dbPTM

SKA2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SKA2_HUMAN
UniProt AC	Q8WVK7
Protein Name	Spindle and kinetochore-associated protein 2
Gene Name	SKA2
Organism	Homo sapiens (Human).
Sequence Length	121
Subcellular Localization	Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated
Protein Description	Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. [PubMed: 17093495]
Protein Sequence	MEAEVDKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTLYARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEEKTAAEQFKFHMPDL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
21	Phosphorylation	KAESDLDYIQYRLEY HHHHCCHHHHHHHHE	9.38	27642862
24	Phosphorylation	SDLDYIQYRLEYEIK HCCHHHHHHHHEEHH	13.96	27642862
31	Ubiquitination	YRLEYEIKTNHPDSA HHHHEEHHCCCCCCC	31.19	-
41	Ubiquitination	HPDSASEKNPVTLLK CCCCCCCCCCCHHHH	65.15	-
62 (in isoform 2)	Phosphorylation	-	17.34	-
65	Ubiquitination	QTLYARFKPVAVEQK HHHHHHHCCEEEECC	32.79	-
72	Ubiquitination	KPVAVEQKESKSRIC CCEEEECCCCCCHHH	50.91	-
83	Acetylation	SRICATVKKTMNMIQ CHHHHHHHHHHHHHH	37.87	25953088
85	Phosphorylation	ICATVKKTMNMIQKL HHHHHHHHHHHHHHH	14.20	30631047
94	Ubiquitination	NMIQKLQKQTDLELS HHHHHHHHHCCCCCC	66.65	-
96	Phosphorylation	IQKLQKQTDLELSPL HHHHHHHCCCCCCCC	49.97	23403867
101	Phosphorylation	KQTDLELSPLTKEEK HHCCCCCCCCCHHHH	14.42	30266825
104	Phosphorylation	DLELSPLTKEEKTAA CCCCCCCCHHHHHHH	39.53	30266825
105	Ubiquitination	LELSPLTKEEKTAAE CCCCCCCHHHHHHHH	72.12	-
115	Ubiquitination	KTAAEQFKFHMPDL- HHHHHHHCCCCCCC-	33.77	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SKA2_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SKA2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SKA2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DSN1_HUMAN	DSN1	physical	22371557
SPC24_HUMAN	SPC24	physical	22371557
SKA1_HUMAN	SKA1	physical	17093495

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SKA2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, AND MASSSPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, AND MASSSPECTROMETRY.	19413330 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, AND MASSSPECTROMETRY.	18691976 [Abstract]
"Evaluation of the low-specificity protease elastase for large-scalephosphoproteome analysis."; Wang B., Malik R., Nigg E.A., Korner R.; Anal. Chem. 80:9526-9533(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, AND MASSSPECTROMETRY.	19007248 [Abstract]