SIAH2_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SIAH2_RAT
• UniProt AC: Q8R4T2
• Protein Name: E3 ubiquitin-protein ligase SIAH2
• Gene Name: Siah2
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 325
• Subcellular Localization: Cytoplasm . Nucleus . Predominantly cytoplasmic. Partially nuclear.
• Protein Description: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. [PubMed: 11786535 E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates]
• Protein Sequence: MSRPSSTGPSANKPCSKQPPPPQTPHAPSPAAPPAAATISAAGPGSSAVPAAAAVISGPGAGGGAGPVSPQHHELTSLFECPVCFDYVLPPILQCQAGHLVCNQCRQKLSCCPTCRGALTPSIRNLAMEKVASAVLFPCKYATTGCSLTLHHTEKPEHEDICEYRPYSCPCPGASCKWQGSLEAVMSHLMHAHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGHHFMLVLEKQEKYEGHQQFFAIVLLIGTRKQAENFAYRLELNGNRRRLTWEATPRSIHDGVAAAIMNSDCLVFDTAIAHLFADNGNLGINVTISTCCQ

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
6	Phosphorylation	--MSRPSSTGPSANK --CCCCCCCCCCCCC	39.81	-
16	Phosphorylation	PSANKPCSKQPPPPQ CCCCCCCCCCCCCCC	42.74	-
24	Phosphorylation	KQPPPPQTPHAPSPA CCCCCCCCCCCCCCC	23.86	-
29	Phosphorylation	PQTPHAPSPAAPPAA CCCCCCCCCCCCCCE	28.14	-
69	Phosphorylation	GGGAGPVSPQHHELT CCCCCCCCCCHHHCC	23.21	-
120	Phosphorylation	PTCRGALTPSIRNLA CCCCCCCCHHHHHHH	17.92	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
16	S	Phosphorylation	Kinase	DYRK2	-	Uniprot
24	T	Phosphorylation	Kinase	MAPK14	P70618	Uniprot
29	S	Phosphorylation	Kinase	MAPK14	P70618	Uniprot
29	S	Phosphorylation	Kinase	DYRK2	-	Uniprot
69	S	Phosphorylation	Kinase	DYRK2	-	Uniprot
120	T	Phosphorylation	Kinase	DYRK2	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
24	T	Phosphorylation	Phosphorylated at Thr-24 and Ser-29 by MAPK14, which mediates the degradation by the proteasome of EGLN3.	-
29	S	Phosphorylation	Phosphorylated at Ser-29 by DYRK2; this increases the ubiquitin ligase activity and promotes degradation of EGLN3 (By similarity).	-
29	S	Phosphorylation	Phosphorylated at Thr-24 and Ser-29 by MAPK14, which mediates the degradation by the proteasome of EGLN3.	-
29	S	ubiquitylation	Phosphorylated at Ser-29 by DYRK2; this increases the ubiquitin ligase activity and promotes degradation of EGLN3 (By similarity).	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SIAH2_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SYUA_RAT	Snca	physical	19224863
SYPH_RAT	Syp	physical	11786535

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.