PIWL1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: PIWL1_HUMAN
• UniProt AC: Q96J94
• Protein Name: Piwi-like protein 1
• Gene Name: PIWIL1
• Organism: Homo sapiens (Human).
• Sequence Length: 861
• Subcellular Localization: Cytoplasm . Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Also present in chromatoid body.
• Protein Description: Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1.; Isoform 3: May be a negative developmental regulator. [PubMed: 12037681]
• Protein Sequence: MTGRARARARGRARGQETAQLVGSTASQQPGYIQPRPQPPPAEGELFGRGRQRGTAGGTAKSQGLQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVLRSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKNDNVQRELRDWGLSFDSNLLSFSGRILQTEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMRKAIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASINEGMTRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
14	Symmetric dimethylarginine	ARARGRARGQETAQL HHHHHHHCCHHHHHH	46.06	-
14	Methylation	ARARGRARGQETAQL HHHHHHHCCHHHHHH	46.06	-
18	Phosphorylation	GRARGQETAQLVGST HHHCCHHHHHHHCCC	16.30	29978859
24	Phosphorylation	ETAQLVGSTASQQPG HHHHHHCCCCCCCCC	17.20	29978859
25	Phosphorylation	TAQLVGSTASQQPGY HHHHHCCCCCCCCCC	25.13	29978859
27	Phosphorylation	QLVGSTASQQPGYIQ HHHCCCCCCCCCCCC	29.45	29978859
32	Phosphorylation	TASQQPGYIQPRPQP CCCCCCCCCCCCCCC	11.76	29978859
49	Methylation	AEGELFGRGRQRGTA CCCCCCCCCCCCCCC	29.77	-
53	Methylation	LFGRGRQRGTAGGTA CCCCCCCCCCCCCCC	42.97	-
55	Phosphorylation	GRGRQRGTAGGTAKS CCCCCCCCCCCCCHH	25.07	22210691
107	Phosphorylation	VKESKTGSSGIIVRL CHHCCCCCCCEEEEE	30.53	-
122	Phosphorylation	STNHFRLTSRPQWAL ECCCEEECCCCCEEE	20.59	-
123	Phosphorylation	TNHFRLTSRPQWALY CCCEEECCCCCEEEE	46.70	-
183	Phosphorylation	QKVTEVFSKTRNGED HHHHHHHHHCCCCCE	37.99	24505115
185	Phosphorylation	VTEVFSKTRNGEDVR HHHHHHHCCCCCEEE	28.33	24505115
338	Phosphorylation	TFKKADGSEVSFLEY CCCCCCCCCCHHHHH	35.06	24719451
370	Methylation	VSQPKRRRGPGGTLP ECCCCCCCCCCCCCC	60.74	-
388	Phosphorylation	MLIPELCYLTGLTDK HHHHHHHHHHCCHHH	21.66	28634298
390	Phosphorylation	IPELCYLTGLTDKMR HHHHHHHHCCHHHHH	12.04	28634298
393	Phosphorylation	LCYLTGLTDKMRNDF HHHHHCCHHHHHCCC	34.61	28634298
410	Phosphorylation	MKDLAVHTRLTPEQR HHHHHHHHCCCHHHH	22.30	30576142
413	Phosphorylation	LAVHTRLTPEQRQRE HHHHHCCCHHHHHHH	22.84	30576142
489	Phosphorylation	TRGAPLISVKPLDNW CCCCCEEEEEECCCE	31.60	24719451
517 (in isoform 3)	Ubiquitination	-	48.65	21906983
519	Phosphorylation	IQNLFKVTPAMGMQM HHHHHHCCHHCCCCC	13.11	24114839
550	Phosphorylation	RVLQQKVTADTQIVV HHHHHHCCCCCEEEE	26.33	29507054
561	Phosphorylation	QIVVCLLSSNRKDKY EEEEEEECCCCCCHH	16.78	29507054
562	Phosphorylation	IVVCLLSSNRKDKYD EEEEEECCCCCCHHH	40.39	29507054
564	Methylation	VCLLSSNRKDKYDAI EEEECCCCCCHHHHH	50.96	115388099
603	Ubiquitination	TVMAIATKIALQMNC HHHHHHHHHHHHHCC	19.57	2190698
603 (in isoform 2)	Ubiquitination	-	19.57	21906983
603 (in isoform 1)	Ubiquitination	-	19.57	21906983
643	Phosphorylation	DMTAGRRSIAGFVAS CCCCCCCCHHHHHHH	18.54	-
650	Phosphorylation	SIAGFVASINEGMTR CHHHHHHHHCHHHHH	22.20	-
736	Phosphorylation	RGYNPRLTVIVVKKR CCCCCCEEEEEEECC	14.96	-
781	Phosphorylation	WYDFFIVSQAVRSGS HEEEEEEEHHHHCCC	13.96	26074081
786	Phosphorylation	IVSQAVRSGSVSPTH EEEHHHHCCCCCCCE	28.90	26074081
788	Phosphorylation	SQAVRSGSVSPTHYN EHHHHCCCCCCCEEE	22.22	26074081
790	Phosphorylation	AVRSGSVSPTHYNVI HHHCCCCCCCEEEEE	26.27	26074081
792	Phosphorylation	RSGSVSPTHYNVIYD HCCCCCCCEEEEEEC	29.50	26074081
794	Phosphorylation	GSVSPTHYNVIYDNS CCCCCCEEEEEECCC	17.38	26074081
798	Phosphorylation	PTHYNVIYDNSGLKP CCEEEEEECCCCCCH	12.56	26074081
801	Phosphorylation	YNVIYDNSGLKPDHI EEEEECCCCCCHHHH	42.47	26074081
860	Phosphorylation	SLSNRLYYL------ CCCCCEECC------	15.55	18083107

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of PIWL1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of PIWL1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of PIWL1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DICER_HUMAN	DICER1	physical	14749716
STMN1_HUMAN	STMN1	physical	26317901
TBA1B_HUMAN	TUBA1B	physical	26317901
TBB3_HUMAN	TUBB3	physical	26317901

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.