NGLY1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NGLY1_MOUSE
• UniProt AC: Q9JI78
• Protein Name: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
• Gene Name: Ngly1
• Organism: Mus musculus (Mouse).
• Sequence Length: 651
• Subcellular Localization: Cytoplasm .
• Protein Description: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins..
• Protein Sequence: MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFNDL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MASATLGSS ------CCCCCCCCC	14.10	-
50	Ubiquitination	LRNPSDEKYRSIRIG HHCCCCHHCCCCEEC	51.45	22790023
119	Ubiquitination	RLDGSSKKVQFSQHP CCCCCCCEEEECCCC	43.12	22790023
130	Ubiquitination	SQHPAAAKLPLEQSE CCCCHHCCCCCCCCC	46.00	22790023
155	Phosphorylation	NQTGQLPSLPSAPMV CCCCCCCCCCCCCEE	62.60	28285833
201	Ubiquitination	CIPVSELKRKAQEKL CEEHHHHHHHHHHHH	48.04	22790023
269	Ubiquitination	LPNDDELKWGAKNVE CCCCCCCCCCCCCCH	41.35	22790023
273	Ubiquitination	DELKWGAKNVENHYC CCCCCCCCCCHHHCC	58.55	22790023
378	Acetylation	SYIIAFSKDEVVDVT EEEEEEECCCEEEEE	51.99	15630559
403	Ubiquitination	MSRRTKVKEELLRET HHCHHHHHHHHHHHH	47.21	22790023
416	Ubiquitination	ETINGLNKQRQLSLS HHHHHHHHHHCCCCC	52.48	22790023
421	Phosphorylation	LNKQRQLSLSESRRK HHHHHCCCCCHHHHH	22.85	30387612
474	Ubiquitination	GETGLERKEILFIPS CCCCCCEEEEEEECC	39.47	22790023
485	Ubiquitination	FIPSENEKISKQFHL EECCCCCHHHHHHHH	64.95	27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NGLY1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NGLY1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NGLY1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
AMFR_MOUSE	Amfr	physical	16709668
TERA_MOUSE	Vcp	physical	16709668
PRS4_MOUSE	Psmc1	physical	15358861
RD23B_MOUSE	Rad23b	physical	15358861
RD23B_MOUSE	Rad23b	physical	11562482
RS2_MOUSE	Rps2	physical	11562482
AMFR_MOUSE	Amfr	physical	11562482
UBC_MOUSE	Ubc	physical	11562482
IMA1_MOUSE	Kpna2	physical	11562482
TERA_MOUSE	Vcp	physical	17496150

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.