PLPL2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PLPL2_MOUSE
• UniProt AC: Q8BJ56
• Protein Name: Patatin-like phospholipase domain-containing protein 2
• Gene Name: Pnpla2
• Organism: Mus musculus (Mouse).
• Sequence Length: 486
• Subcellular Localization: Lipid droplet. Cell membrane; Single-pass type II membrane protein.
• Protein Description: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. [PubMed: 15550674 Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.]
• Protein Sequence: MFPRETKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEAGANIIEVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGENVIISHFSSKDELIQANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPPVVPQEEDAEEAAVVEERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAMMVPYTLPLESAVSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGDHLPSRLSEQVELRRAQSLPSVPLSCATYSEALPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPATPQDPPGLPPC

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
39	N-linked_Glycosylation	HAPFLVANATHIYGA HCCEEEEECCEEECC	37.58	-
87	Phosphorylation	FLGPLHPSFNLVKTI HCCCCCCCHHHHHHH	18.93	-
101	Phosphorylation	IRGCLLKTLPADCHE HHHHHHHCCCCCHHH	37.63	-
210	Phosphorylation	HELRVTNTSIQFNLR EEEEECCCEEEEEHH	20.17	-
224	Ubiquitination	RNLYRLSKALFPPEP HHHHHHHHHHCCCCH	54.42	22790023
295	Ubiquitination	DQLQPYRKDRILEHL CCCCCHHHHHHHHHH	45.58	22790023
372	Phosphorylation	IRWMKEQTGSICQYL HHHHHHHHHCHHHHH	33.71	-
374	Phosphorylation	WMKEQTGSICQYLVM HHHHHHHCHHHHHHH	24.47	22733971
378	Phosphorylation	QTGSICQYLVMRAKR HHHCHHHHHHHHHHH	9.24	-
393	Phosphorylation	KLGDHLPSRLSEQVE HHCCCCCHHHHHHHH	52.81	-
396	Phosphorylation	DHLPSRLSEQVELRR CCCCHHHHHHHHHHH	25.52	22733971
406	Phosphorylation	VELRRAQSLPSVPLS HHHHHHHCCCCCCCC	41.06	27087446
409	Phosphorylation	RRAQSLPSVPLSCAT HHHHCCCCCCCCCCC	41.48	21082442
413	Phosphorylation	SLPSVPLSCATYSEA CCCCCCCCCCCHHHH	8.81	25159016
416	Phosphorylation	SVPLSCATYSEALPN CCCCCCCCHHHHCCH	31.72	25159016
417	Phosphorylation	VPLSCATYSEALPNW CCCCCCCHHHHCCHH	5.98	25159016
418	Phosphorylation	PLSCATYSEALPNWV CCCCCCHHHHCCHHH	16.43	25159016
430	Phosphorylation	NWVRNNLSLGDALAK HHHHCCCCHHHHHHH	32.22	27742792
468	Phosphorylation	LRMRAPASPTAADPA HHCCCCCCCCCCCCC	23.02	25619855
470	Phosphorylation	MRAPASPTAADPATP CCCCCCCCCCCCCCC	31.79	25619855
476	Phosphorylation	PTAADPATPQDPPGL CCCCCCCCCCCCCCC	27.20	25619855

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
396	S	Phosphorylation	Kinase	PKA	-	Uniprot
406	S	Phosphorylation	Kinase	AMPKA1	Q13131	PSP
406	S	Phosphorylation	Kinase	PRKAA1	Q5EG47	GPS
406	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
406	S	Phosphorylation	Phosphorylation at Ser-406 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity.	21183079

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PLPL2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
PLPL2_MOUSE	Pnpla2	physical	19692632
PLIN5_MOUSE	Plin5	physical	21393244
G0S2_MOUSE	G0s2	physical	20197052
ABHD5_MOUSE	Abhd5	physical	24440819

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.