dbPTM

NACA_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NACA_MOUSE
UniProt AC	Q60817
Protein Name	Nascent polypeptide-associated complex subunit alpha
Gene Name	Naca
Organism	Mus musculus (Mouse).
Sequence Length	215
Subcellular Localization	Cytoplasm. Nucleus. The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus..
Protein Description	Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity). Isoform 1 and isoform 2 appear to bind DNA and play roles in transcription. Isoform 1 may function as a specific coactivator for JUN, acting to stabilize the interaction of JUN homodimers with promoter elements..
Protein Sequence	MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQTATQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Phosphorylation	GSGTESDSDESVPEL CCCCCCCCCCCCCCH	53.18	22802335
34	Phosphorylation	TESDSDESVPELEEQ CCCCCCCCCCCHHHC	48.22	22802335
43	Phosphorylation	PELEEQDSTQTATQQ CCHHHCCCHHHHHHH	23.31	15299025
44	Phosphorylation	ELEEQDSTQTATQQA CHHHCCCHHHHHHHH	37.92	22802335
46	Phosphorylation	EEQDSTQTATQQAQL HHCCCHHHHHHHHHH	31.59	22802335
99	Phosphorylation	TRVTIRKSKNILFVI EEEEEECCCCEEEEE	22.53	-
127	Sumoylation	YIVFGEAKIEDLSQQ EEEEEEEEHHHHHHH	42.18	-
132	Phosphorylation	EAKIEDLSQQAQLAA EEEHHHHHHHHHHHH	32.35	-
142	Acetylation	AQLAAAEKFKVQGEA HHHHHHHHHCCCCCH	45.84	23806337
142	Ubiquitination	AQLAAAEKFKVQGEA HHHHHHHHHCCCCCH	45.84	27667366
159	Phosphorylation	NIQENTQTPTVQEES CHHHHCCCCCCCCHH	20.91	15005626
161	Phosphorylation	QENTQTPTVQEESEE HHHCCCCCCCCHHHH	37.90	15345747
166	Phosphorylation	TPTVQEESEEEEVDE CCCCCCHHHHHCCCC	49.94	15345747
186	Phosphorylation	KDIELVMSQANVSRA HHHHHHHHHHCCHHH	20.75	-
191	Phosphorylation	VMSQANVSRAKAVRA HHHHHCCHHHHHHHH	26.74	-
203	Phosphorylation	VRALKNNSNDIVNAI HHHHHCCCHHHHHHH	45.32	-
2114	Ubiquitination	---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------		27667366

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
43	S	Phosphorylation	Kinase	ILK	O55222	GPS
43	S	Phosphorylation	Kinase	ILK1	-	Uniprot
99	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
159	T	Phosphorylation	Kinase	GSK3B	Q9WV60	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
43	S	Phosphorylation	15299025
Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.
159	T	Phosphorylation	15005626
Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NACA_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TBP_MOUSE	Tbp	physical	9488445
FADD_HUMAN	FADD	physical	24901053
NSE2_MOUSE	Nsmce2	physical	25002400

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NACA_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry."; Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.; J. Proteome Res. 6:250-262(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166, AND MASSSPECTROMETRY.	17203969 [Abstract]
"Phosphoproteomic analysis of the developing mouse brain."; Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.; Mol. Cell. Proteomics 3:1093-1101(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-161 AND SER-166, ANDMASS SPECTROMETRY.	15345747 [Abstract]
"Integrin-linked kinase regulates the nuclear entry of the c-Juncoactivator alpha-NAC and its coactivation potency."; Quelo I., Gauthier C., Hannigan G.E., Dedhar S., St-Arnaud R.; J. Biol. Chem. 279:43893-43899(2004). Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ILK, ANDPHOSPHORYLATION AT SER-43.	15299025 [Abstract]
"GSK3 beta-dependent phosphorylation of the alpha NAC coactivatorregulates its nuclear translocation and proteasome-mediateddegradation."; Quelo I., Akhouayri O., Prud'homme J., St Arnaud R.; Biochemistry 43:2906-2914(2004). Cited for: SUBCELLULAR LOCATION, DEGRADATION, AND PHOSPHORYLATION AT THR-159.	15005626 [Abstract]

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