dbPTM

RASA3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	RASA3_HUMAN
UniProt AC	Q14644
Protein Name	Ras GTPase-activating protein 3
Gene Name	RASA3
Organism	Homo sapiens (Human).
Sequence Length	834
Subcellular Localization	Cell membrane.
Protein Description	Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor..
Protein Sequence	MAVEDEGLRVFQSVKIKIGEAKNLPSYPGPSKMRDCYCTVNLDQEEVFRTKIVEKSLCPFYGEDFYCEIPRSFRHLSFYIFDRDVFRRDSIIGKVAIQKEDLQKYHNRDTWFQLQHVDADSEVQGKVHLELRLSEVITDTGVVCHKLATRIVECQGLPIVNGQCDPYATVTLAGPFRSEAKKTKVKRKTNNPQFDEVFYFEVTRPCSYSKKSHFDFEEEDVDKLEIRVDLWNASNLKFGDEFLGELRIPLKVLRQSSSYEAWYFLQPRDNGSKSLKPDDLGSLRLNVVYTEDHVFSSDYYSPLRDLLLKSADVEPVSASAAHILGEVCREKQEAAVPLVRLFLHYGRVVPFISAIASAEVKRTQDPNTIFRGNSLASKCIDETMKLAGMHYLHVTLKPAIEEICQSHKPCEIDPVKLKDGENLENNMENLRQYVDRVFHAITESGVSCPTVMCDIFFSLREAAAKRFQDDPDVRYTAVSSFIFLRFFAPAILSPNLFQLTPHHTDPQTSRTLTLISKTVQTLGSLSKSKSASFKESYMATFYEFFNEQKYADAVKNFLDLISSSGRRDPKSVEQPIVLKEGFMIKRAQGRKRFGMKNFKKRWFRLTNHEFTYHKSKGDQPLYSIPIENILAVEKLEEESFKMKNMFQVIQPERALYIQANNCVEAKDWIDILTKVSQCNQKRLTVYHPSAYLSGHWLCCRAPSDSAPGCSPCTGGLPANIQLDIDGDRETERIYSLFNLYMSKLEKMQEACGSKSVYDGPEQEEYSTFVIDDPQETYKTLKQVIAGVGALEQEHAQYKRDKFKKTKYGSQEHPIGDKSFQNYIRQQSETSTHSI

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAVEDEGLR ------CCCCCCCCE	17.26	25489052
13	Phosphorylation	EGLRVFQSVKIKIGE CCCEEEEEEEEEECC	17.57	27251275
15	Ubiquitination	LRVFQSVKIKIGEAK CEEEEEEEEEECCCC	43.45	29967540
22	Malonylation	KIKIGEAKNLPSYPG EEEECCCCCCCCCCC	55.50	26320211
26	Phosphorylation	GEAKNLPSYPGPSKM CCCCCCCCCCCCCCC	47.31	26074081
27	Phosphorylation	EAKNLPSYPGPSKMR CCCCCCCCCCCCCCC	15.40	26074081
31	Phosphorylation	LPSYPGPSKMRDCYC CCCCCCCCCCCCEEE	45.03	26074081
32	Ubiquitination	PSYPGPSKMRDCYCT CCCCCCCCCCCEEEE	40.88	29967540
37	Phosphorylation	PSKMRDCYCTVNLDQ CCCCCCEEEEECCCH	8.74	26074081
39	Phosphorylation	KMRDCYCTVNLDQEE CCCCEEEEECCCHHH	5.97	26074081
50	Phosphorylation	DQEEVFRTKIVEKSL CHHHHHHHHHHHHHC	17.41	26074081
55	Ubiquitination	FRTKIVEKSLCPFYG HHHHHHHHHCCCCCC	37.52	-
56	Phosphorylation	RTKIVEKSLCPFYGE HHHHHHHHCCCCCCC	23.00	26552605
61	Phosphorylation	EKSLCPFYGEDFYCE HHHCCCCCCCCCEEE	12.54	28796482
66	Phosphorylation	PFYGEDFYCEIPRSF CCCCCCCEEECCCHH	11.07	28796482
67	Ubiquitination	FYGEDFYCEIPRSFR CCCCCCEEECCCHHH	3.74	29967540
72	Ubiquitination	FYCEIPRSFRHLSFY CEEECCCHHHCEEEE	22.68	-
72	Ubiquitination	FYCEIPRSFRHLSFY CEEECCCHHHCEEEE	22.68	29967540
77	Phosphorylation	PRSFRHLSFYIFDRD CCHHHCEEEEEECCH	15.18	19060867
79	Phosphorylation	SFRHLSFYIFDRDVF HHHCEEEEEECCHHH	9.41	23312004
90	Phosphorylation	RDVFRRDSIIGKVAI CHHHCCCCCEEEEEE	17.81	30266825
99	Ubiquitination	IGKVAIQKEDLQKYH EEEEEEEHHHHHHHH	47.27	29967540
104	Ubiquitination	IQKEDLQKYHNRDTW EEHHHHHHHHCCCCE	56.63	29967540
110	Phosphorylation	QKYHNRDTWFQLQHV HHHHCCCCEEEEEEC	25.58	28857561
114	Ubiquitination	NRDTWFQLQHVDADS CCCCEEEEEECCCCC	2.50	-
144	Ubiquitination	ITDTGVVCHKLATRI CCCCCHHHHHHCCHH	1.86	29967540
146	Ubiquitination	DTGVVCHKLATRIVE CCCHHHHHHCCHHHC	34.39	-
175	Phosphorylation	ATVTLAGPFRSEAKK EEEEEECCCCCHHHC	18.83	32142685
187	Ubiquitination	AKKTKVKRKTNNPQF HHCCCCCCCCCCCCC	54.76	29967540
196	Ubiquitination	TNNPQFDEVFYFEVT CCCCCCCEEEEEEEC	35.50	29967540
199	Phosphorylation	PQFDEVFYFEVTRPC CCCCEEEEEEECCCC	12.19	26074081
203	Phosphorylation	EVFYFEVTRPCSYSK EEEEEEECCCCCCCC	22.99	26074081
205	Ubiquitination	FYFEVTRPCSYSKKS EEEEECCCCCCCCCC	11.31	-
207	Phosphorylation	FEVTRPCSYSKKSHF EEECCCCCCCCCCCC	34.81	28387310
208	Phosphorylation	EVTRPCSYSKKSHFD EECCCCCCCCCCCCC	31.09	26074081
209	Phosphorylation	VTRPCSYSKKSHFDF ECCCCCCCCCCCCCC	19.81	28387310
219	Ubiquitination	SHFDFEEEDVDKLEI CCCCCCHHHCCEEEE	57.02	-
223	Acetylation	FEEEDVDKLEIRVDL CCHHHCCEEEEEEEE	48.11	20167786
233	Ubiquitination	IRVDLWNASNLKFGD EEEEEECHHCCCCCC	6.55	29967540
237	Ubiquitination	LWNASNLKFGDEFLG EECHHCCCCCCHHHH	52.10	-
244	Ubiquitination	KFGDEFLGELRIPLK CCCCHHHHHCCCCHH	36.89	-
251	Ubiquitination	GELRIPLKVLRQSSS HHCCCCHHHHHCCCC	33.59	29967540
257	Phosphorylation	LKVLRQSSSYEAWYF HHHHHCCCCCEEEEE	28.71	-
258	Ubiquitination	KVLRQSSSYEAWYFL HHHHCCCCCEEEEEE	32.04	23000965
260	Ubiquitination	LRQSSSYEAWYFLQP HHCCCCCEEEEEECC	34.37	23000965
273	Ubiquitination	QPRDNGSKSLKPDDL CCCCCCCCCCCHHHC	62.29	-
276	Ubiquitination	DNGSKSLKPDDLGSL CCCCCCCCHHHCCCE	54.19	-
277	Ubiquitination	NGSKSLKPDDLGSLR CCCCCCCHHHCCCEE	45.71	-
277	Ubiquitination	NGSKSLKPDDLGSLR CCCCCCCHHHCCCEE	45.71	29967540
282	Phosphorylation	LKPDDLGSLRLNVVY CCHHHCCCEEEEEEE	20.01	28060719
291	Ubiquitination	RLNVVYTEDHVFSSD EEEEEECCCCCCCCC	28.56	29967540
309	Ubiquitination	PLRDLLLKSADVEPV HHHHHHHHCCCCCCC	43.50	21906983
345	Phosphorylation	LVRLFLHYGRVVPFI HHHHHHHHCCHHHHH	14.58	-
346	Ubiquitination	VRLFLHYGRVVPFIS HHHHHHHCCHHHHHH	12.73	-
346	Ubiquitination	VRLFLHYGRVVPFIS HHHHHHHCCHHHHHH	12.73	29967540
363	Ubiquitination	ASAEVKRTQDPNTIF HCCCCCCCCCCCCCC	31.44	29967540
378	Ubiquitination	RGNSLASKCIDETMK CCCHHHHHHHHHHHH	29.60	21906983
395	Ubiquitination	GMHYLHVTLKPAIEE CCEEEEEEEHHHHHH	20.51	29967540
398	Ubiquitination	YLHVTLKPAIEEICQ EEEEEEHHHHHHHHH	41.40	29967540
423	Ubiquitination	KLKDGENLENNMENL CCCCCCCHHHHHHHH	6.93	29967540
426	Phosphorylation	DGENLENNMENLRQY CCCCHHHHHHHHHHH	29.75	32645325
434	Ubiquitination	MENLRQYVDRVFHAI HHHHHHHHHHHHHHH	2.61	29967540
450	Phosphorylation	ESGVSCPTVMCDIFF HHCCCCCHHHHHHHH	25.79	-
495	Ubiquitination	APAILSPNLFQLTPH HHHHHCCCHHHCCCC	50.54	29967540
511	Phosphorylation	TDPQTSRTLTLISKT CCCCCHHHHHHHHHH	24.70	-
518	Phosphorylation	TLTLISKTVQTLGSL HHHHHHHHHHHHHHH	15.85	21406692
521	Phosphorylation	LISKTVQTLGSLSKS HHHHHHHHHHHHCCC	28.84	21406692
524	Phosphorylation	KTVQTLGSLSKSKSA HHHHHHHHHCCCCCH	32.36	21406692
526	Phosphorylation	VQTLGSLSKSKSASF HHHHHHHCCCCCHHH	36.26	21406692
527	Acetylation	QTLGSLSKSKSASFK HHHHHHCCCCCHHHH	68.17	12436393
527	Ubiquitination	QTLGSLSKSKSASFK HHHHHHCCCCCHHHH	68.17	29967540
528	Phosphorylation	TLGSLSKSKSASFKE HHHHHCCCCCHHHHH	28.59	28060719
529	Acetylation	LGSLSKSKSASFKES HHHHCCCCCHHHHHH	54.50	12436403
530	Phosphorylation	GSLSKSKSASFKESY HHHCCCCCHHHHHHH	35.91	27251275
532	Phosphorylation	LSKSKSASFKESYMA HCCCCCHHHHHHHHH	43.90	27251275
536	Phosphorylation	KSASFKESYMATFYE CCHHHHHHHHHHHHH	22.31	24719451
538	Ubiquitination	ASFKESYMATFYEFF HHHHHHHHHHHHHHH	3.84	29967540
547	Ubiquitination	TFYEFFNEQKYADAV HHHHHHCHHHHHHHH	43.20	29967540
555	Ubiquitination	QKYADAVKNFLDLIS HHHHHHHHHHHHHHH	43.64	-
562	Phosphorylation	KNFLDLISSSGRRDP HHHHHHHHCCCCCCC	26.25	21406692
563	Phosphorylation	NFLDLISSSGRRDPK HHHHHHHCCCCCCCC	29.80	21406692
564	Phosphorylation	FLDLISSSGRRDPKS HHHHHHCCCCCCCCC	29.54	21406692
570	Ubiquitination	SSGRRDPKSVEQPIV CCCCCCCCCCCCCEE	72.00	29967540
579	Ubiquitination	VEQPIVLKEGFMIKR CCCCEEEECCEEEEE	44.92	29967540
584	Ubiquitination	VLKEGFMIKRAQGRK EEECCEEEEECCCCC	2.26	29967540
596	Acetylation	GRKRFGMKNFKKRWF CCCCCCCCCCHHHHH	60.66	7684887
599	Acetylation	RFGMKNFKKRWFRLT CCCCCCCHHHHHHCC	51.31	7684897
602	Ubiquitination	MKNFKKRWFRLTNHE CCCCHHHHHHCCCCE	7.50	29967540
609	Ubiquitination	WFRLTNHEFTYHKSK HHHCCCCEEEEECCC	41.58	-
609	Ubiquitination	WFRLTNHEFTYHKSK HHHCCCCEEEEECCC	41.58	23000965
611	Ubiquitination	RLTNHEFTYHKSKGD HCCCCEEEEECCCCC	23.02	23000965
616	Ubiquitination	EFTYHKSKGDQPLYS EEEEECCCCCCCCEE	71.90	29967540
634	Ubiquitination	ENILAVEKLEEESFK HHEEEHHHCCHHCCC	55.38	29967540
641	Ubiquitination	KLEEESFKMKNMFQV HCCHHCCCCCCCHHH	59.24	23000965
642	Ubiquitination	LEEESFKMKNMFQVI CCHHCCCCCCCHHHH	3.41	29967540
643	Ubiquitination	EEESFKMKNMFQVIQ CHHCCCCCCCHHHHC	45.94	23000965
674	Ubiquitination	DWIDILTKVSQCNQK HHHHHHHHHHHCCCC	36.12	29967540
703	Phosphorylation	WLCCRAPSDSAPGCS EEEEECCCCCCCCCC	43.12	28857561
714	Ubiquitination	PGCSPCTGGLPANIQ CCCCCCCCCCCCCEE	42.21	-
714	Ubiquitination	PGCSPCTGGLPANIQ CCCCCCCCCCCCCEE	42.21	29967540
746	Ubiquitination	LYMSKLEKMQEACGS HHHHHHHHHHHHHCC	57.21	2190698
749	Ubiquitination	SKLEKMQEACGSKSV HHHHHHHHHHCCCCC	42.29	29967540
755	Phosphorylation	QEACGSKSVYDGPEQ HHHHCCCCCCCCCCC	27.83	28796482
757	Phosphorylation	ACGSKSVYDGPEQEE HHCCCCCCCCCCCCE	23.44	28796482
765	Phosphorylation	DGPEQEEYSTFVIDD CCCCCCEEEEEEECC	16.56	28796482
766	Phosphorylation	GPEQEEYSTFVIDDP CCCCCEEEEEEECCH	20.74	28796482
767	Phosphorylation	PEQEEYSTFVIDDPQ CCCCEEEEEEECCHH	22.48	28796482
774	Ubiquitination	TFVIDDPQETYKTLK EEEECCHHHHHHHHH	63.76	29967540
776	Phosphorylation	VIDDPQETYKTLKQV EECCHHHHHHHHHHH	25.68	-
777	Phosphorylation	IDDPQETYKTLKQVI ECCHHHHHHHHHHHH	10.96	32645325
778	Ubiquitination	DDPQETYKTLKQVIA CCHHHHHHHHHHHHH	55.59	29967540
781	Ubiquitination	QETYKTLKQVIAGVG HHHHHHHHHHHHCHH	47.99	29967540
785	Ubiquitination	KTLKQVIAGVGALEQ HHHHHHHHCHHHHHH	14.04	29967540
797	Phosphorylation	LEQEHAQYKRDKFKK HHHHHHHHHHHHHCC	14.58	-
805	Phosphorylation	KRDKFKKTKYGSQEH HHHHHCCCCCCCCCC	30.04	23403867
806	Ubiquitination	RDKFKKTKYGSQEHP HHHHCCCCCCCCCCC	57.40	29967540
807	Phosphorylation	DKFKKTKYGSQEHPI HHHCCCCCCCCCCCC	27.52	23911959
809	Phosphorylation	FKKTKYGSQEHPIGD HCCCCCCCCCCCCCC	29.34	23401153
817	Ubiquitination	QEHPIGDKSFQNYIR CCCCCCCHHHHHHHH	48.21	29967540
818	Phosphorylation	EHPIGDKSFQNYIRQ CCCCCCHHHHHHHHH	36.48	26552605
822	Phosphorylation	GDKSFQNYIRQQSET CCHHHHHHHHHHCCC	6.16	29978859
827	Phosphorylation	QNYIRQQSETSTHSI HHHHHHHCCCCCCCC	34.21	25159151
829	Phosphorylation	YIRQQSETSTHSI-- HHHHHCCCCCCCC--	44.14	28450419
830	Phosphorylation	IRQQSETSTHSI--- HHHHCCCCCCCC---	21.77	28450419
831	Phosphorylation	RQQSETSTHSI---- HHHCCCCCCCC----	27.36	23401153
833	Phosphorylation	QSETSTHSI------ HCCCCCCCC------	30.04	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of RASA3_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of RASA3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of RASA3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049
SPTB1_HUMAN	SPTB	physical	22939629
RHG19_HUMAN	ARHGAP19	physical	22939629
GNAI2_HUMAN	GNAI2	physical	18952607
GNAI3_HUMAN	GNAI3	physical	18952607

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of RASA3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND THR-110, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Phosphoproteome of resting human platelets."; Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,Schuetz C., Walter U., Gambaryan S., Sickmann A.; J. Proteome Res. 7:526-534(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-809, AND MASSSPECTROMETRY.	18088087 [Abstract]
"Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-765, AND MASSSPECTROMETRY.	15592455 [Abstract]