NEP1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NEP1_HUMAN
• UniProt AC: Q92979
• Protein Name: Ribosomal RNA small subunit methyltransferase NEP1 {ECO:0000305|PubMed:20047967}
• Gene Name: EMG1 {ECO:0000303|PubMed:19463982}
• Organism: Homo sapiens (Human).
• Sequence Length: 244
• Subcellular Localization: Nucleus, nucleolus .
• Protein Description: S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. [PubMed: 20047967 Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes (By similarity]
• Protein Sequence: MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAPSDGFK ------CCCCCCCCC	28.25	22814378
5	Phosphorylation	---MAAPSDGFKPRE ---CCCCCCCCCCCC	45.71	29255136
9	Acetylation	AAPSDGFKPRERSGG CCCCCCCCCCCCCCC	49.01	23954790
9	Ubiquitination	AAPSDGFKPRERSGG CCCCCCCCCCCCCCC	49.01	24816145
14	Phosphorylation	GFKPRERSGGEQAQD CCCCCCCCCCCCCCC	46.53	25159151
28	Acetylation	DWDALPPKRPRLGAG CCCCCCCCCCCCCCC	73.43	26051181
28	Ubiquitination	DWDALPPKRPRLGAG CCCCCCCCCCCCCCC	73.43	29967540
37	Acetylation	PRLGAGNKIGGRRLI CCCCCCCCCCCEEEE	41.64	25953088
37	Ubiquitination	PRLGAGNKIGGRRLI CCCCCCCCCCCEEEE	41.64	24816145
51	Phosphorylation	IVVLEGASLETVKVG EEEEECCCEEEEECC	37.28	20068231
54	Phosphorylation	LEGASLETVKVGKTY EECCCEEEEECCCEE	30.81	20068231
56	Ubiquitination	GASLETVKVGKTYEL CCCEEEEECCCEEEE	53.33	23000965
59	Ubiquitination	LETVKVGKTYELLNC EEEEECCCEEEEECC	51.42	23000965
59	2-Hydroxyisobutyrylation	LETVKVGKTYELLNC EEEEECCCEEEEECC	51.42	-
59	Acetylation	LETVKVGKTYELLNC EEEEECCCEEEEECC	51.42	23954790
60	Phosphorylation	ETVKVGKTYELLNCD EEEECCCEEEEECCC	19.12	21815630
61	Phosphorylation	TVKVGKTYELLNCDK EEECCCEEEEECCCC	14.20	28152594
68	Ubiquitination	YELLNCDKHKSILLK EEEECCCCCCEEEEE	55.95	29967540
68	Acetylation	YELLNCDKHKSILLK EEEECCCCCCEEEEE	55.95	23749302
70	Acetylation	LLNCDKHKSILLKNG EECCCCCCEEEEECC	44.95	26051181
70	Ubiquitination	LLNCDKHKSILLKNG EECCCCCCEEEEECC	44.95	29967540
75	Acetylation	KHKSILLKNGRDPGE CCCEEEEECCCCCCC	54.64	25953088
75	Ubiquitination	KHKSILLKNGRDPGE CCCEEEEECCCCCCC	54.64	21890473
91	Phosphorylation	RPDITHQSLLMLMDS CCCCCHHHHHHHHCC	18.83	-
154	Ubiquitination	RAADGPQKLLKVIKN HCCCCHHHHHHHHCC	59.94	23000965
154	Acetylation	RAADGPQKLLKVIKN HCCCCHHHHHHHHCC	59.94	25953088
157	Ubiquitination	DGPQKLLKVIKNPVS CCHHHHHHHHCCCCC	52.96	23000965
157	Acetylation	DGPQKLLKVIKNPVS CCHHHHHHHHCCCCC	52.96	26051181
160	Acetylation	QKLLKVIKNPVSDHF HHHHHHHCCCCCCCC	59.18	26051181
160	Ubiquitination	QKLLKVIKNPVSDHF HHHHHHHCCCCCCCC	59.18	23000965
176	Phosphorylation	VGCMKVGTSFSIPVV CCCEEECCCEEEEEE	29.59	23186163
177	Phosphorylation	GCMKVGTSFSIPVVS CCEEECCCEEEEEEC	15.86	23186163
179	Phosphorylation	MKVGTSFSIPVVSDV EEECCCEEEEEECCH	26.25	23186163
218	Phosphorylation	EYTEKMVSISNYPLS EEEEEEEECCCCCHH	19.46	28152594
220	Phosphorylation	TEKMVSISNYPLSAA EEEEEECCCCCHHHH	23.86	28442448
222	Phosphorylation	KMVSISNYPLSAALT EEEECCCCCHHHHHH	9.96	28152594
225	Phosphorylation	SISNYPLSAALTCAK ECCCCCHHHHHHHHH	13.64	28442448
229	Phosphorylation	YPLSAALTCAKLTTA CCHHHHHHHHHHHHH	12.99	28442448
234	Phosphorylation	ALTCAKLTTAFEEVW HHHHHHHHHHHHHHH	18.51	28122231
235	Phosphorylation	LTCAKLTTAFEEVWG HHHHHHHHHHHHHHC	39.52	28122231

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NEP1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NEP1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NEP1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
S2538_HUMAN	SLC25A38	physical	16169070
KHDR1_HUMAN	KHDRBS1	physical	16169070
A4_HUMAN	APP	physical	21832049
IL7RA_HUMAN	IL7R	physical	23151878

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 211180: Bowen-Conradi syndrome (BWCNS)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Evaluation of the low-specificity protease elastase for large-scalephosphoproteome analysis.";
Wang B., Malik R., Nigg E.A., Korner R.;
Anal. Chem. 80:9526-9533(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5 AND SER-14, AND MASSSPECTROMETRY.
PubMed: 19007248