IMA1_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: IMA1_MOUSE
• UniProt AC: P52293
• Protein Name: Importin subunit alpha-1 {ECO:0000305}
• Gene Name: Kpna2 {ECO:0000312|MGI:MGI:103561}
• Organism: Mus musculus (Mouse).
• Sequence Length: 529
• Subcellular Localization: Cytoplasm . Nucleus .
• Protein Description: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus..
• Protein Sequence: MSTNENANLPAARLNRFKNKGKDSTEMRRRRIEVNVELRKAKKDEQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLSTLACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADSCWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIVTGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCGIIEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQRHENESVYKASLNLIEKYFSVEEEEDQNVVPETTSEGFAFQVQDGAPGTFNF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSTNENANL ------CCCCCCCCC	43.22	-
2	Phosphorylation	------MSTNENANL ------CCCCCCCCC	43.22	22006019
3	Phosphorylation	-----MSTNENANLP -----CCCCCCCCCC	34.46	17242355
24	Phosphorylation	FKNKGKDSTEMRRRR HHHCCCCCHHHHHHH	5.42	27149854
25	Phosphorylation	KNKGKDSTEMRRRRI HHCCCCCHHHHHHHH	27.23	25266776
54	Phosphorylation	MLKRRNVSSFPDDAT HHHHCCCCCCCCCCC	52.60	27149854
55	Phosphorylation	LKRRNVSSFPDDATS HHHCCCCCCCCCCCC	68.69	26824392
61	Phosphorylation	SSFPDDATSPLQENR CCCCCCCCCCCHHHC	5.14	27087446
62	Phosphorylation	SFPDDATSPLQENRN CCCCCCCCCCHHHCC	3.23	25521595
73	Phosphorylation	ENRNNQGTVNWSVED HHCCCCCCCCCCHHH	29.70	23984901
77	Phosphorylation	NQGTVNWSVEDIVKG CCCCCCCCHHHHHHC	3.67	23984901
108	Ubiquitination	RKLLSREKQPPIDNI HHHHHCCCCCCCHHH	57.84	22790023
108	Acetylation	RKLLSREKQPPIDNI HHHHHCCCCCCCHHH	57.84	23806337
126	Phosphorylation	GLIPKFVSFLGKTDC CCHHHHHHHHCCCCC	25.21	22006019
202	Ubiquitination	AFRDLVIKHGAIDPL HHHHHHHHCCCCHHH	1.72	-
240	Ubiquitination	LSNLCRNKNPAPPLD HHHHHCCCCCCCCHH	6.70	22790023
300	Ubiquitination	GVVPQLVKLLGATEL CHHHHHHHHHCCCCC	37.65	-
348	Ubiquitination	PSLLTNPKTNIQKEA HHHHCCCCCCHHHHH	51.45	22790023
459	Ubiquitination	EKLGETEKLSIMIEE HHHCCCHHHHHHHHH	45.72	-
483	Phosphorylation	LQRHENESVYKASLN HHHCCCHHHHHHHHH	9.54	27841257
486	Ubiquitination	HENESVYKASLNLIE CCCHHHHHHHHHHHH	34.61	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of IMA1_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of IMA1_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of IMA1_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LEF1_HUMAN	LEF1	physical	8631802
PCY1A_MOUSE	Pcyt1a	physical	19332566
PLCB1_RAT	Plcb1	physical	23665500
IMA1_MOUSE	Kpna2	physical	23665500
IMB1_MOUSE	Kpnb1	physical	23665500
TIF1B_HUMAN	TRIM28	physical	25960296
CBX1_HUMAN	CBX1	physical	25960296
SART3_HUMAN	SART3	physical	27060135

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale phosphorylation analysis of mouse liver.";
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-3, AND MASSSPECTROMETRY.
PubMed: 17242355