCCCEECEEEECCCC	14.01	28348404
741	Phosphorylation	RTWSEVSYTYRPETQ CCCEECEEEECCCCC	16.99	22817900
742	Phosphorylation	TWSEVSYTYRPETQG CCEECEEEECCCCCC	12.92	28348404
743	Phosphorylation	WSEVSYTYRPETQGQ CEECEEEECCCCCCC	18.88	22817900
746	Phosphorylation	VSYTYRPETQGQSWT CEEEECCCCCCCCCC	44.77	27642862
747	Phosphorylation	SYTYRPETQGQSWTR EEEECCCCCCCCCCC	40.45	24719451
751	Phosphorylation	RPETQGQSWTRDASR CCCCCCCCCCCCCCC	36.92	27251275
753	Phosphorylation	ETQGQSWTRDASRGN CCCCCCCCCCCCCCC	24.74	30576142
764	Phosphorylation	SRGNLPKYSAFQLAP CCCCCCCCCEEEECC	11.86	24732914
765	Phosphorylation	RGNLPKYSAFQLAPF CCCCCCCCEEEECCC	28.82	24732914
781	Phosphorylation	DHGLPAVSVDSPVRY CCCCCCEECCCCCCC	23.47	24732914
784	Phosphorylation	LPAVSVDSPVRYKAS CCCEECCCCCCCCCC	24.12	25159151
788	Phosphorylation	SVDSPVRYKASPAAE ECCCCCCCCCCCCCC	15.90	24732914
791	Phosphorylation	SPVRYKASPAAEDAS CCCCCCCCCCCCCCC	15.77	21945579
798	Phosphorylation	SPAAEDASPSGYPYS CCCCCCCCCCCCCCC	31.74	21945579
800	Phosphorylation	AAEDASPSGYPYSGP CCCCCCCCCCCCCCC	48.76	21945579
801	Phosphorylation	AEDASPSGYPYSGPP CCCCCCCCCCCCCCC	30.71	24719451
802	Phosphorylation	EDASPSGYPYSGPPP CCCCCCCCCCCCCCC	11.88	21945579
804	Phosphorylation	ASPSGYPYSGPPPPA CCCCCCCCCCCCCCC	20.13	21945579
805	Phosphorylation	SPSGYPYSGPPPPAY CCCCCCCCCCCCCCC	40.08	21945579
808	Phosphorylation	GYPYSGPPPPAYHYR CCCCCCCCCCCCCCC	51.54	24719451
812	Phosphorylation	SGPPPPAYHYRNRDG CCCCCCCCCCCCCCC	12.73	21945579
814	Phosphorylation	PPPPAYHYRNRDGLS CCCCCCCCCCCCCCC	9.26	21945579
815	Phosphorylation	PPPAYHYRNRDGLSI CCCCCCCCCCCCCCC	20.56	27642862
817	Phosphorylation	PAYHYRNRDGLSIQE CCCCCCCCCCCCCCC	30.31	27251275
819	Phosphorylation	YHYRNRDGLSIQESV CCCCCCCCCCCCCCC	20.35	27642862
821	Phosphorylation	YRNRDGLSIQESVLL CCCCCCCCCCCCCCC	27.96	21609022
822	Phosphorylation	RNRDGLSIQESVLL- CCCCCCCCCCCCCC-	6.74	27642862
829	Phosphorylation	IQESVLL-------- CCCCCCC--------		27642862
831	Phosphorylation	ESVLL---------- CCCCC----------		27642862

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of US6NL_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of US6NL_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of US6NL_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
ACTN4_HUMAN	ACTN4	physical	15152255
A4_HUMAN	APP	physical	21832049

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of US6NL_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391; SER-396 ANDSER-716, AND MASS SPECTROMETRY.	18669648 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-617, AND MASSSPECTROMETRY.	17525332 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, AND MASSSPECTROMETRY.	17081983 [Abstract]
"Robust phosphoproteomic profiling of tyrosine phosphorylation sitesfrom human T cells using immobilized metal affinity chromatography andtandem mass spectrometry."; Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M.,Peters E.C.; Anal. Chem. 76:2763-2772(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-582; SER-585; TYR-710;SER-716 AND TYR-729, AND MASS SPECTROMETRY.	15144186 [Abstract]