TRM5_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TRM5_YEAST
• UniProt AC: P38793
• Protein Name: tRNA (guanine(37)-N1)-methyltransferase {ECO:0000255|HAMAP-Rule:MF_03152}
• Gene Name: TRM5 {ECO:0000255|HAMAP-Rule:MF_03152}
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 499
• Subcellular Localization: Mitochondrion matrix. Nucleus. Cytoplasm. Predominantly in the mitochondria and in the nucleus.
• Protein Description: Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding. Postspliced cytoplasmic tRNAs are imported into the nucleus, where this first step seems to take place, after which they are reexported to the cytoplasm, where the yW sythesis is completed by cytoplasmic enzymes..
• Protein Sequence: MKIALPVFQKFNRLISSCKMSGVFPYNPPVNRQMRELDRSFFITKIPMCAVKFPEPKNISVFSKNFKNCILRVPRIPHVVKLNSSKPKDELTSVQNKKLKTADGNNTPVTKGVLLHESIHSVEDAYGKLPEDALAFLKENSAEIVPHEYVLDYDFWKAEEILRAVLPEQFLEEVPTGFTITGHIAHLNLRTEFKPFDSLIGQVILDKNNKIECVVDKVSSIATQFRTFPMKVIAGKSDSLVVEQKESNCTFKFDFSKVYWNSRLHTEHERLVKQYFQPGQVVCDVFAGVGPFAVPAGKKDVIVLANDLNPESYKYLKENIALNKVAKTVKSFNMDGADFIRQSPQLLQQWIQDEEGGKITIPLPLKKRHRSQQHNDQQPPQPRTKELIIPSHISHYVMNLPDSAISFLGNFRGIFAAHTKGATDTIQMPWVHVHCFEKYPPGDQVTEDELHARVHARIIAALKVTADDLPLNAVSLHLVRKVAPTKPMYCASFQLPANV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
64	Acetylation	KNISVFSKNFKNCIL CCCEEECCCCCCCEE	55.74	24489116
92	Phosphorylation	SKPKDELTSVQNKKL CCCHHHCHHHHCCCC	25.46	30377154
97	Acetylation	ELTSVQNKKLKTADG HCHHHHCCCCCCCCC	42.25	25381059
107	Phosphorylation	KTADGNNTPVTKGVL CCCCCCCCCCHHHHH	24.12	27214570
236	Ubiquitination	PMKVIAGKSDSLVVE CEEEEECCCCCEEEE	41.91	23749301
239	Phosphorylation	VIAGKSDSLVVEQKE EEECCCCCEEEEECC	30.10	30377154
257	Acetylation	TFKFDFSKVYWNSRL EEEEEEHHHEECCCC	39.06	24489116
371	Phosphorylation	PLKKRHRSQQHNDQQ CCCHHCCHHCCCCCC	28.24	28889911

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TRM5_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TRM5_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TRM5_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KPC1_YEAST	PKC1	physical	16554755
VPS35_YEAST	VPS35	physical	16554755

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.