dbPTM

SPTB1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SPTB1_HUMAN
UniProt AC	P11277
Protein Name	Spectrin beta chain, erythrocytic
Gene Name	SPTB
Organism	Homo sapiens (Human).
Sequence Length	2137
Subcellular Localization	Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
Protein Description	Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane..
Protein Sequence	MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLARVSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNALIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEKENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHLLEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRKAQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEAHGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGEDVGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLRQQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLAANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVESTKDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSLQGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRVKESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYTLAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNNEKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLENIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMEDQLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRRKKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQRGQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISDEIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAEERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNVNAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHRELPEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLDACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFSACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEPYLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAGEAPVSHHAATERTSPVSLWSRLSSSWESLQPEPSHPY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTSATEFEN ------CCCCCCCCC	38.07	28857561
3	Phosphorylation	-----MTSATEFENV -----CCCCCCCCCC	45.08	28857561
5	Phosphorylation	---MTSATEFENVGN ---CCCCCCCCCCCC	40.76	28857561
35	Phosphorylation	DELDNDNSSARLFER CCCCCCCHHHHHHHH	28.39	28857561
35 (in isoform 2)	Phosphorylation	-	28.39	27251275
36	Phosphorylation	ELDNDNSSARLFERS CCCCCCHHHHHHHHH	24.54	21082442
36 (in isoform 2)	Phosphorylation	-	24.54	-
57	Ubiquitination	DEREVVQKKTFTKWV CHHHHHHHHHHHHHH	42.02	23000965
58	Ubiquitination	EREVVQKKTFTKWVN HHHHHHHHHHHHHHH	31.60	23000965
62	Ubiquitination	VQKKTFTKWVNSHLA HHHHHHHHHHHHHHH	44.98	21890473
62 (in isoform 1)	Ubiquitination	-	44.98	21890473
62 (in isoform 2)	Ubiquitination	-	44.98	21890473
62 (in isoform 3)	Ubiquitination	-	44.98	21890473
66	Phosphorylation	TFTKWVNSHLARVSC HHHHHHHHHHHHHHH	15.40	28857561
76	Phosphorylation	ARVSCRITDLYKDLR HHHHHHHHHHHHHHH	10.70	-
80	Acetylation	CRITDLYKDLRDGRM HHHHHHHHHHHCCHH	58.55	19813157
90	Acetylation	RDGRMLIKLLEVLSG HCCHHHHHHHHHHCC	42.95	26051181
96	Phosphorylation	IKLLEVLSGEMLPKP HHHHHHHCCCCCCCC	37.57	18452278
96 (in isoform 2)	Phosphorylation	-	37.57	-
102	Ubiquitination	LSGEMLPKPTKGKMR HCCCCCCCCCCCCCC	62.45	-
104	Phosphorylation	GEMLPKPTKGKMRIH CCCCCCCCCCCCCCC	59.56	23186163
107	Ubiquitination	LPKPTKGKMRIHCLE CCCCCCCCCCCCHHC	26.93	-
118	Acetylation	HCLENVDKALQFLKE CHHCCHHHHHHHHHH	46.87	54421449
118	Ubiquitination	HCLENVDKALQFLKE CHHCCHHHHHHHHHH	46.87	30230243
124	Acetylation	DKALQFLKEQRVHLE HHHHHHHHHCCCCHH	53.76	26051181
124	Ubiquitination	DKALQFLKEQRVHLE HHHHHHHHHCCCCHH	53.76	33845483
133	Sulfoxidation	QRVHLENMGSHDIVD CCCCHHHCCCCCCCC	4.20	28183972
152	Phosphorylation	LVLGLIWTIILRFQI HHHHHHHHHHHHHHH	7.37	-
224	Acetylation	PDLIDFDKLKDSNAR CCCCCHHHHCCCCHH	58.38	25953088
224	Ubiquitination	PDLIDFDKLKDSNAR CCCCCHHHHCCCCHH	58.38	29967540
307	Phosphorylation	TEKMIEKYSGLASDL HHHHHHHHHCHHHHH	8.65	21951684
316	Phosphorylation	GLASDLLTWIEQTIT CHHHHHHHHHHHHHH	31.52	-
355	Acetylation	RTVEKPPKFQEKGNL CCCCCCCCCCCCCCE	69.11	7932607
379	Acetylation	RMRANNQKVYTPHDG HHHHCCCCCCCCCCC	39.05	7932619
390	Phosphorylation	PHDGKLVSDINRAWE CCCCCCHHHHHHHHH	43.12	23312004
398	Phosphorylation	DINRAWESLEEAEYR HHHHHHHHHHHHHHH	30.31	28258704
404	Phosphorylation	ESLEEAEYRRELALR HHHHHHHHHHHHHHH	23.24	28258704
419	Ubiquitination	NELIRQEKLEQLARR HHHHHHHHHHHHHHH	49.32	-
439	Phosphorylation	AMRETWLSENQRLVA HHHHHHHCHHHHHHH	26.51	25867546
461	Acetylation	LAAVEAAKKKHEAIE HHHHHHHHHHHHHCC	70.20	30705433
462	Glycation	AAVEAAKKKHEAIET HHHHHHHHHHHHCCC	55.42	-
474	Phosphorylation	IETDTAAYEERVRAL CCCCHHHHHHHHHHH	18.83	21951684
493	Phosphorylation	QELEKENYHDQKRIT HHHHHCCCCCHHCHH	14.09	21951684
497	Acetylation	KENYHDQKRITARKD HCCCCCHHCHHHCHH	52.40	30705439
519	Phosphorylation	YLQELLQSRRQRLET HHHHHHHHHHHHHHH	29.42	-
637	Acetylation	KAQLEQSKRLWKFFW HHHHHHHHHHHHHHH	51.72	30578653
660	Phosphorylation	IKEKEQIYSSLDYGK HHHHHHHHHHCCCCC	7.60	21951684
670	Phosphorylation	LDYGKDLTSVLILQR CCCCCCHHHHHHHHH	27.24	29083192
671	O-linked_Glycosylation	DYGKDLTSVLILQRK CCCCCHHHHHHHHHH	23.40	28411811
671	Phosphorylation	DYGKDLTSVLILQRK CCCCCHHHHHHHHHH	23.40	29083192
767	O-linked_Glycosylation	QDAHRLLSGEDVGQD HHHHHHHCCCCCCCC	44.73	12556401
979	Phosphorylation	DKTKVVESTKDLGRD CCHHHHHCCHHHCHH	29.19	-
998	Phosphorylation	IAIQRKLSGLERDVA HHHHHHHCCCHHHHH	53.03	23025827
998 (in isoform 2)	Phosphorylation	-	53.03	27251275
1018	Phosphorylation	VDALERESQQLMDSH HHHHHHHHHHHHHCC	29.46	24719451
1024	Phosphorylation	ESQQLMDSHPEQKED HHHHHHHCCHHHHHH	27.29	29759185
1226	Phosphorylation	NNRDKVLSPVDSGNK CCCCCCCCCCCCCCC	26.35	23025827
1226 (in isoform 2)	Phosphorylation	-	26.35	27251275
1242	Phosphorylation	VAEGNLYSDKIKEKV EEECCCCCHHHHHHH	35.28	-
1258	Acetylation	LIEDRHRKNNEKAQE HHHHHHHHCCHHHHH	58.98	88185
1262	Acetylation	RHRKNNEKAQEASVL HHHHCCHHHHHHHHH	58.45	88189
1296	Phosphorylation	WINDKLLTSQDVSYD HHCCCCCCCCCCCHH	34.48	23025827
1297	O-linked_Glycosylation	INDKLLTSQDVSYDE HCCCCCCCCCCCHHH	25.09	28411811
1297	Phosphorylation	INDKLLTSQDVSYDE HCCCCCCCCCCCHHH	25.09	-
1301	Phosphorylation	LLTSQDVSYDEARNL CCCCCCCCHHHHHHH	34.85	23025827
1302	Phosphorylation	LTSQDVSYDEARNLH CCCCCCCHHHHHHHH	20.04	23025827
1372	Phosphorylation	QATTKEKTQHLSAAR HHHCHHHHHHHHHHH	23.47	26437602
1376	Phosphorylation	KEKTQHLSAARSSDL HHHHHHHHHHHHCCC	20.12	26437602
1396	Phosphorylation	ADLNKWISAMEDQLR HHHHHHHHHHHHHHH	22.05	23025827
1444	Phosphorylation	ELFAQVPSMGEEGGD HHHHCCCCCCCCCCC	40.33	23025827
1455	Phosphorylation	EGGDADLSIEKRFLD CCCCCCCCHHHHHHH	29.28	23025827
1476	Phosphorylation	RRKKQLESSRAKLQI HHHHHHHHHHHHHHH	33.44	29083192
1477	Phosphorylation	RKKQLESSRAKLQIS HHHHHHHHHHHHHHC	27.21	29083192
1590	Phosphorylation	ANEAQQYYLDADEAE CHHHHHHHCCHHHHH	8.18	21951684
1652	O-linked_Glycosylation	SRAQGLLSAGHPEGE HHHHHHHHCCCCCHH	36.93	12556403
1672	Phosphorylation	QGQVDKHYAGLKDVA ECCCCHHHCCHHHHH	14.22	-
1796	Phosphorylation	RMQLLAASYDLHRYF HHHHHHHHHCHHHHH	17.36	21406692
1797	Phosphorylation	MQLLAASYDLHRYFY HHHHHHHHCHHHHHH	20.14	21406692
1829	Phosphorylation	EDVGLDASTAESFHR CCCCCCHHHHHHHHH	28.02	28060719
1830	Phosphorylation	DVGLDASTAESFHRV CCCCCHHHHHHHHHH	35.84	28060719
1833	Phosphorylation	LDASTAESFHRVHTA CCHHHHHHHHHHHHH	24.72	28060719
1928	O-linked_Glycosylation	SIIRQIETQERPRDV HHHHHHHCCCCCCCC	37.04	30379171
1936	O-linked_Glycosylation	QERPRDVSSVELLMK CCCCCCCCHHHHHHH	32.71	12556407
1960	Phosphorylation	ETRSKNFSACLELGE HHCCCCHHHHHHHHH	27.71	27251275
1989	Phosphorylation	EKLQQVMSRRKEMNE HHHHHHHHHHHHHHH	30.03	30108239
2031	Phosphorylation	WLIAQEPYLASGDFG HHHHCCCCCCCCCCC	17.72	28270605
2034	Phosphorylation	AQEPYLASGDFGHTV HCCCCCCCCCCCCCH	35.98	28270605
2040	Phosphorylation	ASGDFGHTVDSVEKL CCCCCCCCHHHHHHH	26.79	28270605
2043	Phosphorylation	DFGHTVDSVEKLIKR CCCCCHHHHHHHHHH	28.04	23025827
2043 (in isoform 2)	Phosphorylation	-	28.04	27251275
2057	Phosphorylation	RHEAFEKSTASWAER HHHHHHHHHHHHHHH	23.23	26437602
2058	Phosphorylation	HEAFEKSTASWAERF HHHHHHHHHHHHHHH	35.46	22673903
2060	Phosphorylation	AFEKSTASWAERFAA HHHHHHHHHHHHHHH	27.23	23025827
2060 (in isoform 2)	Phosphorylation	-	27.23	27251275
2072	Phosphorylation	FAALEKPTTLELKER HHHHCCCCCHHHHHH	55.89	28060719
2073	Phosphorylation	AALEKPTTLELKERQ HHHCCCCCHHHHHHH	27.11	28060719
2098	Phosphorylation	PQEEEGETAGEAPVS CCHHCCCCCCCCCCC	51.31	23186163
2105	Phosphorylation	TAGEAPVSHHAATER CCCCCCCCCCCCCCC	14.43	23186163
2110	Phosphorylation	PVSHHAATERTSPVS CCCCCCCCCCCCCHH	27.12	15065869
2113	Phosphorylation	HHAATERTSPVSLWS CCCCCCCCCCHHHHH	31.02	23025827
2113 (in isoform 2)	Phosphorylation	-	31.02	28270605
2114	Phosphorylation	HAATERTSPVSLWSR CCCCCCCCCHHHHHH	29.53	30266825
2114 (in isoform 2)	Phosphorylation	-	29.53	28270605
2117	Phosphorylation	TERTSPVSLWSRLSS CCCCCCHHHHHHHHC	27.75	30266825
2120	Phosphorylation	TSPVSLWSRLSSSWE CCCHHHHHHHHCCHH	28.74	23186163
2123	Phosphorylation	VSLWSRLSSSWESLQ HHHHHHHHCCHHHCC	22.63	30266825
2124	Phosphorylation	SLWSRLSSSWESLQP HHHHHHHCCHHHCCC	44.02	30266825
2125	Phosphorylation	LWSRLSSSWESLQPE HHHHHHCCHHHCCCC	31.33	30266825
2128	Phosphorylation	RLSSSWESLQPEPSH HHHCCHHHCCCCCCC	26.50	30266825
2134	Phosphorylation	ESLQPEPSHPY---- HHCCCCCCCCC----	35.12	29691806
2137	Phosphorylation	QPEPSHPY------- CCCCCCCC-------	26.26	29691806
2158 (in isoform 2)	Phosphorylation	-		24076635
2161 (in isoform 2)	Phosphorylation	-		24076635
2168 (in isoform 2)	Phosphorylation	-		28270605
2178 (in isoform 2)	Phosphorylation	-		-
2184 (in isoform 2)	Phosphorylation	-		-
2213 (in isoform 2)	Phosphorylation	-		-
2216 (in isoform 2)	Phosphorylation	-		-
2257	Ubiquitination	------------------------------------------------------------------------------------------------------------------------------- -------------------------------------------------------------------------------------------------------------------------------		32015554
2299 (in isoform 2)	Phosphorylation	-		23285258
2322 (in isoform 2)	Phosphorylation	-		26437602

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SPTB1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
2110	T	Phosphorylation	15065869
The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
2114	S	Phosphorylation	15065869
The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
2117	S	Phosphorylation	15065869
The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
2123	S	Phosphorylation	15065869
The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
2125	S	Phosphorylation	15065869
The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.
2128	S	Phosphorylation	15065869
The first phosphorylation event occurs on Ser-2114, followed by Ser-2125, Ser-2123, Ser-2128, Ser-2117, and Thr-2110.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SPTB1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
ANK1_HUMAN	ANK1	physical	2141335
41_HUMAN	EPB41	physical	7673158

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
182870	Elliptocytosis 3 (EL3)
182870	Spherocytosis 2 (SPH2)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SPTB1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"In vivo phosphorylation of human erythrocyte spectrin occurs in asequential manner."; Tang H.Y., Speicher D.W.; Biochemistry 43:4251-4262(2004). Cited for: PHOSPHORYLATION AT THR-2110; SER-2114; SER-2117; SER-2123; SER-2125AND SER-2128.	15065869 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2073, AND MASSSPECTROMETRY.	19413330 [Abstract]

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