RAD51_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RAD51_MOUSE
• UniProt AC: Q08297
• Protein Name: DNA repair protein RAD51 homolog 1
• Gene Name: Rad51
• Organism: Mus musculus (Mouse).
• Sequence Length: 339
• Subcellular Localization: Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Mitochondrion matrix . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage ind
• Protein Description: Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). [PubMed: 15834424 Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (By similarity]
• Protein Sequence: MAMQMQLEASADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGYHTVEAVAYAPKKELINIKGISEAKADKILTEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARGFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAMQMQLEA ------CCCCEEEEE	9.27	-
54	Phosphorylation	HTVEAVAYAPKKELI EEEEEEEECCHHHHE	19.48	-
309	Phosphorylation	LRKGRGETRICKIYD EECCCCCCEEEEEEC	28.32	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
54	Y	Phosphorylation	Kinase	ABL1	P00520	Uniprot
309	T	Phosphorylation	Kinase	CHEK1	O35280	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	Fbxo5	Q7TSG3	PMID:30554948

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
309	T	Phosphorylation	Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RAD51_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BRCA2_HUMAN	BRCA2	physical	9126738
BRCA2_MOUSE	Brca2	physical	11477095
BRCA1_MOUSE	Brca1	physical	11477095
BRCA2_MOUSE	Brca2	physical	9192668
R51A1_MOUSE	Rad51ap1	physical	9192668

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.