dbPTM

PARG_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PARG_HUMAN
UniProt AC	Q86W56
Protein Name	Poly(ADP-ribose) glycohydrolase
Gene Name	PARG
Organism	Homo sapiens (Human).
Sequence Length	976
Subcellular Localization	Isoform 1: Nucleus . Colocalizes with PCNA at replication foci. Relocalizes to the cytoplasm in response to DNA damage. Isoform 2: Cytoplasm . Translocates to the nucleus in response to DNA damage. Isoform 3: Cytoplasm. Isoform 4: Cytoplasm.
Protein Description	Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. [PubMed: 23102699 PARG acts both as an endo- and exoglycosidase, releasing PAR of different length as well as ADP-ribose monomers]
Protein Sequence	MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSATSLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTEKSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLRAGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKYNVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQIASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQSLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRLFTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEKMRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRDIYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1 (in isoform 2)	Acetylation	-	7.57	22814378
12	Acetylation	PGCEPCTKRPRWGAA CCCCCCCCCCCCCCC	68.10	25953088
20	Phosphorylation	RPRWGAATTSPAASD CCCCCCCCCCCCHHH	27.54	27732954
21	Phosphorylation	PRWGAATTSPAASDA CCCCCCCCCCCHHHH	28.17	21815630
22	Phosphorylation	RWGAATTSPAASDAR CCCCCCCCCCHHHHH	14.26	16964243
26	Phosphorylation	ATTSPAASDARSFPS CCCCCCHHHHHCCCC	33.63	27732954
29	Phosphorylation	SPAASDARSFPSRQR CCCHHHHHCCCCHHC	43.77	32142685
33	Ubiquitination	SDARSFPSRQRRVLD HHHHCCCCHHCCCCC	38.75	29967540
53	Phosphorylation	VQFRVPPSSPACVPG EEEECCCCCCCCCCC	42.77	26552605
54	Phosphorylation	QFRVPPSSPACVPGR EEECCCCCCCCCCCC	23.45	25159151
55	Phosphorylation	FRVPPSSPACVPGRA EECCCCCCCCCCCCC	33.73	32142685
65	Ubiquitination	VPGRAGQHRGSATSL CCCCCCCCCCCCEEE	34.84	22817900
66	Methylation	PGRAGQHRGSATSLV CCCCCCCCCCCEEEE	32.64	115486421
68	Phosphorylation	RAGQHRGSATSLVFK CCCCCCCCCEEEEEE	28.47	23917254
69	Ubiquitination	AGQHRGSATSLVFKQ CCCCCCCCEEEEEEC	12.20	22817900
70	Ubiquitination	GQHRGSATSLVFKQK CCCCCCCEEEEEECC	25.04	21890473
71	Phosphorylation	QHRGSATSLVFKQKT CCCCCCEEEEEECCC	23.42	28555341
75	Methylation	SATSLVFKQKTITSW CCEEEEEECCCCCCH	43.32	115974639
77	Methylation	TSLVFKQKTITSWMD EEEEEECCCCCCHHH	42.35	115974647
82	Ubiquitination	KQKTITSWMDTKGIK ECCCCCCHHHCCCCC	4.68	22817900
86	Ubiquitination	ITSWMDTKGIKTAES CCCHHHCCCCCCHHH	55.14	22817900
87	Ubiquitination	TSWMDTKGIKTAESE CCHHHCCCCCCHHHH	28.81	21890473
91	Phosphorylation	DTKGIKTAESESLDS HCCCCCCHHHHCCCC	17.20	33259812
95	Ubiquitination	IKTAESESLDSKENN CCCHHHHCCCCCCCC	46.91	29967540
104	Phosphorylation	DSKENNNTRIESMMS CCCCCCCHHHHHHHH	34.84	28555341
108	Phosphorylation	NNNTRIESMMSSVQK CCCHHHHHHHHHHHH	19.88	23532336
111	Phosphorylation	TRIESMMSSVQKDNF HHHHHHHHHHHHCCC	20.79	24144214
112	Phosphorylation	RIESMMSSVQKDNFY HHHHHHHHHHHCCCH	15.63	30576142
115	Ubiquitination	SMMSSVQKDNFYQHN HHHHHHHHCCCHHHC	53.11	29967540
117	Phosphorylation	MSSVQKDNFYQHNVE HHHHHHCCCHHHCHH	44.56	33259812
119	Phosphorylation	SVQKDNFYQHNVEKL HHHHCCCHHHCHHHH	18.34	28796482
130	Phosphorylation	VEKLENVSQLSLDKS HHHHHCHHHHCCCCC	36.66	23927012
132	Ubiquitination	KLENVSQLSLDKSPT HHHCHHHHCCCCCCC	4.23	21890473
133	Phosphorylation	LENVSQLSLDKSPTE HHCHHHHCCCCCCCH	27.21	23927012
137	Phosphorylation	SQLSLDKSPTEKSTQ HHHCCCCCCCHHHHH	36.40	29255136
139	Phosphorylation	LSLDKSPTEKSTQYL HCCCCCCCHHHHHHH	64.24	29255136
140	Ubiquitination	SLDKSPTEKSTQYLN CCCCCCCHHHHHHHH	48.68	29967540
142	Phosphorylation	DKSPTEKSTQYLNQH CCCCCHHHHHHHHHH	17.95	26074081
143	Phosphorylation	KSPTEKSTQYLNQHQ CCCCHHHHHHHHHHH	32.09	26074081
145	Phosphorylation	PTEKSTQYLNQHQTA CCHHHHHHHHHHHHH	14.01	26074081
151	Phosphorylation	QYLNQHQTAAMCKWQ HHHHHHHHHHHHHHH	17.79	26074081
164	Phosphorylation	WQNEGKHTEQLLESE HHCCCCCHHHHHHCC	28.98	20363803
170	Phosphorylation	HTEQLLESEPQTVTL CHHHHHHCCCCEEEE	55.32	21406692
174	Phosphorylation	LLESEPQTVTLVPEQ HHHCCCCEEEECCHH	26.61	21406692
174	Ubiquitination	LLESEPQTVTLVPEQ HHHCCCCEEEECCHH	26.61	21890473
176	Phosphorylation	ESEPQTVTLVPEQFS HCCCCEEEECCHHHC	25.98	21406692
183	Phosphorylation	TLVPEQFSNANIDRS EECCHHHCCCCCCCC	34.43	21406692
190	Phosphorylation	SNANIDRSPQNDDHS CCCCCCCCCCCCCCC	27.39	30278072
191	Ubiquitination	NANIDRSPQNDDHSD CCCCCCCCCCCCCCC	37.58	21890473
197	Phosphorylation	SPQNDDHSDTDSEEN CCCCCCCCCCCCCCC	49.56	23401153
199	Phosphorylation	QNDDHSDTDSEENRD CCCCCCCCCCCCCCC	44.43	30278072
201	Phosphorylation	DDHSDTDSEENRDNQ CCCCCCCCCCCCCHH	49.23	30278072
212	Phosphorylation	RDNQQFLTTVKLANA CCHHHHHHHHHHHCC	30.72	20363803
213	Phosphorylation	DNQQFLTTVKLANAK CHHHHHHHHHHHCCC	20.14	30576142
223	Phosphorylation	LANAKQTTEDEQARE HHCCCCCCHHHHHHH	38.66	30624053
226	Ubiquitination	AKQTTEDEQAREAKS CCCCCHHHHHHHHHH	39.65	24816145
240	Phosphorylation	SHQKCSKSCDPGEDC HHHHHHHCCCCCCCH	13.88	28634120
240	Ubiquitination	SHQKCSKSCDPGEDC HHHHHHHCCCCCCCH	13.88	29967540
243	Ubiquitination	KCSKSCDPGEDCASC HHHHCCCCCCCHHHC	53.02	24816145
249	Phosphorylation	DPGEDCASCQQDEID CCCCCHHHCCCCCCC	20.84	28634120
257	Ubiquitination	CQQDEIDVVPESPLS CCCCCCCCCCCCCCH	10.72	29967540
261	Phosphorylation	EIDVVPESPLSDVGS CCCCCCCCCCHHCCC	25.68	28348404
264	Phosphorylation	VVPESPLSDVGSEDV CCCCCCCHHCCCCCC	33.37	28348404
268	Phosphorylation	SPLSDVGSEDVGTGP CCCHHCCCCCCCCCC	30.39	26074081
273	Phosphorylation	VGSEDVGTGPKNDNK CCCCCCCCCCCCCCC	50.59	26074081
276	Ubiquitination	EDVGTGPKNDNKLTR CCCCCCCCCCCCCCC	77.58	24816145
282	Phosphorylation	PKNDNKLTRQESCLG CCCCCCCCCCHHHCC	31.73	22199227
286	Phosphorylation	NKLTRQESCLGNSPP CCCCCCHHHCCCCCC	13.30	19664994
291	Phosphorylation	QESCLGNSPPFEKES CHHHCCCCCCCCCCC	31.95	30266825
298	Phosphorylation	SPPFEKESEPESPMD CCCCCCCCCCCCCCC	70.31	25159151
302	Phosphorylation	EKESEPESPMDVDNS CCCCCCCCCCCCCCC	35.92	25159151
309	Phosphorylation	SPMDVDNSKNSCQDS CCCCCCCCCCCCCCC	28.84	23927012
312	Phosphorylation	DVDNSKNSCQDSEAD CCCCCCCCCCCCCCC	19.52	30278072
316	Phosphorylation	SKNSCQDSEADEETS CCCCCCCCCCCCCCC	14.60	30278072
322	Phosphorylation	DSEADEETSPGFDEQ CCCCCCCCCCCCCCC	37.87	23927012
323	Phosphorylation	SEADEETSPGFDEQE CCCCCCCCCCCCCCC	26.72	30278072
323	Ubiquitination	SEADEETSPGFDEQE CCCCCCCCCCCCCCC	26.72	29967540
333	Phosphorylation	FDEQEDGSSSQTANK CCCCCCCCCCCCCCC	38.85	23927012
334	Phosphorylation	DEQEDGSSSQTANKP CCCCCCCCCCCCCCC	31.81	30576142
335	Phosphorylation	EQEDGSSSQTANKPS CCCCCCCCCCCCCCC	32.99	23927012
337	Phosphorylation	EDGSSSQTANKPSRF CCCCCCCCCCCCCHH	33.86	30576142
340	Acetylation	SSSQTANKPSRFQAR CCCCCCCCCCHHCCC	41.74	-
340	Phosphorylation	SSSQTANKPSRFQAR CCCCCCCCCCHHCCC	41.74	32645325
342	Phosphorylation	SQTANKPSRFQARDA CCCCCCCCHHCCCCC	47.54	23927012
349	Ubiquitination	SRFQARDADIEFRKR CHHCCCCCCCHHHHH	17.71	29967540
366	Phosphorylation	TKGGEVRLHFQFEGG CCCCEEEEEEEEECC	5.99	32645325
375	Phosphorylation	FQFEGGESRTGMNDL EEEECCCCCCCCCHH	38.12	25159151
385	Ubiquitination	GMNDLNAKLPGNISS CCCHHCCCCCCCHHH	54.89	-
391	Phosphorylation	AKLPGNISSLNVECR CCCCCCHHHCEEEEC	32.54	28555341
395	Acetylation	GNISSLNVECRNSKQ CCHHHCEEEECCCCC	9.97	19608861
401	Ubiquitination	NVECRNSKQHGKKDS EEEECCCCCCCCCCC	50.27	29967540
421	Acetylation	FMRLPKAEDRRKEQW HHHCCCHHHHHHHHH	59.17	19608861
427	Ubiquitination	AEDRRKEQWETKHQR HHHHHHHHHHHHHHH	46.47	29967540
430	Phosphorylation	RRKEQWETKHQRTER HHHHHHHHHHHHHHC	30.76	28258704
431	Ubiquitination	RKEQWETKHQRTERK HHHHHHHHHHHHHCC	26.65	29967540
438	Ubiquitination	KHQRTERKIPKYVPP HHHHHHCCCCCCCCC	58.07	21890473
438 (in isoform 3)	Ubiquitination	-	58.07	21890473
442	Phosphorylation	TERKIPKYVPPHLSP HHCCCCCCCCCCCCC	16.91	23403867
446	Ubiquitination	IPKYVPPHLSPDKKW CCCCCCCCCCCCCCC	32.63	29967540
448	Phosphorylation	KYVPPHLSPDKKWLG CCCCCCCCCCCCCCC	28.19	25159151
464	Ubiquitination	PIEEMRRMPRCGIRL CHHHHHCCCCCCCCC	1.41	21890473
464 (in isoform 2)	Ubiquitination	-	1.41	21890473
472	Ubiquitination	PRCGIRLPLLRPSAN CCCCCCCCEECCCCC	21.35	29967540
483	Phosphorylation	PSANHTVTIRVDLLR CCCCCEEEEEEEEHH	12.29	24719451
503	Acetylation	KPFPTHYKDLWDNKH CCCCCCHHHCCCCCC	38.45	23749302
505	Ubiquitination	FPTHYKDLWDNKHVK CCCCHHHCCCCCCCC	5.60	21890473
509	Acetylation	YKDLWDNKHVKMPCS HHHCCCCCCCCCCCC	47.50	25953088
509	Ubiquitination	YKDLWDNKHVKMPCS HHHCCCCCCCCCCCC	47.50	29967540
510	Acetylation	KDLWDNKHVKMPCSE HHCCCCCCCCCCCCC	30.59	19413330
512	Acetylation	LWDNKHVKMPCSEQN CCCCCCCCCCCCCCC	36.52	25953088
536	Acetylation	ERTAGSRWELIQTAL CCCCCHHHHHHHHHH	13.37	19413330
541	Phosphorylation	SRWELIQTALLNKFT HHHHHHHHHHHHCCC	16.57	-
546	Ubiquitination	IQTALLNKFTRPQNL HHHHHHHCCCCCCCH	48.35	21890473
546	Ubiquitination	IQTALLNKFTRPQNL HHHHHHHCCCCCCCH	48.35	21890473
546 (in isoform 1)	Ubiquitination	-	48.35	21890473
548	Phosphorylation	TALLNKFTRPQNLKD HHHHHCCCCCCCHHH	42.95	-
554	Ubiquitination	FTRPQNLKDAILKYN CCCCCCHHHHHHHCH	53.30	29967540
582	Ubiquitination	DFWDKVLEEAEAQHL HHHHHHHHHHHHHHH	59.14	24816145
608	Ubiquitination	ALCLPNICTQPIPLL HHHCCCCCCCCHHHH	3.53	24816145
616	Methylation	TQPIPLLKQKMNHSI CCCHHHHHHHHCCCC	56.40	115974631
616	Ubiquitination	TQPIPLLKQKMNHSI CCCHHHHHHHHCCCC	56.40	-
618	Acetylation	PIPLLKQKMNHSITM CHHHHHHHHCCCCCC	39.83	19413330
618	Ubiquitination	PIPLLKQKMNHSITM CHHHHHHHHCCCCCC	39.83	-
622	Phosphorylation	LKQKMNHSITMSQEQ HHHHHCCCCCCCHHH	18.10	26074081
624	Phosphorylation	QKMNHSITMSQEQIA HHHCCCCCCCHHHHH	17.49	26074081
626	Phosphorylation	MNHSITMSQEQIASL HCCCCCCCHHHHHHH	23.16	26074081
632	Phosphorylation	MSQEQIASLLANAFF CCHHHHHHHHHHHHH	26.08	19413330
649	Ubiquitination	FPRRNAKMKSEYSSY CCCCCCCCCHHHCCC	5.54	24816145
650	Ubiquitination	PRRNAKMKSEYSSYP CCCCCCCCHHHCCCC	39.15	-
651	Phosphorylation	RRNAKMKSEYSSYPD CCCCCCCHHHCCCCC	37.96	25072903
653	Phosphorylation	NAKMKSEYSSYPDIN CCCCCHHHCCCCCCC	15.26	25072903
654	Phosphorylation	AKMKSEYSSYPDINF CCCCHHHCCCCCCCH	21.24	25072903
656	Phosphorylation	MKSEYSSYPDINFNR CCHHHCCCCCCCHHH	10.05	-
690	Ubiquitination	FRRVTEKKPTGLVTF HHHCCCCCCCCEEEE	41.39	24816145
912	Phosphorylation	SELMRDIYSMHIFLT HHHHHHHHHEEEEEE	12.29	21406692
913	Phosphorylation	ELMRDIYSMHIFLTE HHHHHHHHEEEEEEC	12.29	21406692
919	Phosphorylation	YSMHIFLTERKLTVG HHEEEEEECCCCCHH	24.01	21406692
945	Phosphorylation	EECRNCSTPGPDIKL HHHHCCCCCCCCCCH	34.17	21712546

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of PARG_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PARG_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PARG_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
BRCA1_HUMAN	BRCA1	physical	25252691
BARD1_HUMAN	BARD1	physical	25252691

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PARG_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-503, AND MASS SPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197 AND THR-199, ANDMASS SPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133; SER-137; SER-197AND THR-199, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."; Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.; Anal. Sci. 24:161-166(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137 AND THR-139, ANDMASS SPECTROMETRY.	18187866 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316, AND MASSSPECTROMETRY.	15302935 [Abstract]