dbPTM

PIP21_ARATH - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PIP21_ARATH
UniProt AC	P43286
Protein Name	Aquaporin PIP2-1
Gene Name	PIP2-1
Organism	Arabidopsis thaliana (Mouse-ear cress).
Sequence Length	287
Subcellular Localization	Cell membrane Multi-pass membrane protein . A fuzzy intracellular localization is induced by salt (NaCl) treatment.
Protein Description	Water channel required to facilitate the transport of water across cell membrane. Probably involved in root water uptake. Its function is impaired by Hg(2+)..
Protein Sequence	MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVIGYKIQSDTDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MAKDVEAV -------CCCCCCCC	10.63	-
2	Acetylation	------MAKDVEAVP ------CCCCCCCCC	19.43	-
3	"N6,N6-dimethyllysine"	-----MAKDVEAVPG -----CCCCCCCCCC	62.09	-
3	Methylation	-----MAKDVEAVPG -----CCCCCCCCCC	62.09	16839310
277	Phosphorylation	LRASGSKSLGSFRSA HHHCCCCCCCCHHHC	39.39	23776212
280	Phosphorylation	SGSKSLGSFRSAANV CCCCCCCCHHHCCCC	23.84	30291188
283	Phosphorylation	KSLGSFRSAANV--- CCCCCHHHCCCC---	29.71	30291188

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	RMA1	O64425	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
280	S	Phosphorylation	18234664
The phosphorylation at Ser-280 and Ser-283 is altered by salt (NaCl) and hydrogen peroxide H(2)O(2) treatments.
283	S	Phosphorylation	18234664
Phosphorylation of Ser-283 is required for plasma membrane targeting.
283	S	Phosphorylation	18234664
The phosphorylation at Ser-280 and Ser-283 is altered by salt (NaCl) and hydrogen peroxide H(2)O(2) treatments.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PIP21_ARATH !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
YKT61_ARATH	YKT61	physical	22737156
PTR2_ARATH	PTR2	physical	22737156
PIP21_ARATH	PIP2A	physical	21423366
CHX9_ARATH	CHX9	physical	21423366

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PIP21_ARATH

Related Literatures of Post-Translational Modification

Methylation
Reference	PubMed
"Methylation of aquaporins in plant plasma membrane."; Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C.; Biochem. J. 400:189-197(2006). Cited for: METHYLATION AT LYS-3, MUTAGENESIS OF LYS-3 AND GLU-6, AND MASSSPECTROMETRY.	16839310 [Abstract]
Phosphorylation
Reference	PubMed
"Multiple phosphorylations in the C-terminal tail of plant plasmamembrane aquaporins: role in subcellular trafficking of AtPIP2;1 inresponse to salt stress."; Prak S., Hem S., Boudet J., Viennois G., Sommerer N., Rossignol M.,Maurel C., Santoni V.; Mol. Cell. Proteomics 7:1019-1030(2008). Cited for: PHOSPHORYLATION AT SER-280 AND SER-283, SUBCELLULAR LOCATION,MUTAGENESIS OF SER-280 AND SER-283, AND IDENTIFICATION BY MASSSPECTROMETRY.	18234664 [Abstract]
"Phosphoproteomics of the Arabidopsis plasma membrane and a newphosphorylation site database."; Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.; Plant Cell 16:2394-2405(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280 AND SER-283, ANDMASS SPECTROMETRY.	15308754 [Abstract]