RPGF3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RPGF3_HUMAN
• UniProt AC: O95398
• Protein Name: Rap guanine nucleotide exchange factor 3
• Gene Name: RAPGEF3
• Organism: Homo sapiens (Human).
• Sequence Length: 923
• Subcellular Localization: Endomembrane system .
• Protein Description: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin..
• Protein Sequence: MKVGWPGESCWQVGLAVEDSPALGAPRVGALPDVVPEGTLLNMVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGRQLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVRTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
79	Phosphorylation	RWTPLTNSEESLDFS EEECCCCCHHHCCCC	37.19	-
82	Phosphorylation	PLTNSEESLDFSESL CCCCCHHHCCCCHHH	29.23	-
108 (in isoform 3)	Phosphorylation	-	3.34	-
150	Phosphorylation	GLGVHSRSQVVGICQ CCCCCCHHHHHHHHH	30.27	-
209	Phosphorylation	AEAVALLSQRGPDAL HHHHHHHHHHCCCEE	20.77	24719451
225	Phosphorylation	TVALRKPPGQRTDEE EEEECCCCCCCCHHH	55.36	-
267	Phosphorylation	VLLFEPHSKAGTVLF HHHCCCCCCCCEEEE	34.61	25159151
268	Acetylation	LLFEPHSKAGTVLFS HHCCCCCCCCEEEEE	48.04	19823561
338	Phosphorylation	NCHFLRVDKQDFNRI CCEEEECCHHHHHHH	36.14	-
370	Phosphorylation	VLVLERASQGAGPSR EEEEECCCCCCCCCC	35.05	23312004
376	Phosphorylation	ASQGAGPSRPPTPGR CCCCCCCCCCCCCCC	57.40	28857561
380	Phosphorylation	AGPSRPPTPGRNRYT CCCCCCCCCCCCCEE	40.85	23312004
452	Phosphorylation	EPAGGSEQERSTYVC CCCCCCHHHHCEEEC	53.77	-
486	Phosphorylation	MLHTDPVATSFLQKL HHCCCHHHHHHHHHH	11.69	-
494	Phosphorylation	TSFLQKLSDLVGRDT HHHHHHHHHHHCCHH	35.61	23312004
504	Phosphorylation	VGRDTRLSNLLREQW HCCHHHHHHHHHHHC	23.34	29759185
528	Phosphorylation	ENGCGNASPQMKARN CCCCCCCCCCHHHCC	21.47	23403867
822	Phosphorylation	MPLLLKDMTFIHEGN HHHHHCCCEEEECCC	2.93	-
864	Phosphorylation	SHNPVPLSPLRSRVS HCCCCCCCHHHHHCH	18.80	28355574
915	Phosphorylation	IDNQRELSRLSRELE HCCHHHHHHHHHHCC	26.82	29978859
918	Phosphorylation	QRELSRLSRELEP-- HHHHHHHHHHCCC--	23.69	29978859

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
108	S	Phosphorylation	Kinase	GRK2	P25098	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RPGF3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RPGF3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAP2A_HUMAN	RAP2A	physical	10777494
RAP1A_HUMAN	RAP1A	physical	10777494
RBP2_HUMAN	RANBP2	physical	21670213
RBP2_HUMAN	RANBP2	physical	20547757
NU205_HUMAN	NUP205	physical	20547757
NUP98_HUMAN	NUP98	physical	20547757
IMB1_HUMAN	KPNB1	physical	20547757
RAN_HUMAN	RAN	physical	20547757

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.