dbPTM

NSF1C_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NSF1C_RAT
UniProt AC	O35987
Protein Name	NSFL1 cofactor p47
Gene Name	Nsfl1c
Organism	Rattus norvegicus (Rat).
Sequence Length	370
Subcellular Localization	Nucleus . Golgi apparatus, Golgi stack . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Predominantly nuclear in interphase cells (PubMed:12810701). Bound to the axial elements of sex chromosomes in pachytene sperma
Protein Description	Reduces the ATPase activity of VCP. [PubMed: 9824302]
Protein Sequence	MAEERQDALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEEEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGERRRHSGQDVHVVLKLWKTGFSLDNGDLRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLNTSSPAQQAENEAKASSSILINEAEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFVLMTTFPNKELADENQTLKEANLLNAVIVQRLT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
65	Phosphorylation	PSSVSRGTAPSDNRV CCCCCCCCCCCCCCC	33.99	23984901
68	Phosphorylation	VSRGTAPSDNRVTSF CCCCCCCCCCCCCCH	45.08	23984901
73	Phosphorylation	APSDNRVTSFRDLIH CCCCCCCCCHHHHHC	20.93	23984901
74	Phosphorylation	PSDNRVTSFRDLIHD CCCCCCCCHHHHHCC	18.54	23984901
95	Phosphorylation	EEEGQRFYAGGSERS HHHCCCCCCCCCCCC	13.41	23984901
99	Phosphorylation	QRFYAGGSERSGQQI CCCCCCCCCCCCCCC	28.99	23984901
102	Phosphorylation	YAGGSERSGQQIVGP CCCCCCCCCCCCCCC	36.58	-
114	Phosphorylation	VGPPRKKSPNELVDD CCCCCCCCCCHHHHH	35.67	23712012
124	Acetylation	ELVDDLFKGAKEHGA HHHHHHHHHHHHHCC	66.32	22902405
127	Ubiquitination	DDLFKGAKEHGAVAV HHHHHHHHHHCCEEE	59.93	-
138	Phosphorylation	AVAVERVTKSPGETS CEEEEEECCCCCCCC	31.84	28432305
140	Phosphorylation	AVERVTKSPGETSKP EEEEECCCCCCCCCC	28.86	29779826
144	Phosphorylation	VTKSPGETSKPRPFA ECCCCCCCCCCCCCC	48.22	28432305
145	Phosphorylation	TKSPGETSKPRPFAG CCCCCCCCCCCCCCC	36.04	28432305
146	Ubiquitination	KSPGETSKPRPFAGG CCCCCCCCCCCCCCC	53.09	-
146	Acetylation	KSPGETSKPRPFAGG CCCCCCCCCCCCCCC	53.09	22902405
167	Phosphorylation	APEEESAYVAGERRR CCHHHHCCCCCCCCC	10.50	22276854
176	Phosphorylation	AGERRRHSGQDVHVV CCCCCCCCCCCHHHE	36.02	23712012
189	Phosphorylation	VVLKLWKTGFSLDNG HEEEHHHCCCCCCCC	31.70	27097102
192	Phosphorylation	KLWKTGFSLDNGDLR EHHHCCCCCCCCCCC	35.84	27097102
245	Acetylation	HRDEDFVKPKGAFKA CCCCCCCCCCCHHEE	40.79	22902405
251	Acetylation	VKPKGAFKAFTGEGQ CCCCCHHEECCCCCC	41.90	22902405
270	Phosphorylation	TAPQVLNTSSPAQQA CCCCCCCCCCHHHHH	26.81	27097102
271	Phosphorylation	APQVLNTSSPAQQAE CCCCCCCCCHHHHHH	33.00	27097102
272	Phosphorylation	PQVLNTSSPAQQAEN CCCCCCCCHHHHHHH	23.40	23589303
285	Phosphorylation	ENEAKASSSILINEA HHHHHHCCCEEEECC	26.68	26022182
286	Phosphorylation	NEAKASSSILINEAE HHHHHCCCEEEECCC	20.93	26022182
295	Phosphorylation	LINEAEPTTNIQIRL EEECCCCCCEEEEEE	24.79	26022182
311	Ubiquitination	DGGRLVQKFNHSHRI CCCCEEEECCCCCCC	40.43	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
140	S	Phosphorylation	Kinase	CDK1	P39951	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NSF1C_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NSF1C_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
NPL4_RAT	Nploc4	physical	10811609
TERA_RAT	Vcp	physical	10811609

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NSF1C_RAT

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomics of vasopressin-sensitive renal cells:regulation of aquaporin-2 phosphorylation at two sites."; Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.; Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND SER-176, ANDMASS SPECTROMETRY.	16641100 [Abstract]
"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."; Moser K., White F.M.; J. Proteome Res. 5:98-104(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, AND MASSSPECTROMETRY.	16396499 [Abstract]