MYPT2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MYPT2_HUMAN
• UniProt AC: O60237
• Protein Name: Protein phosphatase 1 regulatory subunit 12B
• Gene Name: PPP1R12B
• Organism: Homo sapiens (Human).
• Sequence Length: 982
• Subcellular Localization: Cytoplasm . Along actomyosin filaments and stress fibers.
• Protein Description: Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus..
• Protein Sequence: MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEADKKPEAFVNHSNSESKSSITEQIPAPAQNTFSASSARRFSSGLFNKPEEPKDESPSSWRLGLRKTGSHNMLSEVANSREPIRDRGSSIYRSSSSPRISALLDNKDKERENKSYISSLAPRKLNSTSDIEEKENRESAVNLVRSGSYTRQLWRDEAKGNEIPQTIAPSTYVSTYLKRTPHKSQADTTAEKTADNVSSSTPLCVITNRPLPSTANGVTATPVLSITGTDSSVEAREKRRSYLTPVRDEEAESLRKARSRQARQTRRSTQGVTLTDLQEAERTFSRSRAERQAQEQPREKPTDTEGLEGSPEKHEPSAVPATEAGEGQQPWGRSLDEEPICHRLRCPAQPDKPTTPASPSTSRPSLYTSSHLLWTNRFSVPDSESSETTTNTTTAKEMDKNENEEADLDEQSSKRLSIRERRRPKERRRGTGINFWTKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASARARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKSKLEKVAQQKQEKTSDRSSVLEMEKRERRALERKMSEMEEEMKVLTELKSDNQRLKDENGALIRVISKLSK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Acetylation	ELEHLGGKRAESARM CHHHHCCHHHHHHHH	46.79	25953088
15 (in isoform 3)	Phosphorylation	-	10.78	25159151
15 (in isoform 4)	Phosphorylation	-	10.78	25159151
19	Phosphorylation	AESARMRRAEQLRRW HHHHHHHHHHHHHHH	32.88	27251275
21	Phosphorylation	SARMRRAEQLRRWRG HHHHHHHHHHHHHHC	48.62	24719451
25	Phosphorylation	RRAEQLRRWRGSLTE HHHHHHHHHHCCCCC	35.72	24719451
29	Phosphorylation	QLRRWRGSLTEQEPA HHHHHHCCCCCCCHH	24.44	18212344
31	Phosphorylation	RRWRGSLTEQEPAER HHHHCCCCCCCHHHH	37.00	27251275
51	Phosphorylation	QPLTRRGSPRVRFED CCCCCCCCCCEEECC	14.02	24719451
67	Phosphorylation	AVFLAACSSGDTDEV CEEEEECCCCCHHHH	32.40	-
216	Phosphorylation	EDVRQARSGATALHV HHHHHHHCCCHHHHH	35.48	27732954
241	Phosphorylation	RLLIQAGYELNVQDY HHHHHCCCCCCCCCC	21.91	18083107
248	Phosphorylation	YELNVQDYDGWTPLH CCCCCCCCCCCCHHH	10.16	18083107
303	Ubiquitination	EHLELLQKKQNVLRS HHHHHHHHHHHHHHC	57.19	-
310	Phosphorylation	KKQNVLRSEKETRNK HHHHHHHCCHHHHHH	49.19	23403867
321	Phosphorylation	TRNKLIESDLNSKIQ HHHHHHHHHHHHHHH	40.16	26852163
325	Phosphorylation	LIESDLNSKIQSGFF HHHHHHHHHHHHCCC	38.13	26852163
340	Phosphorylation	KNKEKMLYEEETPKS CCHHHHHCCCCCCCH	20.14	25159151
344	Phosphorylation	KMLYEEETPKSQEME HHHCCCCCCCHHHHH	39.02	29052541
347	Phosphorylation	YEEETPKSQEMEEEN CCCCCCCHHHHHHHH	32.35	-
358	Phosphorylation	EEENKESSSSSSEEE HHHHHHCCCCCCHHH	35.02	-
359	Phosphorylation	EENKESSSSSSEEEE HHHHHCCCCCCHHHC	43.44	-
374	Phosphorylation	GEDEASESETEKEAD CCCCCCCHHHHHHHH	47.53	-
376	Phosphorylation	DEASESETEKEADKK CCCCCHHHHHHHHHC	62.73	-
391	Phosphorylation	PEAFVNHSNSESKSS CHHHHCCCCCCCCCC	36.53	27251275
393	Phosphorylation	AFVNHSNSESKSSIT HHHCCCCCCCCCCCC	46.38	25159151
410	Phosphorylation	IPAPAQNTFSASSAR CCCCCCCCCCHHHHH	13.59	-
412	Phosphorylation	APAQNTFSASSARRF CCCCCCCCHHHHHHH	25.79	25627689
420	Phosphorylation	ASSARRFSSGLFNKP HHHHHHHHCCCCCCC	22.99	20873877
421	Phosphorylation	SSARRFSSGLFNKPE HHHHHHHCCCCCCCC	36.48	20873877
434	Phosphorylation	PEEPKDESPSSWRLG CCCCCCCCCCCHHHH	39.97	21815630
445	Phosphorylation	WRLGLRKTGSHNMLS HHHHHHCCCCHHHHH	37.17	28985074
447	Phosphorylation	LGLRKTGSHNMLSEV HHHHCCCCHHHHHHH	19.54	25159151
452	Phosphorylation	TGSHNMLSEVANSRE CCCHHHHHHHHHCCC	21.18	-
471	Phosphorylation	RGSSIYRSSSSPRIS CCCCHHCCCCCHHHH	20.01	22199227
472	Phosphorylation	GSSIYRSSSSPRISA CCCHHCCCCCHHHHH	26.32	22199227
473	Phosphorylation	SSIYRSSSSPRISAL CCHHCCCCCHHHHHH	45.68	22199227
474	Phosphorylation	SIYRSSSSPRISALL CHHCCCCCHHHHHHH	21.83	22199227
492	Phosphorylation	DKERENKSYISSLAP CHHHCCHHHHHHHCC	39.00	23403867
493	Phosphorylation	KERENKSYISSLAPR HHHCCHHHHHHHCCC	13.36	23403867
495	Phosphorylation	RENKSYISSLAPRKL HCCHHHHHHHCCCCC	15.86	23403867
496	Phosphorylation	ENKSYISSLAPRKLN CCHHHHHHHCCCCCC	20.99	24719451
504	Phosphorylation	LAPRKLNSTSDIEEK HCCCCCCCCCCHHHH	39.46	21815630
505	Phosphorylation	APRKLNSTSDIEEKE CCCCCCCCCCHHHHH	29.37	27251275
506	Phosphorylation	PRKLNSTSDIEEKEN CCCCCCCCCHHHHHC	36.09	23312004
523	Phosphorylation	SAVNLVRSGSYTRQL HHHHHHHCCCHHHHH	25.31	25159151
525	Phosphorylation	VNLVRSGSYTRQLWR HHHHHCCCHHHHHHC	25.28	-
527	Phosphorylation	LVRSGSYTRQLWRDE HHHCCCHHHHHHCCH	17.40	-
543	O-linked_Glycosylation	KGNEIPQTIAPSTYV CCCCCCCCCCHHHHH	17.47	30059200
549	Phosphorylation	QTIAPSTYVSTYLKR CCCCHHHHHHHHHHC	9.20	19658100
561	Phosphorylation	LKRTPHKSQADTTAE HHCCCCHHHCCCCCH	27.80	-
562 (in isoform 6)	Phosphorylation	-	48.32	23090842
563 (in isoform 6)	Phosphorylation	-	24.17	23090842
565	Phosphorylation	PHKSQADTTAEKTAD CCHHHCCCCCHHHCC	30.81	-
566 (in isoform 6)	Phosphorylation	-	33.11	23090842
567 (in isoform 6)	Phosphorylation	-	20.55	21815630
569 (in isoform 6)	Phosphorylation	-	46.69	23090842
571 (in isoform 6)	Phosphorylation	-	19.90	23090842
576 (in isoform 6)	Phosphorylation	-	33.67	22199227
577 (in isoform 6)	Phosphorylation	-	26.91	22199227
580 (in isoform 6)	Phosphorylation	-	3.54	23090842
581 (in isoform 6)	Phosphorylation	-	1.88	22199227
584 (in isoform 6)	Phosphorylation	-	12.55	23090842
587 (in isoform 6)	Phosphorylation	-	51.17	23090842
588 (in isoform 6)	Phosphorylation	-	4.77	23090842
589 (in isoform 6)	Phosphorylation	-	32.11	23090842
590 (in isoform 6)	Phosphorylation	-	48.08	23090842
618	Phosphorylation	EAREKRRSYLTPVRD HHHHHHHHHCCCCCH	28.64	24702127
619	Phosphorylation	AREKRRSYLTPVRDE HHHHHHHHCCCCCHH	16.88	24702127
621	Phosphorylation	EKRRSYLTPVRDEEA HHHHHHCCCCCHHHH	16.11	25159151
630	Phosphorylation	VRDEEAESLRKARSR CCHHHHHHHHHHHHH	40.29	27461979
642	Phosphorylation	RSRQARQTRRSTQGV HHHHHHHHHHHCCCC	23.61	-
645	Phosphorylation	QARQTRRSTQGVTLT HHHHHHHHCCCCCHH	23.07	21712546
646	Phosphorylation	ARQTRRSTQGVTLTD HHHHHHHCCCCCHHH	27.77	28348404
650	Phosphorylation	RRSTQGVTLTDLQEA HHHCCCCCHHHHHHH	30.66	23403867
652	Phosphorylation	STQGVTLTDLQEAER HCCCCCHHHHHHHHH	25.87	23403867
679	Phosphorylation	EQPREKPTDTEGLEG HCCCCCCCCCCCCCC	67.62	26657352
681	Phosphorylation	PREKPTDTEGLEGSP CCCCCCCCCCCCCCC	34.14	29255136
687	Phosphorylation	DTEGLEGSPEKHEPS CCCCCCCCCCCCCCC	22.10	29255136
694	Phosphorylation	SPEKHEPSAVPATEA CCCCCCCCCCCCCCC	38.62	28464451
699	Phosphorylation	EPSAVPATEAGEGQQ CCCCCCCCCCCCCCC	21.94	20873877
711	Phosphorylation	GQQPWGRSLDEEPIC CCCCCCCCCCCCCCH	35.91	26471730
731	Phosphorylation	PAQPDKPTTPASPST CCCCCCCCCCCCCCC	51.67	27732954
732	Phosphorylation	AQPDKPTTPASPSTS CCCCCCCCCCCCCCC	25.94	27732954
735	Phosphorylation	DKPTTPASPSTSRPS CCCCCCCCCCCCCCC	22.16	27732954
737	Phosphorylation	PTTPASPSTSRPSLY CCCCCCCCCCCCCCE	36.20	27732954
738	Phosphorylation	TTPASPSTSRPSLYT CCCCCCCCCCCCCEE	31.94	27732954
739	Phosphorylation	TPASPSTSRPSLYTS CCCCCCCCCCCCEEC	46.67	27732954
742	Phosphorylation	SPSTSRPSLYTSSHL CCCCCCCCCEECCCE	32.66	29083192
744	Phosphorylation	STSRPSLYTSSHLLW CCCCCCCEECCCEEE	14.65	29083192
745	Phosphorylation	TSRPSLYTSSHLLWT CCCCCCEECCCEEEE	29.10	29083192
746	Phosphorylation	SRPSLYTSSHLLWTN CCCCCEECCCEEEEC	11.38	29083192
747	Phosphorylation	RPSLYTSSHLLWTNR CCCCEECCCEEEECC	15.45	29083192
752	Phosphorylation	TSSHLLWTNRFSVPD ECCCEEEECCCCCCC	19.24	29083192
760	Phosphorylation	NRFSVPDSESSETTT CCCCCCCCCCCCCCC	33.05	-
762	Phosphorylation	FSVPDSESSETTTNT CCCCCCCCCCCCCCC	37.11	-
789	Phosphorylation	EADLDEQSSKRLSIR CCCCCHHHHHHHHHH	35.46	25159151
790	Phosphorylation	ADLDEQSSKRLSIRE CCCCHHHHHHHHHHH	22.84	25159151
794	Phosphorylation	EQSSKRLSIRERRRP HHHHHHHHHHHHHCC	24.51	-
808	Phosphorylation	PKERRRGTGINFWTK CHHHHCCCCCCCCCC	32.83	27732954
814	Phosphorylation	GTGINFWTKDEDETD CCCCCCCCCCCCCCC	25.20	23882029
829	Phosphorylation	GSEEVKETWHERLSR CCHHHHHHHHHHHHH	26.80	30576142
835	Phosphorylation	ETWHERLSRLESGGS HHHHHHHHHHHHCCC	40.83	29507054
839	Phosphorylation	ERLSRLESGGSNPTT HHHHHHHHCCCCCCC	53.71	23911959
842	Phosphorylation	SRLESGGSNPTTSDS HHHHHCCCCCCCCCC	43.06	30242111
845	Phosphorylation	ESGGSNPTTSDSYGD HHCCCCCCCCCCHHH	43.09	30576142
846	Phosphorylation	SGGSNPTTSDSYGDR HCCCCCCCCCCHHHH	31.62	23312004
847	Phosphorylation	GGSNPTTSDSYGDRA CCCCCCCCCCHHHHH	27.34	23312004
849	Phosphorylation	SNPTTSDSYGDRASA CCCCCCCCHHHHHHH	30.68	23312004
850	Phosphorylation	NPTTSDSYGDRASAR CCCCCCCHHHHHHHH	28.21	23312004
855	Phosphorylation	DSYGDRASARARREA CCHHHHHHHHHHHHH	21.12	30576142
869	Phosphorylation	AREARLATLTSRVEE HHHHHHHHHHHHHHH	34.85	29449344
871	Phosphorylation	EARLATLTSRVEEDS HHHHHHHHHHHHHCC	15.33	29449344
872	Phosphorylation	ARLATLTSRVEEDSN HHHHHHHHHHHHCCC	37.12	29449344
878	Phosphorylation	TSRVEEDSNRDYKKL HHHHHHCCCHHHHHH	36.72	19664995
947	Phosphorylation	RALERKMSEMEEEMK HHHHHHHHHHHHHHH	36.51	21955146
957	Phosphorylation	EEEMKVLTELKSDNQ HHHHHHHHHHHHHCH	42.11	21955146
960	Acetylation	MKVLTELKSDNQRLK HHHHHHHHHHCHHHC	49.91	25953088
981	Phosphorylation	IRVISKLSK------ HHHHHHHCC------	100.00	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
646	T	Phosphorylation	Kinase	ROCK1	Q13464	PSP

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MYPT2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MYPT2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
IL16_HUMAN	IL16	physical	12923170
PP1B_HUMAN	PPP1CB	physical	9570949
SKP1_HUMAN	SKP1	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.";
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.;
Anal. Sci. 24:161-166(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-947, AND MASSSPECTROMETRY.
PubMed: 18187866