PREX1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PREX1_HUMAN
• UniProt AC: Q8TCU6
• Protein Name: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
• Gene Name: PREX1
• Organism: Homo sapiens (Human).
• Sequence Length: 1659
• Subcellular Localization: Cytoplasm, cytosol. Cell membrane. Mainly cytosolic. Some amount is apparently associated to the plasma membrane.
• Protein Description: Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils..
• Protein Sequence: MEAPSGSEPGGDGAGDCAHPDPRAPGAAAPSSGPGPCAAARESERQLRLRLCVLNEILGTERDYVGTLRFLQSAFLHRIRQNVADSVEKGLTEENVKVLFSNIEDILEVHKDFLAALEYCLHPEPQSQHELGNVFLKFKDKFCVYEEYCSNHEKALRLLVELNKIPTVRAFLLSCMLLGGRKTTDIPLEGYLLSPIQRICKYPLLLKELAKRTPGKHPDHPAVQSALQAMKTVCSNINETKRQMEKLEALEQLQSHIEGWEGSNLTDICTQLLLQGTLLKISAGNIQERAFFLFDNLLVYCKRKSRVTGSKKSTKRTKSINGSLYIFRGRINTEVMEVENVEDGTADYHSNGYTVTNGWKIHNTAKNKWFVCMAKTAEEKQKWLDAIIREREQRESLKLGMERDAYVMIAEKGEKLYHMMMNKKVNLIKDRRRKLSTVPKCFLGNEFVAWLLEIGEISKTEEGVNLGQALLENGIIHHVSDKHQFKNEQVMYRFRYDDGTYKARSELEDIMSKGVRLYCRLHSLYTPVIKDRDYHLKTYKSVLPGSKLVDWLLAQGDCQTREEAVALGVGLCNNGFMHHVLEKSEFRDESQYFRFHADEEMEGTSSKNKQLRNDFKLVENILAKRLLILPQEEDYGFDIEEKNKAVVVKSVQRGSLAEVAGLQVGRKIYSINEDLVFLRPFSEVESILNQSFCSRRPLRLLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVENCRRLMALSSAIVTMPHFEFRNICDTKLESIGQRIACYQEFAAQLKSRVSPPFKQAPLEPHPLCGLDFCPTNCHINLMEVSYPKTTPSVGRSFSIRFGRKPSLIGLDPEQGHLNPMSYTQHCITTMAAPSWKCLPAAEGDPQGQGLHDGSFGPASGTLGQEDRGLSFLLKQEDREIQDAYLQLFTKLDVALKEMKQYVTQINRLLSTITEPTSGGSCDASLAEEASSLPLVSEESEMDRSDHGGIKKVCFKVAEEDQEDSGHDTMSYRDSYSECNSNRDSVLSYTSVRSNSSYLGSDEMGSGDELPCDMRIPSDKQDKLHGCLEHLFNQVDSINALLKGPVMSRAFEETKHFPMNHSLQEFKQKEECTIRGRSLIQISIQEDPWNLPNSIKTLVDNIQRYVEDGKNQLLLALLKCTDTELQLRRDAIFCQALVAAVCTFSKQLLAALGYRYNNNGEYEESSRDASRKWLEQVAATGVLLHCQSLLSPATVKEERTMLEDIWVTLSELDNVTFSFKQLDENYVANTNVFYHIEGSRQALKVIFYLDSYHFSKLPSRLEGGASLRLHTALFTKVLENVEGLPSPGSQAAEDLQQDINAQSLEKVQQYYRKLRAFYLERSNLPTDASTTAVKIDQLIRPINALDELCRLMKSFVHPKPGAAGSVGAGLIPISSELCYRLGACQMVMCGTGMQRSTLSVSLEQAAILARSHGLLPKCIMQATDIMRKQGPRVEILAKNLRVKDQMPQGAPRLYRLCQPPVDGDL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Phosphorylation	APGAAAPSSGPGPCA CCCCCCCCCCCCCCH	43.11	28555341
32	Phosphorylation	PGAAAPSSGPGPCAA CCCCCCCCCCCCCHH	48.20	27251275
43	Phosphorylation	PCAAARESERQLRLR CCHHHHHCHHHHHHH	32.31	28857561
60	Phosphorylation	VLNEILGTERDYVGT HHHHHHCCCCCHHHH	25.13	24719451
145	Phosphorylation	FKDKFCVYEEYCSNH CCCCEEEHHHHCCCH	12.29	27642862
174	Phosphorylation	TVRAFLLSCMLLGGR HHHHHHHHHHHHCCC	10.30	-
182	Ubiquitination	CMLLGGRKTTDIPLE HHHHCCCCCCCCCCC	60.08	-
201	Ubiquitination	SPIQRICKYPLLLKE HHHHHHCHHHHHHHH	47.75	-
216	Ubiquitination	LAKRTPGKHPDHPAV HHHCCCCCCCCCHHH	53.84	29967540
224	Ubiquitination	HPDHPAVQSALQAMK CCCCHHHHHHHHHHH	25.70	21890473
235	Phosphorylation	QAMKTVCSNINETKR HHHHHHHHCCHHHHH	36.09	27251275
241	Acetylation	CSNINETKRQMEKLE HHCCHHHHHHHHHHH	34.16	26051181
241	Ubiquitination	CSNINETKRQMEKLE HHCCHHHHHHHHHHH	34.16	30230243
308	Phosphorylation	CKRKSRVTGSKKSTK HHHHHCCCCCCCCCC	34.33	-
310	Phosphorylation	RKSRVTGSKKSTKRT HHHCCCCCCCCCCCE	27.78	-
313	Phosphorylation	RVTGSKKSTKRTKSI CCCCCCCCCCCEECC	42.78	26670566
314	Phosphorylation	VTGSKKSTKRTKSIN CCCCCCCCCCEECCC	33.16	26670566
317	Phosphorylation	SKKSTKRTKSINGSL CCCCCCCEECCCCEE	30.60	25599653
319	Phosphorylation	KSTKRTKSINGSLYI CCCCCEECCCCEEEE	22.22	25159151
323	Phosphorylation	RTKSINGSLYIFRGR CEECCCCEEEEEECC	17.32	23403867
325	Phosphorylation	KSINGSLYIFRGRIN ECCCCEEEEEECCCC	10.34	25599653
333	Phosphorylation	IFRGRINTEVMEVEN EEECCCCCEEEEEEE	27.82	-
406	Phosphorylation	LGMERDAYVMIAEKG CCCCCCEEEEEEECC	8.54	17322306
417	Phosphorylation	AEKGEKLYHMMMNKK EECCHHHHHHHHHCC	9.95	29083192
436	Phosphorylation	KDRRRKLSTVPKCFL HHHHHHHCCCCCCCC	30.32	22817900
437	Phosphorylation	DRRRKLSTVPKCFLG HHHHHHCCCCCCCCC	51.79	23312004
482	Ubiquitination	IIHHVSDKHQFKNEQ CEEECCCCHHCCCCE	31.62	29967540
492	Phosphorylation	FKNEQVMYRFRYDDG CCCCEEEEEEECCCC	14.03	29978859
496	Phosphorylation	QVMYRFRYDDGTYKA EEEEEEECCCCCEEE	18.70	29978859
500	Phosphorylation	RFRYDDGTYKARSEL EEECCCCCEEEHHHH	28.92	29978859
501	Phosphorylation	FRYDDGTYKARSELE EECCCCCEEEHHHHH	14.26	29978859
505	Phosphorylation	DGTYKARSELEDIMS CCCEEEHHHHHHHHH	51.93	29449344
512	Phosphorylation	SELEDIMSKGVRLYC HHHHHHHHHHHHHHH	26.96	29449344
523	Phosphorylation	RLYCRLHSLYTPVIK HHHHHHHHCCCCEEC	27.88	28857561
525	Phosphorylation	YCRLHSLYTPVIKDR HHHHHHCCCCEECCC	16.41	27642862
526	Phosphorylation	CRLHSLYTPVIKDRD HHHHHCCCCEECCCC	19.17	23312004
537	Ubiquitination	KDRDYHLKTYKSVLP CCCCCCCCCCHHHCC	36.17	29967540
590	Phosphorylation	KSEFRDESQYFRFHA CCCCCCHHHCEEEEC	34.17	27251275
592	Phosphorylation	EFRDESQYFRFHADE CCCCHHHCEEEECCH	13.15	27642862
605	Phosphorylation	DEEMEGTSSKNKQLR CHHHCCCCHHCHHHH	50.61	21042280
624	Ubiquitination	LVENILAKRLLILPQ HHHHHHHHHCCCCCC	39.88	22817900
624 (in isoform 1)	Ubiquitination	-	39.88	21890473
624 (in isoform 3)	Ubiquitination	-	39.88	21890473
642	Acetylation	YGFDIEEKNKAVVVK CCCCHHHCCEEEEEE	51.46	7467723
649	Ubiquitination	KNKAVVVKSVQRGSL CCEEEEEEEECCCCH	33.04	-
682	Phosphorylation	LVFLRPFSEVESILN EEEEECHHHHHHHHC	43.33	28270605
686	Phosphorylation	RPFSEVESILNQSFC ECHHHHHHHHCCCCC	37.24	28270605
691	Phosphorylation	VESILNQSFCSRRPL HHHHHCCCCCCCCCH	27.44	28270605
694	Phosphorylation	ILNQSFCSRRPLRLL HHCCCCCCCCCHHHH	29.34	28270605
704	Phosphorylation	PLRLLVATKAKEIIK CHHHHEEECCHHHCC	24.85	-
707	Ubiquitination	LLVATKAKEIIKIPD HHEEECCHHHCCCCC	51.51	-
805	Phosphorylation	ARASQEASTEDPSGE HHHHHHCCCCCCCCH	31.41	22242915
806	Phosphorylation	RASQEASTEDPSGEQ HHHHHCCCCCCCCHH	52.00	21712546
834	Phosphorylation	LSLGPRLSLCEDSPM HHCCCCEECCCCCCC	32.05	26074081
926	Ubiquitination	FRNICDTKLESIGQR HHCCCCCCHHHHHHH	36.69	-
945	Ubiquitination	QEFAAQLKSRVSPPF HHHHHHHHHCCCCCC	24.92	-
980	Phosphorylation	HINLMEVSYPKTTPS EEEEEEECCCCCCCC	23.55	24719451
985	O-linked_Glycosylation	EVSYPKTTPSVGRSF EECCCCCCCCCCCCE	21.49	29351928
987	O-linked_Glycosylation	SYPKTTPSVGRSFSI CCCCCCCCCCCCEEE	34.80	29351928
991	Phosphorylation	TTPSVGRSFSIRFGR CCCCCCCCEEEEECC	19.88	24719451
993	Phosphorylation	PSVGRSFSIRFGRKP CCCCCCEEEEECCCC	17.69	24719451
999	Sumoylation	FSIRFGRKPSLIGLD EEEEECCCCHHEEEC	39.06	-
1001	Phosphorylation	IRFGRKPSLIGLDPE EEECCCCHHEEECCC	35.34	28450419
1016	Phosphorylation	QGHLNPMSYTQHCIT CCCCCCCCCHHHHHH	26.70	28450419
1017	Phosphorylation	GHLNPMSYTQHCITT CCCCCCCCHHHHHHH	12.27	28450419
1018	Phosphorylation	HLNPMSYTQHCITTM CCCCCCCHHHHHHHC	12.44	28450419
1049	Phosphorylation	GQGLHDGSFGPASGT CCCCCCCCCCCCCCC	32.84	28450419
1054	Phosphorylation	DGSFGPASGTLGQED CCCCCCCCCCCCCHH	35.13	28450419
1056	Phosphorylation	SFGPASGTLGQEDRG CCCCCCCCCCCHHHC	25.81	28450419
1065	Phosphorylation	GQEDRGLSFLLKQED CCHHHCHHHHEEHHC	19.37	27251275
1069	Ubiquitination	RGLSFLLKQEDREIQ HCHHHHEEHHCHHHH	54.51	29967540
1111	Phosphorylation	LSTITEPTSGGSCDA HHCCCCCCCCCCCCH	33.50	28348404
1112	Phosphorylation	STITEPTSGGSCDAS HCCCCCCCCCCCCHH	52.45	28348404
1115	Phosphorylation	TEPTSGGSCDASLAE CCCCCCCCCCHHHHH	16.61	28348404
1119	Phosphorylation	SGGSCDASLAEEASS CCCCCCHHHHHHHHC	18.18	28348404
1125	Phosphorylation	ASLAEEASSLPLVSE HHHHHHHHCCCCCCC	35.37	22242915
1126	Phosphorylation	SLAEEASSLPLVSEE HHHHHHHCCCCCCCC	40.84	28348404
1131	Phosphorylation	ASSLPLVSEESEMDR HHCCCCCCCCCCCCC	43.24	28348404
1134	Phosphorylation	LPLVSEESEMDRSDH CCCCCCCCCCCCCCC	33.88	28348404
1139	Phosphorylation	EESEMDRSDHGGIKK CCCCCCCCCCCCHHH	29.69	28348404
1159	Phosphorylation	AEEDQEDSGHDTMSY ECCCCCCCCCCCCCH	36.96	28450419
1163	Phosphorylation	QEDSGHDTMSYRDSY CCCCCCCCCCHHHCC	11.44	22210691
1165	Phosphorylation	DSGHDTMSYRDSYSE CCCCCCCCHHHCCHH	21.07	28450419
1166	Phosphorylation	SGHDTMSYRDSYSEC CCCCCCCHHHCCHHH	13.97	28450419
1169	Phosphorylation	DTMSYRDSYSECNSN CCCCHHHCCHHHCCC	22.89	27251275
1179	Phosphorylation	ECNSNRDSVLSYTSV HHCCCCCCCCEEEEC	23.01	28348404
1182	Phosphorylation	SNRDSVLSYTSVRSN CCCCCCCEEEECCCC	24.98	21815630
1183	Phosphorylation	NRDSVLSYTSVRSNS CCCCCCEEEECCCCC	10.00	27642862
1184	Phosphorylation	RDSVLSYTSVRSNSS CCCCCEEEECCCCCC	19.85	30108239
1185	Phosphorylation	DSVLSYTSVRSNSSY CCCCEEEECCCCCCC	13.80	30108239
1188	Phosphorylation	LSYTSVRSNSSYLGS CEEEECCCCCCCCCC	38.62	23403867
1190	Phosphorylation	YTSVRSNSSYLGSDE EEECCCCCCCCCCCC	22.93	23403867
1191	Phosphorylation	TSVRSNSSYLGSDEM EECCCCCCCCCCCCC	28.73	25159151
1192	Phosphorylation	SVRSNSSYLGSDEMG ECCCCCCCCCCCCCC	18.20	23403867
1195	Phosphorylation	SNSSYLGSDEMGSGD CCCCCCCCCCCCCCC	28.00	23403867
1200	Phosphorylation	LGSDEMGSGDELPCD CCCCCCCCCCCCCCC	41.58	23401153
1209	Methylation	DELPCDMRIPSDKQD CCCCCCCCCCCCHHC	25.15	115387791
1249	Ubiquitination	SRAFEETKHFPMNHS HHHHHHHCCCCCCCC	46.67	29967540
1256	Phosphorylation	KHFPMNHSLQEFKQK CCCCCCCCHHHHHCH	27.09	26657352
1272	Phosphorylation	ECTIRGRSLIQISIQ CCEECCCEEEEEEEC	32.42	26657352
1277	Phosphorylation	GRSLIQISIQEDPWN CCEEEEEEECCCCCC	11.22	28450419
1337	Phosphorylation	ALVAAVCTFSKQLLA HHHHHHHHHHHHHHH	25.09	22210691
1356	Phosphorylation	RYNNNGEYEESSRDA CCCCCCCCCCCCHHH	26.79	22210691
1359	Phosphorylation	NNGEYEESSRDASRK CCCCCCCCCHHHCHH	20.80	22210691
1382	O-linked_Glycosylation	GVLLHCQSLLSPATV HHHHHHHHHCCCCCH	36.35	29351928
1412	Phosphorylation	ELDNVTFSFKQLDEN HHCCCEEEEEECCCC	23.36	24719451
1442	Phosphorylation	QALKVIFYLDSYHFS HHHHHHEEECHHCHH	9.85	22817900
1480	Phosphorylation	ENVEGLPSPGSQAAE HHCCCCCCCCHHHHH	47.11	32142685
1483	Phosphorylation	EGLPSPGSQAAEDLQ CCCCCCCHHHHHHHH	21.74	28348404
1500	Ubiquitination	INAQSLEKVQQYYRK HHHHHHHHHHHHHHH	51.11	29967540
1525	Phosphorylation	LPTDASTTAVKIDQL CCCCCCCCEEEHHHH	27.27	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
313	S	Phosphorylation	Kinase	PRKCD	Q05655	GPS
436	S	Phosphorylation	Kinase	PRKACA	P17612	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PREX1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PREX1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MTOR_HUMAN	MTOR	physical	17565979
RPTOR_HUMAN	RPTOR	physical	21339740
RICTR_HUMAN	RICTOR	physical	21339740
AKT1_HUMAN	AKT1	physical	21339740

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.";
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.;
Anal. Sci. 24:161-166(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-436, AND MASSSPECTROMETRY.
PubMed: 18187866