RAD18_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RAD18_MOUSE
• UniProt AC: Q9QXK2
• Protein Name: E3 ubiquitin-protein ligase RAD18
• Gene Name: Rad18
• Organism: Mus musculus (Mouse).
• Sequence Length: 509
• Subcellular Localization: Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with chromatin. Colocalizes with SLF1 in the nucleus and to centrosomes. Relocalizes with SLF1 to nuclear foci in response to DNA damage. Accumulates with the S
• Protein Description: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity..
• Protein Sequence: MEVLAEPRWPPGLAVMKTIDDLLRCGICFEYFNIAVIIPQCSHNYCSLCIRKFLSYKTQCPTCCVAVTEPDLRNNRLLDELVKSMNFARTHLLQFALESPPISPVSSTSKKVVVKVHNADAAQHPVKQANRLMDKFLIRETGDCVFELLGKENERKFSPQKELSTSAEIKETSLLGKPVLGLSDANGPVTPSTSTMKLDTKVSCPVCGVSIPENHINKHLDSCLSREEKKESLRSSAHKRKPLPKTVYNLLSDRDLKKKLKQYGLSVQGNKQQLIKRHQEFVHMYNAQCDALHPKSAAEIVQEIESMEKTRMRLEASKLNENVMVFTKNQTEKEIEEVHSEYRKKHQNAFQLLVDQAKKGYKKTGRVSQAAAMRTDEPAETLPSMRTDEPAETLPSMRTDEPAETLPLMRADEPAETLPSECIAQEDNVSFSDTVSVTNHFPQPQLDSPGPSEPERPDDSSSCTDILFSSDSDSCNRNDQNREVSPQQTRRTRASECVEIEPRNKRNKN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEVLAEPR -------CCCCCCCC	7.88	-
56	Phosphorylation	CIRKFLSYKTQCPTC HHHHHHHCCCCCCCE	21.87	-
90	Phosphorylation	KSMNFARTHLLQFAL HHCCHHHHHHHHHHH	17.28	25619855
99	Phosphorylation	LLQFALESPPISPVS HHHHHHCCCCCCCCC	36.50	27087446
103	Phosphorylation	ALESPPISPVSSTSK HHCCCCCCCCCCCCC	25.64	27087446
106	Phosphorylation	SPPISPVSSTSKKVV CCCCCCCCCCCCEEE	31.14	25619855
107	Phosphorylation	PPISPVSSTSKKVVV CCCCCCCCCCCEEEE	36.61	25619855
108	Phosphorylation	PISPVSSTSKKVVVK CCCCCCCCCCEEEEE	36.74	25619855
109	Phosphorylation	ISPVSSTSKKVVVKV CCCCCCCCCEEEEEE	32.21	25619855
127	Ubiquitination	DAAQHPVKQANRLMD CHHHCHHHHHHHHHH	48.41	-
135	Ubiquitination	QANRLMDKFLIRETG HHHHHHHHHHHHHHC	28.27	-
151	Ubiquitination	CVFELLGKENERKFS HHHHHCCCCCCCCCC	58.28	-
158	Phosphorylation	KENERKFSPQKELST CCCCCCCCCCCCCCC	29.47	25159016
161	Ubiquitination	ERKFSPQKELSTSAE CCCCCCCCCCCCCHH	65.10	-
164	Phosphorylation	FSPQKELSTSAEIKE CCCCCCCCCCHHHHH	22.77	28066266
165	Phosphorylation	SPQKELSTSAEIKET CCCCCCCCCHHHHHH	44.58	25159016
166	Phosphorylation	PQKELSTSAEIKETS CCCCCCCCHHHHHHH	22.11	28066266
177	Ubiquitination	KETSLLGKPVLGLSD HHHHCCCCCCCCCCC	31.74	-
190	Phosphorylation	SDANGPVTPSTSTMK CCCCCCCCCCCCCCC	18.04	26643407
192	Phosphorylation	ANGPVTPSTSTMKLD CCCCCCCCCCCCCCC	26.14	26643407
193	Phosphorylation	NGPVTPSTSTMKLDT CCCCCCCCCCCCCCC	29.44	26643407
194	Phosphorylation	GPVTPSTSTMKLDTK CCCCCCCCCCCCCCE	30.73	26643407
197	Ubiquitination	TPSTSTMKLDTKVSC CCCCCCCCCCCEEEC	43.21	-
318	Ubiquitination	RMRLEASKLNENVMV HHHHHHHHCCCCEEE	63.48	-
368	Phosphorylation	YKKTGRVSQAAAMRT HHHCCCHHHHHHHCC	16.85	-
485	Phosphorylation	NDQNREVSPQQTRRT CCCCCCCCHHHHHCC	16.55	26824392
489	Phosphorylation	REVSPQQTRRTRASE CCCCHHHHHCCCHHH	19.51	22345495
495	Phosphorylation	QTRRTRASECVEIEP HHHCCCHHHHEECCC	28.39	25266776

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RAD18_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RAD18_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RAD18_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
UBE2B_MOUSE	Ube2b	physical	17720710
UBE2A_MOUSE	Ube2a	physical	12509447
UBE2B_MOUSE	Ube2b	physical	12509447

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.