RASK_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RASK_RAT
• UniProt AC: P08644
• Protein Name: GTPase KRas
• Gene Name: Kras
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 189
• Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytosol .
• Protein Description: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (By similarity). Plays an important role in the regulation of cell proliferation. [PubMed: 3110778 Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (By similarity]
• Protein Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQELARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKCVIM

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MTEYKLVV -------CCCEEEEE	6.07	-
2	Acetylation	------MTEYKLVVV ------CCCEEEEEE	48.28	-
39	Phosphorylation	YDPTIEDSYRKQVVI CCCCCCHHHCCEEEE	17.66	30181290
41	Dimethylation	PTIEDSYRKQVVIDG CCCCHHHCCEEEECC	27.21	-
41	Methylation	PTIEDSYRKQVVIDG CCCCHHHCCEEEECC	27.21	4204345
42	"N6,N6-dimethyllysine"	TIEDSYRKQVVIDGE CCCHHHCCEEEECCC	38.93	-
42	Methylation	TIEDSYRKQVVIDGE CCCHHHCCEEEECCC	38.93	12692561
104	Acetylation	REQIKRVKDSEDVPM HHHHHCCCCCCCCCE	60.62	-
118	S-nitrosocysteine	MVLVGNKCDLPSRTV EEEECCCCCCCCCCC	8.24	-
128	Ubiquitination	PSRTVDTKQAQELAR CCCCCCHHHHHHHHH	38.89	-
180	S-palmitoylation	KEEKTPGCVKIKKCV CCCCCCCCEEEEEEE	2.72	-
186	Methylation	GCVKIKKCVIM---- CCEEEEEEEEC----	1.81	-
186	Farnesylation	GCVKIKKCVIM---- CCEEEEEEEEC----	1.81	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RASK_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
104	K	Acetylation	Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).	-
170	K	ubiquitylation	Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RASK_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDC27_RAT	Cdc27	physical	22045811
CDC20_RAT	Cdc20	physical	22045811

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.