ROK1_YEAST - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ROK1_YEAST
• UniProt AC: P45818
• Protein Name: ATP-dependent RNA helicase ROK1
• Gene Name: ROK1
• Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
• Sequence Length: 564
• Subcellular Localization: Nucleus, nucleolus .
• Protein Description: ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity..
• Protein Sequence: MDIFRVLTRGASVKKESGPKAKAADYSVINGNDENHKEDNNESQIVKELDFFRNKRIISKVEDDREKTTENDSPNKEEKSGNDDGLIKPVITNTVEASALRKSYKGNVSGIDIPLPIGSFEDLISRFSFDKRLLNNLIENGFTEPTPIQCECIPVALNNRDVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSYKIFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKHLIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRVIIGHKEAANTNIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHELMYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVPGSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMAKMTRKEKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
26	Phosphorylation	PKAKAADYSVINGND CCCCCCCEEEECCCC	10.50	21440633
37	Acetylation	NGNDENHKEDNNESQ CCCCCCCCCCCCHHH	77.63	24489116
59	Phosphorylation	FRNKRIISKVEDDRE HHCCHHHEECCCCHH	28.42	25704821
60	Acetylation	RNKRIISKVEDDREK HCCHHHEECCCCHHH	38.95	22865919
73	Phosphorylation	EKTTENDSPNKEEKS HHCCCCCCCCHHHHC	41.79	24603354
80	Phosphorylation	SPNKEEKSGNDDGLI CCCHHHHCCCCCCCC	46.22	28889911
88	Acetylation	GNDDGLIKPVITNTV CCCCCCCCHHCCCCH	37.97	24489116
199	Phosphorylation	GLKGLIISPTKELAN CCCCEEECCCHHHHH	21.37	27717283
218	Acetylation	ECFKLSYKIFLEKKR HHHHHHHHHHHHCCC	24.49	24489116
223	Acetylation	SYKIFLEKKRPLQVA HHHHHHHCCCHHHHH	57.12	24489116
239	Acetylation	LSKSLGAKLKNKVVS HHHHHCHHHHCCCCC	59.19	25381059
249	Acetylation	NKVVSDKKYDIIIST CCCCCCCCCEEEEEC	53.82	24489116
265	Acetylation	LRLIDVVKNEALDLS HHHHHHHCCCCCCHH	49.25	24489116
314	Phosphorylation	RKAMFSATIPSNVEE HHHHHHCCCCCCHHH	32.02	27017623
317	Phosphorylation	MFSATIPSNVEEIAQ HHHCCCCCCHHHHHH	50.05	27017623
550	Acetylation	QEMIAASKRRKNEEL HHHHHHHHHHHHHHH	52.04	25381059

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of ROK1_YEAST !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ROK1_YEAST !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ROK1_YEAST !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RS3A2_YEAST	RPS1B	physical	16429126
RS24A_YEAST	RPS24B	physical	16429126
RS24B_YEAST	RPS24B	physical	16429126
RL4A_YEAST	RPL4A	physical	16429126
MPP10_YEAST	MPP10	physical	16449634
DCA13_YEAST	SOF1	genetic	27708008
RL1D1_YEAST	UTP30	genetic	27708008
RRP5_YEAST	RRP5	physical	27280440

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.