dbPTM

OTU7B_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	OTU7B_HUMAN
UniProt AC	Q6GQQ9
Protein Name	OTU domain-containing protein 7B
Gene Name	OTUD7B
Organism	Homo sapiens (Human).
Sequence Length	843
Subcellular Localization	Cytoplasm . Nucleus . Shuttles be cytoplasm and the nucleus in a XPO1/CRM1-dependent manner.
Protein Description	Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (By similarity). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B. [PubMed: 26903241 Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity Mediates deubiquitination of EGFR]
Protein Sequence	MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDSDNVRLASVILSLEVKLHLLHSYMNVKWIPLSSDAQAPLAQPESPTASAGDEPRSTPESGDSDKESVGSSSTSNEGGRRKEKSKRDREKDKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSKGSKPGGVGTGLGGSSGTETLEKKKKNSLKSWKGGKEEAAGDGPVSEKPPAESVGNGGSKYSQEVMQSLSILRTAMQGEGKFIFVGTLKMGHRHQYQEEMIQRYLSDAEERFLAEQKQKEAERKIMNGGIGGGPPPAKKPEPDAREEQPTGPPAESRAMAFSTGYPGDFTIPRPSGGGVHCQEPRRQLAGGPCVGGLPPYATFPRQCPPGRPYPHQDSIPSLEPGSHSKDGLHRGALLPPPYRVADSYSNGYREPPEPDGWAGGLRGLPPTQTKCKQPNCSFYGHPETNNFCSCCYREELRRREREPDGELLVHRF

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MTLDMDAVL ------CCCCHHHHH	31.31	26714015
10	Phosphorylation	LDMDAVLSDFVRSTG CCHHHHHHHHHHHHC	23.54	26714015
30	Ubiquitination	ARDLLEGKNWDVNAA HHHHHCCCCCCHHHH	45.55	29967540
55	Phosphorylation	HAGNLPPSFSEGSGG HCCCCCCCCCCCCCC	39.19	30576142
57	Phosphorylation	GNLPPSFSEGSGGSR CCCCCCCCCCCCCCC	45.44	29396449
60	Phosphorylation	PPSFSEGSGGSRTPE CCCCCCCCCCCCCCC	35.61	29116813
63	Phosphorylation	FSEGSGGSRTPEKGF CCCCCCCCCCCCCCC	36.18	25159151
65	Phosphorylation	EGSGGSRTPEKGFSD CCCCCCCCCCCCCCC	37.18	25159151
76	Phosphorylation	GFSDREPTRPPRPIL CCCCCCCCCCCCCCC	51.56	29496963
93	Ubiquitination	QDDIVQEKRLSRGIS CCHHHHHHHHHHHHC	41.36	21906983
94	Ubiquitination	DDIVQEKRLSRGISH CHHHHHHHHHHHHCH	36.82	22817900
98	Ubiquitination	QEKRLSRGISHASSS HHHHHHHHHCHHHHH	23.52	22817900
100	Phosphorylation	KRLSRGISHASSSIV HHHHHHHCHHHHHHH	18.80	29255136
103	Phosphorylation	SRGISHASSSIVSLA HHHHCHHHHHHHHHH	21.13	21712546
104	Phosphorylation	RGISHASSSIVSLAR HHHCHHHHHHHHHHH	25.52	23911959
105	Phosphorylation	GISHASSSIVSLARS HHCHHHHHHHHHHHH	25.23	26055452
108	Phosphorylation	HASSSIVSLARSHVS HHHHHHHHHHHHHCC	18.03	23403867
115	Phosphorylation	SLARSHVSSNGGGGG HHHHHHCCCCCCCCC	16.80	22798277
134	Ubiquitination	PLEMPICAFQLPDLT CCCCCEEEEECCCCE	8.92	21890473
144	Ubiquitination	LPDLTVYNEDFRSFI CCCCEEECHHHHHHH	37.72	22817900
148	Ubiquitination	TVYNEDFRSFIERDL EEECHHHHHHHHHHH	42.87	22817900
149	Phosphorylation	VYNEDFRSFIERDLI EECHHHHHHHHHHHH	30.48	-
159	Phosphorylation	ERDLIEQSMLVALEQ HHHHHHHHHHHHHHH	10.99	-
184	Ubiquitination	DPTSQRLLPLATTGD CCCCCCEECEEECCC	3.39	21890473
218	Phosphorylation	LMLRKALYALMEKGV HHHHHHHHHHHHHCC	11.53	-
223	Ubiquitination	ALYALMEKGVEKEAL HHHHHHHHCCCHHHH	55.05	21906983
227	Ubiquitination	LMEKGVEKEALKRRW HHHHCCCHHHHHHHH	45.97	22817900
243	Ubiquitination	WQQTQQNKESGLVYT HHHHHHHHHCCCEEC	49.25	-
256	Ubiquitination	YTEDEWQKEWNELIK ECHHHHHHHHHHHHH	66.81	29967540
263	Ubiquitination	KEWNELIKLASSEPR HHHHHHHHHHHCCCC	51.10	21890473
263	Ubiquitination	KEWNELIKLASSEPR HHHHHHHHHHHCCCC	51.10	21890473
371	Phosphorylation	SMEQKENTKEQAVIP CHHHHCCCCCCEEEE	36.73	-
372	Ubiquitination	MEQKENTKEQAVIPL HHHHCCCCCCEEEEC	60.45	32015554
376	Ubiquitination	ENTKEQAVIPLTDSE CCCCCCEEEECCCCC	4.65	23000965
382	Ubiquitination	AVIPLTDSEYKLLPL EEEECCCCCEEEEEE	37.28	23000965
385	Ubiquitination	PLTDSEYKLLPLHFA ECCCCCEEEEEEEEE	39.22	29967540
387	Ubiquitination	TDSEYKLLPLHFAVD CCCCEEEEEEEEEEC	3.57	23000965
397	Ubiquitination	HFAVDPGKGWEWGKD EEEECCCCCCCCCCC	67.26	29967540
426	Ubiquitination	EVKLHLLHSYMNVKW HHHHHHHHHHCCCEE	24.20	23000965
432	Ubiquitination	LHSYMNVKWIPLSSD HHHHCCCEEEECCCC	34.11	23000965
435	Ubiquitination	YMNVKWIPLSSDAQA HCCCEEEECCCCCCC	25.69	23000965
437	Phosphorylation	NVKWIPLSSDAQAPL CCEEEECCCCCCCCC	22.33	30266825
437	Ubiquitination	NVKWIPLSSDAQAPL CCEEEECCCCCCCCC	22.33	23000965
438	Phosphorylation	VKWIPLSSDAQAPLA CEEEECCCCCCCCCC	44.65	30266825
441	Ubiquitination	IPLSSDAQAPLAQPE EECCCCCCCCCCCCC	48.80	23000965
446	Ubiquitination	DAQAPLAQPESPTAS CCCCCCCCCCCCCCC	50.40	23000965
449	Phosphorylation	APLAQPESPTASAGD CCCCCCCCCCCCCCC	33.94	30266825
451	Phosphorylation	LAQPESPTASAGDEP CCCCCCCCCCCCCCC	42.39	30266825
453	Phosphorylation	QPESPTASAGDEPRS CCCCCCCCCCCCCCC	34.95	30266825
460	Phosphorylation	SAGDEPRSTPESGDS CCCCCCCCCCCCCCC	60.06	23927012
461	Phosphorylation	AGDEPRSTPESGDSD CCCCCCCCCCCCCCC	32.15	23927012
464	Phosphorylation	EPRSTPESGDSDKES CCCCCCCCCCCCHHC	49.45	23927012
467	Phosphorylation	STPESGDSDKESVGS CCCCCCCCCHHCCCC	54.85	23927012
471	O-linked_Glycosylation	SGDSDKESVGSSSTS CCCCCHHCCCCCCCC	37.20	23301498
471	Phosphorylation	SGDSDKESVGSSSTS CCCCCHHCCCCCCCC	37.20	23927012
474	Phosphorylation	SDKESVGSSSTSNEG CCHHCCCCCCCCCCC	20.96	23927012
475	Phosphorylation	DKESVGSSSTSNEGG CHHCCCCCCCCCCCC	31.54	23927012
476	Phosphorylation	KESVGSSSTSNEGGR HHCCCCCCCCCCCCC	37.24	23927012
477	Phosphorylation	ESVGSSSTSNEGGRR HCCCCCCCCCCCCCC	37.20	23403867
478	Phosphorylation	SVGSSSTSNEGGRRK CCCCCCCCCCCCCCC	34.53	23403867
501	Phosphorylation	KDKKRADSVANKLGS HHHHHHHHHHHHHHH	23.34	23403867
505	Acetylation	RADSVANKLGSFGKT HHHHHHHHHHHHHHH	44.48	25953088
505	Ubiquitination	RADSVANKLGSFGKT HHHHHHHHHHHHHHH	44.48	23000965
508	Phosphorylation	SVANKLGSFGKTLGS HHHHHHHHHHHHHHH	41.06	25849741
511	Ubiquitination	NKLGSFGKTLGSKLK HHHHHHHHHHHHHHH	38.27	23000965
512	Phosphorylation	KLGSFGKTLGSKLKK HHHHHHHHHHHHHHH	36.41	26434776
514	Ubiquitination	GSFGKTLGSKLKKNM HHHHHHHHHHHHHHC	28.15	23000965
515	Ubiquitination	SFGKTLGSKLKKNMG HHHHHHHHHHHHHCC	37.62	22817900
516	Ubiquitination	FGKTLGSKLKKNMGG HHHHHHHHHHHHCCC	63.63	23000965
517	Ubiquitination	GKTLGSKLKKNMGGL HHHHHHHHHHHCCCC	11.63	22817900
520	Ubiquitination	LGSKLKKNMGGLMHS HHHHHHHHCCCCCCC	32.51	23000965
522	Ubiquitination	SKLKKNMGGLMHSKG HHHHHHCCCCCCCCC	35.16	22817900
525	Ubiquitination	KKNMGGLMHSKGSKP HHHCCCCCCCCCCCC	3.66	23000965
527	Phosphorylation	NMGGLMHSKGSKPGG HCCCCCCCCCCCCCC	25.42	29449344
530	Phosphorylation	GLMHSKGSKPGGVGT CCCCCCCCCCCCCCC	39.14	30175587
531	Ubiquitination	LMHSKGSKPGGVGTG CCCCCCCCCCCCCCC	57.24	29967540
536	Ubiquitination	GSKPGGVGTGLGGSS CCCCCCCCCCCCCCC	19.83	22817900
537	Phosphorylation	SKPGGVGTGLGGSSG CCCCCCCCCCCCCCC	27.16	27732954
537	Ubiquitination	SKPGGVGTGLGGSSG CCCCCCCCCCCCCCC	27.16	22817900
542	Phosphorylation	VGTGLGGSSGTETLE CCCCCCCCCCCHHHH	24.64	29255136
543	Phosphorylation	GTGLGGSSGTETLEK CCCCCCCCCCHHHHH	53.67	29255136
545	Phosphorylation	GLGGSSGTETLEKKK CCCCCCCCHHHHHHH	27.95	29255136
547	Phosphorylation	GGSSGTETLEKKKKN CCCCCCHHHHHHHHC	39.80	29255136
555	Phosphorylation	LEKKKKNSLKSWKGG HHHHHHCCCCCCCCC	46.16	24719451
565	Ubiquitination	SWKGGKEEAAGDGPV CCCCCCCCCCCCCCC	47.51	22817900
567	Ubiquitination	KGGKEEAAGDGPVSE CCCCCCCCCCCCCCC	21.64	22817900
572	Ubiquitination	EAAGDGPVSEKPPAE CCCCCCCCCCCCCHH	16.45	22817900
574	Ubiquitination	AGDGPVSEKPPAESV CCCCCCCCCCCHHHC	70.58	22817900
576	Ubiquitination	DGPVSEKPPAESVGN CCCCCCCCCHHHCCC	29.31	22817900
581	Ubiquitination	EKPPAESVGNGGSKY CCCCHHHCCCCCCHH	5.34	22817900
586	Ubiquitination	ESVGNGGSKYSQEVM HHCCCCCCHHHHHHH	29.80	22817900
587	Ubiquitination	SVGNGGSKYSQEVMQ HCCCCCCHHHHHHHH	52.80	22817900
589	Phosphorylation	GNGGSKYSQEVMQSL CCCCCHHHHHHHHHH	24.49	22210691
595	Phosphorylation	YSQEVMQSLSILRTA HHHHHHHHHHHHHHH	13.14	22210691
595	Ubiquitination	YSQEVMQSLSILRTA HHHHHHHHHHHHHHH	13.14	22817900
596	Ubiquitination	SQEVMQSLSILRTAM HHHHHHHHHHHHHHH	1.76	22817900
631	Phosphorylation	QEEMIQRYLSDAEER HHHHHHHHHHHHHHH	8.32	29449344
633	Phosphorylation	EMIQRYLSDAEERFL HHHHHHHHHHHHHHH	26.32	28857561
644	Ubiquitination	ERFLAEQKQKEAERK HHHHHHHHHHHHHHH	55.64	21906983
646	Ubiquitination	FLAEQKQKEAERKIM HHHHHHHHHHHHHHH	66.96	22817900
651	Ubiquitination	KQKEAERKIMNGGIG HHHHHHHHHHCCCCC	37.50	22817900
653	Ubiquitination	KEAERKIMNGGIGGG HHHHHHHHCCCCCCC	4.23	22817900
655	Ubiquitination	AERKIMNGGIGGGPP HHHHHHCCCCCCCCC	16.24	22817900
660	Ubiquitination	MNGGIGGGPPPAKKP HCCCCCCCCCCCCCC	26.74	22817900
665	Ubiquitination	GGGPPPAKKPEPDAR CCCCCCCCCCCCCCC	74.71	22817900
666	Ubiquitination	GGPPPAKKPEPDARE CCCCCCCCCCCCCCC	57.63	21906983
674	Ubiquitination	PEPDAREEQPTGPPA CCCCCCCCCCCCCCH	57.20	22817900
675	Ubiquitination	EPDAREEQPTGPPAE CCCCCCCCCCCCCHH	35.78	22817900
677	Phosphorylation	DAREEQPTGPPAESR CCCCCCCCCCCHHHH	63.11	23090842
683	Phosphorylation	PTGPPAESRAMAFST CCCCCHHHHHCCCCC	27.69	23090842
700	Methylation	PGDFTIPRPSGGGVH CCCCCCCCCCCCCCC	33.88	-
702	Phosphorylation	DFTIPRPSGGGVHCQ CCCCCCCCCCCCCCC	51.88	28555341
727	Phosphorylation	CVGGLPPYATFPRQC CCCCCCCCCCCCCCC	19.14	27642862
729	Phosphorylation	GGLPPYATFPRQCPP CCCCCCCCCCCCCCC	28.47	28348404
740	Phosphorylation	QCPPGRPYPHQDSIP CCCCCCCCCCCCCCC	16.40	27080861
745	Phosphorylation	RPYPHQDSIPSLEPG CCCCCCCCCCCCCCC	29.81	23312004
748	Phosphorylation	PHQDSIPSLEPGSHS CCCCCCCCCCCCCCC	43.63	23312004
753	Phosphorylation	IPSLEPGSHSKDGLH CCCCCCCCCCCCCCC	34.99	28555341
769	Phosphorylation	GALLPPPYRVADSYS CCCCCCCCEECCCCC	24.40	28674419
775	Phosphorylation	PYRVADSYSNGYREP CCEECCCCCCCCCCC	13.50	27642862
779	Phosphorylation	ADSYSNGYREPPEPD CCCCCCCCCCCCCCC	18.96	22817900
808	Phosphorylation	KCKQPNCSFYGHPET CCCCCCCCCCCCCCC	28.30	22210691
810	Phosphorylation	KQPNCSFYGHPETNN CCCCCCCCCCCCCCC	9.76	22210691
823	Phosphorylation	NNFCSCCYREELRRR CCCCHHHHHHHHHHH	25.95	22210691

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of OTU7B_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of OTU7B_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of OTU7B_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NHRF2_HUMAN	SLC9A3R2	physical	19615732
NU155_HUMAN	NUP155	physical	19615732
ACAD9_HUMAN	ACAD9	physical	19615732
HIF1N_HUMAN	HIF1AN	physical	19615732
PARK7_HUMAN	PARK7	physical	21097510
EGFR_HUMAN	EGFR	physical	22179831
UBC_HUMAN	UBC	physical	20622874
UBE2S_HUMAN	UBE2S	physical	20622874
XPO1_HUMAN	XPO1	physical	21888622
UBC_HUMAN	UBC	physical	23827681
P55G_HUMAN	PIK3R3	physical	25814554
UBC_HUMAN	UBC	physical	25752577
UBC_HUMAN	UBC	physical	25723849
AURKA_HUMAN	AURKA	physical	26446837
UBC_HUMAN	UBC	physical	26876099
UBC_HUMAN	UBC	physical	27732584

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of OTU7B_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464 AND SER-467, ANDMASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, AND MASSSPECTROMETRY.	18220336 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-449, AND MASSSPECTROMETRY.	15302935 [Abstract]