PRAF3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PRAF3_HUMAN
• UniProt AC: O75915
• Protein Name: PRA1 family protein 3
• Gene Name: ARL6IP5
• Organism: Homo sapiens (Human).
• Sequence Length: 188
• Subcellular Localization: Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cytoplasm . Cytoplasm, cytoskeleton . Also exists as a soluble form in the cytoplasm. Associated with microtubules.
• Protein Description: Regulates intracellular concentrations of taurine and glutamate. Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner. May be involved in membrane traffic..
• Protein Sequence: MDVNIAPLRAWDDFFPGSDRFARPDFRDISKWNNRVVSNLLYYQTNYLVVAAMMISIVGFLSPFNMILGGIVVVLVFTGFVWAAHNKDVLRRMKKRYPTTFVMVVMLASYFLISMFGGVMVFVFGITFPLLLMFIHASLRLRNLKNKLENKMEGIGLKRTPMGIVLDALEQQEEGINRLTDYISKVKE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MDVNIAPL -------CCCCCCCC	13.49	22223895
18	Phosphorylation	WDDFFPGSDRFARPD CCCCCCCCCCCCCCC	26.33	21712546
31	Ubiquitination	PDFRDISKWNNRVVS CCCCCHHHHCHHHHH	55.03	21890473
31	Acetylation	PDFRDISKWNNRVVS CCCCCHHHHCHHHHH	55.03	25953088
31	Malonylation	PDFRDISKWNNRVVS CCCCCHHHHCHHHHH	55.03	26320211
31	Ubiquitination	PDFRDISKWNNRVVS CCCCCHHHHCHHHHH	55.03	21890473
151	Acetylation	LKNKLENKMEGIGLK HHHHHHHHCCCCCCC	29.02	27452117
151	Ubiquitination	LKNKLENKMEGIGLK HHHHHHHHCCCCCCC	29.02	21890473
152	Sulfoxidation	KNKLENKMEGIGLKR HHHHHHHCCCCCCCC	9.58	21406390
158	2-Hydroxyisobutyrylation	KMEGIGLKRTPMGIV HCCCCCCCCCCCCHH	49.35	-
158	Ubiquitination	KMEGIGLKRTPMGIV HCCCCCCCCCCCCHH	49.35	21890473
180	Phosphorylation	EEGINRLTDYISKVK HHHHHHHHHHHHHHC	24.42	26552605
182	Phosphorylation	GINRLTDYISKVKE- HHHHHHHHHHHHCC-	11.23	29978859
184	Phosphorylation	NRLTDYISKVKE--- HHHHHHHHHHCC---	25.76	30108239
185	Ubiquitination	RLTDYISKVKE---- HHHHHHHHHCC----	48.01	21890473
185	Malonylation	RLTDYISKVKE---- HHHHHHHHHCC----	48.01	26320211
185	Ubiquitination	RLTDYISKVKE---- HHHHHHHHHCC----	48.01	21890473

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PRAF3_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PRAF3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PRAF3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
XRCC1_HUMAN	XRCC1	physical	19208635
RL5_HUMAN	RPL5	physical	22939629
USP9X_HUMAN	USP9X	physical	22939629
RN185_HUMAN	RNF185	physical	25416956

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Exploring proteomes and analyzing protein processing by massspectrometric identification of sorted N-terminal peptides.";
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A.,Thomas G.R., Vandekerckhove J.;
Nat. Biotechnol. 21:566-569(2003).
Cited for: PROTEIN SEQUENCE OF 1-9, AND ACETYLATION AT MET-1.
PubMed: 12665801