dbPTM

NOS1_RAT - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NOS1_RAT
UniProt AC	P29476
Protein Name	Nitric oxide synthase, brain
Gene Name	Nos1
Organism	Rattus norvegicus (Rat).
Sequence Length	1429
Subcellular Localization	Cell membrane, sarcolemma Peripheral membrane protein. Cell projection, dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity).
Protein Description	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum..
Protein Sequence	MEENTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPSASKDQSLAVDRVTGLGNGPQHAQGHGQGAGSVSQANGVAIDPTMKSTKANLQDIGEHDELLKEIEPVLSILNSGSKATNRGGPAKAEMKDTGIQVDRDLDGKSHKAPPLGGDNDRVFNDLWGKDNVPVILNNPYSEKEQSPTSGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKPNNSLISNDRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKFSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDADEVFSS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
24	Acetylation	SVRLFKRKVGGLGFL EEEEEEEECCCEEEE	45.91	4918017
24	Ubiquitination	SVRLFKRKVGGLGFL EEEEEEEECCCEEEE	45.91	-
33	Acetylation	GGLGFLVKERVSKPP CCEEEEEECCCCCCC	41.78	4918023
38	Acetylation	LVKERVSKPPVIISD EEECCCCCCCEEEHH	50.95	4918029
77	Phosphorylation	RPLVDLSYDSALEVL EECCCCCHHHHHHHH	22.63	-
119	O-linked_Glycosylation	TGDGTPKTIRVTQPL CCCCCCCEEEEEECC	18.41	28238085
131	Acetylation	QPLGPPTKAVDLSHQ ECCCCCCCEECCCCC	52.79	4918047
143	Ubiquitination	SHQPSASKDQSLAVD CCCCCCCCCCCCHHE	60.55	-
188	Ubiquitination	DPTMKSTKANLQDIG CCCCCCCCCCHHHHH	42.22	-
225	Ubiquitination	TNRGGPAKAEMKDTG CCCCCCCCCHHHCCC	48.27	-
229	Acetylation	GPAKAEMKDTGIQVD CCCCCHHHCCCCCCC	43.58	4918011
229	Ubiquitination	GPAKAEMKDTGIQVD CCCCCHHHCCCCCCC	43.58	-
245	Acetylation	DLDGKSHKAPPLGGD CCCCCCCCCCCCCCC	70.45	4918035
245	Ubiquitination	DLDGKSHKAPPLGGD CCCCCCCCCCCCCCC	70.45	-
280	Phosphorylation	PYSEKEQSPTSGKQS CCCCCCCCCCCCCCC	31.10	22673903
282	Phosphorylation	SEKEQSPTSGKQSPT CCCCCCCCCCCCCCC	56.19	22673903
283	Phosphorylation	EKEQSPTSGKQSPTK CCCCCCCCCCCCCCC	47.58	22673903
283	O-linked_Glycosylation	EKEQSPTSGKQSPTK CCCCCCCCCCCCCCC	47.58	28238085
287	Phosphorylation	SPTSGKQSPTKNGSP CCCCCCCCCCCCCCC	37.71	22673903
289	Phosphorylation	TSGKQSPTKNGSPSR CCCCCCCCCCCCCCC	42.25	22673903
293	Phosphorylation	QSPTKNGSPSRCPRF CCCCCCCCCCCCCCE	29.15	-
295	Phosphorylation	PTKNGSPSRCPRFLK CCCCCCCCCCCCEEE	48.49	28551015
370	Ubiquitination	DQYYSSIKRFGSKAH HHHHHHHHHHCCHHH	43.55	-
374	Phosphorylation	SSIKRFGSKAHMDRL HHHHHHCCHHHHHHH	24.77	1721813
386	Ubiquitination	DRLEEVNKEIESTST HHHHHHHHHHHCCCE	66.30	-
722	Phosphorylation	VWKGTNGTPTKRRAI EECCCCCCCCCCHHH	29.83	25403869
724	Phosphorylation	KGTNGTPTKRRAIGF CCCCCCCCCCHHHCH	36.70	25403869
739	Ubiquitination	KKLAEAVKFSAKLMG HHHHHHHHHHHHHHH	40.42	-
741	Phosphorylation	LAEAVKFSAKLMGQA HHHHHHHHHHHHHHH	20.83	15251453
743	Ubiquitination	EAVKFSAKLMGQAMA HHHHHHHHHHHHHHH	37.50	-
754	Acetylation	QAMAKRVKATILYAT HHHHHHHHEEEEEEC	43.79	4918041
847	Phosphorylation	SYKVRFNSVSSYSDS HCEEEECCCCCCCCC	22.21	14722119
849	Phosphorylation	KVRFNSVSSYSDSRK EEEECCCCCCCCCCC	24.31	22673903
850	Phosphorylation	VRFNSVSSYSDSRKS EEECCCCCCCCCCCC	26.88	22673903
851	Phosphorylation	RFNSVSSYSDSRKSS EECCCCCCCCCCCCC	14.70	22673903
852	Phosphorylation	FNSVSSYSDSRKSSG ECCCCCCCCCCCCCC	30.93	22673903
854	Phosphorylation	SVSSYSDSRKSSGDG CCCCCCCCCCCCCCC	35.35	22673903
856	Acetylation	SSYSDSRKSSGDGPD CCCCCCCCCCCCCCC	53.58	4918053
857	Phosphorylation	SYSDSRKSSGDGPDL CCCCCCCCCCCCCCH	38.14	22108457
858	Phosphorylation	YSDSRKSSGDGPDLR CCCCCCCCCCCCCHH	43.89	11237702
972	Phosphorylation	KRNKFRLTYVAEAPD HHCCEEEEEEEECCC	16.22	26022182
973	Phosphorylation	RNKFRLTYVAEAPDL HCCEEEEEEEECCCC	11.40	26022182
985	Phosphorylation	PDLTQGLSNVHKKRV CCCCCCCCHHHHHHH	43.56	26022182
989	Acetylation	QGLSNVHKKRVSAAR CCCCHHHHHHHHHHH	37.68	4917999
1296	Phosphorylation	DHIYREETLQAKNKG CHHHHHHHHHHHHHH	21.29	16214135
1300	Acetylation	REETLQAKNKGVFRE HHHHHHHHHHHHHHH	46.35	4918005
1321	Acetylation	REPDRPKKYVQDVLQ CCCCCCHHHHHHHHH	54.02	4918059
1371	Ubiquitination	RIMTQQGKLSEEDAG HHHHHCCCCCHHHHH	44.49	-
1371	Acetylation	RIMTQQGKLSEEDAG HHHHHCCCCCHHHHH	44.49	4918065
1412	Phosphorylation	TNRLRSESIAFIEES HHHHHHCCEEEEHHH	22.06	11038355
1428	Phosphorylation	KDADEVFSS------ CCHHHHHCC------	40.07	22673903
1429	Phosphorylation	DADEVFSS------- CHHHHHCC-------	32.54	22673903

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
293	S	Phosphorylation	Kinase	CDK5	Q00535	PSP
293	S	Phosphorylation	Kinase	CDK5	Q03114	PSP
374	S	Phosphorylation	Kinase	PKA-FAMILY	-	GPS
374	S	Phosphorylation	Kinase	PKA_GROUP	-	PhosphoELM
741	S	Phosphorylation	Kinase	CAMK1	Q63450	GPS
847	S	Phosphorylation	Kinase	RSK2	-	PSP
847	S	Phosphorylation	Kinase	CAMK2A	Q9UQM7	PSP
847	S	Phosphorylation	Kinase	CAMK2A	P11275	PSP
847	S	Phosphorylation	Kinase	CAMK4	P13234	GPS
847	S	Phosphorylation	Kinase	RPS6KA1	Q63531	GPS
847	S	Phosphorylation	Kinase	CAMK1-FAMILY	-	GPS
847	S	Phosphorylation	Kinase	RSK-SUBFAMILY	-	GPS
847	S	Phosphorylation	Kinase	RSK_GROUP	-	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NOS1_RAT !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NOS1_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CAV3_RAT	Cav3	physical	9353265
HSP74_HUMAN	HSPA4	physical	22128174
HS90A_HUMAN	HSP90AA1	physical	22128174
CHIP_HUMAN	STUB1	physical	22128174
NOSIP_HUMAN	NOSIP	physical	15548660
UBC_RAT	Ubc	physical	24186360
KPCD1_RAT	Prkd1	physical	24740233

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NOS1_RAT

Related Literatures of Post-Translational Modification