UBE2A_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: UBE2A_MOUSE
• UniProt AC: Q9Z255
• Protein Name: Ubiquitin-conjugating enzyme E2 A
• Gene Name: Ube2a
• Organism: Mus musculus (Mouse).
• Sequence Length: 152
• Protein Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA..
• Protein Sequence: MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSTPARRRL ------CCCHHHHHH	43.00	29895711
3	Phosphorylation	-----MSTPARRRLM -----CCCHHHHHHH	21.48	29895711
66	Ubiquitination	FTEEYPNKPPTVRFV ECCCCCCCCCEEEEE	48.12	-
66	Acetylation	FTEEYPNKPPTVRFV ECCCCCCCCCEEEEE	48.12	-
120	Phosphorylation	LDEPNPNSPANSQAA HCCCCCCCCCHHHHH	27.01	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
120	S	Phosphorylation	Kinase	CDK9	Q99J95	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
120	S	Phosphorylation	Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of UBE2A_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RAD18_MOUSE	Rad18	physical	12509447
H2B2B_MOUSE	Hist2h2bb	physical	11929833

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.